Reviewed, UniProtKB/Swiss-Prot Q68F37 (GPAT3_XENLA)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase 3
      Short name=GPAT3
    EC=2.3.1.15
Alternative name(s):
    1-acylglycerol-3-phosphate O-acyltransferase 9
      Short name=1-AGP acyltransferase 9
      Short name=1-AGPAT 9
    Lysophosphatidic acid acyltransferase theta
      Short name=LPAAT-theta

Gene names

Name:	agpat9
Synonyms:	gpat3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis. Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Glycerol-3-phosphate acyltransferase 3		PRO_0000291576
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	168 – 188	21	Potential
	Motif	236 – 241	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q68F37-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 4C10234327F0DD2B	FASTA	446	50,631
	10 20 30 40 50 60 MEDFKTIACN IFIIWLTLVI VLILLPSMFG SSLGISEVYM KILVKVLEWA TLRIQKGFKD 70 80 90 100 110 120 KEKLSASNGI IQRDKSPMET EIECLRQSRS QDIREGDFEL GDVFYFAKKG FEAIVEDEVT 130 140 150 160 170 180 QRFSSEELIS WNLLTRTNNN FHYVSLRVTL IWVLGLCVRY CILLPLRITL ATIGISWLVL 190 200 210 220 230 240 GATLVGQLPN SRMKSWFSEL VHLMCCRICA RALSSAIQYH NKENKPKKGG ICVANHTSPI 250 260 270 280 290 300 DIIILANDGC YAMVGQVHGG LMGIIQRAMA RACPHVWFER SEMRDRHLVT ERLREHVSDK 310 320 330 340 350 360 SKLPILIFPE GTCINNTSVM MFKKGSFEIG GTIYPVAIKY DPQFGDAFWN SSKNSMVSYL 370 380 390 400 410 420 LRMMTSWALK CNVWYLPPVN RQDGEDAVQF ANRVKSAIAK QGGLVELPWD GGLKRGKVKD 430 440 SFKEEQQKNY SRIIAGENKS LKPTTH

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC080008 mRNA. Translation: AAH80008.1.
RefSeq	NP_001087492.1.
UniGene	Xl.48703
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	447316.
KEGG	xla:447316.
Organism-specific databases
Xenbase	XB-FEAT-993434. agpat9.
Phylogenomic databases
HOVERGEN	Q68F37.
Enzyme and pathway databases
BRENDA	2.3.1.51. 648.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT3_XENLA

Accession

Primary (citable) accession number: Q68F37

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 11, 2004
Last modified:	October 13, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project