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Protein

RNA polymerase II-associated protein 3

Gene

Rpap3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation.By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II-associated protein 3
Gene namesi
Name:Rpap3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1303036. Rpap3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 659658RNA polymerase II-associated protein 3PRO_0000302796Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei116 – 1161PhosphoserineBy similarity
Modified residuei119 – 1191PhosphoserineBy similarity
Modified residuei121 – 1211PhosphoserineCombined sources
Modified residuei474 – 4741PhosphoserineBy similarity
Cross-linki491 – 491Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ68FQ7.

Expressioni

Gene expression databases

GenevisibleiQ68FQ7. RN.

Interactioni

Subunit structurei

Tightly associated with the RNA polymerase II complex. Component of the R2TP complex composed at least of PIHD1, RUVBL1, RUVBL2 and RPAP3. Interacts with PIH1D1.By similarity

Protein-protein interaction databases

IntActiQ68FQ7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ68FQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati8 – 4134TPR 1Add
BLAST
Repeati133 – 16634TPR 2Add
BLAST
Repeati168 – 20033TPR 3Add
BLAST
Repeati201 – 23434TPR 4Add
BLAST
Repeati282 – 31534TPR 5Add
BLAST
Repeati317 – 34933TPR 6Add
BLAST
Repeati350 – 38334TPR 7Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 708Poly-Lys

Sequence similaritiesi

Belongs to the RPAP3 family.Curated
Contains 7 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00840000129761.
HOGENOMiHOG000154181.
HOVERGENiHBG059892.
InParanoidiQ68FQ7.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR025986. RPAP3-like_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13877. RPAP3_C. 1 hit.
PF00515. TPR_1. 1 hit.
PF07719. TPR_2. 1 hit.
PF13176. TPR_7. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSTSKAVEL QLQVKHNAEE LQDFMRDLEH WEKTMRQKDL ELRRQSGVPE
60 70 80 90 100
ENLPPIRNGS FRKKKKRKTK DSSKKTKEEN TKNRIKSFDY DAWAKLDVDS
110 120 130 140 150
ILDELDKEDS THDSVSQESE SDEDGVRVDS QKALVLKEKG NKYFKQGKYD
160 170 180 190 200
EAIECYTKGM DADPYNPVLP TNRASAYFRL KKFAVAESDC NLAIALSRSY
210 220 230 240 250
TKAYARRGAA RFALQKLEDA RKDYVKVLEL EPDNFEATNE LRKIDQALTS
260 270 280 290 300
KENSHPKDIA AVIKPAEGER KANEDQRGRQ KAIAEKDLGN GFFKEGKYEQ
310 320 330 340 350
AIECYTRGIA ADSTNALLPA NRAMAYLKVQ KYEEAERDCT QAILLDGSYS
360 370 380 390 400
KAFARRGTAR TFLGKINEAK QDFETVLLLE PGNKQAVTEL SRIKKELIEK
410 420 430 440 450
GRWDDVFLDS TQRHNVVKPV DSPHRGSPKA LKKVFIEETG NLIESVDAPE
460 470 480 490 500
SSATVPESDR AAVAVDTGRK KDFSQGDSVS SGETPRAKVL KIEAVGDSSA
510 520 530 540 550
PQAQVDVKQG VRQSVSEKTS VRVAQTPGQL AAVVLPPVPA NSFQLESDFR
560 570 580 590 600
QLRSSPEMLY QYVKKIEPSL YPKLFQKNLD PDVFNQIIKI LHDFYVEREK
610 620 630 640 650
PALIFEVLER LSQLRRFDMA VMFMSGTERE LTKVLFNHLE KSELKEDSVE

ELKKRYGGG
Length:659
Mass (Da):74,645
Last modified:October 11, 2004 - v1
Checksum:i519D6C55825593B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079414 mRNA. Translation: AAH79414.1.
RefSeqiNP_001004243.1. NM_001004243.1.
XP_008763912.1. XM_008765690.1.
UniGeneiRn.49051.

Genome annotation databases

EnsembliENSRNOT00000083926; ENSRNOP00000074251; ENSRNOG00000053405.
GeneIDi300189.
KEGGirno:300189.
UCSCiRGD:1303036. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079414 mRNA. Translation: AAH79414.1.
RefSeqiNP_001004243.1. NM_001004243.1.
XP_008763912.1. XM_008765690.1.
UniGeneiRn.49051.

3D structure databases

ProteinModelPortaliQ68FQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ68FQ7. 1 interaction.

Proteomic databases

PRIDEiQ68FQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000083926; ENSRNOP00000074251; ENSRNOG00000053405.
GeneIDi300189.
KEGGirno:300189.
UCSCiRGD:1303036. rat.

Organism-specific databases

CTDi79657.
RGDi1303036. Rpap3.

Phylogenomic databases

GeneTreeiENSGT00840000129761.
HOGENOMiHOG000154181.
HOVERGENiHBG059892.
InParanoidiQ68FQ7.

Miscellaneous databases

NextBioi646490.
PROiQ68FQ7.

Gene expression databases

GenevisibleiQ68FQ7. RN.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR025986. RPAP3-like_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13877. RPAP3_C. 1 hit.
PF00515. TPR_1. 1 hit.
PF07719. TPR_2. 1 hit.
PF13176. TPR_7. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRPAP3_RAT
AccessioniPrimary (citable) accession number: Q68FQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 11, 2004
Last modified: May 11, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.