Reviewed, UniProtKB/Swiss-Prot Q68FX1 (MPI_RAT)

Names and origin

Protein names

Recommended name:
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
Alternative name(s):
    Phosphomannose isomerase
      Short name=PMI
    Phosphohexomutase

Gene names

Name:

Mpi

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
PTM	Acetylation
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Mannose-6-phosphate isomerase		PRO_0000194238
Sites
	Active site	295	1	By similarity
	Metal binding	110	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	137	1	Zinc By similarity
	Metal binding	276	1	Zinc By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FX1-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 69C6CE365535002B	FASTA	423	46,424
	10 20 30 40 50 60 MANPRVFPLS CVVQQYAWGK VGSKSEVACL LACSDPLTQI SEDKPYAELW MGAHPRGDAK 70 80 90 100 110 120 ILDNRISQKT LGQWIAENQN SLGQKVKDTF NGKLPFLFKV LSVETALSIQ AHPNKELAEK 130 140 150 160 170 180 LHLQAPEHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKKVPEFQSL IGEDATAQLK 190 200 210 220 230 240 KSMNEGSGAM ASALKNCFSH LMKSEKKVVV EQLNLLVKRI SQQISNGNSM DDICGELLLQ 250 260 270 280 290 300 LHQQYPGDIG CFAIYLLNLI TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP 310 320 330 340 350 360 KFIDVSTLCE MLDYTPSPSK DRLFAPTLSQ DDPYLSIYDP PVPDFTVMKI EVPGSVTEYK 370 380 390 400 410 420 VLTLDSASIL LLVQGTVTAI IPSVQGEIPL SRGGVLFIGA NETVLLKLTV PKNLLIFRAC CLL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079111 mRNA. Translation: AAH79111.1.
IPI	IPI00470302.
RefSeq	NP_001004081.1.
UniGene	Rn.44246
3D structure databases
SMR	Q68FX1. Positions 4-422.
ModBase	Search...
Protein-protein interaction databases
STRING	Q68FX1.
Genome annotation databases
Ensembl	ENSRNOT00000025783; ENSRNOP00000025783; ENSRNOG00000018898; Rattus norvegicus. [Genome view]
GeneID	300741.
KEGG	rno:300741.
NMPDR	fig|10116.3.peg.28690.
UCSC	NM_001004081. rat.
Organism-specific databases
CTD	300741.
RGD	3107. Mpi.
Phylogenomic databases
eggNOG	roNOG08296.
HOVERGEN	Q68FX1.
InParanoid	Q68FX1.
OMA	DFTVMKM.
OrthoDB	EOG96X1VJ.
PhylomeDB	Q68FX1.
Enzyme and pathway databases
BRENDA	5.3.1.8. 248.
Gene expression databases
ArrayExpress	Q68FX1.
Genevestigator	Q68FX1.
GermOnline	ENSRNOG00000018898. Rattus norvegicus.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	647412.

Entry information

Entry name

MPI_RAT

Accession

Primary (citable) accession number: Q68FX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)