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Q68FF6

- GIT1_MOUSE

UniProt

Q68FF6 - GIT1_MOUSE

Protein

ARF GTPase-activating protein GIT1

Gene

Git1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri11 – 3424C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ARF GTPase activator activity Source: InterPro
    2. protein binding Source: IntAct
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. regulation of ARF GTPase activity Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ARF GTPase-activating protein GIT1
    Short name:
    ARF GAP GIT1
    Alternative name(s):
    G protein-coupled receptor kinase-interactor 1
    GRK-interacting protein 1
    Gene namesi
    Name:Git1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1927140. Git1.

    Subcellular locationi

    Cytoplasm By similarity
    Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. focal adhesion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 770770ARF GTPase-activating protein GIT1PRO_0000074201Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei224 – 2241Phosphotyrosine1 Publication
    Modified residuei371 – 3711PhosphoserineBy similarity
    Modified residuei373 – 3731PhosphothreonineBy similarity
    Modified residuei384 – 3841PhosphoserineBy similarity
    Modified residuei392 – 3921PhosphotyrosineBy similarity
    Modified residuei394 – 3941PhosphoserineBy similarity
    Modified residuei397 – 3971PhosphoserineBy similarity
    Modified residuei401 – 4011PhosphothreonineBy similarity
    Modified residuei419 – 4191PhosphoserineBy similarity
    Modified residuei554 – 5541Phosphotyrosine1 Publication
    Modified residuei563 – 5631Phosphotyrosine1 Publication
    Modified residuei601 – 6011Phosphoserine1 Publication
    Modified residuei605 – 6051PhosphoserineBy similarity
    Modified residuei610 – 6101PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ68FF6.
    PaxDbiQ68FF6.
    PRIDEiQ68FF6.

    PTM databases

    PhosphoSiteiQ68FF6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ68FF6.
    BgeeiQ68FF6.
    CleanExiMM_GIT1.
    GenevestigatoriQ68FF6.

    Interactioni

    Subunit structurei

    Interacts with G protein-coupled receptor kinases: ADRBK1/GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with SCRIB By similarity. Interacts with TGFB1I1.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Arhgef7Q9ES282EBI-645933,EBI-642580

    Protein-protein interaction databases

    BioGridi229822. 7 interactions.
    IntActiQ68FF6. 19 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68FF6.
    SMRiQ68FF6. Positions 3-253, 427-482, 640-770.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 124124Arf-GAPPROSITE-ProRule annotationAdd
    BLAST
    Repeati132 – 16130ANK 1Add
    BLAST
    Repeati166 – 19530ANK 2Add
    BLAST
    Repeati199 – 22830ANK 3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni253 – 424172PTK2/FAK1-bindingBy similarityAdd
    BLAST
    Regioni254 – 376123ARHGEF6-bindingBy similarityAdd
    BLAST
    Regioni646 – 770125Interaction with PXN and TGFB1I1By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 3 ANK repeats.PROSITE-ProRule annotation
    Contains 1 Arf-GAP domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri11 – 3424C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5347.
    GeneTreeiENSGT00730000110864.
    HOGENOMiHOG000232135.
    HOVERGENiHBG012506.
    InParanoidiQ68FF6.
    KOiK05737.
    OMAiSARSQSD.
    OrthoDBiEOG7KQ212.
    PhylomeDBiQ68FF6.
    TreeFamiTF317762.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR022018. GIT1_C.
    IPR013724. GIT_SHD.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF12205. GIT1_C. 1 hit.
    PF08518. GIT_SHD. 2 hits.
    [Graphical view]
    PRINTSiPR00405. REVINTRACTNG.
    SMARTiSM00248. ANK. 3 hits.
    SM00105. ArfGap. 1 hit.
    SM00555. GIT. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 2 hits.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50115. ARFGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q68FF6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH    50
    LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH 100
    PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL 150
    RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG 200
    RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ 250
    MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE 300
    NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA 350
    TLIIDILSEA KRRQQGKSLS SPTDNLELSA RSQSELDDQH DYDSVASDED 400
    TDQEPLPSAG ATRNNRARSM DSSDLSDGAV TLQEYLELKK ALATSEAKVQ 450
    QLMKVNSSLS DELRRLQREI HKLQAENLQL RQPPGPVPPP SLPSERAEHT 500
    LMGPGGSTHR RDRQAFSMYE PGSALKPFGG TPGDELATRL QPFHSTELED 550
    DAIYSVHVPA GLYRIRKGVS ASSVPFTPSS PLLSCSQEGS RHASKLSRHG 600
    SGADSDYENT QSGDPLLGLE GKRFLELSKE DELHPELESL DGDLDPGLPS 650
    TEDVILKTEQ VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK 700
    RPALEPVRSS LRLLNASAYR LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA 750
    YDIAKAAKQL VTITTREKKQ 770
    Length:770
    Mass (Da):85,300
    Last modified:October 11, 2004 - v1
    Checksum:iC516E7A49578D0B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL607072 Genomic DNA. Translation: CAI52039.1.
    BC079870 mRNA. Translation: AAH79870.1.
    CCDSiCCDS25080.1.
    RefSeqiNP_001004144.1. NM_001004144.1.
    UniGeneiMm.290182.

    Genome annotation databases

    EnsembliENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877.
    GeneIDi216963.
    KEGGimmu:216963.
    UCSCiuc007kgy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL607072 Genomic DNA. Translation: CAI52039.1 .
    BC079870 mRNA. Translation: AAH79870.1 .
    CCDSi CCDS25080.1.
    RefSeqi NP_001004144.1. NM_001004144.1.
    UniGenei Mm.290182.

    3D structure databases

    ProteinModelPortali Q68FF6.
    SMRi Q68FF6. Positions 3-253, 427-482, 640-770.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229822. 7 interactions.
    IntActi Q68FF6. 19 interactions.

    PTM databases

    PhosphoSitei Q68FF6.

    Proteomic databases

    MaxQBi Q68FF6.
    PaxDbi Q68FF6.
    PRIDEi Q68FF6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037285 ; ENSMUSP00000037210 ; ENSMUSG00000011877 .
    GeneIDi 216963.
    KEGGi mmu:216963.
    UCSCi uc007kgy.1. mouse.

    Organism-specific databases

    CTDi 28964.
    MGIi MGI:1927140. Git1.

    Phylogenomic databases

    eggNOGi COG5347.
    GeneTreei ENSGT00730000110864.
    HOGENOMi HOG000232135.
    HOVERGENi HBG012506.
    InParanoidi Q68FF6.
    KOi K05737.
    OMAi SARSQSD.
    OrthoDBi EOG7KQ212.
    PhylomeDBi Q68FF6.
    TreeFami TF317762.

    Miscellaneous databases

    ChiTaRSi GIT1. mouse.
    NextBioi 375468.
    PROi Q68FF6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q68FF6.
    Bgeei Q68FF6.
    CleanExi MM_GIT1.
    Genevestigatori Q68FF6.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR022018. GIT1_C.
    IPR013724. GIT_SHD.
    [Graphical view ]
    Pfami PF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF12205. GIT1_C. 1 hit.
    PF08518. GIT_SHD. 2 hits.
    [Graphical view ]
    PRINTSi PR00405. REVINTRACTNG.
    SMARTi SM00248. ANK. 3 hits.
    SM00105. ArfGap. 1 hit.
    SM00555. GIT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 2 hits.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50115. ARFGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Hic-5 interacts with GIT1 with a different binding mode from paxillin."
      Nishiya N., Shirai T., Suzuki W., Nose K.
      J. Biochem. 132:279-289(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TGFB1I1.
    4. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-563, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-601, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-224, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-554, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiGIT1_MOUSE
    AccessioniPrimary (citable) accession number: Q68FF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3