Q68DD2 (PA24F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic phospholipase A2 zeta
Short name=cPLA2-zeta
EC=3.1.1.4

Alternative name(s):
Phospholipase A2 group IVF

Gene names

Name:

PLA2G4F

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	849 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Enzyme regulation	Stimulated by cytosolic Ca2+ By similarity.
Subcellular location	Cytoplasm › cytosol. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Translocates to lysosomal membranes in a calcium-dependent fashion By similarity.
Domain	The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 PLA2c domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm Lysosome Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arachidonic acid secretion Inferred from electronic annotation. Source: Compara cellular response to antibiotic Inferred from electronic annotation. Source: Compara cellular response to organic cyclic compound Inferred from electronic annotation. Source: Compara glycerophospholipid biosynthetic process Traceable author statement. Source: Reactome phosphatidylcholine acyl-chain remodeling Traceable author statement. Source: Reactome phosphatidylethanolamine acyl-chain remodeling Traceable author statement. Source: Reactome phosphatidylglycerol acyl-chain remodeling Traceable author statement. Source: Reactome phosphatidylinositol acyl-chain remodeling Traceable author statement. Source: Reactome phosphatidylserine acyl-chain remodeling Traceable author statement. Source: Reactome phospholipid catabolic process Inferred from electronic annotation. Source: InterPro prostaglandin biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Traceable author statement. Source: Reactome lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ruffle membrane Inferred from electronic annotation. Source: Compara vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	calcium-dependent phospholipase A2 activity Inferred from electronic annotation. Source: Compara lysophospholipase activity Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q68DD2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q68DD2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     433-454: ALSTERLQYYTQELGVRERSGH → DVRVSPCQLPRLHSSNLDHSLW
     455-849: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q68DD2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     352-352: G → GQV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 849	849	Cytosolic phospholipase A2 zeta		PRO_0000247027
Regions
	Domain	31 – 133	103	C2
	Domain	306 – 849	544	PLA2c
Sites
	Active site	395	1	Nucleophile By similarity
	Active site	680	1	Proton acceptor By similarity
Natural variations
	Alternative sequence	352	1	G → GQV in isoform 3.		VSP_019887
	Alternative sequence	433 – 454	22	ALSTE…ERSGH → DVRVSPCQLPRLHSSNLDHS LW in isoform 2.		VSP_019888
	Alternative sequence	455 – 849	395	Missing in isoform 2.		VSP_019889
	Natural variant	30	1	G → V. Corresponds to variant rs636604 [ dbSNP | Ensembl ].		VAR_053553
	Natural variant	740	1	M → V. Ref.3 Corresponds to variant rs1356410 [ dbSNP | Ensembl ].		VAR_027054
Experimental info
	Sequence conflict	203	1	R → G in BAD18801. Ref.1
	Sequence conflict	262	1	A → T in BAD18801. Ref.1
	Sequence conflict	363	1	G → E in CAH18288. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: B2231EC3A9494598	FASTA	849	95,082
	10 20 30 40 50 60 MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV LRATNIRGTD 70 80 90 100 110 120 LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY QIHGAVKNVL ELTLYDKDIL 130 140 150 160 170 180 GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ DSQELQVEFV LEKSQVPASE VITNGVLVAH 190 200 210 220 230 240 PCLRIQGTLR GDGTAPREEY GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV 250 260 270 280 290 300 LSSRLHVELM ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA 310 320 330 340 350 360 LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD SGQVPVVAVL 370 380 390 400 410 420 GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC ISTLYRDPAW SQVALQGPIE 430 440 450 460 470 480 RAQVHVCSSK MGALSTERLQ YYTQELGVRE RSGHSVSLID LWGLLVEYLL YQEENPAKLS 490 500 510 520 530 540 DQQEAVRQGQ NPYPIYTSVN VRTNLSGEDF AEWCEFTPYE VGFPKYGAYV PTELFGSELF 550 560 570 580 590 600 MGRLLQLQPE PRICYLQGMW GSAFATSLDE IFLKTAGSGL SFLEWYRGSV NITDDCQKPQ 610 620 630 640 650 660 LHNPSRLRTR LLTPQGPFSQ AVLDIFTSRF TSAQSFNFTR GLCLHKDYVA GREFVAWKDT 670 680 690 700 710 720 HPDAFPNQLT PMRDCLYLVD GGFAINSPFP LALLPQRAVD LILSFDYSLE APFEVLKMTE 730 740 750 760 770 780 KYCLDRGIPF PSIEVGPEDM EEARECYLFA KAEDPRSPIV LHFPLVNRTF RTHLAPGVER 790 800 810 820 830 840 QTAEEKAFGD FVINRPDTPY GMMNFTYEPQ DFYRLVALSR YNVLNNVETL KCALQLALDR HQARERAGA
	Isoform 2 [UniParc]. Checksum: 4EFC69C84A65CCD7	FASTA	454	50,003
	10 20 30 40 50 60 MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV LRATNIRGTD 70 80 90 100 110 120 LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY QIHGAVKNVL ELTLYDKDIL 130 140 150 160 170 180 GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ DSQELQVEFV LEKSQVPASE VITNGVLVAH 190 200 210 220 230 240 PCLRIQGTLR GDGTAPREEY GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV 250 260 270 280 290 300 LSSRLHVELM ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA 310 320 330 340 350 360 LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD SGQVPVVAVL 370 380 390 400 410 420 GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC ISTLYRDPAW SQVALQGPIE 430 440 450 RAQVHVCSSK MGDVRVSPCQ LPRLHSSNLD HSLW
	Isoform 3 [UniParc]. Checksum: 0367FEB0244B6293	FASTA	851	95,310
	10 20 30 40 50 60 MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV LRATNIRGTD 70 80 90 100 110 120 LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY QIHGAVKNVL ELTLYDKDIL 130 140 150 160 170 180 GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ DSQELQVEFV LEKSQVPASE VITNGVLVAH 190 200 210 220 230 240 PCLRIQGTLR GDGTAPREEY GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV 250 260 270 280 290 300 LSSRLHVELM ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA 310 320 330 340 350 360 LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD SGQVQVPVVA 370 380 390 400 410 420 VLGSGGGTRA MSSLYGSLAG LQELGLLDTV TYLSGVSGST WCISTLYRDP AWSQVALQGP 430 440 450 460 470 480 IERAQVHVCS SKMGALSTER LQYYTQELGV RERSGHSVSL IDLWGLLVEY LLYQEENPAK 490 500 510 520 530 540 LSDQQEAVRQ GQNPYPIYTS VNVRTNLSGE DFAEWCEFTP YEVGFPKYGA YVPTELFGSE 550 560 570 580 590 600 LFMGRLLQLQ PEPRICYLQG MWGSAFATSL DEIFLKTAGS GLSFLEWYRG SVNITDDCQK 610 620 630 640 650 660 PQLHNPSRLR TRLLTPQGPF SQAVLDIFTS RFTSAQSFNF TRGLCLHKDY VAGREFVAWK 670 680 690 700 710 720 DTHPDAFPNQ LTPMRDCLYL VDGGFAINSP FPLALLPQRA VDLILSFDYS LEAPFEVLKM 730 740 750 760 770 780 TEKYCLDRGI PFPSIEVGPE DMEEARECYL FAKAEDPRSP IVLHFPLVNR TFRTHLAPGV 790 800 810 820 830 840 ERQTAEEKAF GDFVINRPDT PYGMMNFTYE PQDFYRLVAL SRYNVLNNVE TLKCALQLAL 850 DRHQARERAG A

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Small intestine.
[2]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 201-849 (ISOFORM 3), VARIANT VAL-740. Tissue: Fetal kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK172836 mRNA. Translation: BAD18801.1. AC036103 Genomic DNA. No translation available. CR749451 mRNA. Translation: CAH18288.1.
IPI	IPI00743154. IPI00783797. IPI00940945.
RefSeq	NP_998765.3. NM_213600.3.
UniGene	Hs.231873.
3D structure databases
ProteinModelPortal	Q68DD2.
SMR	Q68DD2. Positions 44-162, 232-834.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q68DD2. 1 interaction.
STRING	9606.ENSP00000371833.
PTM databases
PhosphoSite	Q68DD2.
Polymorphism databases
DMDM	110815895.
Proteomic databases
PaxDb	Q68DD2.
PRIDE	Q68DD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000382396; ENSP00000371833; ENSG00000168907.
GeneID	255189.
KEGG	hsa:255189.
UCSC	uc001zoy.3. human.
Organism-specific databases
CTD	255189.
GeneCards	GC15M042433.
H-InvDB	HIX0202165.
HGNC	HGNC:27396. PLA2G4F.
HPA	HPA042713.
neXtProt	NX_Q68DD2.
PharmGKB	PA142671170.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG300348.
HOGENOM	HOG000231788.
HOVERGEN	HBG080412.
KO	K16342.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q68DD2.
Bgee	Q68DD2.
CleanEx	HS_PLA2G4F.
Genevestigator	Q68DD2.
GermOnline	ENSG00000168907. Homo sapiens.
Family and domain databases
InterPro	IPR016035. Acyl_Trfase/lysoPLipase. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR002642. LysoPLipase_cat_dom. [Graphical view]
Pfam	PF00168. C2. 1 hit. PF01735. PLA2_B. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. SM00022. PLAc. 1 hit. [Graphical view]
SUPFAM	SSF52151. Acyl_Trfase/lysoPlipase. 1 hit. SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS51210. PLA2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	255189.
NextBio	92510.

Entry information

Entry name

PA24F_HUMAN

Accession

Primary (citable) accession number: Q68DD2
Secondary accession number(s): Q6ZMC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.