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Protein

Cytosolic phospholipase A2 zeta

Gene

PLA2G4F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei395NucleophileBy similarity1
Active sitei680Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1482788 Acyl chain remodelling of PC
R-HSA-1482801 Acyl chain remodelling of PS
R-HSA-1482839 Acyl chain remodelling of PE
R-HSA-1482922 Acyl chain remodelling of PI
R-HSA-1482925 Acyl chain remodelling of PG
R-HSA-1483115 Hydrolysis of LPC

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 zeta (EC:3.1.1.4)
Short name:
cPLA2-zeta
Alternative name(s):
Phospholipase A2 group IVF
Gene namesi
Name:PLA2G4F
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000168907.13
HGNCiHGNC:27396 PLA2G4F
neXtProtiNX_Q68DD2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000168907
PharmGKBiPA142671170

Polymorphism and mutation databases

BioMutaiPLA2G4F
DMDMi317373489

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470271 – 849Cytosolic phospholipase A2 zetaAdd BLAST849

Proteomic databases

MaxQBiQ68DD2
PaxDbiQ68DD2
PeptideAtlasiQ68DD2
PRIDEiQ68DD2

PTM databases

iPTMnetiQ68DD2
PhosphoSitePlusiQ68DD2

Expressioni

Gene expression databases

BgeeiENSG00000168907
CleanExiHS_PLA2G4F
ExpressionAtlasiQ68DD2 baseline and differential
GenevisibleiQ68DD2 HS

Organism-specific databases

HPAiHPA042713
HPA056752

Interactioni

Protein-protein interaction databases

BioGridi129083, 1 interactor
IntActiQ68DD2, 1 interactor
STRINGi9606.ENSP00000371833

Structurei

3D structure databases

ProteinModelPortaliQ68DD2
SMRiQ68DD2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 133C2PROSITE-ProRule annotationAdd BLAST103
Domaini306 – 849PLA2cPROSITE-ProRule annotationAdd BLAST544

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
KOG1325 Eukaryota
ENOG410XR72 LUCA
GeneTreeiENSGT00550000074489
HOGENOMiHOG000231788
HOVERGENiHBG080412
InParanoidiQ68DD2
KOiK16342
OMAiDFAEWCE
OrthoDBiEOG091G01HN
PhylomeDBiQ68DD2
TreeFamiTF325228

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF01735 PLA2_B, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS51210 PLA2C, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q68DD2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV
60 70 80 90 100
LRATNIRGTD LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY
110 120 130 140 150
QIHGAVKNVL ELTLYDKDIL GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ
160 170 180 190 200
DSQELQVEFV LEKSQVPASE VITNGVLVAH PCLRIQGTLR GDGTAPREEY
210 220 230 240 250
GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV LSSRLHVELM
260 270 280 290 300
ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA
310 320 330 340 350
LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD
360 370 380 390 400
SGQVPVVAVL GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC
410 420 430 440 450
ISTLYRDPAW SQVALQGPIE RAQVHVCSSK MGALSTERLQ YYTQELGVRE
460 470 480 490 500
RSGHSVSLID LWGLLVEYLL YQEENPAKLS DQQEAVRQGQ NPYPIYTSVN
510 520 530 540 550
VRTNLSGEDF AEWCEFTPYE VGFPKYGAYV PTELFGSELF MGRLLQLQPE
560 570 580 590 600
PRICYLQGMW GSAFATSLDE IFLKTAGSGL SFLEWYRGSV NITDDCQKPQ
610 620 630 640 650
LHNPSRLRTR LLTPQGPFSQ AVLDIFTSRF TSAQSFNFTR GLCLHKDYVA
660 670 680 690 700
GREFVAWKDT HPDAFPNQLT PMRDCLYLVD GGFAINSPFP LALLPQRAVD
710 720 730 740 750
LILSFDYSLE APFEVLKMTE KYCLDRGIPF PSIEVGPEDM EEARECYLFA
760 770 780 790 800
KAEDPRSPIV LHFPLVNRTF RTHLAPGVER QTAEEKAFGD FVINRPDTPY
810 820 830 840
GMMNFTYEPQ DFYRLVALSR YNVLNNVETL KCALQLALDR HQARERAGA
Length:849
Mass (Da):95,082
Last modified:January 11, 2011 - v3
Checksum:iB2231EC3A9494598
GO
Isoform 2 (identifier: Q68DD2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-454: ALSTERLQYYTQELGVRERSGH → DVRVSPCQLPRLHSSNLDHSLW
     455-849: Missing.

Note: No experimental confirmation available.
Show »
Length:454
Mass (Da):50,003
Checksum:i4EFC69C84A65CCD7
GO
Isoform 3 (identifier: Q68DD2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     352-352: G → GQV

Note: No experimental confirmation available.
Show »
Length:851
Mass (Da):95,310
Checksum:i0367FEB0244B6293
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203R → G in BAD18801 (PubMed:14702039).Curated1
Sequence conflicti262A → T in BAD18801 (PubMed:14702039).Curated1
Sequence conflicti363G → E in CAH18288 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05355330G → V. Corresponds to variant dbSNP:rs636604Ensembl.1
Natural variantiVAR_027054740M → V1 PublicationCorresponds to variant dbSNP:rs1356410Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019887352G → GQV in isoform 3. 1 Publication1
Alternative sequenceiVSP_019888433 – 454ALSTE…ERSGH → DVRVSPCQLPRLHSSNLDHS LW in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_019889455 – 849Missing in isoform 2. 1 PublicationAdd BLAST395

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172836 mRNA Translation: BAD18801.1
AC036103 Genomic DNA No translation available.
CR749451 mRNA Translation: CAH18288.1
CCDSiCCDS32204.1 [Q68DD2-1]
RefSeqiNP_998765.3, NM_213600.3 [Q68DD2-1]
UniGeneiHs.231873

Genome annotation databases

EnsembliENST00000397272; ENSP00000380442; ENSG00000168907 [Q68DD2-1]
GeneIDi255189
KEGGihsa:255189
UCSCiuc001zoz.4 human [Q68DD2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPA24F_HUMAN
AccessioniPrimary (citable) accession number: Q68DD2
Secondary accession number(s): Q6ZMC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 11, 2011
Last modified: March 28, 2018
This is version 108 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health