Reviewed, UniProtKB/Swiss-Prot Q68FX0 (IDH3B_RAT)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

Idh3B

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: InterPro
Molecular function	isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 385	351	Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial		PRO_0000014447
Sites
	Metal binding	251	1	Magnesium or manganese By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Site	171	1	Critical for catalysis By similarity
	Site	218	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FX0-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: DC91921B48EEED1E	FASTA	385	42,354
	10 20 30 40 50 60 MAALSNVRWL TRAVLGARNS GAWRGLRTAA SAQAASQSQA QDVRVEGAFP VTMLPGDGVG 70 80 90 100 110 120 PELMHAVKEV FKAAAVPVEF KEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIYTPME 130 140 150 160 170 180 YKGELASYDM QLRRKLDLFA NVVHVKSLPG YKTRHNNLDL VIIREQTEGE YSSLEHESAR 190 200 210 220 230 240 GVIECLKIVT RTKSQRIAKF AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY 250 260 270 280 290 300 PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 310 320 330 340 350 360 AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK KVIKVGKVRT 370 380 RDMGGYSTTT DFIKSVIGHL HPHGG

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC079113 mRNA. Translation: AAH79113.1.
RefSeq	NP_446033.1.
UniGene	Rn.1093
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000007316. Rattus norvegicus. [Contig view]
GeneID	94173.
KEGG	rno:94173.
NMPDR	fig|10116.3.peg.19717.
Organism-specific databases
RGD	621881. Idh3B.
Phylogenomic databases
HOVERGEN	Q68FX0.
Gene expression databases
ArrayExpress	Q68FX0.
Family and domain databases
InterPro	IPR004434. IsoCit_DHase_NAD_mit. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	617826.

Entry information

Entry name

IDH3B_RAT

Accession

Primary (citable) accession number: Q68FX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 11, 2004
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)