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Q68DD2

- PA24F_HUMAN

UniProt

Q68DD2 - PA24F_HUMAN

Protein

Cytosolic phospholipase A2 zeta

Gene

PLA2G4F

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 3 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine By similarity.By similarity

    Catalytic activityi

    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Stimulated by cytosolic Ca2+.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei395 – 3951NucleophileBy similarity
    Active sitei680 – 6801Proton acceptorBy similarity

    GO - Molecular functioni

    1. calcium-dependent phospholipase A2 activity Source: Ensembl
    2. lysophospholipase activity Source: Ensembl
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. arachidonic acid secretion Source: Ensembl
    2. cellular response to antibiotic Source: Ensembl
    3. cellular response to organic cyclic compound Source: Ensembl
    4. glycerophospholipid biosynthetic process Source: Reactome
    5. phosphatidylcholine acyl-chain remodeling Source: Reactome
    6. phosphatidylethanolamine acyl-chain remodeling Source: Reactome
    7. phosphatidylglycerol acyl-chain remodeling Source: Reactome
    8. phosphatidylinositol acyl-chain remodeling Source: Reactome
    9. phosphatidylserine acyl-chain remodeling Source: Reactome
    10. phospholipid catabolic process Source: InterPro
    11. phospholipid metabolic process Source: Reactome
    12. prostaglandin biosynthetic process Source: Ensembl
    13. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_120722. Acyl chain remodelling of PI.
    REACT_120829. Acyl chain remodelling of PC.
    REACT_120977. Hydrolysis of LPC.
    REACT_121324. Acyl chain remodelling of PG.
    REACT_121369. Acyl chain remodelling of PE.
    REACT_121384. Acyl chain remodelling of PS.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic phospholipase A2 zeta (EC:3.1.1.4)
    Short name:
    cPLA2-zeta
    Alternative name(s):
    Phospholipase A2 group IVF
    Gene namesi
    Name:PLA2G4F
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:27396. PLA2G4F.

    Subcellular locationi

    Cytoplasmcytosol. Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Translocates to lysosomal membranes in a calcium-dependent fashion.By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. lysosomal membrane Source: UniProtKB-SubCell
    3. ruffle membrane Source: Ensembl
    4. vesicle Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Lysosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142671170.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 849849Cytosolic phospholipase A2 zetaPRO_0000247027Add
    BLAST

    Proteomic databases

    MaxQBiQ68DD2.
    PaxDbiQ68DD2.
    PRIDEiQ68DD2.

    PTM databases

    PhosphoSiteiQ68DD2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ68DD2.
    BgeeiQ68DD2.
    CleanExiHS_PLA2G4F.
    GenevestigatoriQ68DD2.

    Organism-specific databases

    HPAiHPA042713.

    Interactioni

    Protein-protein interaction databases

    BioGridi129083. 1 interaction.
    IntActiQ68DD2. 1 interaction.
    STRINGi9606.ENSP00000371833.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68DD2.
    SMRiQ68DD2. Positions 44-162, 232-834.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 133103C2PROSITE-ProRule annotationAdd
    BLAST
    Domaini306 – 849544PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG300348.
    HOGENOMiHOG000231788.
    HOVERGENiHBG080412.
    KOiK16342.
    OrthoDBiEOG7JX33K.
    PhylomeDBiQ68DD2.
    TreeFamiTF325228.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q68DD2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV    50
    LRATNIRGTD LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY 100
    QIHGAVKNVL ELTLYDKDIL GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ 150
    DSQELQVEFV LEKSQVPASE VITNGVLVAH PCLRIQGTLR GDGTAPREEY 200
    GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV LSSRLHVELM 250
    ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA 300
    LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD 350
    SGQVPVVAVL GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC 400
    ISTLYRDPAW SQVALQGPIE RAQVHVCSSK MGALSTERLQ YYTQELGVRE 450
    RSGHSVSLID LWGLLVEYLL YQEENPAKLS DQQEAVRQGQ NPYPIYTSVN 500
    VRTNLSGEDF AEWCEFTPYE VGFPKYGAYV PTELFGSELF MGRLLQLQPE 550
    PRICYLQGMW GSAFATSLDE IFLKTAGSGL SFLEWYRGSV NITDDCQKPQ 600
    LHNPSRLRTR LLTPQGPFSQ AVLDIFTSRF TSAQSFNFTR GLCLHKDYVA 650
    GREFVAWKDT HPDAFPNQLT PMRDCLYLVD GGFAINSPFP LALLPQRAVD 700
    LILSFDYSLE APFEVLKMTE KYCLDRGIPF PSIEVGPEDM EEARECYLFA 750
    KAEDPRSPIV LHFPLVNRTF RTHLAPGVER QTAEEKAFGD FVINRPDTPY 800
    GMMNFTYEPQ DFYRLVALSR YNVLNNVETL KCALQLALDR HQARERAGA 849
    Length:849
    Mass (Da):95,082
    Last modified:January 11, 2011 - v3
    Checksum:iB2231EC3A9494598
    GO
    Isoform 2 (identifier: Q68DD2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         433-454: ALSTERLQYYTQELGVRERSGH → DVRVSPCQLPRLHSSNLDHSLW
         455-849: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:454
    Mass (Da):50,003
    Checksum:i4EFC69C84A65CCD7
    GO
    Isoform 3 (identifier: Q68DD2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         352-352: G → GQV

    Note: No experimental confirmation available.

    Show »
    Length:851
    Mass (Da):95,310
    Checksum:i0367FEB0244B6293
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti203 – 2031R → G in BAD18801. (PubMed:14702039)Curated
    Sequence conflicti262 – 2621A → T in BAD18801. (PubMed:14702039)Curated
    Sequence conflicti363 – 3631G → E in CAH18288. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti30 – 301G → V.
    Corresponds to variant rs636604 [ dbSNP | Ensembl ].
    VAR_053553
    Natural varianti740 – 7401M → V.1 Publication
    Corresponds to variant rs1356410 [ dbSNP | Ensembl ].
    VAR_027054

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei352 – 3521G → GQV in isoform 3. 1 PublicationVSP_019887
    Alternative sequencei433 – 45422ALSTE…ERSGH → DVRVSPCQLPRLHSSNLDHS LW in isoform 2. 1 PublicationVSP_019888Add
    BLAST
    Alternative sequencei455 – 849395Missing in isoform 2. 1 PublicationVSP_019889Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK172836 mRNA. Translation: BAD18801.1.
    AC036103 Genomic DNA. No translation available.
    CR749451 mRNA. Translation: CAH18288.1.
    CCDSiCCDS32204.1. [Q68DD2-1]
    RefSeqiNP_998765.3. NM_213600.3. [Q68DD2-1]
    UniGeneiHs.231873.

    Genome annotation databases

    EnsembliENST00000382396; ENSP00000371833; ENSG00000168907. [Q68DD2-1]
    GeneIDi255189.
    KEGGihsa:255189.
    UCSCiuc001zoy.3. human. [Q68DD2-1]

    Polymorphism databases

    DMDMi317373489.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK172836 mRNA. Translation: BAD18801.1 .
    AC036103 Genomic DNA. No translation available.
    CR749451 mRNA. Translation: CAH18288.1 .
    CCDSi CCDS32204.1. [Q68DD2-1 ]
    RefSeqi NP_998765.3. NM_213600.3. [Q68DD2-1 ]
    UniGenei Hs.231873.

    3D structure databases

    ProteinModelPortali Q68DD2.
    SMRi Q68DD2. Positions 44-162, 232-834.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129083. 1 interaction.
    IntActi Q68DD2. 1 interaction.
    STRINGi 9606.ENSP00000371833.

    PTM databases

    PhosphoSitei Q68DD2.

    Polymorphism databases

    DMDMi 317373489.

    Proteomic databases

    MaxQBi Q68DD2.
    PaxDbi Q68DD2.
    PRIDEi Q68DD2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000382396 ; ENSP00000371833 ; ENSG00000168907 . [Q68DD2-1 ]
    GeneIDi 255189.
    KEGGi hsa:255189.
    UCSCi uc001zoy.3. human. [Q68DD2-1 ]

    Organism-specific databases

    CTDi 255189.
    GeneCardsi GC15M042433.
    H-InvDB HIX0202165.
    HGNCi HGNC:27396. PLA2G4F.
    HPAi HPA042713.
    neXtProti NX_Q68DD2.
    PharmGKBi PA142671170.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG300348.
    HOGENOMi HOG000231788.
    HOVERGENi HBG080412.
    KOi K16342.
    OrthoDBi EOG7JX33K.
    PhylomeDBi Q68DD2.
    TreeFami TF325228.

    Enzyme and pathway databases

    Reactomei REACT_120722. Acyl chain remodelling of PI.
    REACT_120829. Acyl chain remodelling of PC.
    REACT_120977. Hydrolysis of LPC.
    REACT_121324. Acyl chain remodelling of PG.
    REACT_121369. Acyl chain remodelling of PE.
    REACT_121384. Acyl chain remodelling of PS.

    Miscellaneous databases

    GenomeRNAii 255189.
    NextBioi 92510.
    PROi Q68DD2.

    Gene expression databases

    ArrayExpressi Q68DD2.
    Bgeei Q68DD2.
    CleanExi HS_PLA2G4F.
    Genevestigatori Q68DD2.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR000008. C2_dom.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF52151. SSF52151. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Small intestine.
    2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 201-849 (ISOFORM 3), VARIANT VAL-740.
      Tissue: Fetal kidney.

    Entry informationi

    Entry nameiPA24F_HUMAN
    AccessioniPrimary (citable) accession number: Q68DD2
    Secondary accession number(s): Q6ZMC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 87 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3