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Q68VR6

- RFTRM_RICTY

UniProt

Q68VR6 - RFTRM_RICTY

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Protein
Bifunctional methyltransferase
Gene
prmC/trmB, hemK, RT0836
Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity.UniRule annotation
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631S-adenosyl-L-methionine By similarity
Binding sitei192 – 1921S-adenosyl-L-methionine By similarity
Binding sitei207 – 2071S-adenosyl-L-methionine By similarity
Binding sitei347 – 3471S-adenosyl-L-methionine By similarity
Binding sitei372 – 3721S-adenosyl-L-methionine By similarity
Binding sitei399 – 3991S-adenosyl-L-methionine By similarity
Active sitei421 – 4211 By similarity
Binding sitei421 – 4211S-adenosyl-L-methionine By similarity
Binding sitei425 – 4251Substrate By similarity
Binding sitei457 – 4571Substrate By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRTYP257363:GJEQ-876-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional methyltransferase
    Including the following 2 domains:
    Release factor glutamine methyltransferase (EC:2.1.1.297)
    Short name:
    RF MTase
    Alternative name(s):
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:prmC/trmB
    Synonyms:hemK
    Ordered Locus Names:RT0836
    OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
    Taxonomic identifieri257363 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
    ProteomesiUP000000604: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 518518Bifunctional methyltransferaseUniRule annotation
    PRO_0000229299Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi257363.RT0836.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68VR6.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 302302RF MTaseUniRule annotation
    Add
    BLAST
    Regioni1 – 300300HemK By similarity
    Add
    BLAST
    Regioni140 – 1445S-adenosyl-L-methionine binding By similarity
    Regioni207 – 2104Substrate binding By similarity
    Regioni301 – 518218tRNA (guanine-N(7)-)-methyltransferase By similarity
    Add
    BLAST
    Regioni305 – 518214tRNA MTaseUniRule annotation
    Add
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
    In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000010896.
    KOiK02493.
    OMAiQQNLLDN.
    OrthoDBiEOG68Q0SZ.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68VR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQYSIKQILN NANDKLNKIG INLPELEARI LLQHVTNKPI EHLLIKLNEQ    50
    LSEAEIEAFE KLLERRLEHE PIAYITGIKE FYSREFIVNK HVLIPRIDTE 100
    ILIDVVIGLV VSRNDLNTCS KLKSLDSVKT IQHYNILELG TGSGCIAISL 150
    LCELPNTSVI ATDISVDAIK VAKSNTIKHN VTDRIQIIHS NWFEKLNKQK 200
    FDLIVSNPPY ISHSEKLEMA IETINYEPHI ALFAEEDGLE AYSIIAKNAK 250
    QFLKPNGKII LEIGFSQAEK VCQIFLNYGY NIDHIYQDLQ SHNRVIEISP 300
    INLNRSYARR IGKSLSKMQQ KLLDNELPKY LFSKEKFASE KRKIFLEIGF 350
    GMGEHFINQA KINPDTLFIG VEVYLNGVAN VLKHAAQHNI MNFLLFPNNL 400
    DLILNDLPNN SLDGIYILFP DPWIKNKKKK KRILNKERLN ILQNKLKNNG 450
    NLVFASDIEN YFYETITLIR QNGNFEIIHN DDYLKPHDNY IITKYHQKAI 500
    NENRTAKFMI LQHALTGH 518
    Length:518
    Mass (Da):59,700
    Last modified:October 11, 2004 - v1
    Checksum:i0DE0F08E4E220130
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU04290.1.
    RefSeqiYP_067772.1. NC_006142.1.

    Genome annotation databases

    EnsemblBacteriaiAAU04290; AAU04290; RT0836.
    GeneIDi2958550.
    KEGGirty:RT0836.
    PATRICi17910924. VBIRicTyp34752_0853.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017197 Genomic DNA. Translation: AAU04290.1 .
    RefSeqi YP_067772.1. NC_006142.1.

    3D structure databases

    ProteinModelPortali Q68VR6.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 257363.RT0836.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU04290 ; AAU04290 ; RT0836 .
    GeneIDi 2958550.
    KEGGi rty:RT0836.
    PATRICi 17910924. VBIRicTyp34752_0853.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000010896.
    KOi K02493.
    OMAi QQNLLDN.
    OrthoDBi EOG68Q0SZ.

    Enzyme and pathway databases

    BioCyci RTYP257363:GJEQ-876-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-144 / Wilmington.

    Entry informationi

    Entry nameiRFTRM_RICTY
    AccessioniPrimary (citable) accession number: Q68VR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: October 11, 2004
    Last modified: June 11, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia typhi
      Rickettsia typhi (strain Wilmington): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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