Q68FD5 (CLH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Clathrin heavy chain 1

Gene names

Name:

Cltc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network By similarity.
Subunit structure	Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1 By similarity. Interacts with DENND1A, DENND1B and DENND1C. Interacts with ERBB2 By similarity. Interacts with OCRL. Ref.5
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side By similarity. Melanosome By similarity. Note: Cytoplasmic face of coated pits and vesicles By similarity.
Domain	The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.
Sequence similarities	Belongs to the clathrin heavy chain family.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Domain	Repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro receptor internalization Inferred from electronic annotation. Source: Compara spindle assembly involved in mitosis Inferred from sequence orthology PubMed 18548008. Source: MGI transferrin transport Inferred from electronic annotation. Source: Compara
Cellular_component	Myb complex Inferred from sequence orthology PubMed 18548008. Source: MGI clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell membrane coat Inferred from direct assay PubMed 11879655. Source: MGI mitochondrion Inferred from direct assay PubMed 14651853. Source: MGI spindle Inferred from electronic annotation. Source: Compara
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 1675	1674	Clathrin heavy chain 1		PRO_0000205779
Regions
	Region	2 – 479	478	Globular terminal domain
	Region	24 – 67	44	WD40-like repeat 1
	Region	68 – 107	40	WD40-like repeat 2
	Region	108 – 149	42	WD40-like repeat 3
	Region	150 – 195	46	WD40-like repeat 4
	Region	196 – 257	62	WD40-like repeat 5
	Region	258 – 301	44	WD40-like repeat 6
	Region	302 – 330	29	WD40-like repeat 7
	Region	449 – 465	17	Binding site for the uncoating ATPase, involved in lattice disassembly Potential
	Region	480 – 523	44	Flexible linker
	Region	524 – 1675	1152	Heavy chain arm
	Region	524 – 634	111	Distal segment
	Region	639 – 1675	1037	Proximal segment
	Region	1213 – 1522	310	Involved in binding clathrin light chain By similarity
	Region	1550 – 1675	126	Trimerization By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	184	1	Phosphotyrosine Ref.4
	Modified residue	394	1	Phosphothreonine By similarity
	Modified residue	634	1	Phosphotyrosine Ref.4
	Modified residue	856	1	N6-acetyllysine By similarity
	Modified residue	899	1	Phosphotyrosine Ref.4
	Modified residue	1206	1	Phosphotyrosine Ref.4
	Modified residue	1441	1	N6-acetyllysine By similarity
	Modified residue	1477	1	Phosphotyrosine Ref.4
	Modified residue	1487	1	Phosphotyrosine Ref.4
	Modified residue	1494	1	Phosphoserine By similarity
	Modified residue	1501	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q68FD5 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 6C41EBD89EB7D56B	FASTA	1,675	191,557
	10 20 30 40 50 60 MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV VIIDMNDPSN 70 80 90 100 110 120 PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK AHTMTDDVTF WKWISLNTVA 130 140 150 160 170 180 LVTDNAVYHW SMEGESQPVK MFDRHSSLAG CQIINYRTDA KQKWLLLTGI SAQQNRVVGA 190 200 210 220 230 240 MQLYSVDRKV SQPIEGHAAS FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN 250 260 270 280 290 300 QPFPKKAVDV FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG 310 320 330 340 350 360 ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR MAVRNNLAGA 370 380 390 400 410 420 EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR FQSVPAQPGQ TSPLLQYFGI 430 440 450 460 470 480 LLDQGQLNKY ESLELCRPVL QQGRKQLLEK WLKEDKLECS EELGDLVKSV DPTLALSVYL 490 500 510 520 530 540 RANVPNKVIQ CFAETGQVQK IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE 550 560 570 580 590 600 EPLADITQIV DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL 610 620 630 640 650 660 GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW LVNYFGSLSV 670 680 690 700 710 720 EDSLECLRAM LSANIRQNLQ ICVQVASKYH EQLSTQSLIE LFESFKSFEG LFYFLGSIVN 730 740 750 760 770 780 FSQDPDVHFK YIQAACKTGQ IKEVERICRE SNCYDPERVK NFLKEAKLTD QLPLIIVCDR 790 800 810 820 830 840 FDFVHDLVLY LYRNNLQKYI EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF 850 860 870 880 890 900 STDELVAEVE KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY 910 920 930 940 950 960 DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR KDPELWGSVL 970 980 990 1000 1010 1020 LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL PNELIELLEK IVLDNSVFSE 1030 1040 1050 1060 1070 1080 HRNLQNLLIL TAIKADRTRV MEYINRLDNY DAPDIANIAI SNELFEEAFA IFRKFDVNTS 1090 1100 1110 1120 1130 1140 AVQVLIEHIG NLDRAYEFAE RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV 1150 1160 1170 1180 1190 1200 VQAANASGNW EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ 1210 1220 1230 1240 1250 1260 VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST RTWKEVCFAC 1270 1280 1290 1300 1310 1320 VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI TMLEAALGLE RAHMGMFTEL 1330 1340 1350 1360 1370 1380 AILYSKFKPQ KMREHLELFW SRVNIPKVLR AAEQAHLWAE LVFLYDKYEE YDNAIITMMN 1390 1400 1410 1420 1430 1440 HPTDAWKEGQ FKDIITKVAN VELYYKAIQF YLEFKPLLLN DLLMVLSPRL DHTRAVNYFS 1450 1460 1470 1480 1490 1500 KVKQLPLVKP YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE 1510 1520 1530 1540 1550 1560 KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA EELLQWFLQE 1570 1580 1590 1600 1610 1620 EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI QVMKEYLTKV DKLDASESLR 1630 1640 1650 1660 1670 KEEEQATETQ PIVYGQPQLM LTAGPSVAVP PQAPFGYGYT APPYGQPQPG FGYSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 44-63; 87-96; 101-112; 145-157; 164-188; 190-205; 228-245; 270-278; 298-320; 355-366; 377-382; 430-444; 446-453; 457-481; 626-637; 768-780; 799-806; 838-852 AND 857-881. Strain: C57BL/6. Tissue: Brain.
[4]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-184; TYR-634; TYR-899; TYR-1206; TYR-1477 AND TYR-1487, MASS SPECTROMETRY. Tissue: Brain.
[5]	"Two closely related endocytic proteins that share a common OCRL-binding motif with APPL1." Swan L.E., Tomasini L., Pirruccello M., Lunardi J., De Camilli P. Proc. Natl. Acad. Sci. U.S.A. 107:3511-3516(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OCRL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL592222 Genomic DNA. Translation: CAI25362.1. BC079897 mRNA. Translation: AAH79897.1.
IPI	IPI00169916.
RefSeq	NP_001003908.1. NM_001003908.1.
UniGene	Mm.479526.
3D structure databases
ProteinModelPortal	Q68FD5.
SMR	Q68FD5. Positions 1-493, 1077-1630.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q68FD5. 16 interactions.
PTM databases
PhosphoSite	Q68FD5.
Proteomic databases
PaxDb	Q68FD5.
PRIDE	Q68FD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103186; ENSMUSP00000099475; ENSMUSG00000047126.
GeneID	67300.
KEGG	mmu:67300.
UCSC	uc007kta.1. mouse.
Organism-specific databases
CTD	1213.
MGI	MGI:2388633. Cltc.
Phylogenomic databases
eggNOG	NOG314149.
GeneTree	ENSGT00400000022107.
HOVERGEN	HBG005344.
KO	K04646.
OrthoDB	EOG4TXBR0.
Gene expression databases
ArrayExpress	Q68FD5.
Bgee	Q68FD5.
CleanEx	MM_CLTC.
Genevestigator	Q68FD5.
GermOnline	ENSMUSG00000047126. Mus musculus.
Family and domain databases
Gene3D	1.25.40.10. 4 hits. 2.130.10.110. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR000547. Clathrin_H-chain/VPS_repeat. IPR016025. Clathrin_H-chain_link/propller. IPR015348. Clathrin_H-chain_linker_core. IPR001473. Clathrin_H-chain_propeller_N. IPR022365. Clathrin_H-chain_propeller_rpt. IPR016341. Clathrin_heavy_chain. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF00637. Clathrin. 7 hits. PF09268. Clathrin-link. 1 hit. PF01394. Clathrin_propel. 3 hits. [Graphical view]
PIRSF	PIRSF002290. Clathrin_H_chain. 1 hit.
SMART	SM00299. CLH. 7 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 5 hits. SSF50989. Clathrin_H-chain_propeller_N. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	CLTC. mouse.
NextBio	324168.
SOURCE	Search...

Entry information

Entry name

CLH1_MOUSE

Accession

Primary (citable) accession number: Q68FD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2005
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program