Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

FAD synthase

Gene

flad1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.By similarity

Catalytic activityi

ATP + FMN = diphosphate + FAD.

Cofactori

Mg2+By similarity

Pathwayi: FAD biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FAD from FMN.
Proteins known to be involved in this subpathway in this organism are:
  1. FAD synthase (flad1)
This subpathway is part of the pathway FAD biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FAD from FMN, the pathway FAD biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DRE-196843 Vitamin B2 (riboflavin) metabolism
UniPathwayiUPA00277; UER00407

Names & Taxonomyi

Protein namesi
Recommended name:
FAD synthase (EC:2.7.7.2)
Alternative name(s):
FAD pyrophosphorylase
FMN adenylyltransferase
Flavin adenine dinucleotide synthase
Including the following 2 domains:
Molybdenum cofactor biosynthesis protein-like region
FAD synthase region
Gene namesi
Name:flad1
ORF Names:zgc:91843
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 16

Organism-specific databases

ZFINiZDB-GENE-040822-44 flad1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003027401 – 497FAD synthaseAdd BLAST497

Proteomic databases

PaxDbiQ68EH8

Expressioni

Gene expression databases

BgeeiENSDARG00000070390
ExpressionAtlasiQ68EH8 baseline

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000094012

Structurei

3D structure databases

ProteinModelPortaliQ68EH8
SMRiQ68EH8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 110Molybdenum cofactor biosynthesis protein-likeAdd BLAST92
Regioni308 – 465FAD synthaseAdd BLAST158

Domaini

The molybdenum cofactor biosynthesis protein-like region may not be functional.

Sequence similaritiesi

In the N-terminal section; belongs to the MoaB/Mog family.Curated
In the C-terminal section; belongs to the PAPS reductase family. FAD1 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2644 Eukaryota
COG0175 LUCA
COG1058 LUCA
GeneTreeiENSGT00390000007266
HOGENOMiHOG000007185
HOVERGENiHBG058211
InParanoidiQ68EH8
KOiK00953
OMAiGQPTYRP
PhylomeDBiQ68EH8
TreeFamiTF314056

Family and domain databases

CDDicd00885 cinA, 1 hit
cd01713 PAPS_reductase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.40.980.10, 1 hit
InterProiView protein in InterPro
IPR012183 FAD_synth_MoaB/Mog-bd
IPR036425 MoaB/Mog-like_dom_sf
IPR001453 MoaB/Mog_dom
IPR002500 PAPS_reduct
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00994 MoCF_biosynth, 1 hit
PF01507 PAPS_reduct, 1 hit
PIRSFiPIRSF036620 MPTbdFAD, 1 hit
SMARTiView protein in SMART
SM00852 MoCF_biosynth, 1 hit
SUPFAMiSSF53218 SSF53218, 1 hit

Sequencei

Sequence statusi: Complete.

Q68EH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNCNTSST QKNGSATAAI LIIGDEILKG HTVDTNSAFL CRGLRKLGIT
60 70 80 90 100
VERITVVPDV QEVIAKEVSQ LSSTVTHLIT SGGIGPTHDD VTFESVAMAF
110 120 130 140 150
GEELYAHPEM TKLVEGFFGT VTSDSAPMKL AMVPASAKLN FGIDPQTGQR
160 170 180 190 200
NRFPLVSVHN VYIFPGIPSL LEKSFNGLSH LFSGSGTTFH TREVFVNADE
210 220 230 240 250
TEIAQSLSKL QAGWGKRVSL GSYPDWLSNY HRVRLVLDTD SVEEVERART
260 270 280 290 300
QLIEELPKGS VVPLVTDPIS VAAQEVYSLS KSETQLGKKV AAALGTIEMA
310 320 330 340 350
LDKYSVNEIC VGFNGGKDCT ALLHLYYAAL KRRYPDGKDR LKALYIRIVS
360 370 380 390 400
PFPEMERFLQ DTIKRYDLEL FSVEGSIRQA LNEVKERRPD LRAVLMGTRR
410 420 430 440 450
TDPYSHTLTP FCPTDPGWPD YMRVNPLLEW TYHDIWSFLR TLYVPYCILY
460 470 480 490
DKGYTSLGSM DNSYRNPSLK MVDERGATRY KPAYMLENEE EERNSRE
Length:497
Mass (Da):55,610
Last modified:October 11, 2004 - v1
Checksum:iD71C34BE8FA99DFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC080254 mRNA Translation: AAH80254.1
RefSeqiNP_001003997.1, NM_001003997.1
UniGeneiDr.76199

Genome annotation databases

EnsembliENSDART00000103235; ENSDARP00000094012; ENSDARG00000070390
GeneIDi445492
KEGGidre:445492

Similar proteinsi

Entry informationi

Entry nameiFAD1_DANRE
AccessioniPrimary (citable) accession number: Q68EH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health