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Q68EF4

- GRM4_MOUSE

UniProt

Q68EF4 - GRM4_MOUSE

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Protein

Metabotropic glutamate receptor 4

Gene

Grm4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591GlutamateBy similarity
Binding sitei230 – 2301GlutamateBy similarity
Binding sitei312 – 3121GlutamateBy similarity
Binding sitei405 – 4051GlutamateBy similarity

GO - Molecular functioni

  1. glutamate receptor activity Source: UniProtKB
  2. G-protein coupled receptor activity Source: UniProtKB
  3. group III metabotropic glutamate receptor activity Source: RefGenome

GO - Biological processi

  1. activation of MAPK activity Source: Ensembl
  2. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  3. learning Source: MGI
  4. regulation of synaptic transmission, glutamatergic Source: RefGenome
  5. synaptic transmission Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 4
Short name:
mGluR4
Gene namesi
Name:Grm4
Synonyms:Gprc1d, Mglur4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17, UP000000589: Chromosome Y

Organism-specific databases

MGIiMGI:1351341. Grm4.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: Ensembl
  2. dendritic shaft Source: Ensembl
  3. integral component of plasma membrane Source: RefGenome
  4. neuron projection Source: BHF-UCL
  5. presynaptic active zone membrane Source: Ensembl
  6. presynaptic membrane Source: RefGenome
  7. terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 912880Metabotropic glutamate receptor 4PRO_0000306852Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 109By similarity
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi249 ↔ 538By similarity
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi372 ↔ 388By similarity
Disulfide bondi428 ↔ 435By similarity
Disulfide bondi520 ↔ 539By similarity
Disulfide bondi524 ↔ 542By similarity
Disulfide bondi545 ↔ 557By similarity
Disulfide bondi560 ↔ 573By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ68EF4.
PRIDEiQ68EF4.

PTM databases

PhosphoSiteiQ68EF4.

Expressioni

Gene expression databases

BgeeiQ68EF4.
CleanExiMM_GRM4.
ExpressionAtlasiQ68EF4. baseline and differential.
GenevestigatoriQ68EF4.

Interactioni

Subunit structurei

Interacts with PICK1.By similarity

Protein-protein interaction databases

DIPiDIP-49002N.
STRINGi10090.ENSMUSP00000113819.

Structurei

3D structure databases

ProteinModelPortaliQ68EF4.
SMRiQ68EF4. Positions 38-853.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 586554ExtracellularSequence AnalysisAdd
BLAST
Topological domaini608 – 62417CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini646 – 6538ExtracellularSequence Analysis
Topological domaini672 – 69928CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini721 – 75131ExtracellularSequence AnalysisAdd
BLAST
Topological domaini773 – 78614CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini808 – 82619ExtracellularSequence AnalysisAdd
BLAST
Topological domaini848 – 91265CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei587 – 60721Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei625 – 64521Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei654 – 67118Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei700 – 72021Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei752 – 77221Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei787 – 80721Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei827 – 84721Helical; Name=7Sequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 1823Glutamate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiQ68EF4.
KOiK04607.
PhylomeDBiQ68EF4.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q68EF4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGKGGWAWW WARLPLCLLL SLYGSWVPSS LGKPKGHPHM NSIRIDGDIT
60 70 80 90 100
LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT
110 120 130 140 150
LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV
160 170 180 190 200
VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP
210 220 230 240 250
SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI
260 270 280 290 300
AQSVKIPREP KTGEFDKIIK RLLETSNARA IIIFANEDDI RRVLEAARRA
310 320 330 340 350
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRTS VRGFDRYFSS
360 370 380 390 400
RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY
410 420 430 440 450
EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI
460 470 480 490 500
RNVNFSGIAG NPVTFNENGD APGRYDIYQY QRRNGSAEYK VIGSWTDHLH
510 520 530 540 550
LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY
560 570 580 590 600
QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA
610 620 630 640 650
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG
660 670 680 690 700
TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL
710 720 730 740 750
AITFVLISLQ LLCICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD
760 770 780 790 800
LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA
810 820 830 840 850
FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE
860 870 880 890 900
QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLEAPALAT
910
KQTYVTYTNH AI
Length:912
Mass (Da):101,824
Last modified:October 2, 2007 - v2
Checksum:i5D73C6423CF4E38C
GO
Isoform 2 (identifier: Q68EF4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     815-832: LYIQTTTLTVSVSLSASV → VTSEALPVEFSPPLLAHN
     833-912: Missing.

Show »
Length:832
Mass (Da):92,879
Checksum:i3B94570F77821FB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti713 – 7131C → G in AAH80284. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei815 – 83218LYIQT…LSASV → VTSEALPVEFSPPLLAHN in isoform 2. 1 PublicationVSP_028517Add
BLAST
Alternative sequencei833 – 91280Missing in isoform 2. 1 PublicationVSP_028518Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC072635 mRNA. No translation available.
BC080284 mRNA. Translation: AAH80284.1.
AY255558 mRNA. Translation: AAO85070.1.
CCDSiCCDS28563.1. [Q68EF4-2]
CCDS70775.1. [Q68EF4-1]
RefSeqiNP_001013403.1. NM_001013385.2.
UniGeneiMm.358940.

Genome annotation databases

EnsembliENSMUST00000118489; ENSMUSP00000112578; ENSMUSG00000063239.
GeneIDi268934.
KEGGimmu:268934.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC072635 mRNA. No translation available.
BC080284 mRNA. Translation: AAH80284.1 .
AY255558 mRNA. Translation: AAO85070.1 .
CCDSi CCDS28563.1. [Q68EF4-2 ]
CCDS70775.1. [Q68EF4-1 ]
RefSeqi NP_001013403.1. NM_001013385.2.
UniGenei Mm.358940.

3D structure databases

ProteinModelPortali Q68EF4.
SMRi Q68EF4. Positions 38-853.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-49002N.
STRINGi 10090.ENSMUSP00000113819.

Chemistry

GuidetoPHARMACOLOGYi 292.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q68EF4.

Proteomic databases

PaxDbi Q68EF4.
PRIDEi Q68EF4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000118489 ; ENSMUSP00000112578 ; ENSMUSG00000063239 .
GeneIDi 268934.
KEGGi mmu:268934.

Organism-specific databases

CTDi 2914.
MGIi MGI:1351341. Grm4.

Phylogenomic databases

eggNOGi NOG295200.
GeneTreei ENSGT00760000118884.
HOGENOMi HOG000218635.
HOVERGENi HBG107965.
InParanoidi Q68EF4.
KOi K04607.
PhylomeDBi Q68EF4.

Miscellaneous databases

NextBioi 392591.
PROi Q68EF4.
SOURCEi Search...

Gene expression databases

Bgeei Q68EF4.
CleanExi MM_GRM4.
ExpressionAtlasi Q68EF4. baseline and differential.
Genevestigatori Q68EF4.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-642.

Entry informationi

Entry nameiGRM4_MOUSE
AccessioniPrimary (citable) accession number: Q68EF4
Secondary accession number(s): Q80UC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3