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Protein

Plakophilin-4

Gene

Pkp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as a regulator of Rho activity during cytokinesis. May play a role in junctional plaques (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Plakophilin-4
Alternative name(s):
Armadillo-related protein
Gene namesi
Name:Pkp4
Synonyms:Armrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109281. Pkp4.

Subcellular locationi

  • Cell junctiondesmosome By similarity
  • Cytoplasmcytoskeletonspindle By similarity
  • Cytoplasmcytoskeletonspindle pole By similarity
  • Midbody By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity

  • Note: Associated with the pericentrosomal region in interphase and with spindle poles during mitosis. In anaphase, during chromosome segregation, is recruited to the central microtubule bundle, focussed at the spindle midzone and ultimately localizes to the midbody at cytokinesis. Constituent of the midbody cytoskeletal matrix. Colocalized with desmoplakin at desmosomal junctional plaques in cultured epithelial cells (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11901190Plakophilin-4PRO_0000064290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei75 – 751PhosphoserineBy similarity
Modified residuei84 – 841PhosphothreonineCombined sources
Modified residuei106 – 1061PhosphoserineBy similarity
Modified residuei132 – 1321PhosphoserineCombined sources
Modified residuei136 – 1361PhosphoserineCombined sources
Modified residuei139 – 1391PhosphoserineCombined sources
Modified residuei220 – 2201PhosphoserineCombined sources
Modified residuei230 – 2301PhosphoserineCombined sources
Modified residuei235 – 2351PhosphoserineBy similarity
Modified residuei272 – 2721PhosphoserineBy similarity
Modified residuei280 – 2801PhosphoserineCombined sources
Modified residuei313 – 3131PhosphoserineCombined sources
Modified residuei326 – 3261PhosphoserineCombined sources
Modified residuei336 – 3361PhosphoserineCombined sources
Modified residuei371 – 3711PhosphotyrosineCombined sources
Modified residuei391 – 3911PhosphoserineBy similarity
Modified residuei402 – 4021PhosphoserineCombined sources
Modified residuei405 – 4051PhosphoserineCombined sources
Modified residuei411 – 4111PhosphothreonineCombined sources
Modified residuei414 – 4141PhosphotyrosineBy similarity
Modified residuei421 – 4211PhosphoserineCombined sources
Modified residuei426 – 4261PhosphoserineCombined sources
Modified residuei437 – 4371PhosphoserineCombined sources
Modified residuei477 – 4771PhosphotyrosineCombined sources
Modified residuei509 – 5091PhosphoserineCombined sources
Modified residuei511 – 5111PhosphoserineCombined sources
Modified residuei514 – 5141PhosphoserineCombined sources
Cross-linki610 – 610Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei775 – 7751PhosphoserineCombined sources
Modified residuei1012 – 10121PhosphothreonineCombined sources
Modified residuei1016 – 10161PhosphothreonineCombined sources
Modified residuei1044 – 10441PhosphoserineCombined sources
Modified residuei1090 – 10901PhosphoserineCombined sources
Modified residuei1099 – 10991PhosphoserineCombined sources
Modified residuei1133 – 11331PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ68FH0.
PaxDbiQ68FH0.
PeptideAtlasiQ68FH0.
PRIDEiQ68FH0.

PTM databases

iPTMnetiQ68FH0.
PhosphoSiteiQ68FH0.
SwissPalmiQ68FH0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026991.
CleanExiMM_PKP4.
ExpressionAtlasiQ68FH0. baseline and differential.
GenevisibleiQ68FH0. MM.

Interactioni

Subunit structurei

Interacts (via the C-terminus) with FRMPD2 (via the PDZ 2 domain). Interacts with PDZD2. Interacts with RHOA; the interaction is detected at the midbody. Interacts with ECT2; the interaction is detected at the midbody (By similarity).By similarity

Protein-protein interaction databases

BioGridi230696. 3 interactions.
STRINGi10090.ENSMUSP00000099815.

Structurei

3D structure databases

ProteinModelPortaliQ68FH0.
SMRiQ68FH0. Positions 524-1010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati517 – 55640ARM 1Add
BLAST
Repeati559 – 59840ARM 2Add
BLAST
Repeati603 – 64341ARM 3Add
BLAST
Repeati861 – 90040ARM 4Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili36 – 6328Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi787 – 7937Poly-Lys

Sequence similaritiesi

Belongs to the beta-catenin family.Curated
Contains 4 ARM repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1048. Eukaryota.
ENOG410Y21Q. LUCA.
GeneTreeiENSGT00760000119167.
HOVERGENiHBG004284.
InParanoidiQ68FH0.
OMAiFHNSQNV.
OrthoDBiEOG091G00SB.
PhylomeDBiQ68FH0.
TreeFamiTF321877.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028443. Plakophilin-4.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERiPTHR10372. PTHR10372. 2 hits.
PTHR10372:SF8. PTHR10372:SF8. 2 hits.
PfamiPF00514. Arm. 3 hits.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68FH0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAPEQGSLV EEGQPQTHQE AVSTGPGMEP ETTATTILAS VKEQELQFQR
60 70 80 90 100
LTRELEVERQ IVASQLERCR LGAESPSIAS TSSTEKSFPW RSTDVPNPGV
110 120 130 140 150
SKPRVSDTIH PNNYLIRTEP EQGTLYSPEQ TSLHESEGSL GNSRSSTQMN
160 170 180 190 200
SYSDSGYQEA GSFHNSQTVN KADSRQHPFT GSTSNHVVRT SRAEGQTLVQ
210 220 230 240 250
PSVANRAMRR VSSVPSRAQS PSYVTSTGVS PSRGSLRTSL GSGFGSPSVT
260 270 280 290 300
DSRPLNPSAY SSSTLPAQRA ASPYSQRPAS PTAVRRVGSV TSRQTSNPNG
310 320 330 340 350
PVPQYQTTTR VGSPLTLTDA QTRVASPSQG QVGSSSPKRS GMTAVPQHLG
360 370 380 390 400
PSLQRTVHDM DQFGQQQYDI YERMVPPRPD SLTGLRSSYA SQHSQLGQEL
410 420 430 440 450
RSAVSPDLHI TPIYEGRTYY SPVYRSPNHG TVELQGSQTA LYRTGSVGIG
460 470 480 490 500
NLQRTSSQRS TLTYQRNNYA LNTAATYAEP YRPVQYRVQE CSYNRLQHTG
510 520 530 540 550
PADDGATRSP SIDSIQKDPR EFAWRDPELP EVIHMLQHQF PSVQANAAAY
560 570 580 590 600
LQHLCFGDNK VKMEVYRLGG IKHLVDLLDH RVLEVQKNAC GALRNLVFGK
610 620 630 640 650
STDENKIAMK NVGGIPALLR LLRKSIDAEV RELVTGVLWN LSSCDAVKMT
660 670 680 690 700
IIRDALSTLT NTVIVPHSGW NNSSFDDDHK IKFQTSLVLR NTTGCLRNLS
710 720 730 740 750
SAGEEARKQM RSCEGLVDSL LYVIHTCVNT SDYDSKTVEN CVCTLRNLSY
760 770 780 790 800
RLELEVPQAR LLGLNELDDL LGKESPSKDS EPSCWGKKKK KKKRTPQEDQ
810 820 830 840 850
WDGVGPIPGL SKSPKGVEML WHPSVVKPYL TLLAESSNPA TLEGSAGSLQ
860 870 880 890 900
NLSAGNWKFA AYIRAAVRKE KGLPILVELL RMDNDRVVSS VATALRNMAL
910 920 930 940 950
DVRNKELIGK YAMRDLVNRL PGGNGPSILS DETVAAICCA LHEVTSKNME
960 970 980 990 1000
NAKALADSGG IEKLVNITKG RGDRSSLKVV KAAAQVLNTL WQYRDLRSIY
1010 1020 1030 1040 1050
KKDGWNQNHF ITPVSTLERD RFKSHPSLST TNQQMSPIIQ SVGSTSSSPA
1060 1070 1080 1090 1100
LLGIREPRSE YDRTQPPMQY YNSQGDTTHK GLYPGSSKPS PIYISSYSSP
1110 1120 1130 1140 1150
AREQNRRLQH QQLYYQDDST RKTLDAYRLY LQSPRSYEDP YCDDRVHFPA
1160 1170 1180 1190
STDYSTQYGL KSTTNYVDFY STKRPSYRAE QYPGSPDSWV
Length:1,190
Mass (Da):131,551
Last modified:October 11, 2004 - v1
Checksum:iDB6548B5EBE72CFE
GO
Isoform 2 (identifier: Q68FH0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1042-1084: Missing.

Show »
Length:1,147
Mass (Da):126,783
Checksum:iDEC21F1C1F960B7A
GO
Isoform 3 (identifier: Q68FH0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     637-637: V → G
     638-1190: Missing.

Show »
Length:296
Mass (Da):33,263
Checksum:iD0630AA63A0911E8
GO

Sequence cautioni

The sequence BAC37187 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti328 – 3281S → F in AAH78638 (PubMed:15489334).Curated
Sequence conflicti877 – 8771V → A in BAC36708 (PubMed:16141072).Curated
Sequence conflicti1118 – 11181D → G in BAC36708 (PubMed:16141072).Curated
Sequence conflicti1161 – 11611K → R in BAB32313 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 341341Missing in isoform 3. 1 PublicationVSP_012374Add
BLAST
Alternative sequencei637 – 6371V → G in isoform 3. 1 PublicationVSP_012375
Alternative sequencei638 – 1190553Missing in isoform 3. 1 PublicationVSP_012376Add
BLAST
Alternative sequencei1042 – 108443Missing in isoform 2. 1 PublicationVSP_012377Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845536 Genomic DNA. Translation: CAM20481.1.
BC078638 mRNA. Translation: AAH78638.1.
BC079848 mRNA. Translation: AAH79848.1.
BC082578 mRNA. Translation: AAH82578.1.
AK021168 mRNA. Translation: BAB32313.1.
AK077250 mRNA. Translation: BAC36708.1.
AK078240 mRNA. Translation: BAC37187.1. Different initiation.
CCDSiCCDS16052.1. [Q68FH0-1]
CCDS50588.1. [Q68FH0-2]
RefSeqiNP_080637.1. NM_026361.2. [Q68FH0-1]
NP_780673.2. NM_175464.2. [Q68FH0-2]
XP_006499234.1. XM_006499171.2. [Q68FH0-1]
UniGeneiMm.260938.

Genome annotation databases

EnsembliENSMUST00000102754; ENSMUSP00000099815; ENSMUSG00000026991. [Q68FH0-1]
ENSMUST00000168631; ENSMUSP00000129836; ENSMUSG00000026991. [Q68FH0-2]
GeneIDi227937.
KEGGimmu:227937.
UCSCiuc008jtb.1. mouse. [Q68FH0-1]
uc008jtd.1. mouse. [Q68FH0-2]
uc008jtg.1. mouse. [Q68FH0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL845536 Genomic DNA. Translation: CAM20481.1.
BC078638 mRNA. Translation: AAH78638.1.
BC079848 mRNA. Translation: AAH79848.1.
BC082578 mRNA. Translation: AAH82578.1.
AK021168 mRNA. Translation: BAB32313.1.
AK077250 mRNA. Translation: BAC36708.1.
AK078240 mRNA. Translation: BAC37187.1. Different initiation.
CCDSiCCDS16052.1. [Q68FH0-1]
CCDS50588.1. [Q68FH0-2]
RefSeqiNP_080637.1. NM_026361.2. [Q68FH0-1]
NP_780673.2. NM_175464.2. [Q68FH0-2]
XP_006499234.1. XM_006499171.2. [Q68FH0-1]
UniGeneiMm.260938.

3D structure databases

ProteinModelPortaliQ68FH0.
SMRiQ68FH0. Positions 524-1010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230696. 3 interactions.
STRINGi10090.ENSMUSP00000099815.

PTM databases

iPTMnetiQ68FH0.
PhosphoSiteiQ68FH0.
SwissPalmiQ68FH0.

Proteomic databases

MaxQBiQ68FH0.
PaxDbiQ68FH0.
PeptideAtlasiQ68FH0.
PRIDEiQ68FH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102754; ENSMUSP00000099815; ENSMUSG00000026991. [Q68FH0-1]
ENSMUST00000168631; ENSMUSP00000129836; ENSMUSG00000026991. [Q68FH0-2]
GeneIDi227937.
KEGGimmu:227937.
UCSCiuc008jtb.1. mouse. [Q68FH0-1]
uc008jtd.1. mouse. [Q68FH0-2]
uc008jtg.1. mouse. [Q68FH0-3]

Organism-specific databases

CTDi8502.
MGIiMGI:109281. Pkp4.

Phylogenomic databases

eggNOGiKOG1048. Eukaryota.
ENOG410Y21Q. LUCA.
GeneTreeiENSGT00760000119167.
HOVERGENiHBG004284.
InParanoidiQ68FH0.
OMAiFHNSQNV.
OrthoDBiEOG091G00SB.
PhylomeDBiQ68FH0.
TreeFamiTF321877.

Miscellaneous databases

ChiTaRSiPkp4. mouse.
PROiQ68FH0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026991.
CleanExiMM_PKP4.
ExpressionAtlasiQ68FH0. baseline and differential.
GenevisibleiQ68FH0. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR028443. Plakophilin-4.
IPR028435. Plakophilin/d_Catenin.
[Graphical view]
PANTHERiPTHR10372. PTHR10372. 2 hits.
PTHR10372:SF8. PTHR10372:SF8. 2 hits.
PfamiPF00514. Arm. 3 hits.
[Graphical view]
SMARTiSM00185. ARM. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKP4_MOUSE
AccessioniPrimary (citable) accession number: Q68FH0
Secondary accession number(s): A2AS46
, Q640N0, Q68G56, Q8BK47, Q8BVH1, Q9CRE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.