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Protein

Double-stranded RNA-binding protein Staufen homolog 2

Gene

Stau2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein required for the microtubule-dependent transport of neuronal RNA from the cell body to the dendrite. As protein synthesis occurs within the dendrite, the localization of specific mRNAs to dendrites may be a prerequisite for neurite outgrowth and plasticity at sites distant from the cell body.1 Publication

GO - Molecular functioni

  • Hsp70 protein binding Source: RGD
  • kinesin binding Source: RGD
  • mitogen-activated protein kinase binding Source: RGD
  • ribosome binding Source: RGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • anterograde dendritic transport of messenger ribonucleoprotein complex Source: SynGO
  • cellular response to oxidative stress Source: RGD
  • eye morphogenesis Source: RGD
  • positive regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of long term synaptic depression Source: RGD
  • positive regulation of synapse assembly Source: RGD
  • regulation of actin cytoskeleton organization Source: RGD
  • regulation of filopodium assembly Source: RGD
  • regulation of gene expression Source: RGD

Keywordsi

Molecular functionRNA-binding
Biological processTransport

Names & Taxonomyi

Protein namesi
Recommended name:
Double-stranded RNA-binding protein Staufen homolog 2
Short name:
r-staufen protein
Gene namesi
Name:Stau2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621479. Stau2.

Subcellular locationi

  • Nucleusnucleolus
  • Nucleus
  • Cytoplasm
  • Endoplasmic reticulum

  • Note: Shuttles between the nucleolus, nucleus and the cytoplasm. Nuclear export of isoform 1 is independent of XPO1/CRM1 and may require the exportin XPO5. Nuclear export of isoform 2 and isoform 3 can occur by both XPO1/CRM1-dependent and XPO1/CRM1-independent pathways. Found in large cytoplasmic ribonucleoprotein (RNP) granules which are present in the actin rich regions of myelinating processes and associated with microtubules, polysomes and the endoplasmic reticulum. Also recruited to stress granules (SGs) upon inhibition of translation or oxidative stress. These structures are thought to harbor housekeeping mRNAs when translation is aborted.

GO - Cellular componenti

  • axon Source: RGD
  • cytoplasm Source: RGD
  • cytoplasmic stress granule Source: RGD
  • dendrite Source: RGD
  • dendrite cytoplasm Source: GOC
  • dendritic shaft Source: RGD
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • messenger ribonucleoprotein complex Source: RGD
  • microtubule Source: UniProtKB-KW
  • neuronal cell body Source: RGD
  • nuclear membrane Source: RGD
  • nucleolus Source: UniProtKB-SubCell
  • nucleus Source: RGD

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi239F → A: Abrogates CRM1-independent nuclear export. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000722481 – 571Double-stranded RNA-binding protein Staufen homolog 2Add BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei426PhosphoserineBy similarity1
Modified residuei440PhosphoserineBy similarity1
Modified residuei456PhosphoserineBy similarity1
Modified residuei493PhosphoserineBy similarity1
Isoform 2 (identifier: Q68SB1-2)
Modified residuei9PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei18PhosphothreonineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Isoform 3 (identifier: Q68SB1-3)
Modified residuei9PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei18PhosphothreonineCombined sources1
Modified residuei21PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ68SB1.
PRIDEiQ68SB1.

PTM databases

PhosphoSitePlusiQ68SB1.

Expressioni

Tissue specificityi

Expressed in both somata and dendrites of hippocampal neurons.2 Publications

Interactioni

Subunit structurei

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with the exportin XPO5. This requires RNA and RAN bound to GTP (By similarity). Interacts with microtubules. Isoform 2 and isoform 3 may also interact with ribosomes, and this association is independent of translation.By similarity2 Publications

GO - Molecular functioni

  • Hsp70 protein binding Source: RGD
  • kinesin binding Source: RGD
  • mitogen-activated protein kinase binding Source: RGD

Protein-protein interaction databases

BioGridi251277. 5 interactors.
STRINGi10116.ENSRNOP00000060092.

Structurei

3D structure databases

ProteinModelPortaliQ68SB1.
SMRiQ68SB1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 75DRBM 1PROSITE-ProRule annotationAdd BLAST68
Domaini95 – 181DRBM 2PROSITE-ProRule annotationAdd BLAST87
Domaini207 – 274DRBM 3PROSITE-ProRule annotationAdd BLAST68
Domaini307 – 375DRBM 4PROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni381 – 571Required for dendritic transportAdd BLAST191

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi273 – 317Nuclear localization signal 1Add BLAST45
Motifi373 – 412Nuclear localization signal 2Add BLAST40

Domaini

The DRBM 3 domain appears to be the major RNA-binding determinant (By similarity). This domain also mediates interaction with XPO5 and is required for XPO1/CRM1-independent nuclear export.By similarity1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3732. Eukaryota.
ENOG410XSCK. LUCA.
HOVERGENiHBG005013.
InParanoidiQ68SB1.
KOiK17597.
PhylomeDBiQ68SB1.

Family and domain databases

InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR032478. Staufen_C.
PfamiView protein in Pfam
PF00035. dsrm. 4 hits.
PF16482. Staufen_C. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 4 hits.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 4 hits.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68SB1-1) [UniParc]FASTAAdd to basket
Also known as: LL, A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANPKEKTPM CLVNELARFH SIQPQYKLLN ESGPAHSKMF SVQLSLGEQT
60 70 80 90 100
WESEGSSIKK AQQAVANKAL TESTLPKPVQ KPPKSNVNNN PGSITPTVEL
110 120 130 140 150
NGLAMKRGEP AIYRPLDPKP FPNYRANYNF RGMYNQRYHC PMPKIFYVQL
160 170 180 190 200
TVGNNEFFGE GKTRQAARHN AAMKALQALQ NEPIPEKSPQ NGESGKEMDD
210 220 230 240 250
DKDANKSEIS LVFEIALKRN MPVSFEVIKE SGPPHMKSFV TRVSVGEFSA
260 270 280 290 300
EGEGNSKKLS KKRAATTVLQ ELKKLPPLPV IEKPKLFFKK RPKTIIKAGP
310 320 330 340 350
EYGQGMNPIS RLAQIQQARK EKEPDYVLLS ERGMPRRREF VMQVKVGNEV
360 370 380 390 400
ATGTGPNKKI AKKNAAEAML LQLGYKASTS LQDQLDKTGE NKGWSGPKPG
410 420 430 440 450
FPEPANNTPK GILHLSPDVY QEMEASRHRV TSGTTLGYLS PKDMNQPSSS
460 470 480 490 500
FFSVESPSPT SSAPAARELL MNGTSPAAEA IGLKGSSPTP PCSSVQPSKQ
510 520 530 540 550
LEYLARIQGF QAALSALKQF SEQGLESIDG VVNVENGSLE KQAKHLREKA
560 570
DNNQANPGSI TQDCKKSKSV I
Length:571
Mass (Da):62,681
Last modified:October 11, 2004 - v1
Checksum:i778DDC940C66075D
GO
Isoform 2 (identifier: Q68SB1-2) [UniParc]FASTAAdd to basket
Also known as: SL

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     33-38: GPAHSK → MLQINQ

Note: XPO1/CRM1-dependent nuclear export is mediated by residues 4-14 and is abrogated by mutagenesis of Ile-4 or Leu-12.Combined sources
Show »
Length:539
Mass (Da):59,118
Checksum:iC51A66716A6E0FCF
GO
Isoform 3 (identifier: Q68SB1-3) [UniParc]FASTAAdd to basket
Also known as: SS

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.
     33-38: GPAHSK → MLQINQ
     512-512: A → V
     513-571: Missing.

Note: XPO1/CRM1-dependent nuclear export is mediated by residues 4-14 and is abrogated by mutagenesis of Ile-4 or Leu-12.Combined sources
Show »
Length:480
Mass (Da):52,810
Checksum:i49F6AC357B0D9E0E
GO
Isoform 4 (identifier: Q68SB1-4) [UniParc]FASTAAdd to basket
Also known as: LS, B

The sequence of this isoform differs from the canonical sequence as follows:
     512-512: A → V
     513-571: Missing.

Show »
Length:512
Mass (Da):56,373
Checksum:i031E18D9659DC119
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti339E → K in AAL89718 (PubMed:11709157).Curated1
Sequence conflicti339E → K in AAL89719 (PubMed:11709157).Curated1
Sequence conflicti538S → F in AAL89718 (PubMed:11709157).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153851 – 32Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST32
Alternative sequenceiVSP_01538633 – 38GPAHSK → MLQINQ in isoform 2 and isoform 3. 2 Publications6
Alternative sequenceiVSP_015387512A → V in isoform 3 and isoform 4. 4 Publications1
Alternative sequenceiVSP_015388513 – 571Missing in isoform 3 and isoform 4. 4 PublicationsAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF483620 mRNA. Translation: AAL89718.1.
AF483621 mRNA. Translation: AAL89719.1.
AY684789 mRNA. Translation: AAU21478.1.
AY684790 mRNA. Translation: AAU21479.1.
AY549445 mRNA. Translation: AAT36670.1.
AY549446 mRNA. Translation: AAT36671.1.
AY549447 mRNA. Translation: AAT36672.1.
AY549448 mRNA. Translation: AAT36673.1.
BC085705 mRNA. Translation: AAH85705.1.
RefSeqiNP_001007150.1. NM_001007149.1.
NP_001007151.1. NM_001007150.1. [Q68SB1-3]
NP_604461.2. NM_134466.2. [Q68SB1-4]
UniGeneiRn.73714.

Genome annotation databases

GeneIDi171500.
KEGGirno:171500.
UCSCiRGD:621479. rat. [Q68SB1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF483620 mRNA. Translation: AAL89718.1.
AF483621 mRNA. Translation: AAL89719.1.
AY684789 mRNA. Translation: AAU21478.1.
AY684790 mRNA. Translation: AAU21479.1.
AY549445 mRNA. Translation: AAT36670.1.
AY549446 mRNA. Translation: AAT36671.1.
AY549447 mRNA. Translation: AAT36672.1.
AY549448 mRNA. Translation: AAT36673.1.
BC085705 mRNA. Translation: AAH85705.1.
RefSeqiNP_001007150.1. NM_001007149.1.
NP_001007151.1. NM_001007150.1. [Q68SB1-3]
NP_604461.2. NM_134466.2. [Q68SB1-4]
UniGeneiRn.73714.

3D structure databases

ProteinModelPortaliQ68SB1.
SMRiQ68SB1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251277. 5 interactors.
STRINGi10116.ENSRNOP00000060092.

PTM databases

PhosphoSitePlusiQ68SB1.

Proteomic databases

PaxDbiQ68SB1.
PRIDEiQ68SB1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171500.
KEGGirno:171500.
UCSCiRGD:621479. rat. [Q68SB1-1]

Organism-specific databases

CTDi27067.
RGDi621479. Stau2.

Phylogenomic databases

eggNOGiKOG3732. Eukaryota.
ENOG410XSCK. LUCA.
HOVERGENiHBG005013.
InParanoidiQ68SB1.
KOiK17597.
PhylomeDBiQ68SB1.

Miscellaneous databases

PROiPR:Q68SB1.

Family and domain databases

InterProiView protein in InterPro
IPR014720. dsRBD_dom.
IPR032478. Staufen_C.
PfamiView protein in Pfam
PF00035. dsrm. 4 hits.
PF16482. Staufen_C. 1 hit.
SMARTiView protein in SMART
SM00358. DSRM. 4 hits.
PROSITEiView protein in PROSITE
PS50137. DS_RBD. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSTAU2_RAT
AccessioniPrimary (citable) accession number: Q68SB1
Secondary accession number(s): Q68SB2
, Q68SB3, Q68SB4, Q8R4C9, Q8R4D0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 11, 2004
Last modified: May 10, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.