Reviewed, UniProtKB/Swiss-Prot Q68FW7 (SYTM_RAT)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase, mitochondrial
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS
    Threonyl-tRNA synthetase-like 1

Gene names

Name:	Tars2
Synonyms:	Tarsl1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 723		Threonyl-tRNA synthetase, mitochondrial		PRO_0000254588

Sequences

	Sequence		Length	Mass (Da)
	Q68FW7-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 55D41FF833FCE14E	FASTA	723	81,672
	10 20 30 40 50 60 MGLCLRWRRL GFPLPGFRRC ELHTVREAPI PTPPHWLAER FGLFEELWTA QVKRLASMTQ 70 80 90 100 110 120 KKARTIKISL PEGQKVDAVA WNTTPYQLAQ QISSTLADTA VAAEVNGELY DLDRPLETDC 130 140 150 160 170 180 HLRFLTFDSP EGKAVFWRSS AHVLGAAAEQ HLGAVLCRGP STESGFYLDF FLGKERTVRS 190 200 210 220 230 240 TELPTLERIC QEIITAAQPF RRLEASRGQL RQLFKDNHFK LHVIEEKVTG TTATVYGCGM 250 260 270 280 290 300 SVDLCQGPHL RHTGQIGALK LLTNSSALWR SSEAPETLQR VSGISFPKAE LLRNWEARRE 310 320 330 340 350 360 EAELRDHRRI GKEQELFFFH ELSPGSCFFL PRGTRIYNAL VAFIRAEYAR RGFSEVKTPT 370 380 390 400 410 420 LFSTKLWEQS GHWEHYRAHM FSLKPPGTDG VDSSQSGHPA RCPKDTLALK PMNCPAHCLM 430 440 450 460 470 480 FAHRPRSWRE LPVRLADFGV LHRAEASGSL GGLTRLWRFQ QDDAHIFCAP SQLEAEIRGC 490 500 510 520 530 540 LDFLRSVYSV LGFSFHLALS TRPPGFLGEP HLWDQAEKVL QQALEEFGEP WNLNPGDGAF 550 560 570 580 590 600 YGPKIDVHLH DALGRPHQCG TIQLDFQLPL RFDLQYKGPA GAPECPVLIH RAVLGSVERL 610 620 630 640 650 660 LGVLAESCGG RWPLWLSPFQ VVVIPVRTEQ EDYARQVQQC LQAAGLVSDL DADCGLTLSR 670 680 690 700 710 720 RVRRAQLAHY NFQFVVGQRE QSQMSVNVRT RDNRQLGERG LAESVQRLLE LQDARVPNAE ELF

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079154 mRNA. Translation: AAH79154.1.
IPI	IPI00471567.
RefSeq	NP_001014062.1.
UniGene	Rn.44110
3D structure databases
HSSP	HSSP built from PDB template 1WWT based on UniProtKB P26639.
SMR	Q68FW7. Positions 305-714.
ModBase	Search...
Protein-protein interaction databases
STRING	Q68FW7.
Genome annotation databases
Ensembl	ENSRNOT00000044500; ENSRNOP00000044548; ENSRNOG00000027726; Rattus norvegicus. [Genome view]
GeneID	310672.
KEGG	rno:310672.
Organism-specific databases
CTD	310672.
RGD	1308283. Tars2.
Phylogenomic databases
eggNOG	roNOG08297.
HOVERGEN	Q68FW7.
PhylomeDB	Q68FW7.
Enzyme and pathway databases
BRENDA	6.1.1.3. 248.
Gene expression databases
Genevestigator	Q68FW7.
GermOnline	ENSRNOG00000027726. Rattus norvegicus.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	662404.

Entry information

Entry name

SYTM_RAT

Accession

Primary (citable) accession number: Q68FW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)