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Protein

Cytosolic Fe-S cluster assembly factor NUBP2

Gene

Nubp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi199Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 29ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP2UniRule annotation
Alternative name(s):
Nucleotide-binding protein 2UniRule annotation
Short name:
NBP 2UniRule annotation
Gene namesi
Name:Nubp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1305113 Nubp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002887171 – 271Cytosolic Fe-S cluster assembly factor NUBP2Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineUniRule annotationBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ68FS1
PRIDEiQ68FS1

Expressioni

Gene expression databases

BgeeiENSRNOG00000015090
GenevisibleiQ68FS1 RN

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.UniRule annotationBy similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020504

Structurei

3D structure databases

ProteinModelPortaliQ68FS1
SMRiQ68FS1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ Eukaryota
COG0489 LUCA
GeneTreeiENSGT00550000075057
HOGENOMiHOG000079916
HOVERGENiHBG051027
InParanoidiQ68FS1
OMAiRELTFCK
OrthoDBiEOG091G0DY5
PhylomeDBiQ68FS1
TreeFamiTF354321

Family and domain databases

CDDicd02037 MRP-like, 1 hit
HAMAPiMF_02040 Mrp_NBP35, 1 hit
MF_03039 NUBP2, 1 hit
InterProiView protein in InterPro
IPR019591 Mrp/NBP35_ATP-bd
IPR000808 Mrp_CS
IPR028600 NUBP2/Cfd1_eukaryotes
IPR027417 P-loop_NTPase
IPR033756 YlxH/NBP35
PANTHERiPTHR23264 PTHR23264, 1 hit
PfamiView protein in Pfam
PF10609 ParA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS01215 MRP, 1 hit

Sequencei

Sequence statusi: Complete.

Q68FS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAAEPGNL AGVRHIILVL SGKGGVGKST ISTELALALR HQGKKVGILD
60 70 80 90 100
VDLCGPSIPH MLHAQGKAVH QCDSGWVPVF VDQEQSISLM SVGFLLENPD
110 120 130 140 150
EAVVWRGPKK HALIKQFVSD VAWGELDYLV VDTPPGTSDE HMATVEALRP
160 170 180 190 200
YKPLGALVVT TPQAVSIGDV RRELTFCKKT GLQVIGVIEN MSGFACPHCA
210 220 230 240 250
ECTNVFSSGG GEELARLAGV PFLGSVPLDP QLTRSLEEGR DFIQEFPKST
260 270
AYSALTSIAH KVLHQMPALC S
Length:271
Mass (Da):28,926
Last modified:October 11, 2004 - v1
Checksum:i1D33E2A6EDA061DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079386 mRNA Translation: AAH79386.1
RefSeqiNP_001011891.1, NM_001011891.1
UniGeneiRn.203395

Genome annotation databases

EnsembliENSRNOT00000020504; ENSRNOP00000020504; ENSRNOG00000015090
GeneIDi287125
KEGGirno:287125
UCSCiRGD:1305113 rat

Similar proteinsi

Entry informationi

Entry nameiNUBP2_RAT
AccessioniPrimary (citable) accession number: Q68FS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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