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Protein

Natural cytotoxicity triggering receptor 3 ligand 1

Gene

NCR3LG1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Triggers NCR3-dependent natural killer cell activation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
Natural cytotoxicity triggering receptor 3 ligand 1
Alternative name(s):
B7 homolog 6
Short name:
B7-H6
Gene namesi
Name:NCR3LG1
Synonyms:B7H6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:42400. NCR3LG1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 262238ExtracellularSequence analysisAdd
BLAST
Transmembranei263 – 28321HelicalSequence analysisAdd
BLAST
Topological domaini284 – 454171CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Polymorphism and mutation databases

BioMutaiNCR3LG1.
DMDMi74708829.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 454430Natural cytotoxicity triggering receptor 3 ligand 1PRO_0000390564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...)1 Publication
Disulfide bondi48 ↔ 122PROSITE-ProRule annotation1 Publication
Glycosylationi57 – 571N-linked (GlcNAc...)1 Publication
Disulfide bondi163 ↔ 228PROSITE-ProRule annotation1 Publication
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)1 Publication
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence analysis
Glycosylationi242 – 2421N-linked (GlcNAc...)1 Publication
Glycosylationi260 – 2601N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ68D85.
MaxQBiQ68D85.
PaxDbiQ68D85.
PRIDEiQ68D85.

PTM databases

iPTMnetiQ68D85.
PhosphoSiteiQ68D85.
UniCarbKBiQ68D85.

Expressioni

Tissue specificityi

Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).1 Publication

Gene expression databases

BgeeiQ68D85.
GenevisibleiQ68D85. HS.

Organism-specific databases

HPAiHPA024137.

Interactioni

Subunit structurei

Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1.2 Publications

Protein-protein interaction databases

BioGridi131895. 36 interactions.
DIPiDIP-59940N.
STRINGi9606.ENSP00000341637.

Structurei

Secondary structure

1
454
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 294Combined sources
Beta strandi34 – 396Combined sources
Beta strandi44 – 463Combined sources
Beta strandi49 – 546Combined sources
Helixi58 – 603Combined sources
Beta strandi62 – 687Combined sources
Beta strandi70 – 734Combined sources
Beta strandi75 – 8410Combined sources
Beta strandi86 – 905Combined sources
Helixi97 – 1004Combined sources
Turni101 – 1033Combined sources
Beta strandi107 – 1115Combined sources
Helixi114 – 1163Combined sources
Beta strandi118 – 1269Combined sources
Beta strandi129 – 14113Combined sources
Beta strandi144 – 1485Combined sources
Beta strandi160 – 17112Combined sources
Beta strandi174 – 1807Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi194 – 2018Combined sources
Beta strandi207 – 2148Combined sources
Beta strandi225 – 2317Combined sources
Beta strandi239 – 2435Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PV6X-ray2.30A25-262[»]
3PV7X-ray2.00A25-262[»]
ProteinModelPortaliQ68D85.
SMRiQ68D85. Positions 24-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ68D85.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 138112Ig-like V-typeAdd
BLAST
Domaini143 – 244102Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 624Interaction with NCR3
Regioni127 – 1304Interaction with NCR3
Regioni291 – 429139Retroviral-Gag-likeAdd
BLAST

Domaini

The C-terminal part is similar to retroviral Gag protein. This putative protein seems to be the result of a fusion between an Ig-like domain-containing protein and a ERV.

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKZM. Eukaryota.
ENOG4110NPT. LUCA.
GeneTreeiENSGT00660000095644.
HOVERGENiHBG057683.
InParanoidiQ68D85.
KOiK16349.
OMAiNINTIQQ.
OrthoDBiEOG7MSMPT.
PhylomeDBiQ68D85.
TreeFamiTF331083.

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR000840. G_retro_matrix.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR010999. Retrovr_matrix.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF01140. Gag_MA. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68D85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWRAAASTC AALLILLWAL TTEGDLKVEM MAGGTQITPL NDNVTIFCNI
60 70 80 90 100
FYSQPLNITS MGITWFWKSL TFDKEVKVFE FFGDHQEAFR PGAIVSPWRL
110 120 130 140 150
KSGDASLRLP GIQLEEAGEY RCEVVVTPLK AQGTVQLEVV ASPASRLLLD
160 170 180 190 200
QVGMKENEDK YMCESSGFYP EAINITWEKQ TQKFPHPIEI SEDVITGPTI
210 220 230 240 250
KNMDGTFNVT SCLKLNSSQE DPGTVYQCVV RHASLHTPLR SNFTLTAARH
260 270 280 290 300
SLSETEKTDN FSIHWWPISF IGVGLVLLIV LIPWKKICNK SSSAYTPLKC
310 320 330 340 350
ILKHWNSFDT QTLKKEHLIF FCTRAWPSYQ LQDGEAWPPE GSVNINTIQQ
360 370 380 390 400
LDVFCRQEGK WSEVPYVQAF FALRDNPDLC QCCRIDPALL TVTSGKSIDD
410 420 430 440 450
NSTKSEKQTP REHSDAVPDA PILPVSPIWE PPPATTSTTP VLSSQPPTLL

LPLQ
Length:454
Mass (Da):50,827
Last modified:October 11, 2004 - v1
Checksum:i433CE0B0EE0A4470
GO

Sequence cautioni

The sequence CAD97811.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371I → V in CAD97811 (PubMed:17974005).Curated
Sequence conflicti211 – 2111S → N in CAD97811 (PubMed:17974005).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX537685 mRNA. Translation: CAD97811.1. Different initiation.
CR749521 mRNA. Translation: CAH18335.1.
AC124798 Genomic DNA. No translation available.
BC136800 mRNA. No translation available.
BC136797 mRNA. No translation available.
CCDSiCCDS55748.1.
RefSeqiNP_001189368.1. NM_001202439.2.
UniGeneiHs.146274.

Genome annotation databases

EnsembliENST00000338965; ENSP00000341637; ENSG00000188211.
ENST00000530403; ENSP00000434394; ENSG00000188211.
GeneIDi374383.
KEGGihsa:374383.
UCSCiuc001mmz.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX537685 mRNA. Translation: CAD97811.1. Different initiation.
CR749521 mRNA. Translation: CAH18335.1.
AC124798 Genomic DNA. No translation available.
BC136800 mRNA. No translation available.
BC136797 mRNA. No translation available.
CCDSiCCDS55748.1.
RefSeqiNP_001189368.1. NM_001202439.2.
UniGeneiHs.146274.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PV6X-ray2.30A25-262[»]
3PV7X-ray2.00A25-262[»]
ProteinModelPortaliQ68D85.
SMRiQ68D85. Positions 24-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131895. 36 interactions.
DIPiDIP-59940N.
STRINGi9606.ENSP00000341637.

PTM databases

iPTMnetiQ68D85.
PhosphoSiteiQ68D85.
UniCarbKBiQ68D85.

Polymorphism and mutation databases

BioMutaiNCR3LG1.
DMDMi74708829.

Proteomic databases

EPDiQ68D85.
MaxQBiQ68D85.
PaxDbiQ68D85.
PRIDEiQ68D85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338965; ENSP00000341637; ENSG00000188211.
ENST00000530403; ENSP00000434394; ENSG00000188211.
GeneIDi374383.
KEGGihsa:374383.
UCSCiuc001mmz.5. human.

Organism-specific databases

CTDi374383.
GeneCardsiNCR3LG1.
HGNCiHGNC:42400. NCR3LG1.
HPAiHPA024137.
MIMi613714. gene.
neXtProtiNX_Q68D85.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKZM. Eukaryota.
ENOG4110NPT. LUCA.
GeneTreeiENSGT00660000095644.
HOVERGENiHBG057683.
InParanoidiQ68D85.
KOiK16349.
OMAiNINTIQQ.
OrthoDBiEOG7MSMPT.
PhylomeDBiQ68D85.
TreeFamiTF331083.

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTraceiQ68D85.
GenomeRNAii374383.
NextBioi100166.
PROiQ68D85.
SOURCEiSearch...

Gene expression databases

BgeeiQ68D85.
GenevisibleiQ68D85. HS.

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR000840. G_retro_matrix.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR010999. Retrovr_matrix.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF01140. Gag_MA. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterine endothelium.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cerebellum.
  4. "The B7 family member B7-H6 is a tumor cell ligand for the activating natural killer cell receptor NKp30 in humans."
    Brandt C.S., Baratin M., Yi E.C., Kennedy J., Gao Z., Fox B., Haldeman B., Ostrander C.D., Kaifu T., Chabannon C., Moretta A., West R., Xu W., Vivier E., Levin S.D.
    J. Exp. Med. 206:1495-1503(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NCR3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Structure of the human activating natural cytotoxicity receptor NKp30 bound to its tumor cell ligand B7-H6."
    Li Y., Wang Q., Mariuzza R.A.
    J. Exp. Med. 208:703-714(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-262 ALONE AND IN COMPLEX WITH NCR3, GLYCOSYLATION AT ASN-43; ASN-57; ASN-208 AND ASN-242, SUBUNIT, DISULFIDE BONDS.

Entry informationi

Entry nameiNR3L1_HUMAN
AccessioniPrimary (citable) accession number: Q68D85
Secondary accession number(s): Q7Z3M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.