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Q68FR9 (EF1D_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Elongation factor 1-delta
Short name=EF-1-delta
Gene names
Name:Eef1d
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome By similarity.

Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE) By similarity.

Subunit structure

EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma. Isoform 2 interacts with HSF1 and NFE2L2 By similarity.

Subcellular location

Isoform 2: Nucleus By similarity.

Sequence similarities

Belongs to the EF-1-beta/EF-1-delta family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q68FR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q68FR9-2)

Also known as: eEF1BdeltaL;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRSGKASCAL...SMSSLRPKKM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 281280Elongation factor 1-delta
PRO_0000382456

Regions

Region80 – 11536Leucine-zipper By similarity
Region173 – 281109Catalytic (GEF) By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue171N6-acetyllysine By similarity
Modified residue1071N6-acetyllysine By similarity
Modified residue1171N6-acetyllysine By similarity
Modified residue1291Phosphothreonine By similarity
Modified residue1331Phosphoserine By similarity
Modified residue1471Phosphothreonine By similarity
Modified residue1621Phosphoserine; by CK2 Ref.3

Natural variations

Alternative sequence11M → MRSGKASCALETVWEDKHKY EEAERRFHEHEATQAAAASV QQLLAEVPAVNGPSSQEDAE DTDEAETPNTSSRSDPRKSH ECKKPLQKKRKRSPKSWLGQ ADLALVGLSADHVWLDKPLF DQAESSYRQRLADVAAQAAQ SPALAPRGPCTHGSHVACHH VTWGIWVNKSCFDQAERAFV EWSQALLLAAEGSHREGTPD TGQQAVTPDLALACQPCPPA NGQPPLGSLQALVREVWLEK PRYDAAERGFYEALFDGHPP GKVRLQERASQAEGTRRGRR DRRSRNTVGNKRAGSKRADG EAPSALPYWYFLHKDAEAPW LSKPTYDSAECRHHAAEALR IAWRLEAASLAHRPTPRSGP SMSSLRPKKM in isoform 2.
VSP_037887

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 1, 2009. Version 2.
Checksum: E77BE1BD8BACBA7D

FASTA28131,330
        10         20         30         40         50         60 
MATNFLMHEK IWFDKFKYDD AERRFYEQMN GPVTAGSRQE NGASVILRDI ARARENIQKS 

        70         80         90        100        110        120 
LAGSSGPGAS SGPGGDHSDL IVRIASLEVE NQNLRGVVQD LQQAISKLEV RLSTLEKSSP 

       130        140        150        160        170        180 
THRATAPQTQ HVSPMRQVEP PAKKGATPAE DDEDNDIDLF GSDEEEEDKE AARLREERLR 

       190        200        210        220        230        240 
QYAEKKAKKP TLVAKSSILL DVKPWDDETD MAQLETCVRS IQLDGLVWGA SKLVPVGYGI 

       250        260        270        280 
RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I

« Hide

Isoform 2 (eEF1BdeltaL) [UniParc].

Checksum: 13765072C5011A6C
Show »

FASTA65072,128

References

[1]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[3]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, MASS SPECTROMETRY (ISOFORM 2).
Tissue: Renal collecting duct.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CH473950 Genomic DNA. Translation: EDM16037.1.
BC079391 mRNA. Translation: AAH79391.1.
IPIIPI00471525.
IPI00944235.
RefSeqNP_001013122.1. NM_001013104.1.
UniGeneRn.71883.

3D structure databases

HSSPHSSP built from PDB template 1B64 based on UniProtKB P24534.
ModBaseSearch...

Protein-protein interaction databases

IntActQ68FR9. 1 interaction.
STRING10116.ENSRNOP00000034828.

PTM databases

PhosphoSiteQ68FR9.

Proteomic databases

PaxDbQ68FR9.
PRIDEQ68FR9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000011260; ENSRNOP00000011260; ENSRNOG00000021638.
ENSRNOT00000029456; ENSRNOP00000034828; ENSRNOG00000021638.
GeneID300033.
KEGGrno:300033.
UCSCRGD:621174. rat.

Organism-specific databases

CTD1936.
RGD621174. Eef1d.

Phylogenomic databases

eggNOGCOG2092.
GeneTreeENSGT00390000011747.
HOGENOMHOG000139586.
InParanoidQ68FR9.
KOK15410.
OrthoDBEOG4CJVGT.

Gene expression databases

ArrayExpressQ68FR9.
GenevestigatorQ68FR9.

Family and domain databases

Gene3D3.30.70.60. 1 hit.
InterProIPR018940. EF-1_beta_acid_region_euk.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
IPR014038. Transl_elong_fac_EF1B_bsu/dsu.
[Graphical view]
PfamPF10587. EF-1_beta_acid. 1 hit.
PF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTSM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMSSF54984. Transl_elong_EF1B_B/D_G_exch. 1 hit.
PROSITEPS00825. EF1BD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio646165.

Entry information

Entry nameEF1D_RAT
AccessionPrimary (citable) accession number: Q68FR9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: May 1, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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