Reviewed, UniProtKB/Swiss-Prot Q68VV2 (NUOG_RICTY)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit G
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit G
    NDH-1 subunit G

Gene names

Name:	nuoG
Ordered Locus Names:	RT0784

Organism

Rickettsia typhi [Complete proteome] [HAMAP]

Taxonomic identifier

785 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 675	675	NADH-quinone oxidoreductase subunit G		PRO_0000287844
Regions
	Domain	1 – 78	78	2Fe-2S ferredoxin-type
Sites
	Metal binding	34	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	45	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	48	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	62	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	94	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	98	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	101	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	107	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	146	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	152	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	196	1	Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68VV2-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 037650EB4680CF4F	FASTA	675	75,554
	10 20 30 40 50 60 MIKLIIDGSE IEISEGSTVY QACIQAGKQI PHFCYHARLK IAGNCRMCLV EIEKSQKPVA 70 80 90 100 110 120 SCAMPVSNGM VIHTDTSMVK KAREGVMEFL LINHPLDCPI CDQGGECDLQ DQAFRYGKGT 130 140 150 160 170 180 NRFHENKRSI KDKYMGPLIK TAMTRCIQCT RCIRFANDIA GIEEIGAINR GEHMEVTSYL 190 200 210 220 230 240 EQTLDSEISG NMIDICPVGA LNSKPYAFKA RKWELKHTAS IGVHDAEGSN IRIDSRADEI 250 260 270 280 290 300 MRILPSVNEA INEAWISDKN RFCYDGLKYQ RLDHPYIRKN GKLVEVSWSE ALKTIMDKIK 310 320 330 340 350 360 SVKPEKIAAI AGSIVSVEAM FMLKILLQKL GSNNYTVNQF NYKIDTSERG NYLFNTTIVG 370 380 390 400 410 420 VEKADLCLLI GANIRQIAPI LNSRIGRRVR IGALKVVRIG IGHNQTYKIH DLGNDIKIIE 430 440 450 460 470 480 DLAVGTHEYT KAFKEAKYPM IIVGDGVYGR DDGYALLSLI HKVVDKYNMM RDDWQGFNIL 490 500 510 520 530 540 HNHASIVGGL DIGFNTAIKF EGVKLAYLLG ADAIPFDKLK SAFIIYQGHH GDVGAMSADV 550 560 570 580 590 600 ILPSAAYTEQ SGIYVNLEGR PQIAQKAVSP VGRAKEDIEI IKEIAGYLKI DIGMDNLQEV 610 620 630 640 650 660 RIRLAKEYNI FANIDKITAN RFTQFISIDK LSQEPIAARP INYYMTDVIS KNSVTMAKCV 670 EAKEERKRDV AKVFY

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed: 15317790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017197 Genomic DNA. Translation: AAU04240.1.
RefSeq	YP_067722.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2958618.
GenomeReviews	Gene locus RT0784 in contig AE017197_GR.
KEGG	rty:RT0784.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q68VV2.
OMA	Q68VV2. RMSSGVT.
Enzyme and pathway databases
BioCyc	RTYP257363:RT0784-MON.
BRENDA	1.6.99.5. 281221.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOG_RICTY

Accession

Primary (citable) accession number: Q68VV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)