Q68FX1 (MPI_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannose-6-phosphate isomerase
EC=5.3.1.8

Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI

Gene names

Name:

Mpi

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Mannose-6-phosphate isomerase		PRO_0000194238
Sites
	Active site	295	1	By similarity
	Metal binding	110	1	Zinc By similarity
	Metal binding	112	1	Zinc By similarity
	Metal binding	137	1	Zinc By similarity
	Metal binding	276	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FX1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 69C6CE365535002B	FASTA	423	46,424
	10 20 30 40 50 60 MANPRVFPLS CVVQQYAWGK VGSKSEVACL LACSDPLTQI SEDKPYAELW MGAHPRGDAK 70 80 90 100 110 120 ILDNRISQKT LGQWIAENQN SLGQKVKDTF NGKLPFLFKV LSVETALSIQ AHPNKELAEK 130 140 150 160 170 180 LHLQAPEHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKKVPEFQSL IGEDATAQLK 190 200 210 220 230 240 KSMNEGSGAM ASALKNCFSH LMKSEKKVVV EQLNLLVKRI SQQISNGNSM DDICGELLLQ 250 260 270 280 290 300 LHQQYPGDIG CFAIYLLNLI TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP 310 320 330 340 350 360 KFIDVSTLCE MLDYTPSPSK DRLFAPTLSQ DDPYLSIYDP PVPDFTVMKI EVPGSVTEYK 370 380 390 400 410 420 VLTLDSASIL LLVQGTVTAI IPSVQGEIPL SRGGVLFIGA NETVLLKLTV PKNLLIFRAC CLL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079111 mRNA. Translation: AAH79111.1.
IPI	IPI00470302.
RefSeq	NP_001004081.1. NM_001004081.1.
UniGene	Rn.229358.
3D structure databases
ProteinModelPortal	Q68FX1.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000025783.
PTM databases
PhosphoSite	Q68FX1.
Proteomic databases
PaxDb	Q68FX1.
PRIDE	Q68FX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025783; ENSRNOP00000025783; ENSRNOG00000018898.
GeneID	300741.
KEGG	rno:300741.
UCSC	RGD:3107. rat.
Organism-specific databases
CTD	4351.
RGD	3107. Mpi.
Phylogenomic databases
eggNOG	COG1482.
GeneTree	ENSGT00390000016075.
HOGENOM	HOG000241277.
HOVERGEN	HBG000367.
InParanoid	Q68FX1.
KO	K01809.
OMA	VMKMEVP.
OrthoDB	EOG49CQ7M.
Enzyme and pathway databases
SABIO-RK	Q68FX1.
UniPathway	UPA00126; UER00423.
Gene expression databases
Genevestigator	Q68FX1.
GermOnline	ENSRNOG00000018898. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.120.10. 3 hits.
InterPro	IPR001250. Man6P_Isoase-1. IPR016305. Mannose-6-P_Isomerase. IPR018050. Pmannose_isomerase-type1_CS. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR10309. PTHR10309. 1 hit.
Pfam	PF01238. PMI_typeI. 1 hit. [Graphical view]
PIRSF	PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTS	PR00714. MAN6PISMRASE.
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR00218. manA. 1 hit.
PROSITE	PS00965. PMI_I_1. 1 hit. PS00966. PMI_I_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	647412.

Entry information

Entry name

MPI_RAT

Accession

Primary (citable) accession number: Q68FX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	October 11, 2004
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program