Q68FX0 (IDH3B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
EC=1.1.1.41

Alternative name(s):
Isocitric dehydrogenase subunit beta
NAD(+)-specific ICDH subunit beta

Gene names

Name:

Idh3B

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	2-oxoglutarate metabolic process Inferred from direct assay. Source: RGD NADH metabolic process Inferred from direct assay. Source: RGD isocitrate metabolic process Inferred from direct assay. Source: RGD tricarboxylic acid cycle Inferred from direct assay. Source: RGD
Cellular component	mitochondrial isocitrate dehydrogenase complex (NAD+) Inferred by curator. Source: RGD
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from direct assay. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 385	351	Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial		PRO_0000014447
Sites
	Metal binding	251	1	Magnesium or manganese By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Site	171	1	Critical for catalysis By similarity
	Site	218	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	146	1	N6-acetyllysine By similarity
	Modified residue	199	1	N6-acetyllysine By similarity
	Modified residue	374	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FX0 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: DC91921B48EEED1E	FASTA	385	42,354
	10 20 30 40 50 60 MAALSNVRWL TRAVLGARNS GAWRGLRTAA SAQAASQSQA QDVRVEGAFP VTMLPGDGVG 70 80 90 100 110 120 PELMHAVKEV FKAAAVPVEF KEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIYTPME 130 140 150 160 170 180 YKGELASYDM QLRRKLDLFA NVVHVKSLPG YKTRHNNLDL VIIREQTEGE YSSLEHESAR 190 200 210 220 230 240 GVIECLKIVT RTKSQRIAKF AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY 250 260 270 280 290 300 PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 310 320 330 340 350 360 AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK KVIKVGKVRT 370 380 RDMGGYSTTT DFIKSVIGHL HPHGG

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079113 mRNA. Translation: AAH79113.1.
IPI	IPI00357924.
RefSeq	NP_446033.1. NM_053581.1.
UniGene	Rn.1093.
3D structure databases
ProteinModelPortal	Q68FX0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q68FX0.
PTM databases
PhosphoSite	Q68FX0.
2D gel databases
World-2DPAGE	0004:Q68FX0.
Proteomic databases
PRIDE	Q68FX0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000009681; ENSRNOP00000009681; ENSRNOG00000007316.
GeneID	94173.
KEGG	rno:94173.
NMPDR	fig|10116.3.peg.19717.
UCSC	NM_053581. rat.
Organism-specific databases
CTD	3420.
RGD	621881. Idh3B.
Phylogenomic databases
eggNOG	roNOG15039.
HOVERGEN	HBG052080.
InParanoid	Q68FX0.
OMA	NPGAWRG.
OrthoDB	EOG43BMP4.
PhylomeDB	Q68FX0.
Gene expression databases
ArrayExpress	Q68FX0.
Genevestigator	Q68FX0.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KO	K00030.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF5. PTHR11835:SF5. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. Mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617826.

Entry information

Entry name

IDH3B_RAT

Accession

Primary (citable) accession number: Q68FX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program