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Q68VR6

- RFTRM_RICTY

UniProt

Q68VR6 - RFTRM_RICTY

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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631S-adenosyl-L-methionineBy similarity
Binding sitei192 – 1921S-adenosyl-L-methionineBy similarity
Binding sitei207 – 2071S-adenosyl-L-methionineBy similarity
Binding sitei347 – 3471S-adenosyl-L-methionineBy similarity
Binding sitei372 – 3721S-adenosyl-L-methionineBy similarity
Binding sitei399 – 3991S-adenosyl-L-methionineBy similarity
Active sitei421 – 4211By similarity
Binding sitei421 – 4211S-adenosyl-L-methionineBy similarity
Binding sitei425 – 4251SubstrateBy similarity
Binding sitei457 – 4571SubstrateBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRTYP257363:GJEQ-876-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RT0836
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
ProteomesiUP000000604: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Bifunctional methyltransferasePRO_0000229299Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi257363.RT0836.

Structurei

3D structure databases

ProteinModelPortaliQ68VR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 302302RF MTaseAdd
BLAST
Regioni1 – 300300HemKBy similarityAdd
BLAST
Regioni140 – 1445S-adenosyl-L-methionine bindingBy similarity
Regioni207 – 2104Substrate bindingBy similarity
Regioni301 – 518218tRNA (guanine-N(7)-)-methyltransferaseBy similarityAdd
BLAST
Regioni305 – 518214tRNA MTaseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68VR6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQYSIKQILN NANDKLNKIG INLPELEARI LLQHVTNKPI EHLLIKLNEQ
60 70 80 90 100
LSEAEIEAFE KLLERRLEHE PIAYITGIKE FYSREFIVNK HVLIPRIDTE
110 120 130 140 150
ILIDVVIGLV VSRNDLNTCS KLKSLDSVKT IQHYNILELG TGSGCIAISL
160 170 180 190 200
LCELPNTSVI ATDISVDAIK VAKSNTIKHN VTDRIQIIHS NWFEKLNKQK
210 220 230 240 250
FDLIVSNPPY ISHSEKLEMA IETINYEPHI ALFAEEDGLE AYSIIAKNAK
260 270 280 290 300
QFLKPNGKII LEIGFSQAEK VCQIFLNYGY NIDHIYQDLQ SHNRVIEISP
310 320 330 340 350
INLNRSYARR IGKSLSKMQQ KLLDNELPKY LFSKEKFASE KRKIFLEIGF
360 370 380 390 400
GMGEHFINQA KINPDTLFIG VEVYLNGVAN VLKHAAQHNI MNFLLFPNNL
410 420 430 440 450
DLILNDLPNN SLDGIYILFP DPWIKNKKKK KRILNKERLN ILQNKLKNNG
460 470 480 490 500
NLVFASDIEN YFYETITLIR QNGNFEIIHN DDYLKPHDNY IITKYHQKAI
510
NENRTAKFMI LQHALTGH
Length:518
Mass (Da):59,700
Last modified:October 11, 2004 - v1
Checksum:i0DE0F08E4E220130
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017197 Genomic DNA. Translation: AAU04290.1.
RefSeqiYP_067772.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04290; AAU04290; RT0836.
GeneIDi2958550.
KEGGirty:RT0836.
PATRICi17910924. VBIRicTyp34752_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017197 Genomic DNA. Translation: AAU04290.1 .
RefSeqi YP_067772.1. NC_006142.1.

3D structure databases

ProteinModelPortali Q68VR6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 257363.RT0836.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU04290 ; AAU04290 ; RT0836 .
GeneIDi 2958550.
KEGGi rty:RT0836.
PATRICi 17910924. VBIRicTyp34752_0853.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000010896.
KOi K02493.
OMAi QQNLLDN.
OrthoDBi EOG68Q0SZ.

Enzyme and pathway databases

BioCyci RTYP257363:GJEQ-876-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-144 / Wilmington.

Entry informationi

Entry nameiRFTRM_RICTY
AccessioniPrimary (citable) accession number: Q68VR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2004
Last modified: October 1, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3