Q68J44 (DUPD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity phosphatase DUPD1
EC=3.1.3.16
EC=3.1.3.48

Alternative name(s):
Dual specificity phosphatase 27

Gene names

Name:	DUPD1
Synonyms:	DUSP27

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Homodimer. Ref.3
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Skeletal muscle, liver and adipose tissue. Ref.1
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 220

220

Dual specificity phosphatase DUPD1

PRO_0000295877

Regions

Domain

54 – 201

148

Tyrosine-protein phosphatase

Region

146 – 153

8

Substrate binding Probable

Sites

Active site

147

1

Phosphocysteine intermediate By similarity

Natural variations

Natural variant

66

1

D → N.
Corresponds to variant rs11594934 [ dbSNP | Ensembl ].

VAR_051757

Natural variant

137

1

S → R.
Corresponds to variant rs16931938 [ dbSNP | Ensembl ].

VAR_033376

Secondary structure

1

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220

Helix Strand Turn

Details...

Helix

38 – 47

10

Beta strand

54 – 59

6

Beta strand

62 – 65

4

Helix

67 – 71

5

Helix

73 – 78

6

Beta strand

83 – 86

4

Helix

97 – 100

4

Turn

101 – 103

3

Beta strand

107 – 110

4

Helix

121 – 124

4

Helix

125 – 136

12

Beta strand

143 – 146

4

Beta strand

148 – 152

5

Helix

153 – 165

13

Helix

170 – 178

9

Helix

187 – 205

19

Sequences

	Sequence		Length	Mass (Da)
	Q68J44 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A4DA1E37FD39D5A3	FASTA	220	25,336
	10 20 30 40 50 60 MTSGEVKTSL KNAYSSAKRL SPKMEEEGEE EDYCTPGAFE LERLFWKGSP QYTHVNEVWP 70 80 90 100 110 120 KLYIGDEATA LDRYRLQKAG FTHVLNAAHG RWNVDTGPDY YRDMDIQYHG VEADDLPTFD 130 140 150 160 170 180 LSVFFYPAAA FIDRALSDDH SKILVHCVMG RSRSATLVLA YLMIHKDMTL VDAIQQVAKN 190 200 210 220 RCVLPNRGFL KQLRELDKQL VQQRRRSQRQ DGEEEDGREL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of DUSP27, a novel dual-specific protein phosphatase." Friedberg I., Nika K., Tautz L., Saito K., Cerignoli F., Friedberg I., Godzik A., Mustelin T. FEBS Lett. 581:2527-2533(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Structure of human dual-specificity phosphatase 27 at 2.38 A resolution." Lountos G.T., Tropea J.E., Waugh D.S. Acta Crystallogr. D 67:471-479(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) IN COMPLEX WITH SULFATE, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY686755 mRNA. Translation: AAT94288.1.
BC137321 mRNA. Translation: AAI37322.1.
BC137322 mRNA. Translation: AAI37323.1.

IPI

IPI00456156.

RefSeq

NP_001003892.1. NM_001003892.1.

UniGene

Hs.535090.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Y96	X-ray	2.38	A/B	2-220	[»]

ProteinModelPortal

Q68J44.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000340609.

PTM databases

PhosphoSite

Q68J44.

Polymorphism databases

DMDM

74748317.

Proteomic databases

PaxDb

Q68J44.

PRIDE

Q68J44.

Protocols and materials databases

DNASU

338599.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000338487; ENSP00000340609; ENSG00000188716.

GeneID

338599.

KEGG

hsa:338599.

UCSC

uc001jwq.1. human.

Organism-specific databases

CTD

338599.

GeneCards

GC10M076797.

HGNC

HGNC:23481. DUPD1.

HPA

HPA042964.

neXtProt

NX_Q68J44.

PharmGKB

PA134959776.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2453.

HOGENOM

HOG000233767.

HOVERGEN

HBG001524.

InParanoid

Q68J44.

KO

K14165.

OMA

MTVVDAI.

OrthoDB

EOG4R23W1.

Gene expression databases

Bgee

Q68J44.

CleanEx

HS_DUPD1.
HS_DUSP27.

Genevestigator

Q68J44.

Family and domain databases

InterPro

IPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]

PANTHER

PTHR10159. PTHR10159. 1 hit.

Pfam

PF00782. DSPc. 1 hit.
[Graphical view]

PRINTS

PR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.

SMART

SM00195. DSPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

338599.

NextBio

97059.

Entry information

Entry name

DUPD1_HUMAN

Accession

Primary (citable) accession number: Q68J44
Secondary accession number(s): B2RP93

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.