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Q68VU1 (MIAB_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB

EC=2.-.-.-
Alternative name(s):
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:RT0795
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864
PRO_0000374510

Regions

Domain3 – 124122MTTase N-terminal
Domain383 – 44563TRAM

Sites

Metal binding121Iron-sulfur (4Fe-4S) By similarity
Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding871Iron-sulfur (4Fe-4S) By similarity
Metal binding1621Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1661Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1691Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68VU1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: EBB911D8098E7ED5

FASTA44550,651
        10         20         30         40         50         60 
MSKKLYIKTY GCQMNVYDSV KIQDLLYPFG YESTEDIKEA DIIILNTCHI REKAAEKTYS 

        70         80         90        100        110        120 
ELGRIKKLQN TRKQEGLNPA IIVVAGCVAQ AEGEVIFTRT PYVDIVVGPQ SYYNLPELIS 

       130        140        150        160        170        180 
KVVRHEKHLI DLDFVEEAKF DNLPEQLYTQ GASSFISVQE GCDKFCTFCV VPYTRGAEFS 

       190        200        210        220        230        240 
RSVEQVYRES LKVVSNDTKE IILLGQNVNA YHGKGPKDKI FTLADLLKHL AQIPNLERLR 

       250        260        270        280        290        300 
YMTSHPIDMT DDLIKLHGTE PKLMPFLHLP VQSGSNKILK AMNRKHDRDY YFNIINRLRE 

       310        320        330        340        350        360 
ARSDIVLSSD FIVGFPGETE KDFEDTLDLV QRVKYGQCYS FKYSPRPGTP GAIRTDQIPE 

       370        380        390        400        410        420 
HIKSKRLTIL QKELATQQLA FNESCVGSTM KVLFDRDGKF EDQIIGKTPY MQSVYIHNPN 

       430        440 
KSLLGKIVDV IITKAALNSL TGEIL 

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017197 Genomic DNA. Translation: AAU04251.1.
RefSeqYP_067733.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68VU1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING257363.RT0795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU04251; AAU04251; RT0795.
GeneID2958595.
KEGGrty:RT0795.
PATRIC17910842. VBIRicTyp34752_0814.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0621.
HOGENOMHOG000224767.
KOK06168.
OMAIDAYGRD.
OrthoDBEOG6P5ZD8.
ProtClustDBPRK14325.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-835-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_RICTY
AccessionPrimary (citable) accession number: Q68VU1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names