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Protein

Cytosolic phospholipase A2 zeta

Gene

PLA2G4F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei395 – 3951NucleophileBy similarity
Active sitei680 – 6801Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_120722. Acyl chain remodelling of PI.
REACT_120829. Acyl chain remodelling of PC.
REACT_120977. Hydrolysis of LPC.
REACT_121324. Acyl chain remodelling of PG.
REACT_121369. Acyl chain remodelling of PE.
REACT_121384. Acyl chain remodelling of PS.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 zeta (EC:3.1.1.4)
Short name:
cPLA2-zeta
Alternative name(s):
Phospholipase A2 group IVF
Gene namesi
Name:PLA2G4F
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:27396. PLA2G4F.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671170.

Polymorphism and mutation databases

BioMutaiPLA2G4F.
DMDMi317373489.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 849849Cytosolic phospholipase A2 zetaPRO_0000247027Add
BLAST

Proteomic databases

MaxQBiQ68DD2.
PaxDbiQ68DD2.
PRIDEiQ68DD2.

PTM databases

PhosphoSiteiQ68DD2.

Expressioni

Gene expression databases

BgeeiQ68DD2.
CleanExiHS_PLA2G4F.
ExpressionAtlasiQ68DD2. baseline and differential.
GenevisibleiQ68DD2. HS.

Organism-specific databases

HPAiHPA056752.

Interactioni

Protein-protein interaction databases

BioGridi129083. 1 interaction.
IntActiQ68DD2. 1 interaction.
STRINGi9606.ENSP00000371833.

Structurei

3D structure databases

ProteinModelPortaliQ68DD2.
SMRiQ68DD2. Positions 44-162, 232-834.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 133103C2PROSITE-ProRule annotationAdd
BLAST
Domaini306 – 849544PLA2cPROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG300348.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ68DD2.
KOiK16342.
OMAiGMWGSAF.
OrthoDBiEOG7JX33K.
PhylomeDBiQ68DD2.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68DD2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV
60 70 80 90 100
LRATNIRGTD LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY
110 120 130 140 150
QIHGAVKNVL ELTLYDKDIL GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ
160 170 180 190 200
DSQELQVEFV LEKSQVPASE VITNGVLVAH PCLRIQGTLR GDGTAPREEY
210 220 230 240 250
GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV LSSRLHVELM
260 270 280 290 300
ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA
310 320 330 340 350
LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD
360 370 380 390 400
SGQVPVVAVL GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC
410 420 430 440 450
ISTLYRDPAW SQVALQGPIE RAQVHVCSSK MGALSTERLQ YYTQELGVRE
460 470 480 490 500
RSGHSVSLID LWGLLVEYLL YQEENPAKLS DQQEAVRQGQ NPYPIYTSVN
510 520 530 540 550
VRTNLSGEDF AEWCEFTPYE VGFPKYGAYV PTELFGSELF MGRLLQLQPE
560 570 580 590 600
PRICYLQGMW GSAFATSLDE IFLKTAGSGL SFLEWYRGSV NITDDCQKPQ
610 620 630 640 650
LHNPSRLRTR LLTPQGPFSQ AVLDIFTSRF TSAQSFNFTR GLCLHKDYVA
660 670 680 690 700
GREFVAWKDT HPDAFPNQLT PMRDCLYLVD GGFAINSPFP LALLPQRAVD
710 720 730 740 750
LILSFDYSLE APFEVLKMTE KYCLDRGIPF PSIEVGPEDM EEARECYLFA
760 770 780 790 800
KAEDPRSPIV LHFPLVNRTF RTHLAPGVER QTAEEKAFGD FVINRPDTPY
810 820 830 840
GMMNFTYEPQ DFYRLVALSR YNVLNNVETL KCALQLALDR HQARERAGA
Length:849
Mass (Da):95,082
Last modified:January 11, 2011 - v3
Checksum:iB2231EC3A9494598
GO
Isoform 2 (identifier: Q68DD2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-454: ALSTERLQYYTQELGVRERSGH → DVRVSPCQLPRLHSSNLDHSLW
     455-849: Missing.

Note: No experimental confirmation available.
Show »
Length:454
Mass (Da):50,003
Checksum:i4EFC69C84A65CCD7
GO
Isoform 3 (identifier: Q68DD2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     352-352: G → GQV

Note: No experimental confirmation available.
Show »
Length:851
Mass (Da):95,310
Checksum:i0367FEB0244B6293
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031R → G in BAD18801 (PubMed:14702039).Curated
Sequence conflicti262 – 2621A → T in BAD18801 (PubMed:14702039).Curated
Sequence conflicti363 – 3631G → E in CAH18288 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301G → V.
Corresponds to variant rs636604 [ dbSNP | Ensembl ].
VAR_053553
Natural varianti740 – 7401M → V.1 Publication
Corresponds to variant rs1356410 [ dbSNP | Ensembl ].
VAR_027054

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei352 – 3521G → GQV in isoform 3. 1 PublicationVSP_019887
Alternative sequencei433 – 45422ALSTE…ERSGH → DVRVSPCQLPRLHSSNLDHS LW in isoform 2. 1 PublicationVSP_019888Add
BLAST
Alternative sequencei455 – 849395Missing in isoform 2. 1 PublicationVSP_019889Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172836 mRNA. Translation: BAD18801.1.
AC036103 Genomic DNA. No translation available.
CR749451 mRNA. Translation: CAH18288.1.
CCDSiCCDS32204.1. [Q68DD2-1]
RefSeqiNP_998765.3. NM_213600.3. [Q68DD2-1]
UniGeneiHs.231873.

Genome annotation databases

EnsembliENST00000397272; ENSP00000380442; ENSG00000168907.
GeneIDi255189.
KEGGihsa:255189.
UCSCiuc001zoy.3. human. [Q68DD2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172836 mRNA. Translation: BAD18801.1.
AC036103 Genomic DNA. No translation available.
CR749451 mRNA. Translation: CAH18288.1.
CCDSiCCDS32204.1. [Q68DD2-1]
RefSeqiNP_998765.3. NM_213600.3. [Q68DD2-1]
UniGeneiHs.231873.

3D structure databases

ProteinModelPortaliQ68DD2.
SMRiQ68DD2. Positions 44-162, 232-834.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129083. 1 interaction.
IntActiQ68DD2. 1 interaction.
STRINGi9606.ENSP00000371833.

PTM databases

PhosphoSiteiQ68DD2.

Polymorphism and mutation databases

BioMutaiPLA2G4F.
DMDMi317373489.

Proteomic databases

MaxQBiQ68DD2.
PaxDbiQ68DD2.
PRIDEiQ68DD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397272; ENSP00000380442; ENSG00000168907.
GeneIDi255189.
KEGGihsa:255189.
UCSCiuc001zoy.3. human. [Q68DD2-1]

Organism-specific databases

CTDi255189.
GeneCardsiGC15M042433.
H-InvDBHIX0202165.
HGNCiHGNC:27396. PLA2G4F.
HPAiHPA056752.
neXtProtiNX_Q68DD2.
PharmGKBiPA142671170.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG300348.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ68DD2.
KOiK16342.
OMAiGMWGSAF.
OrthoDBiEOG7JX33K.
PhylomeDBiQ68DD2.
TreeFamiTF325228.

Enzyme and pathway databases

ReactomeiREACT_120722. Acyl chain remodelling of PI.
REACT_120829. Acyl chain remodelling of PC.
REACT_120977. Hydrolysis of LPC.
REACT_121324. Acyl chain remodelling of PG.
REACT_121369. Acyl chain remodelling of PE.
REACT_121384. Acyl chain remodelling of PS.

Miscellaneous databases

GenomeRNAii255189.
NextBioi92510.
PROiQ68DD2.

Gene expression databases

BgeeiQ68DD2.
CleanExiHS_PLA2G4F.
ExpressionAtlasiQ68DD2. baseline and differential.
GenevisibleiQ68DD2. HS.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Small intestine.
  2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 201-849 (ISOFORM 3), VARIANT VAL-740.
    Tissue: Fetal kidney.

Entry informationi

Entry nameiPA24F_HUMAN
AccessioniPrimary (citable) accession number: Q68DD2
Secondary accession number(s): Q6ZMC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 11, 2011
Last modified: July 22, 2015
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.