Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi87Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi162Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi166Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi169Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRTYP257363:G1G0L-801-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:RT0795
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003745101 – 445tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST445

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi257363.RT0795

Structurei

3D structure databases

ProteinModelPortaliQ68VU1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 124MTTase N-terminalUniRule annotationAdd BLAST122
Domaini383 – 445TRAMUniRule annotationAdd BLAST63

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW Bacteria
COG0621 LUCA
HOGENOMiHOG000224767
KOiK06168
OMAiKVCEHFH
OrthoDBiPOG091H00LG

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q68VU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKLYIKTY GCQMNVYDSV KIQDLLYPFG YESTEDIKEA DIIILNTCHI
60 70 80 90 100
REKAAEKTYS ELGRIKKLQN TRKQEGLNPA IIVVAGCVAQ AEGEVIFTRT
110 120 130 140 150
PYVDIVVGPQ SYYNLPELIS KVVRHEKHLI DLDFVEEAKF DNLPEQLYTQ
160 170 180 190 200
GASSFISVQE GCDKFCTFCV VPYTRGAEFS RSVEQVYRES LKVVSNDTKE
210 220 230 240 250
IILLGQNVNA YHGKGPKDKI FTLADLLKHL AQIPNLERLR YMTSHPIDMT
260 270 280 290 300
DDLIKLHGTE PKLMPFLHLP VQSGSNKILK AMNRKHDRDY YFNIINRLRE
310 320 330 340 350
ARSDIVLSSD FIVGFPGETE KDFEDTLDLV QRVKYGQCYS FKYSPRPGTP
360 370 380 390 400
GAIRTDQIPE HIKSKRLTIL QKELATQQLA FNESCVGSTM KVLFDRDGKF
410 420 430 440
EDQIIGKTPY MQSVYIHNPN KSLLGKIVDV IITKAALNSL TGEIL
Length:445
Mass (Da):50,651
Last modified:October 11, 2004 - v1
Checksum:iEBB911D8098E7ED5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA Translation: AAU04251.1
RefSeqiWP_011191226.1, NC_006142.1

Genome annotation databases

EnsemblBacteriaiAAU04251; AAU04251; RT0795
KEGGirty:RT0795

Similar proteinsi

Entry informationi

Entry nameiMIAB_RICTY
AccessioniPrimary (citable) accession number: Q68VU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health