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Protein

Elongation factor 1-delta

Gene

Eef1d

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.By similarity
Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-156842. Eukaryotic Translation Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-delta
Short name:
EF-1-delta
Gene namesi
Name:Eef1d
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621174. Eef1d.

Subcellular locationi

Isoform 2 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 281280Elongation factor 1-deltaPRO_0000382456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei17 – 171N6-acetyllysineBy similarity
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei106 – 1061PhosphoserineCombined sources
Modified residuei107 – 1071N6-acetyllysineBy similarity
Modified residuei117 – 1171N6-acetyllysine; alternateBy similarity
Modified residuei117 – 1171N6-succinyllysine; alternateBy similarity
Modified residuei129 – 1291PhosphothreonineBy similarity
Modified residuei133 – 1331PhosphoserineCombined sources
Modified residuei147 – 1471PhosphothreonineCombined sources
Modified residuei162 – 1621Phosphoserine; by CK2Combined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ68FR9.
PRIDEiQ68FR9.

PTM databases

iPTMnetiQ68FR9.
PhosphoSiteiQ68FR9.

Expressioni

Gene expression databases

GenevisibleiQ68FR9. RN.

Interactioni

Subunit structurei

EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma. Isoform 2 interacts with HSF1 and NFE2L2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi256417. 1 interaction.
IntActiQ68FR9. 1 interaction.
STRINGi10116.ENSRNOP00000034828.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 11536Leucine-zipperBy similarityAdd
BLAST
Regioni173 – 281109Catalytic (GEF)By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the EF-1-beta/EF-1-delta family.Curated

Phylogenomic databases

eggNOGiKOG1668. Eukaryota.
COG2092. LUCA.
GeneTreeiENSGT00390000011747.
HOGENOMiHOG000139586.
InParanoidiQ68FR9.
KOiK15410.
OMAiWLEKPRY.
OrthoDBiEOG7RZ5QW.
PhylomeDBiQ68FR9.

Family and domain databases

Gene3Di3.30.70.60. 1 hit.
InterProiIPR018940. EF-1_beta_acid_region_euk.
IPR014038. EF1B_bsu/dsu_GNE.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
[Graphical view]
PfamiPF10587. EF-1_beta_acid. 1 hit.
PF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTiSM01182. EF-1_beta_acid. 1 hit.
SM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMiSSF54984. SSF54984. 1 hit.
PROSITEiPS00825. EF1BD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68FR9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATNFLMHEK IWFDKFKYDD AERRFYEQMN GPVTAGSRQE NGASVILRDI
60 70 80 90 100
ARARENIQKS LAGSSGPGAS SGPGGDHSDL IVRIASLEVE NQNLRGVVQD
110 120 130 140 150
LQQAISKLEV RLSTLEKSSP THRATAPQTQ HVSPMRQVEP PAKKGATPAE
160 170 180 190 200
DDEDNDIDLF GSDEEEEDKE AARLREERLR QYAEKKAKKP TLVAKSSILL
210 220 230 240 250
DVKPWDDETD MAQLETCVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE
260 270 280
DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I
Length:281
Mass (Da):31,330
Last modified:September 1, 2009 - v2
Checksum:iE77BE1BD8BACBA7D
GO
Isoform 2 (identifier: Q68FR9-2) [UniParc]FASTAAdd to basket

Also known as: eEF1BdeltaL

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRSGKASCAL...SMSSLRPKKM

Show »
Length:650
Mass (Da):72,128
Checksum:i13765072C5011A6C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MRSGKASCALETVWEDKHKY EEAERRFHEHEATQAAAASV QQLLAEVPAVNGPSSQEDAE DTDEAETPNTSSRSDPRKSH ECKKPLQKKRKRSPKSWLGQ ADLALVGLSADHVWLDKPLF DQAESSYRQRLADVAAQAAQ SPALAPRGPCTHGSHVACHH VTWGIWVNKSCFDQAERAFV EWSQALLLAAEGSHREGTPD TGQQAVTPDLALACQPCPPA NGQPPLGSLQALVREVWLEK PRYDAAERGFYEALFDGHPP GKVRLQERASQAEGTRRGRR DRRSRNTVGNKRAGSKRADG EAPSALPYWYFLHKDAEAPW LSKPTYDSAECRHHAAEALR IAWRLEAASLAHRPTPRSGP SMSSLRPKKM in isoform 2. 1 PublicationVSP_037887

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473950 Genomic DNA. Translation: EDM16037.1.
BC079391 mRNA. Translation: AAH79391.1.
RefSeqiNP_001013122.1. NM_001013104.1. [Q68FR9-2]
XP_008763779.1. XM_008765557.1. [Q68FR9-1]
XP_008763780.1. XM_008765558.1. [Q68FR9-1]
XP_008763781.1. XM_008765559.1. [Q68FR9-1]
UniGeneiRn.71883.

Genome annotation databases

EnsembliENSRNOT00000029456; ENSRNOP00000034828; ENSRNOG00000021638. [Q68FR9-2]
GeneIDi300033.
KEGGirno:300033.
UCSCiRGD:621174. rat. [Q68FR9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473950 Genomic DNA. Translation: EDM16037.1.
BC079391 mRNA. Translation: AAH79391.1.
RefSeqiNP_001013122.1. NM_001013104.1. [Q68FR9-2]
XP_008763779.1. XM_008765557.1. [Q68FR9-1]
XP_008763780.1. XM_008765558.1. [Q68FR9-1]
XP_008763781.1. XM_008765559.1. [Q68FR9-1]
UniGeneiRn.71883.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi256417. 1 interaction.
IntActiQ68FR9. 1 interaction.
STRINGi10116.ENSRNOP00000034828.

PTM databases

iPTMnetiQ68FR9.
PhosphoSiteiQ68FR9.

Proteomic databases

PaxDbiQ68FR9.
PRIDEiQ68FR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029456; ENSRNOP00000034828; ENSRNOG00000021638. [Q68FR9-2]
GeneIDi300033.
KEGGirno:300033.
UCSCiRGD:621174. rat. [Q68FR9-1]

Organism-specific databases

CTDi1936.
RGDi621174. Eef1d.

Phylogenomic databases

eggNOGiKOG1668. Eukaryota.
COG2092. LUCA.
GeneTreeiENSGT00390000011747.
HOGENOMiHOG000139586.
InParanoidiQ68FR9.
KOiK15410.
OMAiWLEKPRY.
OrthoDBiEOG7RZ5QW.
PhylomeDBiQ68FR9.

Enzyme and pathway databases

ReactomeiR-RNO-156842. Eukaryotic Translation Elongation.

Miscellaneous databases

PROiQ68FR9.

Gene expression databases

GenevisibleiQ68FR9. RN.

Family and domain databases

Gene3Di3.30.70.60. 1 hit.
InterProiIPR018940. EF-1_beta_acid_region_euk.
IPR014038. EF1B_bsu/dsu_GNE.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
[Graphical view]
PfamiPF10587. EF-1_beta_acid. 1 hit.
PF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTiSM01182. EF-1_beta_acid. 1 hit.
SM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMiSSF54984. SSF54984. 1 hit.
PROSITEiPS00825. EF1BD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; SER-133; THR-147 AND SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEF1D_RAT
AccessioniPrimary (citable) accession number: Q68FR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: June 8, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.