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Q68VR6 (RFTRM_RICTY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional methyltransferase

Including the following 2 domains:

  1. Release factor glutamine methyltransferase
    Short name=RF MTase
    EC=2.1.1.n16
    Alternative name(s):
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
  2. tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
Gene names
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RT0836
OrganismRickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]
Taxonomic identifier257363 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group ›

Protein attributes

Sequence length518 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity. HAMAP-Rule MF_01057

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + glutamine in release factor = S-adenosyl-L-homocysteine + N5-methylglutamine in release factor. HAMAP-Rule MF_01057

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Sequence similarities

In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 518518Bifunctional methyltransferase HAMAP-Rule MF_01057
PRO_0000229299

Regions

Region1 – 302302RF MTase HAMAP-Rule MF_01057
Region1 – 300300HemK By similarity
Region140 – 1445S-adenosyl-L-methionine binding By similarity
Region207 – 2104Substrate binding By similarity
Region301 – 518218tRNA (guanine-N(7)-)-methyltransferase By similarity
Region305 – 518214tRNA MTase HAMAP-Rule MF_01057

Sites

Binding site1631S-adenosyl-L-methionine By similarity
Binding site1921S-adenosyl-L-methionine By similarity
Binding site2071S-adenosyl-L-methionine By similarity
Binding site3471S-adenosyl-L-methionine By similarity
Binding site3721S-adenosyl-L-methionine By similarity
Binding site3991S-adenosyl-L-methionine By similarity
Binding site4211S-adenosyl-L-methionine By similarity
Binding site4251Substrate By similarity
Binding site4571Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q68VR6 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 0DE0F08E4E220130

FASTA51859,700
        10         20         30         40         50         60 
MQYSIKQILN NANDKLNKIG INLPELEARI LLQHVTNKPI EHLLIKLNEQ LSEAEIEAFE 

        70         80         90        100        110        120 
KLLERRLEHE PIAYITGIKE FYSREFIVNK HVLIPRIDTE ILIDVVIGLV VSRNDLNTCS 

       130        140        150        160        170        180 
KLKSLDSVKT IQHYNILELG TGSGCIAISL LCELPNTSVI ATDISVDAIK VAKSNTIKHN 

       190        200        210        220        230        240 
VTDRIQIIHS NWFEKLNKQK FDLIVSNPPY ISHSEKLEMA IETINYEPHI ALFAEEDGLE 

       250        260        270        280        290        300 
AYSIIAKNAK QFLKPNGKII LEIGFSQAEK VCQIFLNYGY NIDHIYQDLQ SHNRVIEISP 

       310        320        330        340        350        360 
INLNRSYARR IGKSLSKMQQ KLLDNELPKY LFSKEKFASE KRKIFLEIGF GMGEHFINQA 

       370        380        390        400        410        420 
KINPDTLFIG VEVYLNGVAN VLKHAAQHNI MNFLLFPNNL DLILNDLPNN SLDGIYILFP 

       430        440        450        460        470        480 
DPWIKNKKKK KRILNKERLN ILQNKLKNNG NLVFASDIEN YFYETITLIR QNGNFEIIHN 

       490        500        510 
DDYLKPHDNY IITKYHQKAI NENRTAKFMI LQHALTGH

« Hide

References

[1]"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae."
McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. expand/collapse author list , Hong C., Yu X.-J., Walker D.H., Weinstock G.M.
J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017197 Genomic DNA. Translation: AAU04290.1.
RefSeqYP_067772.1. NC_006142.1.

3D structure databases

ProteinModelPortalQ68VR6.
ModBaseSearch...

Protein-protein interaction databases

STRING257363.RT0836.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU04290; AAU04290; RT0836.
GeneID2958550.
KEGGrty:RT0836.
PATRIC17910924. VBIRicTyp34752_0853.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000010896.
KOK02493.
OMAIGVEVYL.
ProtClustDBPRK01544.

Enzyme and pathway databases

BioCycRTYP257363:GJEQ-876-MONOMER.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB. Fused.
MF_02126. RF_methyltr_PrmC. Fused.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRFTRM_RICTY
AccessionPrimary (citable) accession number: Q68VR6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 11, 2004
Last modified: May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia typhi

Rickettsia typhi (strain Wilmington): entries and gene names

SIMILARITY comments

Index of protein domains and families

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