Reviewed, UniProtKB/Swiss-Prot Q68FT5 (BHMT2_RAT)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 2
    EC=2.1.1.5

Gene names

Name:

Bhmt2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Betaine--homocysteine S-methyltransferase 2		PRO_0000273227
Regions
	Domain	11 – 305	295	Hcy-binding
Sites
	Metal binding	208	1	Zinc By similarity
	Metal binding	290	1	Zinc By similarity
	Metal binding	291	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FT5-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 403AAC188B979BFE	FASTA	363	39,929
	10 20 30 40 50 60 MAPAGGPRVK KVILERLDSG EVVVGDGGFL FTLEKRGFVK AGLWTPEAVV EYPSAVRQLH 70 80 90 100 110 120 TEFLRAGADV LQTFTFSAAE DRMESKWEAV NAAACDLAQE VADGGAALVA GGICQTSLYK 130 140 150 160 170 180 YHKDETRIKN IFRLQLGVFA RKNVDFLIAE YFEHVEEAVW AVEVLREVGA PVAVTMCIGP 190 200 210 220 230 240 EGDMHGVTPG ECAVRLSRAG ANIIGVNCRF GPWTSLQTMK LMKEGLRDAG LQAHLMVQCL 250 260 270 280 290 300 GFHTPDCGKG GFVDLPEYPF GLEPRVATRW DIQKYAREAY NLGVRYIGGC CGFEPYHIRA 310 320 330 340 350 360 IAEELAPERG FLPPASEKHG IWGSGLDMHT KPWIRARARR EYWETLLPAS GRPFCPSLSK PDA

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC079366 mRNA. Translation: AAH79366.1.
IPI	IPI00471540.
RefSeq	NP_001014278.2.
UniGene	Rn.8494
3D structure databases
SMR	Q68FT5. Positions 10-363.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000061708; ENSRNOP00000058419; ENSRNOG00000040120; Rattus norvegicus. [Genome view]
GeneID	365972.
KEGG	rno:365972.
Organism-specific databases
CTD	365972.
RGD	1359418. Bhmt2.
Phylogenomic databases
HOVERGEN	Q68FT5.
Enzyme and pathway databases
BRENDA	2.1.1.5. 248.
Gene expression databases
Genevestigator	Q68FT5.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	688443.

Entry information

Entry name

BHMT2_RAT

Accession

Primary (citable) accession number: Q68FT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2004
Last modified:	October 13, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)