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Reviewed, UniProtKB/Swiss-Prot Q68FL6 (SYMC_MOUSE)

Last modified November 3, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS
Gene names
Name: Mars
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length902 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Subunit structure

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43 By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 GST C-terminal domain.

Contains 1 WHEP-TRS domain.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   PTMAcetylation
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrion

Inferred from direct assay. Source: MGI

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 902902Methionyl-tRNA synthetase, cytoplasmic
PRO_0000139263

Regions

Domain74 – 212139GST C-terminal
Domain843 – 89957WHEP-TRS
Motif275 – 28511"HIGH" region
Motif595 – 5995"KMSKS" region

Sites

Binding site5981ATP By similarity

Amino acid modifications

Modified residue7281N6-acetyllysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q68FL6-1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: ACCE81F1D8049027

FASTA902101,431
        10         20         30         40         50         60 
MRLFVSEGSP GSLPVLAAAA RARGRAELLI STVGPEECVV PFLTRPKVPV LQLDSGNYLF 

        70         80         90        100        110        120 
SASAICRYFF LLCGWEQDDL TNQWLEWEAT ELQPVLSAAL HCLVVQGKKG EDILGPLRRV 

       130        140        150        160        170        180 
LTHIDHSLSR QNCPFLAGDT ESLADIVLWG ALYPLLQDPA YLPEELGALQ SWFQTLSTQE 

       190        200        210        220        230        240 
PCQRAAETVL KQQGVLALRL YLQKQPQPQP PPPEGRTVSN ELEEEELATL SEEDIVTAVA 

       250        260        270        280        290        300 
AWEKGLESLP PLKLQQHPVL PVPGERNVLI TSALPYVNNV PHLGNIIGCV LSADVFARYC 

       310        320        330        340        350        360 
RLRQWNTLYL CGTDEYGTAT ETKAMEEGLT PREICDKYHA IHADIYRWFG ISFDTFGRTT 

       370        380        390        400        410        420 
TPQQTKITQD IFQRLLTRGF VLRDTVEQLR CERCARFLAD RFVEGVCPFC GYEEARGDQC 

       430        440        450        460        470        480 
DRCGKLINAI ELKKPQCKIC RSCPVVRSSQ HLFLDLPKLE KRLEDWLGKT VPGSDWTPNA 

       490        500        510        520        530        540 
RFIIRSWLRD GLKPRCITRD LKWGTPVPLE GFEDKVFYVW FDATIGYVSI TANYTDQWEK 

       550        560        570        580        590        600 
WWKNPEQVDL YQFMAKDNVP FHGLVFPCSV LGAEDNYTLV KHIIATEYLN YEDGKFSKSR 

       610        620        630        640        650        660 
GIGVFGDMAK DTGIPADIWR FYLLYIRPEG QDSAFSWTDL LIKNNSELLN NLGNFINRAG 

       670        680        690        700        710        720 
MFVSKFFGGC VPEMALTPDD RRLVAHVSWE LQHYHQLLEK VRIRDALRSI LTISRHGNQY 

       730        740        750        760        770        780 
IQVNEPWKRI KGGEMDRQRA GTVTGMAVNM AALLSVMLQP YMPTVSSTIQ TQLQLPPAAC 

       790        800        810        820        830        840 
RILATSFICT LPAGHRIGTV SPLFQKLEND QIENLRQRFG GGQAKGSPKP AAVEAVTAAG 

       850        860        870        880        890        900 
SQHIQTLTDE VTKQGNVVRE LKAQKADKNQ VAAEVAKLLD LKKQLALAEG KPIETPKGKK 


KK

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References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.

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Cross-references

Sequence databases

BC079643 mRNA. Translation: AAH79643.1.
IPIIPI00461469.
RefSeqNP_001003913.1.
UniGeneMm.28173

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ68FL6.

PTM databases

PhosphoSiteQ68FL6.

Proteomic databases

PRIDEQ68FL6.

Genome annotation databases

EnsemblENSMUST00000037290; ENSMUSP00000037446; ENSMUSG00000040354; Mus musculus. [Genome view]
GeneID216443.
KEGGmmu:216443.
UCSCuc007hiz.1. mouse.

Organism-specific databases

CTD216443.
MGIMGI:1345633. Mars.

Phylogenomic databases

HOVERGENQ68FL6.
OMAYINGIKH.

Enzyme and pathway databases

BRENDA6.1.1.10. 244.

Gene expression databases

ArrayExpressQ68FL6.
BgeeQ68FL6.
CleanExMM_MARS.
GenevestigatorQ68FL6.
GermOnlineENSMUSG00000040354. Mus musculus.

Family and domain databases

InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR002304. Met-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA_bd.
IPR014758. tRNA-synt_met_N.
IPR000738. WHEP-TRS.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.
PfamPF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio375163.
SOURCESearch...

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Entry information

Entry nameSYMC_MOUSE
AccessionPrimary (citable) accession number: Q68FL6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 11, 2004
Last modified: November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents