Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lactosylceramide alpha-2,3-sialyltransferase

Gene

St3gal5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D-glucosylceramide).By similarity

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

GO - Molecular functioni

  1. lactosylceramide alpha-2,3-sialyltransferase activity Source: UniProtKB

GO - Biological processi

  1. protein glycosylation Source: InterPro
  2. sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_213723. Sialic acid metabolism.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide alpha-2,3-sialyltransferase (EC:2.4.99.9)
Alternative name(s):
CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase
Ganglioside GM3 synthase
ST3Gal V
Short name:
ST3GalV
Sialyltransferase 9
Gene namesi
Name:St3gal5
Synonyms:Siat9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi620875. St3gal5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei34 – 5421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini55 – 387333ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Lactosylceramide alpha-2,3-sialyltransferasePRO_0000334618Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi167 ↔ 325By similarity
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ68G12.

Expressioni

Gene expression databases

GenevestigatoriQ68G12.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257001.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000000682.
HOVERGENiHBG056676.
InParanoidiQ68G12.
KOiK03370.
OMAiFFWKQVA.
OrthoDBiEOG76X609.
PhylomeDBiQ68G12.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q68G12-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPNEFTSAKL RSDCSRTSLQ WYTQTQHKMR RPSLLLKDIL KCMLVVFGVW
60 70 80 90 100
LLYILKLNYT AEECDMKKLN YVDPARIKRA HRNTQEVFQK ECRPGHAKKT
110 120 130 140 150
MDLLFKGKYS MDLEPFVQKI PTASEAELKY DPPFGFRKFS SKVQSLLDML
160 170 180 190 200
PEHDFPEHLR AKHCKRCVVI GNGGILHGLE LGHALNQFDV VIRLNSAPIE
210 220 230 240 250
GYSEHVGNKT TIRMTYPEGA PLSDAEYYAN DLFVAVLFKS VDFKWLQAMV
260 270 280 290 300
KNESLPFWIR LFFWKQVAEK IPLQPKHFRI LNPVIIKETA FDILQYSEPQ
310 320 330 340 350
SRFWGHDKNI PTIGIIAIVL ATHLCDEVSL AGFGYDLSQP RTPLHYFDSQ
360 370 380
CMGAMNWQVM HNVTTETQFL QKLIKEGVVQ DLSGGIH
Length:387
Mass (Da):44,640
Last modified:October 11, 2004 - v1
Checksum:iBF53342B7E8C06EA
GO

Sequence cautioni

The sequence BAA33492.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018049 mRNA. Translation: BAA33492.1. Different initiation.
BC078798 mRNA. Translation: AAH78798.1.
RefSeqiNP_112627.2. NM_031337.2.
XP_006236739.1. XM_006236677.1.
XP_006236741.1. XM_006236679.2.
XP_008761218.1. XM_008762996.1.
UniGeneiRn.22706.

Genome annotation databases

EnsembliENSRNOT00000050249; ENSRNOP00000042426; ENSRNOG00000010284.
GeneIDi83505.
KEGGirno:83505.
UCSCiRGD:620875. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018049 mRNA. Translation: BAA33492.1. Different initiation.
BC078798 mRNA. Translation: AAH78798.1.
RefSeqiNP_112627.2. NM_031337.2.
XP_006236739.1. XM_006236677.1.
XP_006236741.1. XM_006236679.2.
XP_008761218.1. XM_008762996.1.
UniGeneiRn.22706.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Proteomic databases

PRIDEiQ68G12.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050249; ENSRNOP00000042426; ENSRNOG00000010284.
GeneIDi83505.
KEGGirno:83505.
UCSCiRGD:620875. rat.

Organism-specific databases

CTDi8869.
RGDi620875. St3gal5.

Phylogenomic databases

eggNOGiNOG257001.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000000682.
HOVERGENiHBG056676.
InParanoidiQ68G12.
KOiK03370.
OMAiFFWKQVA.
OrthoDBiEOG76X609.
PhylomeDBiQ68G12.

Enzyme and pathway databases

ReactomeiREACT_213723. Sialic acid metabolism.

Miscellaneous databases

NextBioi615932.
PROiQ68G12.

Gene expression databases

GenevestigatoriQ68G12.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat GM3 synthase cDNA."
    Ishii A., Saito M.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiSIAT9_RAT
AccessioniPrimary (citable) accession number: Q68G12
Secondary accession number(s): O88830
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 11, 2004
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.