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Q68G12 (SIAT9_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lactosylceramide alpha-2,3-sialyltransferase

EC=2.4.99.9
Alternative name(s):
CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase
Ganglioside GM3 synthase
ST3Gal V
Short name=ST3GalV
Sialyltransferase 9
Gene names
Name:St3gal5
Synonyms:Siat9
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D-glucosylceramide) By similarity.

Catalytic activity

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Sequence caution

The sequence BAA33492.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Lactosylceramide alpha-2,3-sialyltransferase
PRO_0000334618

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421Helical; Signal-anchor for type II membrane protein; Potential
Topological domain55 – 387333Extracellular Potential

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Disulfide bond167 ↔ 325 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q68G12 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: BF53342B7E8C06EA

FASTA38744,640
        10         20         30         40         50         60 
MPNEFTSAKL RSDCSRTSLQ WYTQTQHKMR RPSLLLKDIL KCMLVVFGVW LLYILKLNYT 

        70         80         90        100        110        120 
AEECDMKKLN YVDPARIKRA HRNTQEVFQK ECRPGHAKKT MDLLFKGKYS MDLEPFVQKI 

       130        140        150        160        170        180 
PTASEAELKY DPPFGFRKFS SKVQSLLDML PEHDFPEHLR AKHCKRCVVI GNGGILHGLE 

       190        200        210        220        230        240 
LGHALNQFDV VIRLNSAPIE GYSEHVGNKT TIRMTYPEGA PLSDAEYYAN DLFVAVLFKS 

       250        260        270        280        290        300 
VDFKWLQAMV KNESLPFWIR LFFWKQVAEK IPLQPKHFRI LNPVIIKETA FDILQYSEPQ 

       310        320        330        340        350        360 
SRFWGHDKNI PTIGIIAIVL ATHLCDEVSL AGFGYDLSQP RTPLHYFDSQ CMGAMNWQVM 

       370        380 
HNVTTETQFL QKLIKEGVVQ DLSGGIH 

« Hide

References

« Hide 'large scale' references
[1]"Rat GM3 synthase cDNA."
Ishii A., Saito M.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB018049 mRNA. Translation: BAA33492.1. Different initiation.
BC078798 mRNA. Translation: AAH78798.1.
RefSeqNP_112627.2. NM_031337.2.
XP_006236739.1. XM_006236677.1.
XP_006236740.1. XM_006236678.1.
XP_006236741.1. XM_006236679.1.
UniGeneRn.22706.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

Proteomic databases

PRIDEQ68G12.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000050249; ENSRNOP00000042426; ENSRNOG00000010284.
GeneID83505.
KEGGrno:83505.
UCSCRGD:620875. rat.

Organism-specific databases

CTD8869.
RGD620875. St3gal5.

Phylogenomic databases

eggNOGNOG257001.
GeneTreeENSGT00620000087815.
HOGENOMHOG000000682.
HOVERGENHBG056676.
InParanoidQ68G12.
KOK03370.
OMAFFWKQVA.
OrthoDBEOG76X609.
PhylomeDBQ68G12.

Gene expression databases

ArrayExpressQ68G12.
GenevestigatorQ68G12.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Other

NextBio615932.
PROQ68G12.

Entry information

Entry nameSIAT9_RAT
AccessionPrimary (citable) accession number: Q68G12
Secondary accession number(s): O88830
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families