Q68FW2 (CDCA3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division cycle-associated protein 3

Alternative name(s):
Trigger of mitotic entry protein 1
Short name=TOME-1

Gene names

Name:	Cdca3
Synonyms:	Tome1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Domain	The KEN box is required for the association with the APC/C-Cdh1 complex By similarity.
Post-translational modification	Ubiquitinated and degraded by the APC/C-Cdh1 complex By similarity.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Cell division cycle-associated protein 3		PRO_0000287710
Regions
	Region	90 – 124	35	F-box-like
	Motif	263 – 265	3	KEN box
Amino acid modifications
	Modified residue	204	1	Phosphoserine By similarity
	Modified residue	207	1	Phosphothreonine By similarity
	Modified residue	214	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FW2 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 2EB02AE5CFDFBDE1	FASTA	273	29,611
	10 20 30 40 50 60 MGSTQSVSGT PARPLPRNKH VSRVADPRSP SAGIQRTPIQ VESSPQPNPP AEQLNSLKQA 70 80 90 100 110 120 QDPDPRSPTL GIARTPMKIS GPDSQCPLVK ELSEVFETEV SETEVSESIS SPVLGLPQET 130 140 150 160 170 180 PLSSELDLPP DPDPQVSLED QLLPWSQTEL NSKQVFAKEE AKQSTETMVS GQTSDKPSRD 190 200 210 220 230 240 PETPQSSGSK RSRRKTNNKV LGRSPLTILQ DDNSPGTLTL RQGKRPSALS ENVKDLKEGV 250 260 270 ILGTGRFLKA GGGAWEQNED HDKENQHFAL MES

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079204 mRNA. Translation: AAH79204.1.
IPI	IPI00364571.
RefSeq	NP_001007649.1. NM_001007648.1.
UniGene	Rn.129078.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000020803.
PTM databases
PhosphoSite	Q68FW2.
Proteomic databases
PaxDb	Q68FW2.
PRIDE	Q68FW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000020803; ENSRNOP00000020803; ENSRNOG00000015529.
GeneID	297594.
KEGG	rno:297594.
UCSC	RGD:1359093. rat.
Organism-specific databases
CTD	83461.
RGD	1359093. Cdca3.
Phylogenomic databases
eggNOG	NOG46427.
GeneTree	ENSGT00390000017343.
HOGENOM	HOG000013100.
HOVERGEN	HBG053225.
InParanoid	Q68FW2.
OMA	KTGGRAW.
OrthoDB	EOG4H9XMC.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q68FW2.
Family and domain databases
ProtoNet	Search...
Other
NextBio	642465.

Entry information

Entry name

CDCA3_RAT

Accession

Primary (citable) accession number: Q68FW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	October 11, 2004
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program