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Protein

Cytosolic Fe-S cluster assembly factor NUBP2

Gene

Nubp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi196 – 1961Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi199 – 1991Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 298ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotation
Alternative name(s):
Nucleotide-binding protein 2UniRule annotation
Short name:
NBP 2UniRule annotation
Gene namesi
Name:Nubp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1305113. Nubp2.

Subcellular locationi

  • Nucleus UniRule annotation
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation
  • Cytoplasm By similarity
  • Cytoplasmcytoskeletoncilium axoneme By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing center By similarity

  • Note: Enriched at the centrosomes during mitosis. Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis (By similarity). Localized at centrioles and in the nucleus at interphase (By similarity). Colocalizes with nubp-1 at prometaphase (By similarity).UniRule annotationBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Cytosolic Fe-S cluster assembly factor NUBP2PRO_0000288717Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineUniRule annotationBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ68FS1.
PRIDEiQ68FS1.

Expressioni

Gene expression databases

GenevisibleiQ68FS1. RN.

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.UniRule annotationBy similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020504.

Structurei

3D structure databases

ProteinModelPortaliQ68FS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ68FS1.
OMAiFPKSTAY.
OrthoDBiEOG7D59NZ.
PhylomeDBiQ68FS1.
TreeFamiTF354321.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03039. NUBP2.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68FS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAAEPGNL AGVRHIILVL SGKGGVGKST ISTELALALR HQGKKVGILD
60 70 80 90 100
VDLCGPSIPH MLHAQGKAVH QCDSGWVPVF VDQEQSISLM SVGFLLENPD
110 120 130 140 150
EAVVWRGPKK HALIKQFVSD VAWGELDYLV VDTPPGTSDE HMATVEALRP
160 170 180 190 200
YKPLGALVVT TPQAVSIGDV RRELTFCKKT GLQVIGVIEN MSGFACPHCA
210 220 230 240 250
ECTNVFSSGG GEELARLAGV PFLGSVPLDP QLTRSLEEGR DFIQEFPKST
260 270
AYSALTSIAH KVLHQMPALC S
Length:271
Mass (Da):28,926
Last modified:October 11, 2004 - v1
Checksum:i1D33E2A6EDA061DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079386 mRNA. Translation: AAH79386.1.
RefSeqiNP_001011891.1. NM_001011891.1.
UniGeneiRn.203395.

Genome annotation databases

EnsembliENSRNOT00000020504; ENSRNOP00000020504; ENSRNOG00000015090.
GeneIDi287125.
KEGGirno:287125.
UCSCiRGD:1305113. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079386 mRNA. Translation: AAH79386.1.
RefSeqiNP_001011891.1. NM_001011891.1.
UniGeneiRn.203395.

3D structure databases

ProteinModelPortaliQ68FS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020504.

Proteomic databases

PaxDbiQ68FS1.
PRIDEiQ68FS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020504; ENSRNOP00000020504; ENSRNOG00000015090.
GeneIDi287125.
KEGGirno:287125.
UCSCiRGD:1305113. rat.

Organism-specific databases

CTDi10101.
RGDi1305113. Nubp2.

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00550000075057.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiQ68FS1.
OMAiFPKSTAY.
OrthoDBiEOG7D59NZ.
PhylomeDBiQ68FS1.
TreeFamiTF354321.

Miscellaneous databases

NextBioi625558.
PROiQ68FS1.

Gene expression databases

GenevisibleiQ68FS1. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35.
MF_03039. NUBP2.
InterProiIPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028600. NUBP2/Cfd1_eukaryotes.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiPF10609. ParA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS01215. MRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 15-23; 29-40 AND 249-261, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiNUBP2_RAT
AccessioniPrimary (citable) accession number: Q68FS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2004
Last modified: January 20, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.