Q68RJ8 (Q68RJ8_LEIMA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Trypanothione-dependent glyoxalase I EMBL AAT98624.1
EC=4.4.1.- EMBL AAT98624.1

Gene names

Name:	GLO1 EMBL AAT98624.1
ORF Names:	LMJF_35_3010 EMBL CBZ12929.1

Organism

Leishmania major EMBL AAT98624.1

Taxonomic identifier

5664 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Metal-binding PDB 2C21 Nickel PDB 2C21
Molecular function	Lyase EMBL AAT98624.1
Technical term	3D-structure PDB 2C21 Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	8	1	Nickel; via tele nitrogen PDB 2C21
	Metal binding	59	1	Nickel PDB 2C21
	Metal binding	77	1	Nickel; via tele nitrogen PDB 2C21
	Metal binding	120	1	Nickel PDB 2C21

Sequences

	Sequence		Length	Mass (Da)
	Q68RJ8 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: AFB4549A3B2C5CE5	FASTA	141	16,319
	10 20 30 40 50 60 MPSRRMLHTM IRVGDLDRSI KFYTERLGMK VLRKWDVPED KYTLVFLGYG PEMSSTVLEL 70 80 90 100 110 120 TYNYGVTSYK HDEAYGHIAI GVEDVKELVA DMRKHDVPID YEDESGFMAF VVDPDGYYIE 130 140 LLNEKTMMEK AEADMKEQGT A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A trypanothione-dependent glyoxalase I with a prokaryotic ancestry in Leishmania major." Vickers T.J., Greig N., Fairlamb A.H. Proc. Natl. Acad. Sci. U.S.A. 101:13186-13191(2004) [PubMed: 15329410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The genome of the kinetoplastid parasite, Leishmania major." Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J. Science 309:436-442(2005) [PubMed: 16020728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Friedlin EMBL CBZ12929.1 and MHOM/IL/81/Friedlin.
[3]	"Specificity of the trypanothione-dependent Leishmania major glyoxalase I: structure and biochemical comparison with the human enzyme." Ariza A., Vickers T.J., Greig N., Armour K.A., Dixon M.J., Eggleston I.M., Fairlamb A.H., Bond C.S. Mol. Microbiol. 59:1239-1248(2006) [PubMed: 16430697] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH NICKEL.
[4]	Aslett M. Submitted (JAN-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Friedlin EMBL CBZ12929.1.
[5]	"Chromosome and gene copy number variation allow genomic structural differences between species and strains of Leishmania." Hilley J.D., Rogers M., Wilkes J., Dickens N.J., Bates P., Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D., Saad W., Seeger K., Berriman M., Smith D.F., Hertz-Fowler C., Mottram J.C. Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Friedlin EMBL CBZ12929.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY604654 Genomic DNA. Translation: AAT98624.1.
FR796431 Genomic DNA. Translation: CBZ12929.1.

RefSeq

XP_843406.1. XM_838313.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2C21	X-ray	2.00	A/B/C/D/E/F	1-141	[»]

ProteinModelPortal

Q68RJ8.

SMR

Q68RJ8. Positions 3-141.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblProtists

LmjF.35.3010:mRNA; LmjF.35.3010:pep; LmjF.35.3010.

GeneID

3684468.

GenomeReviews

Gene locus GLO1 in contig FR796431_GR.

KEGG

lma:LmjF35.3010.

Organism-specific databases

EuPathDB

EupathDB:LmjF.35.3010.

Phylogenomic databases

OMA

TYNWDTN.

PhylomeDB

Q68RJ8.

ProtClustDB

CLSZ2429531.

Enzyme and pathway databases

BRENDA

4.4.1.5. 19061.

Family and domain databases

InterPro

IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]

KO

K01759.

TIGRFAMs

TIGR00068. Glyox_I. 1 hit.

PROSITE

PS00934. GLYOXALASE_I_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q68RJ8_LEIMA

Accession

Primary (citable) accession number: Q68RJ8
Secondary accession number(s): E9AFC5, Q4FWG9

Entry history

Integrated into UniProtKB/TrEMBL:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)