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Q68DD2 (PA24F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic phospholipase A2 zeta

Short name=cPLA2-zeta
EC=3.1.1.4
Alternative name(s):
Phospholipase A2 group IVF
Gene names
Name:PLA2G4F
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length849 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine By similarity.

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation

Stimulated by cytosolic Ca2+ By similarity.

Subcellular location

Cytoplasmcytosol. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Translocates to lysosomal membranes in a calcium-dependent fashion By similarity.

Domain

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PLA2c domain.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentCytoplasm
Lysosome
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarachidonic acid secretion

Inferred from electronic annotation. Source: Ensembl

cellular response to antibiotic

Inferred from electronic annotation. Source: Ensembl

cellular response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

glycerophospholipid biosynthetic process

Traceable author statement. Source: Reactome

phosphatidylcholine acyl-chain remodeling

Traceable author statement. Source: Reactome

phosphatidylethanolamine acyl-chain remodeling

Traceable author statement. Source: Reactome

phosphatidylglycerol acyl-chain remodeling

Traceable author statement. Source: Reactome

phosphatidylinositol acyl-chain remodeling

Traceable author statement. Source: Reactome

phosphatidylserine acyl-chain remodeling

Traceable author statement. Source: Reactome

phospholipid catabolic process

Inferred from electronic annotation. Source: InterPro

phospholipid metabolic process

Traceable author statement. Source: Reactome

prostaglandin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

lysosomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

ruffle membrane

Inferred from electronic annotation. Source: Ensembl

vesicle

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncalcium-dependent phospholipase A2 activity

Inferred from electronic annotation. Source: Ensembl

lysophospholipase activity

Inferred from electronic annotation. Source: Ensembl

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q68DD2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q68DD2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     433-454: ALSTERLQYYTQELGVRERSGH → DVRVSPCQLPRLHSSNLDHSLW
     455-849: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q68DD2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     352-352: G → GQV
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 849849Cytosolic phospholipase A2 zeta
PRO_0000247027

Regions

Domain31 – 133103C2
Domain306 – 849544PLA2c

Sites

Active site3951Nucleophile By similarity
Active site6801Proton acceptor By similarity

Natural variations

Alternative sequence3521G → GQV in isoform 3.
VSP_019887
Alternative sequence433 – 45422ALSTE…ERSGH → DVRVSPCQLPRLHSSNLDHS LW in isoform 2.
VSP_019888
Alternative sequence455 – 849395Missing in isoform 2.
VSP_019889
Natural variant301G → V.
Corresponds to variant rs636604 [ dbSNP | Ensembl ].
VAR_053553
Natural variant7401M → V. Ref.3
Corresponds to variant rs1356410 [ dbSNP | Ensembl ].
VAR_027054

Experimental info

Sequence conflict2031R → G in BAD18801. Ref.1
Sequence conflict2621A → T in BAD18801. Ref.1
Sequence conflict3631G → E in CAH18288. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 3.
Checksum: B2231EC3A9494598

FASTA84995,082
        10         20         30         40         50         60 
MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV LRATNIRGTD 

        70         80         90        100        110        120 
LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY QIHGAVKNVL ELTLYDKDIL 

       130        140        150        160        170        180 
GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ DSQELQVEFV LEKSQVPASE VITNGVLVAH 

       190        200        210        220        230        240 
PCLRIQGTLR GDGTAPREEY GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV 

       250        260        270        280        290        300 
LSSRLHVELM ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA 

       310        320        330        340        350        360 
LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD SGQVPVVAVL 

       370        380        390        400        410        420 
GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC ISTLYRDPAW SQVALQGPIE 

       430        440        450        460        470        480 
RAQVHVCSSK MGALSTERLQ YYTQELGVRE RSGHSVSLID LWGLLVEYLL YQEENPAKLS 

       490        500        510        520        530        540 
DQQEAVRQGQ NPYPIYTSVN VRTNLSGEDF AEWCEFTPYE VGFPKYGAYV PTELFGSELF 

       550        560        570        580        590        600 
MGRLLQLQPE PRICYLQGMW GSAFATSLDE IFLKTAGSGL SFLEWYRGSV NITDDCQKPQ 

       610        620        630        640        650        660 
LHNPSRLRTR LLTPQGPFSQ AVLDIFTSRF TSAQSFNFTR GLCLHKDYVA GREFVAWKDT 

       670        680        690        700        710        720 
HPDAFPNQLT PMRDCLYLVD GGFAINSPFP LALLPQRAVD LILSFDYSLE APFEVLKMTE 

       730        740        750        760        770        780 
KYCLDRGIPF PSIEVGPEDM EEARECYLFA KAEDPRSPIV LHFPLVNRTF RTHLAPGVER 

       790        800        810        820        830        840 
QTAEEKAFGD FVINRPDTPY GMMNFTYEPQ DFYRLVALSR YNVLNNVETL KCALQLALDR 


HQARERAGA 

« Hide

Isoform 2 [UniParc].

Checksum: 4EFC69C84A65CCD7
Show »

FASTA45450,003
Isoform 3 [UniParc].

Checksum: 0367FEB0244B6293
Show »

FASTA85195,310

References

[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Small intestine.
[2]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 201-849 (ISOFORM 3), VARIANT VAL-740.
Tissue: Fetal kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK172836 mRNA. Translation: BAD18801.1.
AC036103 Genomic DNA. No translation available.
CR749451 mRNA. Translation: CAH18288.1.
CCDSCCDS32204.1. [Q68DD2-1]
RefSeqNP_998765.3. NM_213600.3. [Q68DD2-1]
UniGeneHs.231873.

3D structure databases

ProteinModelPortalQ68DD2.
SMRQ68DD2. Positions 44-162, 232-834.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129083. 1 interaction.
IntActQ68DD2. 1 interaction.
STRING9606.ENSP00000371833.

PTM databases

PhosphoSiteQ68DD2.

Polymorphism databases

DMDM317373489.

Proteomic databases

MaxQBQ68DD2.
PaxDbQ68DD2.
PRIDEQ68DD2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000382396; ENSP00000371833; ENSG00000168907. [Q68DD2-1]
GeneID255189.
KEGGhsa:255189.
UCSCuc001zoy.3. human. [Q68DD2-1]

Organism-specific databases

CTD255189.
GeneCardsGC15M042433.
H-InvDBHIX0202165.
HGNCHGNC:27396. PLA2G4F.
HPAHPA042713.
neXtProtNX_Q68DD2.
PharmGKBPA142671170.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG300348.
HOGENOMHOG000231788.
HOVERGENHBG080412.
KOK16342.
OrthoDBEOG7JX33K.
PhylomeDBQ68DD2.
TreeFamTF325228.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressQ68DD2.
BgeeQ68DD2.
CleanExHS_PLA2G4F.
GenevestigatorQ68DD2.

Family and domain databases

Gene3D2.60.40.150. 1 hit.
InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi255189.
NextBio92510.
PROQ68DD2.

Entry information

Entry namePA24F_HUMAN
AccessionPrimary (citable) accession number: Q68DD2
Secondary accession number(s): Q6ZMC8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM