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Protein

Plakophilin-4

Gene

Pkp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Plakophilin-4
Alternative name(s):
Armadillo-related protein
Gene namesi
Name:Pkp4
Synonyms:Armrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109281. Pkp4.

Subcellular locationi

  • Cell junctiondesmosome By similarity
  • Cytoplasmcytoskeletonspindle By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity
  • Midbody By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity

  • Note: Associated with the pericentrosomal region in interphase and with spindle poles during mitosis. In anaphase, during chromosome segregation, is recruited to the central microtubule bundle, focussed at the spindle midzone and ultimately localizes to the midbody at cytokinesis. Constituent of the midbody cytoskeletal matrix. Colocalized with desmoplakin at desmosomal junctional plaques in cultured epithelial cells (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000642901 – 1190Plakophilin-4Add BLAST1190

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei75PhosphoserineBy similarity1
Modified residuei84PhosphothreonineCombined sources1
Modified residuei106PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei220PhosphoserineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei235PhosphoserineBy similarity1
Modified residuei253Omega-N-methylarginineCombined sources1
Modified residuei269Omega-N-methylarginineCombined sources1
Modified residuei272PhosphoserineBy similarity1
Modified residuei280PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei336PhosphoserineCombined sources1
Modified residuei371PhosphotyrosineCombined sources1
Modified residuei391PhosphoserineBy similarity1
Modified residuei402PhosphoserineCombined sources1
Modified residuei405PhosphoserineCombined sources1
Modified residuei411PhosphothreonineCombined sources1
Modified residuei414PhosphotyrosineBy similarity1
Modified residuei421PhosphoserineCombined sources1
Modified residuei426PhosphoserineCombined sources1
Modified residuei437PhosphoserineCombined sources1
Modified residuei477PhosphotyrosineCombined sources1
Modified residuei509PhosphoserineCombined sources1
Modified residuei511PhosphoserineCombined sources1
Modified residuei514PhosphoserineCombined sources1
Modified residuei775PhosphoserineCombined sources1
Modified residuei1012PhosphothreonineCombined sources1
Modified residuei1016PhosphothreonineCombined sources1
Modified residuei1044PhosphoserineCombined sources1
Modified residuei1090PhosphoserineCombined sources1
Modified residuei1099PhosphoserineCombined sources1
Modified residuei1133PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ68FH0.
PeptideAtlasiQ68FH0.
PRIDEiQ68FH0.

PTM databases

iPTMnetiQ68FH0.
PhosphoSitePlusiQ68FH0.
SwissPalmiQ68FH0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026991.
CleanExiMM_PKP4.
ExpressionAtlasiQ68FH0. baseline and differential.
GenevisibleiQ68FH0. MM.

Interactioni

Subunit structurei

Interacts (via the C-terminus) with FRMPD2 (via the PDZ 2 domain). Interacts with PDZD2. Interacts with RHOA; the interaction is detected at the midbody. Interacts with ECT2; the interaction is detected at the midbody (By similarity).By similarity

Protein-protein interaction databases

BioGridi230696. 3 interactors.
STRINGi10090.ENSMUSP00000099815.

Structurei

3D structure databases

ProteinModelPortaliQ68FH0.
SMRiQ68FH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati517 – 556ARM 1Add BLAST40
Repeati559 – 598ARM 2Add BLAST40
Repeati603 – 643ARM 3Add BLAST41
Repeati861 – 900ARM 4Add BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 63Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi787 – 793Poly-Lys7

Sequence similaritiesi

Belongs to the beta-catenin family.Curated
Contains 4 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1048. Eukaryota.
ENOG410Y21Q. LUCA.
GeneTreeiENSGT00760000119167.
HOVERGENiHBG004284.
InParanoidiQ68FH0.
OMAiFHNSQNV.
OrthoDBiEOG091G00SB.
PhylomeDBiQ68FH0.
TreeFamiTF321877.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028443. Plakophilin-4.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERiPTHR10372. PTHR10372. 2 hits.
PTHR10372:SF8. PTHR10372:SF8. 2 hits.
PfamiPF00514. Arm. 3 hits.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68FH0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAPEQGSLV EEGQPQTHQE AVSTGPGMEP ETTATTILAS VKEQELQFQR
60 70 80 90 100
LTRELEVERQ IVASQLERCR LGAESPSIAS TSSTEKSFPW RSTDVPNPGV
110 120 130 140 150
SKPRVSDTIH PNNYLIRTEP EQGTLYSPEQ TSLHESEGSL GNSRSSTQMN
160 170 180 190 200
SYSDSGYQEA GSFHNSQTVN KADSRQHPFT GSTSNHVVRT SRAEGQTLVQ
210 220 230 240 250
PSVANRAMRR VSSVPSRAQS PSYVTSTGVS PSRGSLRTSL GSGFGSPSVT
260 270 280 290 300
DSRPLNPSAY SSSTLPAQRA ASPYSQRPAS PTAVRRVGSV TSRQTSNPNG
310 320 330 340 350
PVPQYQTTTR VGSPLTLTDA QTRVASPSQG QVGSSSPKRS GMTAVPQHLG
360 370 380 390 400
PSLQRTVHDM DQFGQQQYDI YERMVPPRPD SLTGLRSSYA SQHSQLGQEL
410 420 430 440 450
RSAVSPDLHI TPIYEGRTYY SPVYRSPNHG TVELQGSQTA LYRTGSVGIG
460 470 480 490 500
NLQRTSSQRS TLTYQRNNYA LNTAATYAEP YRPVQYRVQE CSYNRLQHTG
510 520 530 540 550
PADDGATRSP SIDSIQKDPR EFAWRDPELP EVIHMLQHQF PSVQANAAAY
560 570 580 590 600
LQHLCFGDNK VKMEVYRLGG IKHLVDLLDH RVLEVQKNAC GALRNLVFGK
610 620 630 640 650
STDENKIAMK NVGGIPALLR LLRKSIDAEV RELVTGVLWN LSSCDAVKMT
660 670 680 690 700
IIRDALSTLT NTVIVPHSGW NNSSFDDDHK IKFQTSLVLR NTTGCLRNLS
710 720 730 740 750
SAGEEARKQM RSCEGLVDSL LYVIHTCVNT SDYDSKTVEN CVCTLRNLSY
760 770 780 790 800
RLELEVPQAR LLGLNELDDL LGKESPSKDS EPSCWGKKKK KKKRTPQEDQ
810 820 830 840 850
WDGVGPIPGL SKSPKGVEML WHPSVVKPYL TLLAESSNPA TLEGSAGSLQ
860 870 880 890 900
NLSAGNWKFA AYIRAAVRKE KGLPILVELL RMDNDRVVSS VATALRNMAL
910 920 930 940 950
DVRNKELIGK YAMRDLVNRL PGGNGPSILS DETVAAICCA LHEVTSKNME
960 970 980 990 1000
NAKALADSGG IEKLVNITKG RGDRSSLKVV KAAAQVLNTL WQYRDLRSIY
1010 1020 1030 1040 1050
KKDGWNQNHF ITPVSTLERD RFKSHPSLST TNQQMSPIIQ SVGSTSSSPA
1060 1070 1080 1090 1100
LLGIREPRSE YDRTQPPMQY YNSQGDTTHK GLYPGSSKPS PIYISSYSSP
1110 1120 1130 1140 1150
AREQNRRLQH QQLYYQDDST RKTLDAYRLY LQSPRSYEDP YCDDRVHFPA
1160 1170 1180 1190
STDYSTQYGL KSTTNYVDFY STKRPSYRAE QYPGSPDSWV
Length:1,190
Mass (Da):131,551
Last modified:October 11, 2004 - v1
Checksum:iDB6548B5EBE72CFE
GO
Isoform 2 (identifier: Q68FH0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1042-1084: Missing.

Show »
Length:1,147
Mass (Da):126,783
Checksum:iDEC21F1C1F960B7A
GO
Isoform 3 (identifier: Q68FH0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     637-637: V → G
     638-1190: Missing.

Show »
Length:296
Mass (Da):33,263
Checksum:iD0630AA63A0911E8
GO

Sequence cautioni

The sequence BAC37187 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti328S → F in AAH78638 (PubMed:15489334).Curated1
Sequence conflicti877V → A in BAC36708 (PubMed:16141072).Curated1
Sequence conflicti1118D → G in BAC36708 (PubMed:16141072).Curated1
Sequence conflicti1161K → R in BAB32313 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0123741 – 341Missing in isoform 3. 1 PublicationAdd BLAST341
Alternative sequenceiVSP_012375637V → G in isoform 3. 1 Publication1
Alternative sequenceiVSP_012376638 – 1190Missing in isoform 3. 1 PublicationAdd BLAST553
Alternative sequenceiVSP_0123771042 – 1084Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845536 Genomic DNA. Translation: CAM20481.1.
BC078638 mRNA. Translation: AAH78638.1.
BC079848 mRNA. Translation: AAH79848.1.
BC082578 mRNA. Translation: AAH82578.1.
AK021168 mRNA. Translation: BAB32313.1.
AK077250 mRNA. Translation: BAC36708.1.
AK078240 mRNA. Translation: BAC37187.1. Different initiation.
CCDSiCCDS16052.1. [Q68FH0-1]
CCDS50588.1. [Q68FH0-2]
RefSeqiNP_080637.1. NM_026361.2. [Q68FH0-1]
NP_780673.2. NM_175464.2. [Q68FH0-2]
XP_006499234.1. XM_006499171.3. [Q68FH0-1]
UniGeneiMm.260938.

Genome annotation databases

EnsembliENSMUST00000102754; ENSMUSP00000099815; ENSMUSG00000026991. [Q68FH0-1]
ENSMUST00000168631; ENSMUSP00000129836; ENSMUSG00000026991. [Q68FH0-2]
GeneIDi227937.
KEGGimmu:227937.
UCSCiuc008jtb.1. mouse. [Q68FH0-1]
uc008jtd.1. mouse. [Q68FH0-2]
uc008jtg.1. mouse. [Q68FH0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845536 Genomic DNA. Translation: CAM20481.1.
BC078638 mRNA. Translation: AAH78638.1.
BC079848 mRNA. Translation: AAH79848.1.
BC082578 mRNA. Translation: AAH82578.1.
AK021168 mRNA. Translation: BAB32313.1.
AK077250 mRNA. Translation: BAC36708.1.
AK078240 mRNA. Translation: BAC37187.1. Different initiation.
CCDSiCCDS16052.1. [Q68FH0-1]
CCDS50588.1. [Q68FH0-2]
RefSeqiNP_080637.1. NM_026361.2. [Q68FH0-1]
NP_780673.2. NM_175464.2. [Q68FH0-2]
XP_006499234.1. XM_006499171.3. [Q68FH0-1]
UniGeneiMm.260938.

3D structure databases

ProteinModelPortaliQ68FH0.
SMRiQ68FH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230696. 3 interactors.
STRINGi10090.ENSMUSP00000099815.

PTM databases

iPTMnetiQ68FH0.
PhosphoSitePlusiQ68FH0.
SwissPalmiQ68FH0.

Proteomic databases

PaxDbiQ68FH0.
PeptideAtlasiQ68FH0.
PRIDEiQ68FH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102754; ENSMUSP00000099815; ENSMUSG00000026991. [Q68FH0-1]
ENSMUST00000168631; ENSMUSP00000129836; ENSMUSG00000026991. [Q68FH0-2]
GeneIDi227937.
KEGGimmu:227937.
UCSCiuc008jtb.1. mouse. [Q68FH0-1]
uc008jtd.1. mouse. [Q68FH0-2]
uc008jtg.1. mouse. [Q68FH0-3]

Organism-specific databases

CTDi8502.
MGIiMGI:109281. Pkp4.

Phylogenomic databases

eggNOGiKOG1048. Eukaryota.
ENOG410Y21Q. LUCA.
GeneTreeiENSGT00760000119167.
HOVERGENiHBG004284.
InParanoidiQ68FH0.
OMAiFHNSQNV.
OrthoDBiEOG091G00SB.
PhylomeDBiQ68FH0.
TreeFamiTF321877.

Enzyme and pathway databases

ReactomeiR-MMU-6805567. Keratinization.
R-MMU-6809371. Formation of the cornified envelope.

Miscellaneous databases

ChiTaRSiPkp4. mouse.
PROiQ68FH0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026991.
CleanExiMM_PKP4.
ExpressionAtlasiQ68FH0. baseline and differential.
GenevisibleiQ68FH0. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028443. Plakophilin-4.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERiPTHR10372. PTHR10372. 2 hits.
PTHR10372:SF8. PTHR10372:SF8. 2 hits.
PfamiPF00514. Arm. 3 hits.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKP4_MOUSE
AccessioniPrimary (citable) accession number: Q68FH0
Secondary accession number(s): A2AS46
, Q640N0, Q68G56, Q8BK47, Q8BVH1, Q9CRE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.