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Q68FP1 (GELS_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gelsolin
Alternative name(s):
Actin-depolymerizing factor
Short name=ADF
Brevin
Gene names
Name:Gsn
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length780 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis By similarity.

Subunit structure

Binds to actin and to fibronectin. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X By similarity. Interacts with the inactive form of EIF2AK2/PKR By similarity.

Subcellular location

Isoform 1: Secreted By similarity.

Isoform 2: Cytoplasmcytoskeleton By similarity. Note: A cytoplasmic form may also exist By similarity.

Post-translational modification

Phosphorylated on tyrosine residues in vitro By similarity.

Sequence similarities

Belongs to the villin/gelsolin family.

Contains 6 gelsolin-like repeats.

Ontologies

Keywords
   Biological processCilium biogenesis/degradation
   Cellular componentCytoplasm
Cytoskeleton
Secreted
   Coding sequence diversityAlternative initiation
   DomainRepeat
Signal
   LigandActin-binding
Calcium
Metal-binding
   Molecular functionActin capping
   PTMAcetylation
Disulfide bond
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament capping

Inferred from electronic annotation. Source: UniProtKB-KW

actin filament polymerization

Inferred from sequence or structural similarity. Source: UniProtKB

actin filament severing

Inferred from sequence or structural similarity. Source: UniProtKB

actin polymerization or depolymerization

Inferred from direct assay PubMed 16280379. Source: RGD

aging

Inferred from expression pattern PubMed 14652020. Source: RGD

apoptotic process

Inferred from direct assay PubMed 10698078. Source: RGD

cellular response to cadmium ion

Inferred from direct assay PubMed 16280379. Source: RGD

cilium morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

oligodendrocyte development

Inferred from expression pattern PubMed 7817780. Source: RGD

phosphatidylinositol-mediated signaling

Inferred from mutant phenotype PubMed 11861757. Source: RGD

regulation of cell adhesion

Inferred from mutant phenotype PubMed 11861757. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 12899944. Source: RGD

response to folic acid

Inferred from expression pattern PubMed 12899944. Source: RGD

tissue regeneration

Inferred from expression pattern PubMed 14574581. Source: RGD

vesicle-mediated transport

Inferred from electronic annotation. Source: Ensembl

wound healing

Inferred from expression pattern PubMed 2164930. Source: RGD

   Cellular_componentactin cytoskeleton

Inferred from direct assay PubMed 16280379. Source: RGD

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular region

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from direct assay PubMed 2829631. Source: RGD

extracellular vesicular exosome

Inferred from electronic annotation. Source: Ensembl

lamellipodium

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from direct assay PubMed 16280379. Source: RGD

protein complex

Inferred from direct assay PubMed 15823548. Source: RGD

ruffle

Inferred from direct assay PubMed 16280379. Source: RGD

   Molecular_functionactin binding

Inferred from direct assay PubMed 16280379. Source: RGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: Q68FP1-1)

Also known as: Secreted; Plasma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q68FP1-2)

Also known as: Cytoplasmic;

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 780755Gelsolin
PRO_0000288477

Regions

Repeat74 – 12451Gelsolin-like 1
Repeat196 – 23641Gelsolin-like 2
Repeat312 – 35443Gelsolin-like 3
Repeat451 – 50252Gelsolin-like 4
Repeat574 – 61441Gelsolin-like 5
Repeat677 – 71943Gelsolin-like 6
Region51 – 174124Actin-severing Potential
Region121 – 1244Actin-actin interfilament contact point By similarity
Region160 – 1678Polyphosphoinositide binding By similarity
Region186 – 1949Polyphosphoinositide binding By similarity
Region432 – 780349Actin-binding, Ca-sensitive Potential

Sites

Metal binding4691Calcium 1; via carbonyl oxygen By similarity
Metal binding4701Calcium 1 By similarity
Metal binding5001Calcium 1 By similarity
Metal binding5491Calcium 1; via carbonyl oxygen By similarity
Metal binding5891Calcium 2 By similarity
Metal binding5891Calcium 2; via carbonyl oxygen By similarity
Metal binding5901Calcium 2 By similarity
Metal binding6121Calcium 2 By similarity
Metal binding6941Calcium 3; via carbonyl oxygen By similarity
Metal binding6951Calcium 3 By similarity
Metal binding7171Calcium 3 By similarity

Amino acid modifications

Modified residue841Phosphotyrosine By similarity
Modified residue4071Phosphotyrosine By similarity
Modified residue4631Phosphotyrosine By similarity
Modified residue5821N6-acetyllysine By similarity
Modified residue6011Phosphotyrosine By similarity
Modified residue6491Phosphotyrosine By similarity
Disulfide bond213 ↔ 226 By similarity

Natural variations

Alternative sequence1 – 4949Missing in isoform 2.
VSP_036722

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Secreted) (Plasma) [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: E9B6A20C5A80D0E8

FASTA78086,068
        10         20         30         40         50         60 
MAPYCSSLRS ALLVLALCAL SPSHAATASR GRAQERAPQS RVSETRPSTM VVEHPEFLKA 

        70         80         90        100        110        120 
GKEPGLQIWR VEKFDLVPVP PNLYGDFFTG DAYVILKTVQ LRNGNLQYDL HYWLGNECSQ 

       130        140        150        160        170        180 
DESGAAAIFT VQLDDYLNGR AVQHREVQGF ESSTFQGYFK SGLKYKKGGV ASGFKHVVPN 

       190        200        210        220        230        240 
EVVVQRLFQV KGRRVVRATE VPVSWDSFNN GDCFILDLGN NIYQWCGSGS NKFERLKATQ 

       250        260        270        280        290        300 
VSKGIRDNER SGRAQVHVSE EGSEPEAMLQ VLGPKPDLPQ GTEDTAKEDA ANRRLAKLYK 

       310        320        330        340        350        360 
VSNSGGSMSV SLVADENPFA QSALRSEDCF ILDHGRDGKI FVWKGKQANM DERKAALKTA 

       370        380        390        400        410        420 
SDFISKMQYP RQTQVSVLPE GGETPLFKQF FKNWRDPDQT DGPGLSYLSS HIANVERVPF 

       430        440        450        460        470        480 
DAATLHTSTA MAAQHGMDDD GTGQKQIWRI EGSNKVLVDP ATYGQFYGGD SYIILYNYRH 

       490        500        510        520        530        540 
GGRQGQIIYN WQGAQSTQDE VAASAILTAQ LDEELGGTPV QSRVVQGKEP AHLMSLFGGK 

       550        560        570        580        590        600 
PMIIYKGGTS RDGGQTTPAS TRLFQVRASS SGATRAVEVM PKAGALNSND AFVLKTPSAA 

       610        620        630        640        650        660 
YLWVGTGASD AEKTGALELL KVLRAQHVQV EEGSEPDGFW EALGGKTAYR TSPRLKDKKM 

       670        680        690        700        710        720 
DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD TWDQVFVWVG KDSQEEEKTE 

       730        740        750        760        770        780 
ALTSAKRYIE TDPANRDRRT PITVVRQGFE PPSFVGWFLG WDDDYWSVDP LDRALAELAA 

« Hide

Isoform 2 (Cytoplasmic) [UniParc].

Checksum: 2AD1D7C83C98DFE0
Show »

FASTA73180,929

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[2]Lubec G., Diao W.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 301-325; 396-417; 583-595 AND 614-621, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC079472 mRNA. Translation: AAH79472.1.
RefSeqNP_001004080.1. NM_001004080.1.
XP_006234102.1. XM_006234040.1.
UniGeneRn.103770.

3D structure databases

ProteinModelPortalQ68FP1.
SMRQ68FP1. Positions 51-780.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid255439. 1 interaction.
IntActQ68FP1. 1 interaction.
STRING10116.ENSRNOP00000025857.

PTM databases

PhosphoSiteQ68FP1.

Proteomic databases

PaxDbQ68FP1.
PRIDEQ68FP1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025857; ENSRNOP00000025857; ENSRNOG00000018991. [Q68FP1-1]
GeneID296654.
KEGGrno:296654.
UCSCRGD:1303089. rat. [Q68FP1-1]

Organism-specific databases

CTD2934.
RGD1303089. Gsn.

Phylogenomic databases

eggNOGNOG304849.
GeneTreeENSGT00620000087675.
HOGENOMHOG000233630.
HOVERGENHBG004183.
InParanoidQ68FP1.
KOK05768.
OMAMILDTWE.
OrthoDBEOG7288RJ.
PhylomeDBQ68FP1.

Gene expression databases

GenevestigatorQ68FP1.

Family and domain databases

InterProIPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
[Graphical view]
PANTHERPTHR11977. PTHR11977. 1 hit.
PfamPF00626. Gelsolin. 6 hits.
[Graphical view]
PRINTSPR00597. GELSOLIN.
SMARTSM00262. GEL. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio641599.
PROQ68FP1.

Entry information

Entry nameGELS_RAT
AccessionPrimary (citable) accession number: Q68FP1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families