Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mannose-6-phosphate isomerase

Gene

Mpi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathway:iGDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase (Mpi)
  2. Phosphomannomutase (Pmm1), Phosphomannomutase (Pmm2)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101ZincBy similarity
Metal bindingi112 – 1121ZincBy similarity
Metal bindingi137 – 1371ZincBy similarity
Metal bindingi276 – 2761ZincBy similarity
Active sitei295 – 2951By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_274980. Synthesis of GDP-mannose.
SABIO-RKQ68FX1.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Name:Mpi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi3107. Mpi.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 423422Mannose-6-phosphate isomerasePRO_0000194238Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei102 – 1021PhosphoserineBy similarity
Modified residuei108 – 1081PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ68FX1.
PRIDEiQ68FX1.

PTM databases

PhosphoSiteiQ68FX1.

Expressioni

Gene expression databases

GenevisibleiQ68FX1. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025783.

Structurei

3D structure databases

ProteinModelPortaliQ68FX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
GeneTreeiENSGT00390000016075.
HOGENOMiHOG000241277.
HOVERGENiHBG000367.
InParanoidiQ68FX1.
KOiK01809.
OMAiQGEPWDT.
OrthoDBiEOG7GTT3M.
PhylomeDBiQ68FX1.
TreeFamiTF312831.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANPRVFPLS CVVQQYAWGK VGSKSEVACL LACSDPLTQI SEDKPYAELW
60 70 80 90 100
MGAHPRGDAK ILDNRISQKT LGQWIAENQN SLGQKVKDTF NGKLPFLFKV
110 120 130 140 150
LSVETALSIQ AHPNKELAEK LHLQAPEHYP DANHKPEMAI ALTPFQGLCG
160 170 180 190 200
FRPVEEIVTF LKKVPEFQSL IGEDATAQLK KSMNEGSGAM ASALKNCFSH
210 220 230 240 250
LMKSEKKVVV EQLNLLVKRI SQQISNGNSM DDICGELLLQ LHQQYPGDIG
260 270 280 290 300
CFAIYLLNLI TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP
310 320 330 340 350
KFIDVSTLCE MLDYTPSPSK DRLFAPTLSQ DDPYLSIYDP PVPDFTVMKI
360 370 380 390 400
EVPGSVTEYK VLTLDSASIL LLVQGTVTAI IPSVQGEIPL SRGGVLFIGA
410 420
NETVLLKLTV PKNLLIFRAC CLL
Length:423
Mass (Da):46,424
Last modified:October 11, 2004 - v1
Checksum:i69C6CE365535002B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079111 mRNA. Translation: AAH79111.1.
RefSeqiNP_001004081.1. NM_001004081.1.
UniGeneiRn.229358.

Genome annotation databases

EnsembliENSRNOT00000025783; ENSRNOP00000025783; ENSRNOG00000018898.
GeneIDi300741.
KEGGirno:300741.
UCSCiRGD:3107. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079111 mRNA. Translation: AAH79111.1.
RefSeqiNP_001004081.1. NM_001004081.1.
UniGeneiRn.229358.

3D structure databases

ProteinModelPortaliQ68FX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025783.

PTM databases

PhosphoSiteiQ68FX1.

Proteomic databases

PaxDbiQ68FX1.
PRIDEiQ68FX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025783; ENSRNOP00000025783; ENSRNOG00000018898.
GeneIDi300741.
KEGGirno:300741.
UCSCiRGD:3107. rat.

Organism-specific databases

CTDi4351.
RGDi3107. Mpi.

Phylogenomic databases

eggNOGiCOG1482.
GeneTreeiENSGT00390000016075.
HOGENOMiHOG000241277.
HOVERGENiHBG000367.
InParanoidiQ68FX1.
KOiK01809.
OMAiQGEPWDT.
OrthoDBiEOG7GTT3M.
PhylomeDBiQ68FX1.
TreeFamiTF312831.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
ReactomeiREACT_274980. Synthesis of GDP-mannose.
SABIO-RKQ68FX1.

Miscellaneous databases

NextBioi647412.
PROiQ68FX1.

Gene expression databases

GenevisibleiQ68FX1. RN.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiMPI_RAT
AccessioniPrimary (citable) accession number: Q68FX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 11, 2004
Last modified: July 22, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.