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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia typhi (strain ATCC VR-144 / Wilmington)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163S-adenosyl-L-methionineBy similarity1
Binding sitei192S-adenosyl-L-methionineBy similarity1
Binding sitei207S-adenosyl-L-methionineBy similarity1
Binding sitei347S-adenosyl-L-methionineBy similarity1
Binding sitei372S-adenosyl-L-methionineBy similarity1
Binding sitei399S-adenosyl-L-methionineBy similarity1
Active sitei421By similarity1
Binding sitei421S-adenosyl-L-methionineBy similarity1
Binding sitei425SubstrateBy similarity1
Binding sitei457SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RT0836
OrganismiRickettsia typhi (strain ATCC VR-144 / Wilmington)
Taxonomic identifieri257363 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000000604 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002292991 – 518Bifunctional methyltransferaseAdd BLAST518

Interactioni

Protein-protein interaction databases

STRINGi257363.RT0836.

Structurei

3D structure databases

ProteinModelPortaliQ68VR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 302RF MTaseAdd BLAST302
Regioni1 – 300HemKBy similarityAdd BLAST300
Regioni140 – 144S-adenosyl-L-methionine bindingBy similarity5
Regioni207 – 210Substrate bindingBy similarity4
Regioni301 – 518tRNA (guanine-N(7)-)-methyltransferaseBy similarityAdd BLAST218
Regioni305 – 518tRNA MTaseAdd BLAST214

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG0220. LUCA.
COG2890. LUCA.
HOGENOMiHOG000010896.
KOiK02493.
OMAiDDISENC.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
MF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68VR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYSIKQILN NANDKLNKIG INLPELEARI LLQHVTNKPI EHLLIKLNEQ
60 70 80 90 100
LSEAEIEAFE KLLERRLEHE PIAYITGIKE FYSREFIVNK HVLIPRIDTE
110 120 130 140 150
ILIDVVIGLV VSRNDLNTCS KLKSLDSVKT IQHYNILELG TGSGCIAISL
160 170 180 190 200
LCELPNTSVI ATDISVDAIK VAKSNTIKHN VTDRIQIIHS NWFEKLNKQK
210 220 230 240 250
FDLIVSNPPY ISHSEKLEMA IETINYEPHI ALFAEEDGLE AYSIIAKNAK
260 270 280 290 300
QFLKPNGKII LEIGFSQAEK VCQIFLNYGY NIDHIYQDLQ SHNRVIEISP
310 320 330 340 350
INLNRSYARR IGKSLSKMQQ KLLDNELPKY LFSKEKFASE KRKIFLEIGF
360 370 380 390 400
GMGEHFINQA KINPDTLFIG VEVYLNGVAN VLKHAAQHNI MNFLLFPNNL
410 420 430 440 450
DLILNDLPNN SLDGIYILFP DPWIKNKKKK KRILNKERLN ILQNKLKNNG
460 470 480 490 500
NLVFASDIEN YFYETITLIR QNGNFEIIHN DDYLKPHDNY IITKYHQKAI
510
NENRTAKFMI LQHALTGH
Length:518
Mass (Da):59,700
Last modified:October 11, 2004 - v1
Checksum:i0DE0F08E4E220130
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU04290.1.
RefSeqiWP_011191264.1. NC_006142.1.

Genome annotation databases

EnsemblBacteriaiAAU04290; AAU04290; RT0836.
KEGGirty:RT0836.
PATRICi17910924. VBIRicTyp34752_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017197 Genomic DNA. Translation: AAU04290.1.
RefSeqiWP_011191264.1. NC_006142.1.

3D structure databases

ProteinModelPortaliQ68VR6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257363.RT0836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU04290; AAU04290; RT0836.
KEGGirty:RT0836.
PATRICi17910924. VBIRicTyp34752_0853.

Phylogenomic databases

eggNOGiENOG4105EQY. Bacteria.
COG0220. LUCA.
COG2890. LUCA.
HOGENOMiHOG000010896.
KOiK02493.
OMAiDDISENC.
OrthoDBiPOG091H02CJ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_02126. RF_methyltr_PrmC. 1 hit.
MF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFTRM_RICTY
AccessioniPrimary (citable) accession number: Q68VR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia typhi
    Rickettsia typhi (strain Wilmington): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.