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Protein

Serum paraoxonase/lactonase 3

Gene

Pon3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents (By similarity).By similarity

Catalytic activityi

A phenyl acetate + H2O = a phenol + acetate.
An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.
An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine.

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Calcium 1; catalyticBy similarity
Metal bindingi54 – 541Calcium 2By similarity
Active sitei114 – 1141Proton acceptorBy similarity
Metal bindingi116 – 1161Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi167 – 1671Calcium 1; catalyticBy similarity
Metal bindingi168 – 1681Calcium 2By similarity
Metal bindingi223 – 2231Calcium 1; catalyticBy similarity
Metal bindingi268 – 2681Calcium 1; catalyticBy similarity
Metal bindingi269 – 2691Calcium 1; catalyticBy similarity

GO - Molecular functioni

  • 3,4-dihydrocoumarin hydrolase activity Source: RGD
  • acyl-L-homoserine-lactone lactonohydrolase activity Source: UniProtKB-EC
  • aryldialkylphosphatase activity Source: UniProtKB-EC
  • arylesterase activity Source: RGD
  • dihydrocoumarin hydrolase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • coumarin catabolic process Source: RGD
  • negative regulation of superoxide anion generation Source: Ensembl
  • phenylacetate catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.2. 5301.
3.1.1.25. 5301.
3.1.8.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Serum paraoxonase/lactonase 3 (EC:3.1.1.2, EC:3.1.1.81, EC:3.1.8.1)
Gene namesi
Name:Pon3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1302965. Pon3.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 354353Serum paraoxonase/lactonase 3PRO_0000223292Add
BLAST
Signal peptidei2 – ?Not cleavedSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 352By similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis
Modified residuei165 – 1651PhosphoserineBy similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.By similarity
The signal sequence is not cleaved.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ68FP2.
PRIDEiQ68FP2.

Expressioni

Gene expression databases

GenevisibleiQ68FP2. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

MINTiMINT-4655760.
STRINGi10116.ENSRNOP00000012050.

Structurei

3D structure databases

ProteinModelPortaliQ68FP2.
SMRiQ68FP2. Positions 23-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the paraoxonase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHDV. Eukaryota.
ENOG4111QK7. LUCA.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiQ68FP2.
KOiK01045.
OMAiQKYVYVA.
OrthoDBiEOG7GN2N3.
PhylomeDBiQ68FP2.
TreeFamiTF322436.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008364. Paraoxonase2.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01787. PARAOXONASE2.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKLVALTVL GASLALLGER LLNFRERVST SREIKPTEPQ NCHLIEGLEN
60 70 80 90 100
GSEDIDILPS GLAFISTGLK YPGMPSFAPD KPGRIFLMDL NEPYPKAQAL
110 120 130 140 150
EISDGFDQDS LNPHGISTFI DKDNTVYLYA VNHPHMDSTV EIFKFEEQPR
160 170 180 190 200
SLVHLKTIKH ELFESVNDIV VLGPEQFYAT RDHYFTSHFL VLLEMILDPH
210 220 230 240 250
WTSVLFYSPK EVKVVAQGFS SANGITVSLD QKYVYVADVT AKNIHIMKKH
260 270 280 290 300
NNWDLTPVKV IQLGTLVDNL TVDPATGDIL AGCHPNPMKL LIYNPEDPPG
310 320 330 340 350
SEVLRIQDPL SDNPRVSTLY SNNGSVLQGS TVASVYHKKM LIGTIFHKAL

YCEL
Length:354
Mass (Da):39,458
Last modified:October 11, 2004 - v1
Checksum:i913C28243E4B0D7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079466 mRNA. Translation: AAH79466.1.
RefSeqiNP_001004086.1. NM_001004086.1.
UniGeneiRn.16469.

Genome annotation databases

EnsembliENSRNOT00000012050; ENSRNOP00000012050; ENSRNOG00000009096.
GeneIDi312086.
KEGGirno:312086.
UCSCiRGD:1302965. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079466 mRNA. Translation: AAH79466.1.
RefSeqiNP_001004086.1. NM_001004086.1.
UniGeneiRn.16469.

3D structure databases

ProteinModelPortaliQ68FP2.
SMRiQ68FP2. Positions 23-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4655760.
STRINGi10116.ENSRNOP00000012050.

Proteomic databases

PaxDbiQ68FP2.
PRIDEiQ68FP2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012050; ENSRNOP00000012050; ENSRNOG00000009096.
GeneIDi312086.
KEGGirno:312086.
UCSCiRGD:1302965. rat.

Organism-specific databases

CTDi5446.
RGDi1302965. Pon3.

Phylogenomic databases

eggNOGiENOG410IHDV. Eukaryota.
ENOG4111QK7. LUCA.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiQ68FP2.
KOiK01045.
OMAiQKYVYVA.
OrthoDBiEOG7GN2N3.
PhylomeDBiQ68FP2.
TreeFamiTF322436.

Enzyme and pathway databases

BRENDAi3.1.1.2. 5301.
3.1.1.25. 5301.
3.1.8.1. 5301.

Miscellaneous databases

PROiQ68FP2.

Gene expression databases

GenevisibleiQ68FP2. RN.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008364. Paraoxonase2.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01787. PARAOXONASE2.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiPON3_RAT
AccessioniPrimary (citable) accession number: Q68FP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: October 11, 2004
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.