Q68FF9 (S5A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 1
EC=1.3.1.22

Alternative name(s):
SR type 1
Steroid 5-alpha-reductase 1
Short name=S5AR 1

Gene names

Name:

Srd5a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology By similarity.
Catalytic activity	A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.
Subcellular location	Microsome membrane; Multi-pass membrane protein By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	androgen biosynthetic process Inferred from mutant phenotype PubMed 15249131. Source: MGI cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW sex differentiation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB cholestenone 5-alpha-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	3-oxo-5-alpha-steroid 4-dehydrogenase 1		PRO_0000317711
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	82 – 102	21	Helical; Potential
	Transmembrane	107 – 127	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	205 – 225	21	Helical; Potential
Experimental info
	Sequence conflict	7	1	C → R in AAH79863. Ref.1
	Sequence conflict	142	1	V → I in AAH94503. Ref.1
	Sequence conflict	176	1	E → D in AAH94503. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q68FF9 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 80A5624B25D46D1A	FASTA	255	29,344
	10 20 30 40 50 60 MELDELCLLD ALVYLEGFLA FVAFVGLQMV GSSYGRYSSQ WSGRRVPARP AWFLQELPSM 70 80 90 100 110 120 AWPLYECIRP AAARLGNLPN RVLLAMFLIH YVQRTLVFPV LIRGGKPTLL FTFVLAFLFC 130 140 150 160 170 180 TLNGYLQSRY LSQFAVYAED WVTHPCFLTG FALWLVGMVI NIHSDHILRN LRKPGETGYK 190 200 210 220 230 240 IPRGGLFEYV SSANYFGELV EWCGFALASW SLQGVVFALF TLCALFTRAR QHHQWYLEKF 250 EDYPKTRKIL IPFLL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC079863 mRNA. Translation: AAH79863.1. BC094503 mRNA. Translation: AAH94503.1.
IPI	IPI00470988.
RefSeq	NP_780492.2. NM_175283.3.
UniGene	Mm.451912. Mm.483181.
3D structure databases
ProteinModelPortal	Q68FF9.
ModBase	Search...
PTM databases
PhosphoSite	Q68FF9.
Proteomic databases
PaxDb	Q68FF9.
PRIDE	Q68FF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000091514; ENSMUSP00000089097; ENSMUSG00000021594.
GeneID	78925.
KEGG	mmu:78925.
UCSC	uc007rck.1. mouse.
Organism-specific databases
CTD	6715.
MGI	MGI:98400. Srd5a1.
Phylogenomic databases
eggNOG	NOG82739.
GeneTree	ENSGT00510000046634.
HOGENOM	HOG000050133.
HOVERGEN	HBG003402.
InParanoid	Q68FF9.
KO	K12343.
OrthoDB	EOG4VX25X.
Gene expression databases
Bgee	Q68FF9.
CleanEx	MM_SRD5A1.
Genevestigator	Q68FF9.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	349772.
SOURCE	Search...

Entry information

Entry name

S5A1_MOUSE

Accession

Primary (citable) accession number: Q68FF9
Secondary accession number(s): Q505K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	April 3, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program