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Protein

Cytosol aminopeptidase

Gene

Lap3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of N-terminal proline from a peptide.

Cofactori

Note: Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi282 – 2821Zinc 2By similarity
Metal bindingi287 – 2871Zinc 1By similarity
Metal bindingi287 – 2871Zinc 2By similarity
Active sitei294 – 2941By similarity
Metal bindingi305 – 3051Zinc 2By similarity
Metal bindingi364 – 3641Zinc 1By similarity
Metal bindingi366 – 3661Zinc 1By similarity
Metal bindingi366 – 3661Zinc 2By similarity
Active sitei368 – 3681By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase 3
Short name:
LAP-3
Leucyl aminopeptidase
Proline aminopeptidase (EC:3.4.11.5)
Prolyl aminopeptidase
Gene namesi
Name:Lap3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1307985. Lap3.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • mitochondrion Source: Ensembl
  • nucleoplasm Source: Ensembl
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Cytosol aminopeptidasePRO_0000274146Add
BLAST
Isoform 2 (identifier: Q68FS4-2)
Initiator methionineiRemoved

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421PhosphoserineCombined sources
Modified residuei45 – 451N6-succinyllysineBy similarity
Modified residuei54 – 541PhosphoserineCombined sources
Modified residuei61 – 611N6-succinyllysineBy similarity
Modified residuei103 – 1031N6-succinyllysineBy similarity
Modified residuei180 – 1801PhosphoserineBy similarity
Modified residuei194 – 1941PhosphoserineBy similarity
Modified residuei221 – 2211N6-acetyllysine; alternateBy similarity
Modified residuei221 – 2211N6-succinyllysine; alternateBy similarity
Modified residuei238 – 2381PhosphoserineBy similarity
Modified residuei455 – 4551N6-acetyllysine; alternateBy similarity
Modified residuei455 – 4551N6-succinyllysine; alternateBy similarity
Modified residuei476 – 4761N6-succinyllysineBy similarity
Modified residuei489 – 4891N6-acetyllysine; alternateBy similarity
Modified residuei489 – 4891N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ68FS4.
PRIDEiQ68FS4.

PTM databases

iPTMnetiQ68FS4.
PhosphoSiteiQ68FS4.

Expressioni

Gene expression databases

GenevisibleiQ68FS4. RN.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004770.

Structurei

3D structure databases

ProteinModelPortaliQ68FS4.
SMRiQ68FS4. Positions 33-518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243129.
HOVERGENiHBG003320.
InParanoidiQ68FS4.
KOiK11142.
OMAiGIVGMKS.
OrthoDBiEOG7F24SG.
PhylomeDBiQ68FS4.
TreeFamiTF314954.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q68FS4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYLLPLPAAA RVALRRLGVR GLWDRGLSTA DMTKGLVLGI YSKDKDDDVP
60 70 80 90 100
QFTSAGENFN KLVSGRLREM LNISGPPLKA GKTRTFYGLH QDFPSVVVVG
110 120 130 140 150
LGKRSAGVDD QENWHEGKEN IRAAVAAGCR QVQDLELPSV EVDPCGDAQA
160 170 180 190 200
AAEGAVLGLY EYDDLKQKKK VAVSAKLHGS GDLEAWEKGV LFASGQNLAR
210 220 230 240 250
QLMESPANEM TPTRFAEVIE KNLKSASSKT EVHIRTKSWI EEQEMGSFLS
260 270 280 290 300
VAKGSEEPPV FLEIHYTGSP NATEAPLVFV GKGITFDSGG ISIKASANMD
310 320 330 340 350
LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV
360 370 380 390 400
RARNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVIINAA TLTGAMDVAL
410 420 430 440 450
GSGATGVFTN SSWLWNKLFE ASVETGDRVW RMPLFEHYTR QVIDCQLADV
460 470 480 490 500
NNLGKYRSAG ACTAAAFLRE FVTHTKWAHL DIAGVMTNKD EIPYLRKGMS
510
GRPTRTLIEF LLRFSKDSS
Length:519
Mass (Da):56,150
Last modified:October 11, 2004 - v1
Checksum:i0D4DFDC93D0CFF9D
GO
Isoform 2 (identifier: Q68FS4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.
Show »
Length:488
Mass (Da):52,759
Checksum:iADBF6E2235FB3D94
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3131Missing in isoform 2. CuratedVSP_022633Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079381 mRNA. Translation: AAH79381.1.
RefSeqiNP_001011910.1. NM_001011910.1. [Q68FS4-1]
UniGeneiRn.99790.

Genome annotation databases

EnsembliENSRNOT00000004770; ENSRNOP00000004770; ENSRNOG00000003289. [Q68FS4-1]
GeneIDi289668.
KEGGirno:289668.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079381 mRNA. Translation: AAH79381.1.
RefSeqiNP_001011910.1. NM_001011910.1. [Q68FS4-1]
UniGeneiRn.99790.

3D structure databases

ProteinModelPortaliQ68FS4.
SMRiQ68FS4. Positions 33-518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004770.

PTM databases

iPTMnetiQ68FS4.
PhosphoSiteiQ68FS4.

Proteomic databases

PaxDbiQ68FS4.
PRIDEiQ68FS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004770; ENSRNOP00000004770; ENSRNOG00000003289. [Q68FS4-1]
GeneIDi289668.
KEGGirno:289668.

Organism-specific databases

CTDi51056.
RGDi1307985. Lap3.

Phylogenomic databases

eggNOGiKOG2597. Eukaryota.
COG0260. LUCA.
GeneTreeiENSGT00530000063255.
HOGENOMiHOG000243129.
HOVERGENiHBG003320.
InParanoidiQ68FS4.
KOiK11142.
OMAiGIVGMKS.
OrthoDBiEOG7F24SG.
PhylomeDBiQ68FS4.
TreeFamiTF314954.

Miscellaneous databases

PROiQ68FS4.

Gene expression databases

GenevisibleiQ68FS4. RN.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. Lubec G., Afjehi-Sadat L., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 69-79; 85-103; 189-200; 283-294; 322-342; 369-384; 441-455; 458-469; 477-496 AND 506-513, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-54, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAMPL_RAT
AccessioniPrimary (citable) accession number: Q68FS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2004
Last modified: July 6, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.