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Q68EF4

- GRM4_MOUSE

UniProt

Q68EF4 - GRM4_MOUSE

Protein

Metabotropic glutamate receptor 4

Gene

Grm4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei159 – 1591GlutamateBy similarity
    Binding sitei230 – 2301GlutamateBy similarity
    Binding sitei312 – 3121GlutamateBy similarity
    Binding sitei405 – 4051GlutamateBy similarity

    GO - Molecular functioni

    1. glutamate receptor activity Source: UniProtKB
    2. G-protein coupled receptor activity Source: UniProtKB
    3. group III metabotropic glutamate receptor activity Source: RefGenome

    GO - Biological processi

    1. activation of MAPK activity Source: Ensembl
    2. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
    3. learning Source: MGI
    4. regulation of synaptic transmission, glutamatergic Source: RefGenome
    5. synaptic transmission Source: MGI

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metabotropic glutamate receptor 4
    Short name:
    mGluR4
    Gene namesi
    Name:Grm4
    Synonyms:Gprc1d, Mglur4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17, UP000000589: Chromosome Y

    Organism-specific databases

    MGIiMGI:1351341. Grm4.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: Ensembl
    2. dendritic shaft Source: Ensembl
    3. integral component of plasma membrane Source: RefGenome
    4. neuron projection Source: BHF-UCL
    5. presynaptic active zone membrane Source: Ensembl
    6. presynaptic membrane Source: RefGenome
    7. terminal bouton Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 912880Metabotropic glutamate receptor 4PRO_0000306852Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi67 ↔ 109By similarity
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi249 ↔ 538By similarity
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi372 ↔ 388By similarity
    Disulfide bondi428 ↔ 435By similarity
    Disulfide bondi520 ↔ 539By similarity
    Disulfide bondi524 ↔ 542By similarity
    Disulfide bondi545 ↔ 557By similarity
    Disulfide bondi560 ↔ 573By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ68EF4.
    PRIDEiQ68EF4.

    PTM databases

    PhosphoSiteiQ68EF4.

    Expressioni

    Gene expression databases

    BgeeiQ68EF4.
    CleanExiMM_GRM4.
    GenevestigatoriQ68EF4.

    Interactioni

    Subunit structurei

    Interacts with PICK1.By similarity

    Protein-protein interaction databases

    DIPiDIP-49002N.
    STRINGi10090.ENSMUSP00000113819.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68EF4.
    SMRiQ68EF4. Positions 38-853.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 586554ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini608 – 62417CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini646 – 6538ExtracellularSequence Analysis
    Topological domaini672 – 69928CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini721 – 75131ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini773 – 78614CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini808 – 82619ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini848 – 91265CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei587 – 60721Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei625 – 64521Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei654 – 67118Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei700 – 72021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei752 – 77221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei787 – 80721Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei827 – 84721Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 1823Glutamate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295200.
    GeneTreeiENSGT00750000117581.
    HOGENOMiHOG000218635.
    HOVERGENiHBG107965.
    InParanoidiQ68EF4.
    KOiK04607.
    PhylomeDBiQ68EF4.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001786. GPCR_3_mtglu_rcpt_4.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view]
    PRINTSiPR00248. GPCRMGR.
    PR01054. MTABOTROPC4R.
    PR00593. MTABOTROPICR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q68EF4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGKGGWAWW WARLPLCLLL SLYGSWVPSS LGKPKGHPHM NSIRIDGDIT    50
    LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT 100
    LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV 150
    VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP 200
    SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI 250
    AQSVKIPREP KTGEFDKIIK RLLETSNARA IIIFANEDDI RRVLEAARRA 300
    NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRTS VRGFDRYFSS 350
    RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY 400
    EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI 450
    RNVNFSGIAG NPVTFNENGD APGRYDIYQY QRRNGSAEYK VIGSWTDHLH 500
    LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY 550
    QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA 600
    ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG 650
    TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL 700
    AITFVLISLQ LLCICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD 750
    LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA 800
    FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE 850
    QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLEAPALAT 900
    KQTYVTYTNH AI 912
    Length:912
    Mass (Da):101,824
    Last modified:October 2, 2007 - v2
    Checksum:i5D73C6423CF4E38C
    GO
    Isoform 2 (identifier: Q68EF4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         815-832: LYIQTTTLTVSVSLSASV → VTSEALPVEFSPPLLAHN
         833-912: Missing.

    Show »
    Length:832
    Mass (Da):92,879
    Checksum:i3B94570F77821FB2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti713 – 7131C → G in AAH80284. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei815 – 83218LYIQT…LSASV → VTSEALPVEFSPPLLAHN in isoform 2. 1 PublicationVSP_028517Add
    BLAST
    Alternative sequencei833 – 91280Missing in isoform 2. 1 PublicationVSP_028518Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC072635 mRNA. No translation available.
    BC080284 mRNA. Translation: AAH80284.1.
    AY255558 mRNA. Translation: AAO85070.1.
    CCDSiCCDS28563.1. [Q68EF4-2]
    CCDS70775.1. [Q68EF4-1]
    RefSeqiNP_001013403.1. NM_001013385.2.
    UniGeneiMm.358940.

    Genome annotation databases

    EnsembliENSMUST00000118489; ENSMUSP00000112578; ENSMUSG00000063239.
    GeneIDi268934.
    KEGGimmu:268934.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC072635 mRNA. No translation available.
    BC080284 mRNA. Translation: AAH80284.1 .
    AY255558 mRNA. Translation: AAO85070.1 .
    CCDSi CCDS28563.1. [Q68EF4-2 ]
    CCDS70775.1. [Q68EF4-1 ]
    RefSeqi NP_001013403.1. NM_001013385.2.
    UniGenei Mm.358940.

    3D structure databases

    ProteinModelPortali Q68EF4.
    SMRi Q68EF4. Positions 38-853.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-49002N.
    STRINGi 10090.ENSMUSP00000113819.

    Chemistry

    GuidetoPHARMACOLOGYi 292.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q68EF4.

    Proteomic databases

    PaxDbi Q68EF4.
    PRIDEi Q68EF4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000118489 ; ENSMUSP00000112578 ; ENSMUSG00000063239 .
    GeneIDi 268934.
    KEGGi mmu:268934.

    Organism-specific databases

    CTDi 2914.
    MGIi MGI:1351341. Grm4.

    Phylogenomic databases

    eggNOGi NOG295200.
    GeneTreei ENSGT00750000117581.
    HOGENOMi HOG000218635.
    HOVERGENi HBG107965.
    InParanoidi Q68EF4.
    KOi K04607.
    PhylomeDBi Q68EF4.

    Miscellaneous databases

    NextBioi 392591.
    PROi Q68EF4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q68EF4.
    CleanExi MM_GRM4.
    Genevestigatori Q68EF4.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001786. GPCR_3_mtglu_rcpt_4.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view ]
    PRINTSi PR00248. GPCRMGR.
    PR01054. MTABOTROPC4R.
    PR00593. MTABOTROPICR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-642.

    Entry informationi

    Entry nameiGRM4_MOUSE
    AccessioniPrimary (citable) accession number: Q68EF4
    Secondary accession number(s): Q80UC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3