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Protein

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene

Idh3B

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei133 – 1331SubstrateBy similarity
Binding sitei164 – 1641SubstrateBy similarity
Sitei171 – 1711Critical for catalysisBy similarity
Sitei218 – 2181Critical for catalysisBy similarity
Metal bindingi251 – 2511Magnesium or manganeseBy similarity
Binding sitei251 – 2511SubstrateBy similarity

GO - Molecular functioni

  • isocitrate dehydrogenase (NAD+) activity Source: RGD
  • magnesium ion binding Source: InterPro
  • NAD binding Source: InterPro

GO - Biological processi

  • 2-oxoglutarate metabolic process Source: RGD
  • isocitrate metabolic process Source: RGD
  • NADH metabolic process Source: RGD
  • tricarboxylic acid cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

ReactomeiREACT_338796. Citric acid cycle (TCA cycle).
SABIO-RKQ68FX0.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit beta
NAD(+)-specific ICDH subunit beta
Gene namesi
Name:Idh3B
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621881. Idh3B.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial isocitrate dehydrogenase complex (NAD+) Source: RGD
  • nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionBy similarityAdd
BLAST
Chaini35 – 385351Isocitrate dehydrogenase [NAD] subunit beta, mitochondrialPRO_0000014447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ68FX0.
PRIDEiQ68FX0.

2D gel databases

World-2DPAGE0004:Q68FX0.

PTM databases

PhosphoSiteiQ68FX0.

Expressioni

Gene expression databases

ExpressionAtlasiQ68FX0. baseline and differential.
GenevisibleiQ68FX0. RN.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

BioGridi250169. 1 interaction.
STRINGi10116.ENSRNOP00000009681.

Structurei

3D structure databases

ProteinModelPortaliQ68FX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ68FX0.
KOiK00030.
OMAiHTEGELQ.
OrthoDBiEOG75B85R.
PhylomeDBiQ68FX0.
TreeFamiTF315033.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALSNVRWL TRAVLGARNS GAWRGLRTAA SAQAASQSQA QDVRVEGAFP
60 70 80 90 100
VTMLPGDGVG PELMHAVKEV FKAAAVPVEF KEHHLSEVQN MASEEKLEQV
110 120 130 140 150
LSSMKENKVA IIGKIYTPME YKGELASYDM QLRRKLDLFA NVVHVKSLPG
160 170 180 190 200
YKTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RTKSQRIAKF
210 220 230 240 250
AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFETMII
260 270 280 290 300
DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY
310 320 330 340 350
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK
360 370 380
KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPHGG
Length:385
Mass (Da):42,354
Last modified:October 11, 2004 - v1
Checksum:iDC91921B48EEED1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079113 mRNA. Translation: AAH79113.1.
RefSeqiNP_446033.1. NM_053581.1.
UniGeneiRn.1093.

Genome annotation databases

EnsembliENSRNOT00000009681; ENSRNOP00000009681; ENSRNOG00000007316.
GeneIDi94173.
KEGGirno:94173.
UCSCiRGD:621881. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079113 mRNA. Translation: AAH79113.1.
RefSeqiNP_446033.1. NM_053581.1.
UniGeneiRn.1093.

3D structure databases

ProteinModelPortaliQ68FX0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250169. 1 interaction.
STRINGi10116.ENSRNOP00000009681.

PTM databases

PhosphoSiteiQ68FX0.

2D gel databases

World-2DPAGE0004:Q68FX0.

Proteomic databases

PaxDbiQ68FX0.
PRIDEiQ68FX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009681; ENSRNOP00000009681; ENSRNOG00000007316.
GeneIDi94173.
KEGGirno:94173.
UCSCiRGD:621881. rat.

Organism-specific databases

CTDi3420.
RGDi621881. Idh3B.

Phylogenomic databases

eggNOGiCOG0473.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ68FX0.
KOiK00030.
OMAiHTEGELQ.
OrthoDBiEOG75B85R.
PhylomeDBiQ68FX0.
TreeFamiTF315033.

Enzyme and pathway databases

ReactomeiREACT_338796. Citric acid cycle (TCA cycle).
SABIO-RKQ68FX0.

Miscellaneous databases

NextBioi617826.
PROiQ68FX0.

Gene expression databases

ExpressionAtlasiQ68FX0. baseline and differential.
GenevisibleiQ68FX0. RN.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiIDH3B_RAT
AccessioniPrimary (citable) accession number: Q68FX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: July 22, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.