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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi87Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi162Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi166Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi169Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:RT0795
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003745101 – 445tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST445

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0795.

Structurei

3D structure databases

ProteinModelPortaliQ68VU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 124MTTase N-terminalUniRule annotationAdd BLAST122
Domaini383 – 445TRAMUniRule annotationAdd BLAST63

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIPMDLIL.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiView protein in InterPro
IPR006638. Elp3/MiaB/NifB.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
SFLDiSFLDF00273. (dimethylallyl)adenosine_tRNA_. 1 hit.
SFLDG01061. methylthiotransferase. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiView protein in PROSITE
PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q68VU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKLYIKTY GCQMNVYDSV KIQDLLYPFG YESTEDIKEA DIIILNTCHI
60 70 80 90 100
REKAAEKTYS ELGRIKKLQN TRKQEGLNPA IIVVAGCVAQ AEGEVIFTRT
110 120 130 140 150
PYVDIVVGPQ SYYNLPELIS KVVRHEKHLI DLDFVEEAKF DNLPEQLYTQ
160 170 180 190 200
GASSFISVQE GCDKFCTFCV VPYTRGAEFS RSVEQVYRES LKVVSNDTKE
210 220 230 240 250
IILLGQNVNA YHGKGPKDKI FTLADLLKHL AQIPNLERLR YMTSHPIDMT
260 270 280 290 300
DDLIKLHGTE PKLMPFLHLP VQSGSNKILK AMNRKHDRDY YFNIINRLRE
310 320 330 340 350
ARSDIVLSSD FIVGFPGETE KDFEDTLDLV QRVKYGQCYS FKYSPRPGTP
360 370 380 390 400
GAIRTDQIPE HIKSKRLTIL QKELATQQLA FNESCVGSTM KVLFDRDGKF
410 420 430 440
EDQIIGKTPY MQSVYIHNPN KSLLGKIVDV IITKAALNSL TGEIL
Length:445
Mass (Da):50,651
Last modified:October 11, 2004 - v1
Checksum:iEBB911D8098E7ED5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU04251.1.
RefSeqiWP_011191226.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04251; AAU04251; RT0795.
KEGGirty:RT0795.
PATRICi17910842. VBIRicTyp34752_0814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU04251.1.
RefSeqiWP_011191226.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ68VU1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0795.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU04251; AAU04251; RT0795.
KEGGirty:RT0795.
PATRICi17910842. VBIRicTyp34752_0814.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIPMDLIL.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiView protein in InterPro
IPR006638. Elp3/MiaB/NifB.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
SFLDiSFLDF00273. (dimethylallyl)adenosine_tRNA_. 1 hit.
SFLDG01061. methylthiotransferase. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiView protein in PROSITE
PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAB_RICTY
AccessioniPrimary (citable) accession number: Q68VU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 11, 2004
Last modified: March 15, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.