Reviewed, UniProtKB/Swiss-Prot Q68FS4 (AMPL_RAT)

Names and origin

Protein names

Recommended name:
    Cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
    Leucyl aminopeptidase
    Leucine aminopeptidase 3
      Short name=LAP
    Proline aminopeptidase
    EC=3.4.11.5
    Prolyl aminopeptidase

Gene names

Name:

Lap3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Release of N-terminal proline from a peptide.
Cofactor	Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities By similarity.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
Ligand	Magnesium Manganese Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Protease
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q68FS4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q68FS4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: The initiator methionine is removed. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 519	519	Cytosol aminopeptidase		PRO_0000274146
Sites
	Active site	294	1	By similarity
	Active site	368	1	By similarity
	Metal binding	282	1	Zinc 2 By similarity
	Metal binding	287	1	Zinc 1 By similarity
	Metal binding	287	1	Zinc 2 By similarity
	Metal binding	305	1	Zinc 2 By similarity
	Metal binding	364	1	Zinc 1 By similarity
	Metal binding	366	1	Zinc 1 By similarity
	Metal binding	366	1	Zinc 2 By similarity
Amino acid modifications
	Modified residue	33	1	N-acetylthreonine By similarity
	Modified residue	221	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1 – 31	31	Missing in isoform 2.		VSP_022633

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 0D4DFDC93D0CFF9D	FASTA	519	56,150
	10 20 30 40 50 60 MYLLPLPAAA RVALRRLGVR GLWDRGLSTA DMTKGLVLGI YSKDKDDDVP QFTSAGENFN 70 80 90 100 110 120 KLVSGRLREM LNISGPPLKA GKTRTFYGLH QDFPSVVVVG LGKRSAGVDD QENWHEGKEN 130 140 150 160 170 180 IRAAVAAGCR QVQDLELPSV EVDPCGDAQA AAEGAVLGLY EYDDLKQKKK VAVSAKLHGS 190 200 210 220 230 240 GDLEAWEKGV LFASGQNLAR QLMESPANEM TPTRFAEVIE KNLKSASSKT EVHIRTKSWI 250 260 270 280 290 300 EEQEMGSFLS VAKGSEEPPV FLEIHYTGSP NATEAPLVFV GKGITFDSGG ISIKASANMD 310 320 330 340 350 360 LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV RARNGKTIQV 370 380 390 400 410 420 DNTDAEGRLI LADALCYAHT FNPKVIINAA TLTGAMDVAL GSGATGVFTN SSWLWNKLFE 430 440 450 460 470 480 ASVETGDRVW RMPLFEHYTR QVIDCQLADV NNLGKYRSAG ACTAAAFLRE FVTHTKWAHL 490 500 510 DIAGVMTNKD EIPYLRKGMS GRPTRTLIEF LLRFSKDSS
	Isoform 2. Checksum: ADBF6E2235FB3D94	FASTA	488	52,759
	10 20 30 40 50 60 MTKGLVLGIY SKDKDDDVPQ FTSAGENFNK LVSGRLREML NISGPPLKAG KTRTFYGLHQ 70 80 90 100 110 120 DFPSVVVVGL GKRSAGVDDQ ENWHEGKENI RAAVAAGCRQ VQDLELPSVE VDPCGDAQAA 130 140 150 160 170 180 AEGAVLGLYE YDDLKQKKKV AVSAKLHGSG DLEAWEKGVL FASGQNLARQ LMESPANEMT 190 200 210 220 230 240 PTRFAEVIEK NLKSASSKTE VHIRTKSWIE EQEMGSFLSV AKGSEEPPVF LEIHYTGSPN 250 260 270 280 290 300 ATEAPLVFVG KGITFDSGGI SIKASANMDL MRADMGGAAT ICSAIVSAAK LNLPINIIGL 310 320 330 340 350 360 APLCENMPSG KANKPGDVVR ARNGKTIQVD NTDAEGRLIL ADALCYAHTF NPKVIINAAT 370 380 390 400 410 420 LTGAMDVALG SGATGVFTNS SWLWNKLFEA SVETGDRVWR MPLFEHYTRQ VIDCQLADVN 430 440 450 460 470 480 NLGKYRSAGA CTAAAFLREF VTHTKWAHLD IAGVMTNKDE IPYLRKGMSG RPTRTLIEFL LRFSKDSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	Lubec G., Afjehi-Sadat L., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 69-79; 85-103; 189-200; 283-294; 322-342; 369-384; 441-455; 458-469; 477-496 AND 506-513, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079381 mRNA. Translation: AAH79381.1.
IPI	IPI00471530. IPI00829505.
RefSeq	NP_001011910.1.
UniGene	Rn.99790
3D structure databases
HSSP	HSSP built from PDB template 1LAP based on UniProtKB P00727.
SMR	Q68FS4. Positions 33-518.
ModBase	Search...
Protein-protein interaction databases
STRING	Q68FS4.
Protein family/group databases
MEROPS	M17.001.
Genome annotation databases
Ensembl	ENSRNOT00000004770; ENSRNOP00000004770; ENSRNOG00000003289; Rattus norvegicus. [Genome view]
GeneID	289668.
KEGG	rno:289668.
UCSC	NM_001011910. rat.
Organism-specific databases
CTD	289668.
RGD	1307985. Lap3.
Phylogenomic databases
eggNOG	maNOG07537.
HOVERGEN	Q68FS4.
InParanoid	Q68FS4.
OMA	GIYSKDK.
OrthoDB	EOG9WQ3NS.
PhylomeDB	Q68FS4.
Enzyme and pathway databases
BRENDA	3.4.11.1. 248. 3.4.11.5. 248.
Gene expression databases
ArrayExpress	Q68FS4.
Genevestigator	Q68FS4.
Family and domain databases
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	630137.

Entry information

Entry name

AMPL_RAT

Accession

Primary (citable) accession number: Q68FS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 11, 2004
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)