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Q68FD5

- CLH1_MOUSE

UniProt

Q68FD5 - CLH1_MOUSE

Protein

Clathrin heavy chain 1

Gene

Cltc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network By similarity.By similarity

    GO - Molecular functioni

    1. double-stranded RNA binding Source: MGI
    2. protein kinase binding Source: ParkinsonsUK-UCL
    3. structural molecule activity Source: InterPro

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. negative regulation of hyaluronan biosynthetic process Source: UniProtKB
    3. receptor internalization Source: Ensembl
    4. spindle assembly involved in mitosis Source: MGI
    5. transferrin transport Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_222404. WNT5A-dependent internalization of FZD4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Clathrin heavy chain 1
    Gene namesi
    Name:Cltc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2388633. Cltc.

    Subcellular locationi

    Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Membranecoated pit By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Melanosome By similarity
    Note: Cytoplasmic face of coated pits and vesicles.By similarity

    GO - Cellular componenti

    1. clathrin coat of coated pit Source: InterPro
    2. clathrin coat of trans-Golgi network vesicle Source: InterPro
    3. clathrin complex Source: Ensembl
    4. extracellular vesicular exosome Source: Ensembl
    5. melanosome Source: UniProtKB-SubCell
    6. membrane coat Source: MGI
    7. mitochondrion Source: MGI
    8. Myb complex Source: MGI
    9. protein complex Source: MGI
    10. spindle Source: Ensembl

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 16751674Clathrin heavy chain 1PRO_0000205779Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei184 – 1841Phosphotyrosine1 Publication
    Modified residuei394 – 3941PhosphothreonineBy similarity
    Modified residuei634 – 6341Phosphotyrosine1 Publication
    Modified residuei737 – 7371N6-succinyllysine1 Publication
    Modified residuei856 – 8561N6-acetyllysineBy similarity
    Modified residuei899 – 8991Phosphotyrosine1 Publication
    Modified residuei1206 – 12061Phosphotyrosine1 Publication
    Modified residuei1441 – 14411N6-acetyllysine; alternateBy similarity
    Modified residuei1441 – 14411N6-succinyllysine; alternate1 Publication
    Modified residuei1477 – 14771Phosphotyrosine1 Publication
    Modified residuei1487 – 14871Phosphotyrosine1 Publication
    Modified residuei1494 – 14941PhosphoserineBy similarity
    Modified residuei1501 – 15011N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ68FD5.
    PaxDbiQ68FD5.
    PRIDEiQ68FD5.

    PTM databases

    PhosphoSiteiQ68FD5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ68FD5.
    BgeeiQ68FD5.
    CleanExiMM_CLTC.
    GenevestigatoriQ68FD5.

    Interactioni

    Subunit structurei

    Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1 By similarity. Interacts with DENND1A, DENND1B and DENND1C. Interacts with ERBB2. Interacts with FKBP6 By similarity. Interacts with OCRL.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi212086. 12 interactions.
    DIPiDIP-31947N.
    IntActiQ68FD5. 25 interactions.
    MINTiMINT-4153227.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68FD5.
    SMRiQ68FD5. Positions 1-493, 1077-1630.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati537 – 683147CHCR 1Add
    BLAST
    Repeati686 – 828143CHCR 2Add
    BLAST
    Repeati833 – 972140CHCR 3Add
    BLAST
    Repeati979 – 1124146CHCR 4Add
    BLAST
    Repeati1128 – 1269142CHCR 5Add
    BLAST
    Repeati1274 – 1420147CHCR 6Add
    BLAST
    Repeati1423 – 1566144CHCR 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 479478Globular terminal domainAdd
    BLAST
    Regioni24 – 6744WD40-like repeat 1Add
    BLAST
    Regioni68 – 10740WD40-like repeat 2Add
    BLAST
    Regioni108 – 14942WD40-like repeat 3Add
    BLAST
    Regioni150 – 19546WD40-like repeat 4Add
    BLAST
    Regioni196 – 25762WD40-like repeat 5Add
    BLAST
    Regioni258 – 30144WD40-like repeat 6Add
    BLAST
    Regioni302 – 33029WD40-like repeat 7Add
    BLAST
    Regioni449 – 46517Binding site for the uncoating ATPase, involved in lattice disassemblySequence AnalysisAdd
    BLAST
    Regioni480 – 52344Flexible linkerAdd
    BLAST
    Regioni524 – 16751152Heavy chain armAdd
    BLAST
    Regioni524 – 634111Distal segmentAdd
    BLAST
    Regioni639 – 16751037Proximal segmentAdd
    BLAST
    Regioni1213 – 1522310Involved in binding clathrin light chainBy similarityAdd
    BLAST
    Regioni1550 – 1675126TrimerizationBy similarityAdd
    BLAST

    Domaini

    The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node By similarity.By similarity

    Sequence similaritiesi

    Belongs to the clathrin heavy chain family.Curated
    Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG314149.
    GeneTreeiENSGT00400000022107.
    HOVERGENiHBG005344.
    KOiK04646.
    OrthoDBiEOG7Z0JVM.
    TreeFamiTF300059.

    Family and domain databases

    Gene3Di1.25.40.10. 4 hits.
    2.130.10.110. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016025. Clathrin_H-chain_link/propller.
    IPR015348. Clathrin_H-chain_linker_core.
    IPR001473. Clathrin_H-chain_propeller_N.
    IPR022365. Clathrin_H-chain_propeller_rpt.
    IPR016341. Clathrin_heavy_chain.
    IPR011990. TPR-like_helical.
    [Graphical view]
    PfamiPF00637. Clathrin. 7 hits.
    PF09268. Clathrin-link. 1 hit.
    PF01394. Clathrin_propel. 3 hits.
    [Graphical view]
    PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
    SMARTiSM00299. CLH. 7 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 6 hits.
    SSF50989. SSF50989. 1 hit.
    PROSITEiPS50236. CHCR. 7 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q68FD5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV     50
    VIIDMNDPSN PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK 100
    AHTMTDDVTF WKWISLNTVA LVTDNAVYHW SMEGESQPVK MFDRHSSLAG 150
    CQIINYRTDA KQKWLLLTGI SAQQNRVVGA MQLYSVDRKV SQPIEGHAAS 200
    FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN QPFPKKAVDV 250
    FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG 300
    ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR 350
    MAVRNNLAGA EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR 400
    FQSVPAQPGQ TSPLLQYFGI LLDQGQLNKY ESLELCRPVL QQGRKQLLEK 450
    WLKEDKLECS EELGDLVKSV DPTLALSVYL RANVPNKVIQ CFAETGQVQK 500
    IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE EPLADITQIV 550
    DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL 600
    GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW 650
    LVNYFGSLSV EDSLECLRAM LSANIRQNLQ ICVQVASKYH EQLSTQSLIE 700
    LFESFKSFEG LFYFLGSIVN FSQDPDVHFK YIQAACKTGQ IKEVERICRE 750
    SNCYDPERVK NFLKEAKLTD QLPLIIVCDR FDFVHDLVLY LYRNNLQKYI 800
    EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF STDELVAEVE 850
    KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY 900
    DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR 950
    KDPELWGSVL LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL 1000
    PNELIELLEK IVLDNSVFSE HRNLQNLLIL TAIKADRTRV MEYINRLDNY 1050
    DAPDIANIAI SNELFEEAFA IFRKFDVNTS AVQVLIEHIG NLDRAYEFAE 1100
    RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV VQAANASGNW 1150
    EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ 1200
    VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST 1250
    RTWKEVCFAC VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI 1300
    TMLEAALGLE RAHMGMFTEL AILYSKFKPQ KMREHLELFW SRVNIPKVLR 1350
    AAEQAHLWAE LVFLYDKYEE YDNAIITMMN HPTDAWKEGQ FKDIITKVAN 1400
    VELYYKAIQF YLEFKPLLLN DLLMVLSPRL DHTRAVNYFS KVKQLPLVKP 1450
    YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE 1500
    KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA 1550
    EELLQWFLQE EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI 1600
    QVMKEYLTKV DKLDASESLR KEEEQATETQ PIVYGQPQLM LTAGPSVAVP 1650
    PQAPFGYGYT APPYGQPQPG FGYSM 1675
    Length:1,675
    Mass (Da):191,557
    Last modified:January 23, 2007 - v3
    Checksum:i6C41EBD89EB7D56B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL592222 Genomic DNA. Translation: CAI25362.1.
    BC079897 mRNA. Translation: AAH79897.1.
    CCDSiCCDS25204.1.
    RefSeqiNP_001003908.1. NM_001003908.1.
    UniGeneiMm.254588.

    Genome annotation databases

    EnsembliENSMUST00000103186; ENSMUSP00000099475; ENSMUSG00000047126.
    GeneIDi67300.
    KEGGimmu:67300.
    UCSCiuc007kta.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL592222 Genomic DNA. Translation: CAI25362.1 .
    BC079897 mRNA. Translation: AAH79897.1 .
    CCDSi CCDS25204.1.
    RefSeqi NP_001003908.1. NM_001003908.1.
    UniGenei Mm.254588.

    3D structure databases

    ProteinModelPortali Q68FD5.
    SMRi Q68FD5. Positions 1-493, 1077-1630.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212086. 12 interactions.
    DIPi DIP-31947N.
    IntActi Q68FD5. 25 interactions.
    MINTi MINT-4153227.

    PTM databases

    PhosphoSitei Q68FD5.

    Proteomic databases

    MaxQBi Q68FD5.
    PaxDbi Q68FD5.
    PRIDEi Q68FD5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103186 ; ENSMUSP00000099475 ; ENSMUSG00000047126 .
    GeneIDi 67300.
    KEGGi mmu:67300.
    UCSCi uc007kta.1. mouse.

    Organism-specific databases

    CTDi 1213.
    MGIi MGI:2388633. Cltc.

    Phylogenomic databases

    eggNOGi NOG314149.
    GeneTreei ENSGT00400000022107.
    HOVERGENi HBG005344.
    KOi K04646.
    OrthoDBi EOG7Z0JVM.
    TreeFami TF300059.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_222404. WNT5A-dependent internalization of FZD4.

    Miscellaneous databases

    ChiTaRSi CLTC. mouse.
    NextBioi 324168.
    PROi Q68FD5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q68FD5.
    Bgeei Q68FD5.
    CleanExi MM_CLTC.
    Genevestigatori Q68FD5.

    Family and domain databases

    Gene3Di 1.25.40.10. 4 hits.
    2.130.10.110. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016025. Clathrin_H-chain_link/propller.
    IPR015348. Clathrin_H-chain_linker_core.
    IPR001473. Clathrin_H-chain_propeller_N.
    IPR022365. Clathrin_H-chain_propeller_rpt.
    IPR016341. Clathrin_heavy_chain.
    IPR011990. TPR-like_helical.
    [Graphical view ]
    Pfami PF00637. Clathrin. 7 hits.
    PF09268. Clathrin-link. 1 hit.
    PF01394. Clathrin_propel. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF002290. Clathrin_H_chain. 1 hit.
    SMARTi SM00299. CLH. 7 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 6 hits.
    SSF50989. SSF50989. 1 hit.
    PROSITEi PS50236. CHCR. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-184; TYR-634; TYR-899; TYR-1206; TYR-1477 AND TYR-1487, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    5. "Two closely related endocytic proteins that share a common OCRL-binding motif with APPL1."
      Swan L.E., Tomasini L., Pirruccello M., Lunardi J., De Camilli P.
      Proc. Natl. Acad. Sci. U.S.A. 107:3511-3516(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OCRL.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-737 AND LYS-1441, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiCLH1_MOUSE
    AccessioniPrimary (citable) accession number: Q68FD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3