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Protein

Methionine--tRNA ligase, cytoplasmic

Gene

Mars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei598ATPBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • methionine-tRNA ligase activity Source: CAFA
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10By similarity)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:Mars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1345633. Mars.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392631 – 902Methionine--tRNA ligase, cytoplasmicAdd BLAST902

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei827PhosphoserineCombined sources1
Modified residuei837PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ68FL6.
MaxQBiQ68FL6.
PaxDbiQ68FL6.
PeptideAtlasiQ68FL6.
PRIDEiQ68FL6.

PTM databases

iPTMnetiQ68FL6.
PhosphoSitePlusiQ68FL6.
SwissPalmiQ68FL6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040354.
CleanExiMM_MARS.
ExpressionAtlasiQ68FL6. baseline and differential.
GenevisibleiQ68FL6. MM.

Interactioni

Subunit structurei

Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Forms a linear complex that contains MARS, EEF1E1, EPRS and AIMP2 that is at the core of the multisubunit complex.1 Publication

Protein-protein interaction databases

BioGridi229747. 2 interactors.
IntActiQ68FL6. 1 interactor.
STRINGi10090.ENSMUSP00000130666.

Structurei

3D structure databases

ProteinModelPortaliQ68FL6.
SMRiQ68FL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 212GST C-terminalAdd BLAST139
Domaini843 – 899WHEP-TRSAdd BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 285"HIGH" regionAdd BLAST11
Motifi595 – 599"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1247. Eukaryota.
COG0143. LUCA.
GeneTreeiENSGT00550000075017.
HOGENOMiHOG000200402.
HOVERGENiHBG036191.
InParanoidiQ68FL6.
KOiK01874.
PhylomeDBiQ68FL6.
TreeFamiTF300526.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR000738. WHEP-TRS_dom.
PfamiView protein in Pfam
PF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
PRINTSiPR01041. TRNASYNTHMET.
SMARTiView protein in SMART
SM00991. WHEP-TRS. 1 hit.
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q68FL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFVSEGSP GSLPVLAAAA RARGRAELLI STVGPEECVV PFLTRPKVPV
60 70 80 90 100
LQLDSGNYLF SASAICRYFF LLCGWEQDDL TNQWLEWEAT ELQPVLSAAL
110 120 130 140 150
HCLVVQGKKG EDILGPLRRV LTHIDHSLSR QNCPFLAGDT ESLADIVLWG
160 170 180 190 200
ALYPLLQDPA YLPEELGALQ SWFQTLSTQE PCQRAAETVL KQQGVLALRL
210 220 230 240 250
YLQKQPQPQP PPPEGRTVSN ELEEEELATL SEEDIVTAVA AWEKGLESLP
260 270 280 290 300
PLKLQQHPVL PVPGERNVLI TSALPYVNNV PHLGNIIGCV LSADVFARYC
310 320 330 340 350
RLRQWNTLYL CGTDEYGTAT ETKAMEEGLT PREICDKYHA IHADIYRWFG
360 370 380 390 400
ISFDTFGRTT TPQQTKITQD IFQRLLTRGF VLRDTVEQLR CERCARFLAD
410 420 430 440 450
RFVEGVCPFC GYEEARGDQC DRCGKLINAI ELKKPQCKIC RSCPVVRSSQ
460 470 480 490 500
HLFLDLPKLE KRLEDWLGKT VPGSDWTPNA RFIIRSWLRD GLKPRCITRD
510 520 530 540 550
LKWGTPVPLE GFEDKVFYVW FDATIGYVSI TANYTDQWEK WWKNPEQVDL
560 570 580 590 600
YQFMAKDNVP FHGLVFPCSV LGAEDNYTLV KHIIATEYLN YEDGKFSKSR
610 620 630 640 650
GIGVFGDMAK DTGIPADIWR FYLLYIRPEG QDSAFSWTDL LIKNNSELLN
660 670 680 690 700
NLGNFINRAG MFVSKFFGGC VPEMALTPDD RRLVAHVSWE LQHYHQLLEK
710 720 730 740 750
VRIRDALRSI LTISRHGNQY IQVNEPWKRI KGGEMDRQRA GTVTGMAVNM
760 770 780 790 800
AALLSVMLQP YMPTVSSTIQ TQLQLPPAAC RILATSFICT LPAGHRIGTV
810 820 830 840 850
SPLFQKLEND QIENLRQRFG GGQAKGSPKP AAVEAVTAAG SQHIQTLTDE
860 870 880 890 900
VTKQGNVVRE LKAQKADKNQ VAAEVAKLLD LKKQLALAEG KPIETPKGKK

KK
Length:902
Mass (Da):101,431
Last modified:October 11, 2004 - v1
Checksum:iACCE81F1D8049027
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079643 mRNA. Translation: AAH79643.1.
CCDSiCCDS24237.1.
RefSeqiNP_001003913.1. NM_001003913.2.
NP_001165053.1. NM_001171582.1.
UniGeneiMm.28173.

Genome annotation databases

EnsembliENSMUST00000037290; ENSMUSP00000037446; ENSMUSG00000040354.
GeneIDi216443.
KEGGimmu:216443.
UCSCiuc007hiz.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSYMC_MOUSE
AccessioniPrimary (citable) accession number: Q68FL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 11, 2004
Last modified: August 30, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families