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Protein

S-methylmethionine--homocysteine S-methyltransferase BHMT2

Gene

Bhmt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline (By similarity).By similarity

Catalytic activityi

S-methyl-L-methionine + L-homocysteine = 2 L-methionine.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (BhmT route).
Proteins known to be involved in this subpathway in this organism are:
  1. S-methylmethionine--homocysteine S-methyltransferase BHMT2 (Bhmt2), Betaine--homocysteine S-methyltransferase 1 (Bhmt), Betaine--homocysteine S-methyltransferase 1 (Bhmt), S-methylmethionine--homocysteine S-methyltransferase BHMT2 (Bhmt2)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (BhmT route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi208 – 2081ZincPROSITE-ProRule annotation
Metal bindingi290 – 2901ZincPROSITE-ProRule annotation
Metal bindingi291 – 2911ZincPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00051; UER00083.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methylmethionine--homocysteine S-methyltransferase BHMT2 (EC:2.1.1.10)
Short name:
SMM-hcy methyltransferase
Alternative name(s):
Betaine--homocysteine S-methyltransferase 2
Gene namesi
Name:Bhmt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1359418. Bhmt2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363S-methylmethionine--homocysteine S-methyltransferase BHMT2PRO_0000273227Add
BLAST

Proteomic databases

PaxDbiQ68FT5.
PRIDEiQ68FT5.

PTM databases

iPTMnetiQ68FT5.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058419.

Structurei

3D structure databases

ProteinModelPortaliQ68FT5.
SMRiQ68FT5. Positions 10-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 305295Hcy-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Hcy-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1579. Eukaryota.
COG0646. LUCA.
HOGENOMiHOG000231636.
HOVERGENiHBG080367.
InParanoidiQ68FT5.
KOiK00547.
PhylomeDBiQ68FT5.

Family and domain databases

Gene3Di3.20.20.330. 1 hit.
InterProiIPR017226. Betaine-hCys_S-MeTrfase_BHMT.
IPR003726. HCY_dom.
[Graphical view]
PfamiPF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF037505. Betaine_HMT. 1 hit.
SUPFAMiSSF82282. SSF82282. 1 hit.
PROSITEiPS50970. HCY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68FT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPAGGPRVK KVILERLDSG EVVVGDGGFL FTLEKRGFVK AGLWTPEAVV
60 70 80 90 100
EYPSAVRQLH TEFLRAGADV LQTFTFSAAE DRMESKWEAV NAAACDLAQE
110 120 130 140 150
VADGGAALVA GGICQTSLYK YHKDETRIKN IFRLQLGVFA RKNVDFLIAE
160 170 180 190 200
YFEHVEEAVW AVEVLREVGA PVAVTMCIGP EGDMHGVTPG ECAVRLSRAG
210 220 230 240 250
ANIIGVNCRF GPWTSLQTMK LMKEGLRDAG LQAHLMVQCL GFHTPDCGKG
260 270 280 290 300
GFVDLPEYPF GLEPRVATRW DIQKYAREAY NLGVRYIGGC CGFEPYHIRA
310 320 330 340 350
IAEELAPERG FLPPASEKHG IWGSGLDMHT KPWIRARARR EYWETLLPAS
360
GRPFCPSLSK PDA
Length:363
Mass (Da):39,929
Last modified:October 11, 2004 - v1
Checksum:i403AAC188B979BFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079366 mRNA. Translation: AAH79366.1.
RefSeqiNP_001014278.2. NM_001014256.2.
UniGeneiRn.8494.

Genome annotation databases

GeneIDi365972.
KEGGirno:365972.
UCSCiRGD:1359418. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079366 mRNA. Translation: AAH79366.1.
RefSeqiNP_001014278.2. NM_001014256.2.
UniGeneiRn.8494.

3D structure databases

ProteinModelPortaliQ68FT5.
SMRiQ68FT5. Positions 10-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000058419.

PTM databases

iPTMnetiQ68FT5.

Proteomic databases

PaxDbiQ68FT5.
PRIDEiQ68FT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi365972.
KEGGirno:365972.
UCSCiRGD:1359418. rat.

Organism-specific databases

CTDi23743.
RGDi1359418. Bhmt2.

Phylogenomic databases

eggNOGiKOG1579. Eukaryota.
COG0646. LUCA.
HOGENOMiHOG000231636.
HOVERGENiHBG080367.
InParanoidiQ68FT5.
KOiK00547.
PhylomeDBiQ68FT5.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00083.

Miscellaneous databases

PROiQ68FT5.

Family and domain databases

Gene3Di3.20.20.330. 1 hit.
InterProiIPR017226. Betaine-hCys_S-MeTrfase_BHMT.
IPR003726. HCY_dom.
[Graphical view]
PfamiPF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF037505. Betaine_HMT. 1 hit.
SUPFAMiSSF82282. SSF82282. 1 hit.
PROSITEiPS50970. HCY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiBHMT2_RAT
AccessioniPrimary (citable) accession number: Q68FT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.