Q68VU1 (MIAB_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-

Alternative name(s):
tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	RT0795

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01864.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864		PRO_0000374510
Regions
	Domain	3 – 124	122	MTTase N-terminal
	Domain	383 – 445	63	TRAM
Sites
	Metal binding	12	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	87	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	162	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	166	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	169	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68VU1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: EBB911D8098E7ED5	FASTA	445	50,651
	10 20 30 40 50 60 MSKKLYIKTY GCQMNVYDSV KIQDLLYPFG YESTEDIKEA DIIILNTCHI REKAAEKTYS 70 80 90 100 110 120 ELGRIKKLQN TRKQEGLNPA IIVVAGCVAQ AEGEVIFTRT PYVDIVVGPQ SYYNLPELIS 130 140 150 160 170 180 KVVRHEKHLI DLDFVEEAKF DNLPEQLYTQ GASSFISVQE GCDKFCTFCV VPYTRGAEFS 190 200 210 220 230 240 RSVEQVYRES LKVVSNDTKE IILLGQNVNA YHGKGPKDKI FTLADLLKHL AQIPNLERLR 250 260 270 280 290 300 YMTSHPIDMT DDLIKLHGTE PKLMPFLHLP VQSGSNKILK AMNRKHDRDY YFNIINRLRE 310 320 330 340 350 360 ARSDIVLSSD FIVGFPGETE KDFEDTLDLV QRVKYGQCYS FKYSPRPGTP GAIRTDQIPE 370 380 390 400 410 420 HIKSKRLTIL QKELATQQLA FNESCVGSTM KVLFDRDGKF EDQIIGKTPY MQSVYIHNPN 430 440 KSLLGKIVDV IITKAALNSL TGEIL

References

[1]

"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017197 Genomic DNA. Translation: AAU04251.1.
RefSeq	YP_067733.1. NC_006142.1.
3D structure databases
ProteinModelPortal	Q68VU1.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU04251; AAU04251; RT0795.
GeneID	2958595.
KEGG	rty:RT0795.
PATRIC	17910842. VBIRicTyp34752_0814.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224767.
KO	K06168.
OMA	GIDRIRY.
ProtClustDB	PRK14325.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-835-MONOMER.
Family and domain databases
Gene3D	3.80.30.20. 1 hit.
HAMAP	MF_01864. tRNA_metthiotr_MiaB.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. miaB-methiolase. 1 hit. TIGR00089. TIGR00089. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_RICTY

Accession

Primary (citable) accession number: Q68VU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program