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Protein

Metabotropic glutamate receptor 4

Gene

Grm4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159GlutamateBy similarity1
Binding sitei230GlutamateBy similarity1
Binding sitei312GlutamateBy similarity1
Binding sitei405GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • activation of MAPK activity Source: MGI
  • adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  • chemical synaptic transmission Source: MGI
  • learning Source: MGI
  • regulation of synaptic transmission, glutamatergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 4
Short name:
mGluR4
Gene namesi
Name:Grm4
Synonyms:Gprc1d, Mglur4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1351341. Grm4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 586ExtracellularSequence analysisAdd BLAST554
Transmembranei587 – 607Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini608 – 624CytoplasmicSequence analysisAdd BLAST17
Transmembranei625 – 645Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini646 – 653ExtracellularSequence analysis8
Transmembranei654 – 671Helical; Name=3Sequence analysisAdd BLAST18
Topological domaini672 – 699CytoplasmicSequence analysisAdd BLAST28
Transmembranei700 – 720Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini721 – 751ExtracellularSequence analysisAdd BLAST31
Transmembranei752 – 772Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini773 – 786CytoplasmicSequence analysisAdd BLAST14
Transmembranei787 – 807Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini808 – 826ExtracellularSequence analysisAdd BLAST19
Transmembranei827 – 847Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini848 – 912CytoplasmicSequence analysisAdd BLAST65

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000030685233 – 912Metabotropic glutamate receptor 4Add BLAST880

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 109By similarity
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi249 ↔ 538By similarity
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi372 ↔ 388By similarity
Disulfide bondi428 ↔ 435By similarity
Disulfide bondi520 ↔ 539By similarity
Disulfide bondi524 ↔ 542By similarity
Disulfide bondi545 ↔ 557By similarity
Disulfide bondi560 ↔ 573By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ68EF4.
PeptideAtlasiQ68EF4.
PRIDEiQ68EF4.

PTM databases

iPTMnetiQ68EF4.
PhosphoSitePlusiQ68EF4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000063239.
CleanExiMM_GRM4.

Interactioni

Subunit structurei

Interacts with PICK1.By similarity

Protein-protein interaction databases

BioGridi234582. 1 interactor.
DIPiDIP-49002N.
STRINGi10090.ENSMUSP00000130092.

Structurei

3D structure databases

ProteinModelPortaliQ68EF4.
SMRiQ68EF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 182Glutamate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiQ68EF4.
KOiK04607.
PhylomeDBiQ68EF4.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68EF4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGKGGWAWW WARLPLCLLL SLYGSWVPSS LGKPKGHPHM NSIRIDGDIT
60 70 80 90 100
LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT
110 120 130 140 150
LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV
160 170 180 190 200
VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP
210 220 230 240 250
SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI
260 270 280 290 300
AQSVKIPREP KTGEFDKIIK RLLETSNARA IIIFANEDDI RRVLEAARRA
310 320 330 340 350
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRTS VRGFDRYFSS
360 370 380 390 400
RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY
410 420 430 440 450
EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI
460 470 480 490 500
RNVNFSGIAG NPVTFNENGD APGRYDIYQY QRRNGSAEYK VIGSWTDHLH
510 520 530 540 550
LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY
560 570 580 590 600
QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA
610 620 630 640 650
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG
660 670 680 690 700
TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL
710 720 730 740 750
AITFVLISLQ LLCICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD
760 770 780 790 800
LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA
810 820 830 840 850
FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE
860 870 880 890 900
QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLEAPALAT
910
KQTYVTYTNH AI
Length:912
Mass (Da):101,824
Last modified:October 2, 2007 - v2
Checksum:i5D73C6423CF4E38C
GO
Isoform 2 (identifier: Q68EF4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     815-832: LYIQTTTLTVSVSLSASV → VTSEALPVEFSPPLLAHN
     833-912: Missing.

Show »
Length:832
Mass (Da):92,879
Checksum:i3B94570F77821FB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti713C → G in AAH80284 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028517815 – 832LYIQT…LSASV → VTSEALPVEFSPPLLAHN in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_028518833 – 912Missing in isoform 2. 1 PublicationAdd BLAST80

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072635 mRNA. No translation available.
BC080284 mRNA. Translation: AAH80284.1.
AY255558 mRNA. Translation: AAO85070.1.
CCDSiCCDS28563.1. [Q68EF4-2]
CCDS70775.1. [Q68EF4-1]
RefSeqiNP_001013403.1. NM_001013385.2.
UniGeneiMm.358940.

Genome annotation databases

GeneIDi268934.
KEGGimmu:268934.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072635 mRNA. No translation available.
BC080284 mRNA. Translation: AAH80284.1.
AY255558 mRNA. Translation: AAO85070.1.
CCDSiCCDS28563.1. [Q68EF4-2]
CCDS70775.1. [Q68EF4-1]
RefSeqiNP_001013403.1. NM_001013385.2.
UniGeneiMm.358940.

3D structure databases

ProteinModelPortaliQ68EF4.
SMRiQ68EF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234582. 1 interactor.
DIPiDIP-49002N.
STRINGi10090.ENSMUSP00000130092.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ68EF4.
PhosphoSitePlusiQ68EF4.

Proteomic databases

PaxDbiQ68EF4.
PeptideAtlasiQ68EF4.
PRIDEiQ68EF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi268934.
KEGGimmu:268934.

Organism-specific databases

CTDi2914.
MGIiMGI:1351341. Grm4.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiQ68EF4.
KOiK04607.
PhylomeDBiQ68EF4.

Miscellaneous databases

PROiQ68EF4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063239.
CleanExiMM_GRM4.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRM4_MOUSE
AccessioniPrimary (citable) accession number: Q68EF4
Secondary accession number(s): Q80UC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 2, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.