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Protein

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene

Idh3B

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.By similarity

Enzyme regulationi

The heterotetramer and the heterodimer composed of IDH3A and IDH3G subunits can be allosterically activated by citrate (CIT) or/and ADP, and the two activators can act independently or synergistically. The heterodimer composed of IDH3A and IDH3B subunits cannot be allosterically regulated and the allosteric regulation of the heterotetramer is through the IDH3G subunit and not the IDH3B subunit. The IDH3G subunit contains the allosteric site which consists of a CIT-binding site and an ADP-binding site, and the binding of CIT and ADP causes conformational changes at the allosteric site which are transmitted to the active site in the catalytic subunit (IDH3A) through a cascade of conformational changes at the heterodimer interface, leading to stabilization of the isocitrate-binding at the active site and thus activation of the enzyme. ATP can activate the heterotetramer and the heterodimer composed of IDH3A and IDH3G subunits at low concentrations but inhibits their activities at high concentrations, whereas ATP exhibits only inhibitory effect on the heterodimer composed of IDH3A and IDH3B subunits.By similarity

GO - Molecular functioni

  • isocitrate dehydrogenase (NAD+) activity Source: RGD
  • magnesium ion binding Source: InterPro
  • NAD binding Source: InterPro

GO - Biological processi

  • 2-oxoglutarate metabolic process Source: RGD
  • isocitrate metabolic process Source: RGD
  • NADH metabolic process Source: RGD
  • tricarboxylic acid cycle Source: RGD

Keywordsi

Biological processTricarboxylic acid cycle

Enzyme and pathway databases

ReactomeiR-RNO-71403. Citric acid cycle (TCA cycle).
SABIO-RKiQ68FX0.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
Alternative name(s):
Isocitric dehydrogenase subunit beta
NAD(+)-specific ICDH subunit beta
Gene namesi
Name:Idh3B
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621881. Idh3B.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial isocitrate dehydrogenase complex (NAD+) Source: RGD

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34MitochondrionBy similarityAdd BLAST34
ChainiPRO_000001444735 – 385Isocitrate dehydrogenase [NAD] subunit beta, mitochondrialAdd BLAST351

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ68FX0.
PRIDEiQ68FX0.

2D gel databases

World-2DPAGEi0004:Q68FX0.

PTM databases

iPTMnetiQ68FX0.
PhosphoSitePlusiQ68FX0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007316.
GenevisibleiQ68FX0. RN.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.By similarity

Protein-protein interaction databases

BioGridi250169. 2 interactors.
STRINGi10116.ENSRNOP00000009681.

Structurei

3D structure databases

ProteinModelPortaliQ68FX0.
SMRiQ68FX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0784. Eukaryota.
COG0473. LUCA.
GeneTreeiENSGT00590000083091.
HOGENOMiHOG000021113.
HOVERGENiHBG052080.
InParanoidiQ68FX0.
KOiK00030.
OMAiIVPGCNM.
OrthoDBiEOG091G094X.
PhylomeDBiQ68FX0.
TreeFamiTF315033.

Family and domain databases

InterProiView protein in InterPro
IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
PfamiView protein in Pfam
PF00180. Iso_dh. 1 hit.
SMARTiView protein in SMART
SM01329. Iso_dh. 1 hit.
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiView protein in PROSITE
PS00470. IDH_IMDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALSNVRWL TRAVLGARNS GAWRGLRTAA SAQAASQSQA QDVRVEGAFP
60 70 80 90 100
VTMLPGDGVG PELMHAVKEV FKAAAVPVEF KEHHLSEVQN MASEEKLEQV
110 120 130 140 150
LSSMKENKVA IIGKIYTPME YKGELASYDM QLRRKLDLFA NVVHVKSLPG
160 170 180 190 200
YKTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RTKSQRIAKF
210 220 230 240 250
AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFETMII
260 270 280 290 300
DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY
310 320 330 340 350
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK
360 370 380
KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPHGG
Length:385
Mass (Da):42,354
Last modified:October 11, 2004 - v1
Checksum:iDC91921B48EEED1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079113 mRNA. Translation: AAH79113.1.
RefSeqiNP_446033.1. NM_053581.1.
UniGeneiRn.1093.

Genome annotation databases

EnsembliENSRNOT00000009681; ENSRNOP00000009681; ENSRNOG00000007316.
GeneIDi94173.
KEGGirno:94173.
UCSCiRGD:621881. rat.

Similar proteinsi

Entry informationi

Entry nameiIDH3B_RAT
AccessioniPrimary (citable) accession number: Q68FX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: October 11, 2004
Last modified: August 30, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families