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Protein

Clathrin heavy chain 1

Gene

Cltc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_299462. WNT5A-dependent internalization of FZD4.
REACT_303468. Lysosome Vesicle Biogenesis.
REACT_316383. MHC class II antigen presentation.
REACT_342454. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin heavy chain 1
Gene namesi
Name:Cltc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2388633. Cltc.

Subcellular locationi

GO - Cellular componenti

  • clathrin-coated vesicle Source: MGI
  • clathrin coat of coated pit Source: InterPro
  • clathrin coat of trans-Golgi network vesicle Source: InterPro
  • clathrin complex Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • extracellular vesicle Source: MGI
  • focal adhesion Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: ParkinsonsUK-UCL
  • membrane coat Source: MGI
  • mitochondrion Source: MGI
  • Myb complex Source: MGI
  • myelin sheath Source: UniProtKB
  • protein complex Source: MGI
  • spindle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 16751674Clathrin heavy chain 1PRO_0000205779Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei184 – 1841Phosphotyrosine1 Publication
Modified residuei394 – 3941PhosphothreonineBy similarity
Modified residuei634 – 6341Phosphotyrosine1 Publication
Modified residuei737 – 7371N6-succinyllysine1 Publication
Modified residuei856 – 8561N6-acetyllysineBy similarity
Modified residuei899 – 8991Phosphotyrosine1 Publication
Modified residuei1206 – 12061Phosphotyrosine1 Publication
Modified residuei1441 – 14411N6-acetyllysine; alternateBy similarity
Modified residuei1441 – 14411N6-succinyllysine; alternate1 Publication
Modified residuei1477 – 14771Phosphotyrosine1 Publication
Modified residuei1487 – 14871Phosphotyrosine1 Publication
Modified residuei1494 – 14941PhosphoserineBy similarity
Modified residuei1501 – 15011N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ68FD5.
PaxDbiQ68FD5.
PRIDEiQ68FD5.

PTM databases

PhosphoSiteiQ68FD5.

Expressioni

Gene expression databases

BgeeiQ68FD5.
CleanExiMM_CLTC.
ExpressionAtlasiQ68FD5. baseline and differential.
GenevisibleiQ68FD5. MM.

Interactioni

Subunit structurei

Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1 (By similarity). Interacts with DENND1A, DENND1B and DENND1C. Interacts with ERBB2. Interacts with FKBP6 (By similarity). Interacts with OCRL.By similarity1 Publication

Protein-protein interaction databases

BioGridi212086. 14 interactions.
DIPiDIP-31947N.
IntActiQ68FD5. 27 interactions.
MINTiMINT-4153227.
STRINGi10090.ENSMUSP00000099475.

Structurei

3D structure databases

ProteinModelPortaliQ68FD5.
SMRiQ68FD5. Positions 1-493, 1077-1630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati537 – 683147CHCR 1Add
BLAST
Repeati686 – 828143CHCR 2Add
BLAST
Repeati833 – 972140CHCR 3Add
BLAST
Repeati979 – 1124146CHCR 4Add
BLAST
Repeati1128 – 1269142CHCR 5Add
BLAST
Repeati1274 – 1420147CHCR 6Add
BLAST
Repeati1423 – 1566144CHCR 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 479478Globular terminal domainAdd
BLAST
Regioni24 – 6744WD40-like repeat 1Add
BLAST
Regioni68 – 10740WD40-like repeat 2Add
BLAST
Regioni108 – 14942WD40-like repeat 3Add
BLAST
Regioni150 – 19546WD40-like repeat 4Add
BLAST
Regioni196 – 25762WD40-like repeat 5Add
BLAST
Regioni258 – 30144WD40-like repeat 6Add
BLAST
Regioni302 – 33029WD40-like repeat 7Add
BLAST
Regioni449 – 46517Binding site for the uncoating ATPase, involved in lattice disassemblySequence AnalysisAdd
BLAST
Regioni480 – 52344Flexible linkerAdd
BLAST
Regioni524 – 16751152Heavy chain armAdd
BLAST
Regioni524 – 634111Distal segmentAdd
BLAST
Regioni639 – 16751037Proximal segmentAdd
BLAST
Regioni1213 – 1522310Involved in binding clathrin light chainBy similarityAdd
BLAST
Regioni1550 – 1675126TrimerizationBy similarityAdd
BLAST

Domaini

The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It consitutes a major protein-protein interaction node (By similarity).By similarity

Sequence similaritiesi

Belongs to the clathrin heavy chain family.Curated
Contains 7 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG314149.
GeneTreeiENSGT00400000022107.
HOVERGENiHBG005344.
InParanoidiQ68FD5.
KOiK04646.
OrthoDBiEOG7Z0JVM.
TreeFamiTF300059.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 3 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68FD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV
60 70 80 90 100
VIIDMNDPSN PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK
110 120 130 140 150
AHTMTDDVTF WKWISLNTVA LVTDNAVYHW SMEGESQPVK MFDRHSSLAG
160 170 180 190 200
CQIINYRTDA KQKWLLLTGI SAQQNRVVGA MQLYSVDRKV SQPIEGHAAS
210 220 230 240 250
FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN QPFPKKAVDV
260 270 280 290 300
FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG
310 320 330 340 350
ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR
360 370 380 390 400
MAVRNNLAGA EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR
410 420 430 440 450
FQSVPAQPGQ TSPLLQYFGI LLDQGQLNKY ESLELCRPVL QQGRKQLLEK
460 470 480 490 500
WLKEDKLECS EELGDLVKSV DPTLALSVYL RANVPNKVIQ CFAETGQVQK
510 520 530 540 550
IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE EPLADITQIV
560 570 580 590 600
DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL
610 620 630 640 650
GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW
660 670 680 690 700
LVNYFGSLSV EDSLECLRAM LSANIRQNLQ ICVQVASKYH EQLSTQSLIE
710 720 730 740 750
LFESFKSFEG LFYFLGSIVN FSQDPDVHFK YIQAACKTGQ IKEVERICRE
760 770 780 790 800
SNCYDPERVK NFLKEAKLTD QLPLIIVCDR FDFVHDLVLY LYRNNLQKYI
810 820 830 840 850
EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF STDELVAEVE
860 870 880 890 900
KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY
910 920 930 940 950
DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR
960 970 980 990 1000
KDPELWGSVL LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL
1010 1020 1030 1040 1050
PNELIELLEK IVLDNSVFSE HRNLQNLLIL TAIKADRTRV MEYINRLDNY
1060 1070 1080 1090 1100
DAPDIANIAI SNELFEEAFA IFRKFDVNTS AVQVLIEHIG NLDRAYEFAE
1110 1120 1130 1140 1150
RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV VQAANASGNW
1160 1170 1180 1190 1200
EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ
1210 1220 1230 1240 1250
VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST
1260 1270 1280 1290 1300
RTWKEVCFAC VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI
1310 1320 1330 1340 1350
TMLEAALGLE RAHMGMFTEL AILYSKFKPQ KMREHLELFW SRVNIPKVLR
1360 1370 1380 1390 1400
AAEQAHLWAE LVFLYDKYEE YDNAIITMMN HPTDAWKEGQ FKDIITKVAN
1410 1420 1430 1440 1450
VELYYKAIQF YLEFKPLLLN DLLMVLSPRL DHTRAVNYFS KVKQLPLVKP
1460 1470 1480 1490 1500
YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE
1510 1520 1530 1540 1550
KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA
1560 1570 1580 1590 1600
EELLQWFLQE EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI
1610 1620 1630 1640 1650
QVMKEYLTKV DKLDASESLR KEEEQATETQ PIVYGQPQLM LTAGPSVAVP
1660 1670
PQAPFGYGYT APPYGQPQPG FGYSM
Length:1,675
Mass (Da):191,557
Last modified:January 23, 2007 - v3
Checksum:i6C41EBD89EB7D56B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL592222 Genomic DNA. Translation: CAI25362.1.
BC079897 mRNA. Translation: AAH79897.1.
CCDSiCCDS25204.1.
RefSeqiNP_001003908.1. NM_001003908.1.
UniGeneiMm.254588.

Genome annotation databases

EnsembliENSMUST00000103186; ENSMUSP00000099475; ENSMUSG00000047126.
GeneIDi67300.
KEGGimmu:67300.
UCSCiuc007kta.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL592222 Genomic DNA. Translation: CAI25362.1.
BC079897 mRNA. Translation: AAH79897.1.
CCDSiCCDS25204.1.
RefSeqiNP_001003908.1. NM_001003908.1.
UniGeneiMm.254588.

3D structure databases

ProteinModelPortaliQ68FD5.
SMRiQ68FD5. Positions 1-493, 1077-1630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212086. 14 interactions.
DIPiDIP-31947N.
IntActiQ68FD5. 27 interactions.
MINTiMINT-4153227.
STRINGi10090.ENSMUSP00000099475.

PTM databases

PhosphoSiteiQ68FD5.

Proteomic databases

MaxQBiQ68FD5.
PaxDbiQ68FD5.
PRIDEiQ68FD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103186; ENSMUSP00000099475; ENSMUSG00000047126.
GeneIDi67300.
KEGGimmu:67300.
UCSCiuc007kta.1. mouse.

Organism-specific databases

CTDi1213.
MGIiMGI:2388633. Cltc.

Phylogenomic databases

eggNOGiNOG314149.
GeneTreeiENSGT00400000022107.
HOVERGENiHBG005344.
InParanoidiQ68FD5.
KOiK04646.
OrthoDBiEOG7Z0JVM.
TreeFamiTF300059.

Enzyme and pathway databases

ReactomeiREACT_299462. WNT5A-dependent internalization of FZD4.
REACT_303468. Lysosome Vesicle Biogenesis.
REACT_316383. MHC class II antigen presentation.
REACT_342454. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiCltc. mouse.
NextBioi324168.
PROiQ68FD5.
SOURCEiSearch...

Gene expression databases

BgeeiQ68FD5.
CleanExiMM_CLTC.
ExpressionAtlasiQ68FD5. baseline and differential.
GenevisibleiQ68FD5. MM.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
2.130.10.110. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016025. Clathrin_H-chain_link/propller.
IPR015348. Clathrin_H-chain_linker_core.
IPR001473. Clathrin_H-chain_propeller_N.
IPR022365. Clathrin_H-chain_propeller_rpt.
IPR016341. Clathrin_heavy_chain.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00637. Clathrin. 7 hits.
PF09268. Clathrin-link. 1 hit.
PF01394. Clathrin_propel. 3 hits.
[Graphical view]
PIRSFiPIRSF002290. Clathrin_H_chain. 1 hit.
SMARTiSM00299. CLH. 7 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 6 hits.
SSF50989. SSF50989. 1 hit.
PROSITEiPS50236. CHCR. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-184; TYR-634; TYR-899; TYR-1206; TYR-1477 AND TYR-1487, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. "Two closely related endocytic proteins that share a common OCRL-binding motif with APPL1."
    Swan L.E., Tomasini L., Pirruccello M., Lunardi J., De Camilli P.
    Proc. Natl. Acad. Sci. U.S.A. 107:3511-3516(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OCRL.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-737 AND LYS-1441, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiCLH1_MOUSE
AccessioniPrimary (citable) accession number: Q68FD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.