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Q68FH0 (PKP4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plakophilin-4
Alternative name(s):
Armadillo-related protein
Gene names
Name:Pkp4
Synonyms:Armrp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1190 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques By similarity.

Subunit structure

Interacts (via the C-terminus) with FRMPD2 (via the PDZ 2 domain). Interacts with PDZD2. Interacts with RHOA; the interaction is detected at the midbody. Interacts with ECT2; the interaction is detected at the midbody By similarity.

Subcellular location

Cell junctiondesmosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Cytoplasmcytoskeletonspindle pole By similarity. Midbody By similarity. Cell membrane; Peripheral membrane protein By similarity. Note: Associated with the pericentrosomal region in interphase and with spindle poles during mitosis. In anaphase, during chromosome segregation, is recruited to the central microtubule bundle, focussed at the spindle midzone and ultimately localizes to the midbody at cytokinesis. Constituent of the midbody cytoskeletal matrix. Colocalized with desmoplakin at desmosomal junctional plaques in cultured epithelial cells By similarity.

Sequence similarities

Belongs to the beta-catenin family.

Contains 4 ARM repeats.

Sequence caution

The sequence BAC37187.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell junction
Cell membrane
Cytoplasm
Cytoskeleton
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell-cell junction assembly

Inferred from mutant phenotype PubMed 22965878. Source: UniProtKB

positive regulation of Rho GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cytokinesis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of gene expression

Inferred from mutant phenotype PubMed 22965878. Source: UniProtKB

single organismal cell-cell adhesion

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcell-cell contact zone

Inferred from electronic annotation. Source: Ensembl

cell-cell junction

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasmic side of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

cytoskeleton

Inferred from sequence or structural similarity. Source: UniProtKB

desmosome

Inferred from sequence or structural similarity. Source: UniProtKB

midbody

Inferred from sequence or structural similarity. Source: UniProtKB

mitotic spindle

Inferred from sequence or structural similarity. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

spindle midzone

Inferred from sequence or structural similarity. Source: UniProtKB

spindle pole

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q68FH0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q68FH0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1042-1084: Missing.
Isoform 3 (identifier: Q68FH0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     637-637: V → G
     638-1190: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11901190Plakophilin-4
PRO_0000064290

Regions

Repeat517 – 55640ARM 1
Repeat559 – 59840ARM 2
Repeat603 – 64341ARM 3
Repeat861 – 90040ARM 4
Coiled coil36 – 6328 Potential
Compositional bias787 – 7937Poly-Lys

Amino acid modifications

Modified residue2201Phosphoserine By similarity
Modified residue2301Phosphoserine By similarity
Modified residue2721Phosphoserine By similarity
Modified residue2801Phosphoserine By similarity
Modified residue3131Phosphoserine Ref.4
Modified residue3261Phosphoserine By similarity
Modified residue3711Phosphotyrosine Ref.6
Modified residue4021Phosphoserine By similarity
Modified residue4051Phosphoserine Ref.4
Modified residue4141Phosphotyrosine By similarity
Modified residue4771Phosphotyrosine Ref.6
Modified residue7751Phosphoserine By similarity
Modified residue11331Phosphoserine Ref.4
Cross-link610Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence1 – 341341Missing in isoform 3.
VSP_012374
Alternative sequence6371V → G in isoform 3.
VSP_012375
Alternative sequence638 – 1190553Missing in isoform 3.
VSP_012376
Alternative sequence1042 – 108443Missing in isoform 2.
VSP_012377

Experimental info

Sequence conflict3281S → F in AAH78638. Ref.2
Sequence conflict8771V → A in BAC36708. Ref.3
Sequence conflict11181D → G in BAC36708. Ref.3
Sequence conflict11611K → R in BAB32313. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: DB6548B5EBE72CFE

FASTA1,190131,551
        10         20         30         40         50         60 
MPAPEQGSLV EEGQPQTHQE AVSTGPGMEP ETTATTILAS VKEQELQFQR LTRELEVERQ 

        70         80         90        100        110        120 
IVASQLERCR LGAESPSIAS TSSTEKSFPW RSTDVPNPGV SKPRVSDTIH PNNYLIRTEP 

       130        140        150        160        170        180 
EQGTLYSPEQ TSLHESEGSL GNSRSSTQMN SYSDSGYQEA GSFHNSQTVN KADSRQHPFT 

       190        200        210        220        230        240 
GSTSNHVVRT SRAEGQTLVQ PSVANRAMRR VSSVPSRAQS PSYVTSTGVS PSRGSLRTSL 

       250        260        270        280        290        300 
GSGFGSPSVT DSRPLNPSAY SSSTLPAQRA ASPYSQRPAS PTAVRRVGSV TSRQTSNPNG 

       310        320        330        340        350        360 
PVPQYQTTTR VGSPLTLTDA QTRVASPSQG QVGSSSPKRS GMTAVPQHLG PSLQRTVHDM 

       370        380        390        400        410        420 
DQFGQQQYDI YERMVPPRPD SLTGLRSSYA SQHSQLGQEL RSAVSPDLHI TPIYEGRTYY 

       430        440        450        460        470        480 
SPVYRSPNHG TVELQGSQTA LYRTGSVGIG NLQRTSSQRS TLTYQRNNYA LNTAATYAEP 

       490        500        510        520        530        540 
YRPVQYRVQE CSYNRLQHTG PADDGATRSP SIDSIQKDPR EFAWRDPELP EVIHMLQHQF 

       550        560        570        580        590        600 
PSVQANAAAY LQHLCFGDNK VKMEVYRLGG IKHLVDLLDH RVLEVQKNAC GALRNLVFGK 

       610        620        630        640        650        660 
STDENKIAMK NVGGIPALLR LLRKSIDAEV RELVTGVLWN LSSCDAVKMT IIRDALSTLT 

       670        680        690        700        710        720 
NTVIVPHSGW NNSSFDDDHK IKFQTSLVLR NTTGCLRNLS SAGEEARKQM RSCEGLVDSL 

       730        740        750        760        770        780 
LYVIHTCVNT SDYDSKTVEN CVCTLRNLSY RLELEVPQAR LLGLNELDDL LGKESPSKDS 

       790        800        810        820        830        840 
EPSCWGKKKK KKKRTPQEDQ WDGVGPIPGL SKSPKGVEML WHPSVVKPYL TLLAESSNPA 

       850        860        870        880        890        900 
TLEGSAGSLQ NLSAGNWKFA AYIRAAVRKE KGLPILVELL RMDNDRVVSS VATALRNMAL 

       910        920        930        940        950        960 
DVRNKELIGK YAMRDLVNRL PGGNGPSILS DETVAAICCA LHEVTSKNME NAKALADSGG 

       970        980        990       1000       1010       1020 
IEKLVNITKG RGDRSSLKVV KAAAQVLNTL WQYRDLRSIY KKDGWNQNHF ITPVSTLERD 

      1030       1040       1050       1060       1070       1080 
RFKSHPSLST TNQQMSPIIQ SVGSTSSSPA LLGIREPRSE YDRTQPPMQY YNSQGDTTHK 

      1090       1100       1110       1120       1130       1140 
GLYPGSSKPS PIYISSYSSP AREQNRRLQH QQLYYQDDST RKTLDAYRLY LQSPRSYEDP 

      1150       1160       1170       1180       1190 
YCDDRVHFPA STDYSTQYGL KSTTNYVDFY STKRPSYRAE QYPGSPDSWV 

« Hide

Isoform 2 [UniParc].

Checksum: DEC21F1C1F960B7A
Show »

FASTA1,147126,783
Isoform 3 [UniParc].

Checksum: D0630AA63A0911E8
Show »

FASTA29633,263

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and Czech II.
Tissue: Brain, Eye and Mammary cancer.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 747-1190 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Olfactory bulb, Ovary and Uterus.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313; SER-405 AND SER-1133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[5]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
[6]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-371 AND TYR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL845536 Genomic DNA. Translation: CAM20481.1.
BC078638 mRNA. Translation: AAH78638.1.
BC079848 mRNA. Translation: AAH79848.1.
BC082578 mRNA. Translation: AAH82578.1.
AK021168 mRNA. Translation: BAB32313.1.
AK077250 mRNA. Translation: BAC36708.1.
AK078240 mRNA. Translation: BAC37187.1. Different initiation.
CCDSCCDS16052.1. [Q68FH0-1]
CCDS50588.1. [Q68FH0-2]
RefSeqNP_080637.1. NM_026361.2. [Q68FH0-1]
NP_780673.2. NM_175464.2. [Q68FH0-2]
XP_006499234.1. XM_006499171.1. [Q68FH0-1]
UniGeneMm.260938.

3D structure databases

ProteinModelPortalQ68FH0.
SMRQ68FH0. Positions 524-1010.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid230696. 3 interactions.

PTM databases

PhosphoSiteQ68FH0.

Proteomic databases

MaxQBQ68FH0.
PaxDbQ68FH0.
PRIDEQ68FH0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102754; ENSMUSP00000099815; ENSMUSG00000026991. [Q68FH0-1]
ENSMUST00000168631; ENSMUSP00000129836; ENSMUSG00000026991. [Q68FH0-2]
GeneID227937.
KEGGmmu:227937.
UCSCuc008jtb.1. mouse. [Q68FH0-1]
uc008jtd.1. mouse. [Q68FH0-2]
uc008jtg.1. mouse. [Q68FH0-3]

Organism-specific databases

CTD8502.
MGIMGI:109281. Pkp4.

Phylogenomic databases

eggNOGNOG276924.
GeneTreeENSGT00690000101851.
HOVERGENHBG004284.
InParanoidA2AS46.
OMAGIGNLQR.
OrthoDBEOG7X6KZB.
PhylomeDBQ68FH0.
TreeFamTF321877.

Gene expression databases

ArrayExpressQ68FH0.
BgeeQ68FH0.
CleanExMM_PKP4.
GenevestigatorQ68FH0.

Family and domain databases

Gene3D1.25.10.10. 2 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028443. Plakophilin-4.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERPTHR10372. PTHR10372. 1 hit.
PTHR10372:SF8. PTHR10372:SF8. 1 hit.
PfamPF00514. Arm. 3 hits.
[Graphical view]
SMARTSM00185. ARM. 6 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50176. ARM_REPEAT. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPKP4. mouse.
NextBio378870.
PROQ68FH0.
SOURCESearch...

Entry information

Entry namePKP4_MOUSE
AccessionPrimary (citable) accession number: Q68FH0
Secondary accession number(s): A2AS46 expand/collapse secondary AC list , Q640N0, Q68G56, Q8BK47, Q8BVH1, Q9CRE3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot