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Protein

Methionine--tRNA ligase, cytoplasmic

Gene

Mars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei598ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:Mars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1345633. Mars.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: GO_Central
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • nucleolus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392631 – 902Methionine--tRNA ligase, cytoplasmicAdd BLAST902

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei827PhosphoserineCombined sources1
Modified residuei837PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ68FL6.
MaxQBiQ68FL6.
PaxDbiQ68FL6.
PeptideAtlasiQ68FL6.
PRIDEiQ68FL6.

PTM databases

iPTMnetiQ68FL6.
PhosphoSitePlusiQ68FL6.
SwissPalmiQ68FL6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040354.
CleanExiMM_MARS.
ExpressionAtlasiQ68FL6. baseline and differential.
GenevisibleiQ68FL6. MM.

Interactioni

Subunit structurei

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.By similarity

Protein-protein interaction databases

BioGridi229747. 2 interactors.
IntActiQ68FL6. 1 interactor.
STRINGi10090.ENSMUSP00000130666.

Structurei

3D structure databases

ProteinModelPortaliQ68FL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 212GST C-terminalAdd BLAST139
Domaini843 – 899WHEP-TRSAdd BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 285"HIGH" regionAdd BLAST11
Motifi595 – 599"KMSKS" region5

Sequence similaritiesi

Contains 1 GST C-terminal domain.Curated
Contains 1 WHEP-TRS domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1247. Eukaryota.
COG0143. LUCA.
GeneTreeiENSGT00550000075017.
HOGENOMiHOG000200402.
HOVERGENiHBG036191.
InParanoidiQ68FL6.
KOiK01874.
PhylomeDBiQ68FL6.
TreeFamiTF300526.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.287.10. 1 hit.
1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q68FL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFVSEGSP GSLPVLAAAA RARGRAELLI STVGPEECVV PFLTRPKVPV
60 70 80 90 100
LQLDSGNYLF SASAICRYFF LLCGWEQDDL TNQWLEWEAT ELQPVLSAAL
110 120 130 140 150
HCLVVQGKKG EDILGPLRRV LTHIDHSLSR QNCPFLAGDT ESLADIVLWG
160 170 180 190 200
ALYPLLQDPA YLPEELGALQ SWFQTLSTQE PCQRAAETVL KQQGVLALRL
210 220 230 240 250
YLQKQPQPQP PPPEGRTVSN ELEEEELATL SEEDIVTAVA AWEKGLESLP
260 270 280 290 300
PLKLQQHPVL PVPGERNVLI TSALPYVNNV PHLGNIIGCV LSADVFARYC
310 320 330 340 350
RLRQWNTLYL CGTDEYGTAT ETKAMEEGLT PREICDKYHA IHADIYRWFG
360 370 380 390 400
ISFDTFGRTT TPQQTKITQD IFQRLLTRGF VLRDTVEQLR CERCARFLAD
410 420 430 440 450
RFVEGVCPFC GYEEARGDQC DRCGKLINAI ELKKPQCKIC RSCPVVRSSQ
460 470 480 490 500
HLFLDLPKLE KRLEDWLGKT VPGSDWTPNA RFIIRSWLRD GLKPRCITRD
510 520 530 540 550
LKWGTPVPLE GFEDKVFYVW FDATIGYVSI TANYTDQWEK WWKNPEQVDL
560 570 580 590 600
YQFMAKDNVP FHGLVFPCSV LGAEDNYTLV KHIIATEYLN YEDGKFSKSR
610 620 630 640 650
GIGVFGDMAK DTGIPADIWR FYLLYIRPEG QDSAFSWTDL LIKNNSELLN
660 670 680 690 700
NLGNFINRAG MFVSKFFGGC VPEMALTPDD RRLVAHVSWE LQHYHQLLEK
710 720 730 740 750
VRIRDALRSI LTISRHGNQY IQVNEPWKRI KGGEMDRQRA GTVTGMAVNM
760 770 780 790 800
AALLSVMLQP YMPTVSSTIQ TQLQLPPAAC RILATSFICT LPAGHRIGTV
810 820 830 840 850
SPLFQKLEND QIENLRQRFG GGQAKGSPKP AAVEAVTAAG SQHIQTLTDE
860 870 880 890 900
VTKQGNVVRE LKAQKADKNQ VAAEVAKLLD LKKQLALAEG KPIETPKGKK

KK
Length:902
Mass (Da):101,431
Last modified:October 11, 2004 - v1
Checksum:iACCE81F1D8049027
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079643 mRNA. Translation: AAH79643.1.
CCDSiCCDS24237.1.
RefSeqiNP_001003913.1. NM_001003913.2.
NP_001165053.1. NM_001171582.1.
UniGeneiMm.28173.

Genome annotation databases

EnsembliENSMUST00000037290; ENSMUSP00000037446; ENSMUSG00000040354.
GeneIDi216443.
KEGGimmu:216443.
UCSCiuc007hiz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079643 mRNA. Translation: AAH79643.1.
CCDSiCCDS24237.1.
RefSeqiNP_001003913.1. NM_001003913.2.
NP_001165053.1. NM_001171582.1.
UniGeneiMm.28173.

3D structure databases

ProteinModelPortaliQ68FL6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229747. 2 interactors.
IntActiQ68FL6. 1 interactor.
STRINGi10090.ENSMUSP00000130666.

PTM databases

iPTMnetiQ68FL6.
PhosphoSitePlusiQ68FL6.
SwissPalmiQ68FL6.

Proteomic databases

EPDiQ68FL6.
MaxQBiQ68FL6.
PaxDbiQ68FL6.
PeptideAtlasiQ68FL6.
PRIDEiQ68FL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037290; ENSMUSP00000037446; ENSMUSG00000040354.
GeneIDi216443.
KEGGimmu:216443.
UCSCiuc007hiz.2. mouse.

Organism-specific databases

CTDi4141.
MGIiMGI:1345633. Mars.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1247. Eukaryota.
COG0143. LUCA.
GeneTreeiENSGT00550000075017.
HOGENOMiHOG000200402.
HOVERGENiHBG036191.
InParanoidiQ68FL6.
KOiK01874.
PhylomeDBiQ68FL6.
TreeFamiTF300526.

Miscellaneous databases

ChiTaRSiMars. mouse.
PROiQ68FL6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040354.
CleanExiMM_MARS.
ExpressionAtlasiQ68FL6. baseline and differential.
GenevisibleiQ68FL6. MM.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.287.10. 1 hit.
1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYMC_MOUSE
AccessioniPrimary (citable) accession number: Q68FL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.