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Q68EF4 (GRM4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 4

Short name=mGluR4
Gene names
Name:Grm4
Synonyms:Gprc1d, Mglur4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length912 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity By similarity.

Subunit structure

Interacts with PICK1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

learning

Inferred from mutant phenotype PubMed 8815915. Source: MGI

regulation of synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

synaptic transmission

Inferred from mutant phenotype PubMed 8815915. Source: MGI

   Cellular_componentcytoplasmic vesicle

Inferred from electronic annotation. Source: Ensembl

dendritic shaft

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

neuron projection

Inferred from direct assay PubMed 21795692. Source: BHF-UCL

presynaptic active zone membrane

Inferred from electronic annotation. Source: Ensembl

presynaptic membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionG-protein coupled receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

glutamate receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

group III metabotropic glutamate receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q68EF4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q68EF4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     815-832: LYIQTTTLTVSVSLSASV → VTSEALPVEFSPPLLAHN
     833-912: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 912880Metabotropic glutamate receptor 4
PRO_0000306852

Regions

Topological domain33 – 586554Extracellular Potential
Transmembrane587 – 60721Helical; Name=1; Potential
Topological domain608 – 62417Cytoplasmic Potential
Transmembrane625 – 64521Helical; Name=2; Potential
Topological domain646 – 6538Extracellular Potential
Transmembrane654 – 67118Helical; Name=3; Potential
Topological domain672 – 69928Cytoplasmic Potential
Transmembrane700 – 72021Helical; Name=4; Potential
Topological domain721 – 75131Extracellular Potential
Transmembrane752 – 77221Helical; Name=5; Potential
Topological domain773 – 78614Cytoplasmic Potential
Transmembrane787 – 80721Helical; Name=6; Potential
Topological domain808 – 82619Extracellular Potential
Transmembrane827 – 84721Helical; Name=7; Potential
Topological domain848 – 91265Cytoplasmic Potential
Region180 – 1823Glutamate binding By similarity

Sites

Binding site1591Glutamate By similarity
Binding site2301Glutamate By similarity
Binding site3121Glutamate By similarity
Binding site4051Glutamate By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Disulfide bond67 ↔ 109 By similarity
Disulfide bond249 ↔ 538 By similarity
Disulfide bond372 ↔ 388 By similarity
Disulfide bond428 ↔ 435 By similarity
Disulfide bond520 ↔ 539 By similarity
Disulfide bond524 ↔ 542 By similarity
Disulfide bond545 ↔ 557 By similarity
Disulfide bond560 ↔ 573 By similarity

Natural variations

Alternative sequence815 – 83218LYIQT…LSASV → VTSEALPVEFSPPLLAHN in isoform 2.
VSP_028517
Alternative sequence833 – 91280Missing in isoform 2.
VSP_028518

Experimental info

Sequence conflict7131C → G in AAH80284. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 5D73C6423CF4E38C

FASTA912101,824
        10         20         30         40         50         60 
MSGKGGWAWW WARLPLCLLL SLYGSWVPSS LGKPKGHPHM NSIRIDGDIT LGGLFPVHGR 

        70         80         90        100        110        120 
GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT LGARILDTCS RDTHALEQSL 

       130        140        150        160        170        180 
TFVQALIEKD GTEVRCGSGG PPIITKPERV VGVIGASGSS VSIMVANILR LFKIPQISYA 

       190        200        210        220        230        240 
STAPDLSDNS RYDFFSRVVP SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ 

       250        260        270        280        290        300 
KSRENGGVCI AQSVKIPREP KTGEFDKIIK RLLETSNARA IIIFANEDDI RRVLEAARRA 

       310        320        330        340        350        360 
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRTS VRGFDRYFSS RTLDNNRRNI 

       370        380        390        400        410        420 
WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY EQEGKVQFVI DAVYAMGHAL 

       430        440        450        460        470        480 
HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI RNVNFSGIAG NPVTFNENGD APGRYDIYQY 

       490        500        510        520        530        540 
QRRNGSAEYK VIGSWTDHLH LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW 

       550        560        570        580        590        600 
HCEPCTGYQY QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA 

       610        620        630        640        650        660 
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG TCSLRRIFLG 

       670        680        690        700        710        720 
LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL AITFVLISLQ LLCICVWFVV 

       730        740        750        760        770        780 
DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD LSLICLLGYS MLLMVTCTVY AIKTRGVPET 

       790        800        810        820        830        840 
FNEAKPIGFT MYTTCIVWLA FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP 

       850        860        870        880        890        900 
KVYIILFHPE QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLEAPALAT 

       910 
KQTYVTYTNH AI 

« Hide

Isoform 2 [UniParc].

Checksum: 3B94570F77821FB2
Show »

FASTA83292,879

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Brain.
[2]"The G protein-coupled receptor repertoires of human and mouse."
Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E., Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C., Bergmann J.E., Gaitanaris G.A.
Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 528-642.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC072635 mRNA. No translation available.
BC080284 mRNA. Translation: AAH80284.1.
AY255558 mRNA. Translation: AAO85070.1.
CCDSCCDS28563.1. [Q68EF4-2]
RefSeqNP_001013403.1. NM_001013385.2.
UniGeneMm.358940.

3D structure databases

ProteinModelPortalQ68EF4.
SMRQ68EF4. Positions 38-853.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-49002N.
STRING10090.ENSMUSP00000113819.

Chemistry

GuidetoPHARMACOLOGY292.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ68EF4.

Proteomic databases

PaxDbQ68EF4.
PRIDEQ68EF4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000118489; ENSMUSP00000112578; ENSMUSG00000063239.
GeneID268934.
KEGGmmu:268934.

Organism-specific databases

CTD2914.
MGIMGI:1351341. Grm4.

Phylogenomic databases

eggNOGNOG295200.
GeneTreeENSGT00750000117581.
HOGENOMHOG000218635.
HOVERGENHBG107965.
InParanoidQ68EF4.
KOK04607.
PhylomeDBQ68EF4.

Gene expression databases

BgeeQ68EF4.
CleanExMM_GRM4.
GenevestigatorQ68EF4.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio392591.
PROQ68EF4.
SOURCESearch...

Entry information

Entry nameGRM4_MOUSE
AccessionPrimary (citable) accession number: Q68EF4
Secondary accession number(s): Q80UC2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: July 9, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries