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Protein

Clathrin heavy chain 1

Gene

Cltc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-171052. LDL-mediated lipid transport.
R-MMU-177504. Retrograde neurotrophin signalling.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-437239. Recycling pathway of L1.
R-MMU-5099900. WNT5A-dependent internalization of FZD4.
R-MMU-5140745. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.
R-MMU-8866427. VLDLR internalisation and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin heavy chain 1
Gene namesi
Name:Cltc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2388633. Cltc.

Subcellular locationi

GO - Cellular componenti

  • clathrin-coated vesicle Source: MGI
  • clathrin coat of coated pit Source: InterPro
  • clathrin coat of trans-Golgi network vesicle Source: InterPro
  • clathrin complex Source: MGI
  • cytosol Source: GOC
  • extracellular exosome Source: MGI
  • extracellular matrix Source: MGI
  • extracellular vesicle Source: MGI
  • focal adhesion Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: ParkinsonsUK-UCL
  • membrane coat Source: MGI
  • mitochondrion Source: MGI
  • mitotic spindle microtubule Source: MGI
  • Myb complex Source: MGI
  • myelin sheath Source: UniProtKB
  • protein complex Source: MGI
  • spindle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002057792 – 1675Clathrin heavy chain 1Add BLAST1674

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei67PhosphoserineCombined sources1
Modified residuei105PhosphothreonineCombined sources1
Modified residuei184PhosphotyrosineCombined sources1
Modified residuei394PhosphothreonineBy similarity1
Modified residuei634PhosphotyrosineCombined sources1
Modified residuei737N6-succinyllysineCombined sources1
Modified residuei856N6-acetyllysineBy similarity1
Modified residuei899PhosphotyrosineCombined sources1
Modified residuei1167PhosphoserineCombined sources1
Modified residuei1206PhosphotyrosineCombined sources1
Modified residuei1229PhosphoserineBy similarity1
Modified residuei1441N6-acetyllysine; alternateBy similarity1
Modified residuei1441N6-succinyllysine; alternateCombined sources1
Modified residuei1477PhosphotyrosineCombined sources1
Modified residuei1487PhosphotyrosineCombined sources1
Modified residuei1494PhosphoserineCombined sources1
Modified residuei1501N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ68FD5.
MaxQBiQ68FD5.
PaxDbiQ68FD5.
PRIDEiQ68FD5.

PTM databases

iPTMnetiQ68FD5.
PhosphoSitePlusiQ68FD5.
SwissPalmiQ68FD5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047126.
CleanExiMM_CLTC.
ExpressionAtlasiQ68FD5. baseline and differential.
GenevisibleiQ68FD5. MM.

Interactioni

Subunit structurei

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat (By similarity). In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (By similarity). Interacts with HIP1 (By similarity). Interacts with DENND1A, DENND1B and DENND1C (By similarity). Interacts with ERBB2 (By similarity). Interacts with FKBP6 (By similarity). Interacts with OCRL (PubMed:20133602). Interacts with CKAP5 and TACC3 forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; the complex implicates clathrin triskelions; TACC3 and CLTC are proposed to form a composite microtubule interaction surface (By similarity). Plays a role in early autopahgosome formation (By similarity). Interacts with ATG16L1 (via N-terminus) (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SF1Q156373EBI-769168,EBI-744603From a different organism.

GO - Molecular functioni

  • clathrin light chain binding Source: MGI
  • protein kinase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi212086. 15 interactors.
DIPiDIP-31947N.
IntActiQ68FD5. 29 interactors.
MINTiMINT-4153227.
STRINGi10090.ENSMUSP00000099475.

Structurei

3D structure databases

ProteinModelPortaliQ68FD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati537 – 683CHCR 1Add BLAST147
Repeati686 – 828CHCR 2Add BLAST143
Repeati833 – 972CHCR 3Add BLAST140
Repeati979 – 1124CHCR 4Add BLAST146
Repeati1128 – 1269CHCR 5Add BLAST142
Repeati1274 – 1420CHCR 6Add BLAST147
Repeati1423 – 1566CHCR 7Add BLAST144

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 479Globular terminal domainAdd BLAST478
Regioni24 – 67WD40-like repeat 1Add BLAST44
Regioni68 – 107WD40-like repeat 2Add BLAST40
Regioni108 – 149WD40-like repeat 3Add BLAST42
Regioni150 – 195WD40-like repeat 4Add BLAST46
Regioni196 – 257WD40-like repeat 5Add BLAST62
Regioni258 – 301WD40-like repeat 6Add BLAST44
Regioni302 – 330WD40-like repeat 7Add BLAST29
Regioni449 – 465Binding site for the uncoating ATPase, involved in lattice disassemblySequence analysisAdd BLAST17
Regioni480 – 523Flexible linkerAdd BLAST44
Regioni524 – 1675Heavy chain armAdd BLAST1152
Regioni524 – 634Distal segmentAdd BLAST111
Regioni639 – 1675Proximal segmentAdd BLAST1037
Regioni1213 – 1522Involved in binding clathrin light chainBy similarityAdd BLAST310
Regioni1550 – 1675TrimerizationBy similarityAdd BLAST126

Domaini

The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node (By similarity).By similarity

Sequence similaritiesi

Belongs to the clathrin heavy chain family.Curated
Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0985. Eukaryota.
ENOG410XPH1. LUCA.
GeneTreeiENSGT00400000022107.
HOVERGENiHBG005344.
InParanoidiQ68FD5.
KOiK04646.
TreeFamiTF300059.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 5 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV
60 70 80 90 100
VIIDMNDPSN PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK
110 120 130 140 150
AHTMTDDVTF WKWISLNTVA LVTDNAVYHW SMEGESQPVK MFDRHSSLAG
160 170 180 190 200
CQIINYRTDA KQKWLLLTGI SAQQNRVVGA MQLYSVDRKV SQPIEGHAAS
210 220 230 240 250
FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN QPFPKKAVDV
260 270 280 290 300
FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG
310 320 330 340 350
ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR
360 370 380 390 400
MAVRNNLAGA EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR
410 420 430 440 450
FQSVPAQPGQ TSPLLQYFGI LLDQGQLNKY ESLELCRPVL QQGRKQLLEK
460 470 480 490 500
WLKEDKLECS EELGDLVKSV DPTLALSVYL RANVPNKVIQ CFAETGQVQK
510 520 530 540 550
IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE EPLADITQIV
560 570 580 590 600
DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL
610 620 630 640 650
GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW
660 670 680 690 700
LVNYFGSLSV EDSLECLRAM LSANIRQNLQ ICVQVASKYH EQLSTQSLIE
710 720 730 740 750
LFESFKSFEG LFYFLGSIVN FSQDPDVHFK YIQAACKTGQ IKEVERICRE
760 770 780 790 800
SNCYDPERVK NFLKEAKLTD QLPLIIVCDR FDFVHDLVLY LYRNNLQKYI
810 820 830 840 850
EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF STDELVAEVE
860 870 880 890 900
KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY
910 920 930 940 950
DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR
960 970 980 990 1000
KDPELWGSVL LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL
1010 1020 1030 1040 1050
PNELIELLEK IVLDNSVFSE HRNLQNLLIL TAIKADRTRV MEYINRLDNY
1060 1070 1080 1090 1100
DAPDIANIAI SNELFEEAFA IFRKFDVNTS AVQVLIEHIG NLDRAYEFAE
1110 1120 1130 1140 1150
RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV VQAANASGNW
1160 1170 1180 1190 1200
EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ
1210 1220 1230 1240 1250
VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST
1260 1270 1280 1290 1300
RTWKEVCFAC VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI
1310 1320 1330 1340 1350
TMLEAALGLE RAHMGMFTEL AILYSKFKPQ KMREHLELFW SRVNIPKVLR
1360 1370 1380 1390 1400
AAEQAHLWAE LVFLYDKYEE YDNAIITMMN HPTDAWKEGQ FKDIITKVAN
1410 1420 1430 1440 1450
VELYYKAIQF YLEFKPLLLN DLLMVLSPRL DHTRAVNYFS KVKQLPLVKP
1460 1470 1480 1490 1500
YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE
1510 1520 1530 1540 1550
KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA
1560 1570 1580 1590 1600
EELLQWFLQE EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI
1610 1620 1630 1640 1650
QVMKEYLTKV DKLDASESLR KEEEQATETQ PIVYGQPQLM LTAGPSVAVP
1660 1670
PQAPFGYGYT APPYGQPQPG FGYSM
Length:1,675
Mass (Da):191,557
Last modified:January 23, 2007 - v3
Checksum:i6C41EBD89EB7D56B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL592222 Genomic DNA. Translation: CAI25362.1.
BC079897 mRNA. Translation: AAH79897.1.
CCDSiCCDS25204.1.
RefSeqiNP_001003908.1. NM_001003908.1.
UniGeneiMm.254588.

Genome annotation databases

EnsembliENSMUST00000103186; ENSMUSP00000099475; ENSMUSG00000047126.
GeneIDi67300.
KEGGimmu:67300.
UCSCiuc007kta.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL592222 Genomic DNA. Translation: CAI25362.1.
BC079897 mRNA. Translation: AAH79897.1.
CCDSiCCDS25204.1.
RefSeqiNP_001003908.1. NM_001003908.1.
UniGeneiMm.254588.

3D structure databases

ProteinModelPortaliQ68FD5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212086. 15 interactors.
DIPiDIP-31947N.
IntActiQ68FD5. 29 interactors.
MINTiMINT-4153227.
STRINGi10090.ENSMUSP00000099475.

PTM databases

iPTMnetiQ68FD5.
PhosphoSitePlusiQ68FD5.
SwissPalmiQ68FD5.

Proteomic databases

EPDiQ68FD5.
MaxQBiQ68FD5.
PaxDbiQ68FD5.
PRIDEiQ68FD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103186; ENSMUSP00000099475; ENSMUSG00000047126.
GeneIDi67300.
KEGGimmu:67300.
UCSCiuc007kta.1. mouse.

Organism-specific databases

CTDi1213.
MGIiMGI:2388633. Cltc.

Phylogenomic databases

eggNOGiKOG0985. Eukaryota.
ENOG410XPH1. LUCA.
GeneTreeiENSGT00400000022107.
HOVERGENiHBG005344.
InParanoidiQ68FD5.
KOiK04646.
TreeFamiTF300059.

Enzyme and pathway databases

ReactomeiR-MMU-171052. LDL-mediated lipid transport.
R-MMU-177504. Retrograde neurotrophin signalling.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-437239. Recycling pathway of L1.
R-MMU-5099900. WNT5A-dependent internalization of FZD4.
R-MMU-5140745. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.
R-MMU-8866427. VLDLR internalisation and degradation.

Miscellaneous databases

ChiTaRSiCltc. mouse.
PROiQ68FD5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047126.
CleanExiMM_CLTC.
ExpressionAtlasiQ68FD5. baseline and differential.
GenevisibleiQ68FD5. MM.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 5 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLH1_MOUSE
AccessioniPrimary (citable) accession number: Q68FD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.