ID   SLN13_HUMAN             Reviewed;         897 AA.
AC   Q68D06; E1P645; Q658M1; Q6ZS51; Q96A81;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   24-JAN-2024, entry version 137.
DE   RecName: Full=Schlafen family member 13;
DE            EC=3.1.-.- {ECO:0000269|PubMed:29563550};
DE   AltName: Full=Schlafen-13 {ECO:0000305};
DE            Short=hSLFN13 {ECO:0000303|PubMed:29563550};
GN   Name=SLFN13 {ECO:0000312|HGNC:HGNC:26481};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Cervix, and Stomach;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, ACTIVE SITE, AND MUTAGENESIS OF GLU-208;
RP   GLU-213 AND ASP-251.
RX   PubMed=29563550; DOI=10.1038/s41467-018-03544-x;
RA   Yang J.Y., Deng X.Y., Li Y.S., Ma X.C., Feng J.X., Yu B., Chen Y.,
RA   Luo Y.L., Wang X., Chen M.L., Fang Z.X., Zheng F.X., Li Y.P., Zhong Q.,
RA   Kang T.B., Song L.B., Xu R.H., Zeng M.S., Chen W., Zhang H., Xie W.,
RA   Gao S.;
RT   "Structure of Schlafen13 reveals a new class of tRNA/rRNA- targeting RNase
RT   engaged in translational control.";
RL   Nat. Commun. 9:1165-1165(2018).
CC   -!- FUNCTION: Endoribonuclease that cleaves tRNAs and rRNAs
CC       (PubMed:29563550). Cleaves tRNAs 11 nucleotides from the 3'-terminus at
CC       the acceptor stem (PubMed:29563550). Does not act on tRNA(Sec)
CC       (PubMed:29563550). Able to restrict HIV-1 virus replication; ability to
CC       inhibit HIV-1 replication is dependent on endoribonuclease activity
CC       (PubMed:29563550). {ECO:0000269|PubMed:29563550}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q5U311};
CC       Note=Can also use Mn(2+). {ECO:0000250|UniProtKB:Q5U311};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29563550}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q68D06-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q68D06-2; Sequence=VSP_024273;
CC   -!- DOMAIN: Shows a pseudo-dimeric U-pillow-shaped architecture of the
CC       SLFN13 N'-domain that may clamp base-paired RNAs.
CC       {ECO:0000250|UniProtKB:Q5U311}.
CC   -!- SIMILARITY: Belongs to the Schlafen family. Subgroup III subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AK127728; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AK056514; BAB71201.1; -; mRNA.
DR   EMBL; AK127728; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; CR749630; CAH18424.1; -; mRNA.
DR   EMBL; AL833747; CAH56227.1; -; mRNA.
DR   EMBL; CH471147; EAW80150.1; -; Genomic_DNA.
DR   EMBL; CH471147; EAW80152.1; -; Genomic_DNA.
DR   CCDS; CCDS32620.1; -. [Q68D06-1]
DR   RefSeq; NP_653283.3; NM_144682.5. [Q68D06-1]
DR   RefSeq; XP_005257979.1; XM_005257922.2. [Q68D06-1]
DR   RefSeq; XP_016879721.1; XM_017024232.1.
DR   RefSeq; XP_016879722.1; XM_017024233.1. [Q68D06-1]
DR   AlphaFoldDB; Q68D06; -.
DR   SMR; Q68D06; -.
DR   BioGRID; 127019; 4.
DR   STRING; 9606.ENSP00000285013; -.
DR   iPTMnet; Q68D06; -.
DR   PhosphoSitePlus; Q68D06; -.
DR   BioMuta; SLFN13; -.
DR   DMDM; 74708791; -.
DR   EPD; Q68D06; -.
DR   jPOST; Q68D06; -.
DR   MassIVE; Q68D06; -.
DR   MaxQB; Q68D06; -.
DR   PaxDb; 9606-ENSP00000285013; -.
DR   PeptideAtlas; Q68D06; -.
DR   ProteomicsDB; 66041; -. [Q68D06-1]
DR   ProteomicsDB; 66042; -. [Q68D06-2]
DR   Pumba; Q68D06; -.
DR   Antibodypedia; 15604; 78 antibodies from 16 providers.
DR   DNASU; 146857; -.
DR   Ensembl; ENST00000285013.11; ENSP00000285013.6; ENSG00000154760.14. [Q68D06-1]
DR   Ensembl; ENST00000526861.5; ENSP00000434439.1; ENSG00000154760.14. [Q68D06-1]
DR   Ensembl; ENST00000533791.5; ENSP00000467426.1; ENSG00000154760.14. [Q68D06-1]
DR   Ensembl; ENST00000534689.5; ENSP00000435442.1; ENSG00000154760.14. [Q68D06-2]
DR   Ensembl; ENST00000542635.5; ENSP00000444016.1; ENSG00000154760.14. [Q68D06-1]
DR   GeneID; 146857; -.
DR   KEGG; hsa:146857; -.
DR   MANE-Select; ENST00000285013.11; ENSP00000285013.6; NM_144682.6; NP_653283.3.
DR   UCSC; uc002hjk.2; human. [Q68D06-1]
DR   AGR; HGNC:26481; -.
DR   CTD; 146857; -.
DR   DisGeNET; 146857; -.
DR   GeneCards; SLFN13; -.
DR   HGNC; HGNC:26481; SLFN13.
DR   HPA; ENSG00000154760; Tissue enhanced (bone marrow, salivary gland).
DR   MIM; 614957; gene.
DR   neXtProt; NX_Q68D06; -.
DR   OpenTargets; ENSG00000154760; -.
DR   PharmGKB; PA144596360; -.
DR   VEuPathDB; HostDB:ENSG00000154760; -.
DR   eggNOG; ENOG502QWKG; Eukaryota.
DR   GeneTree; ENSGT00410000025651; -.
DR   HOGENOM; CLU_007071_0_0_1; -.
DR   InParanoid; Q68D06; -.
DR   OMA; TKQQGSC; -.
DR   OrthoDB; 5398472at2759; -.
DR   PhylomeDB; Q68D06; -.
DR   TreeFam; TF337168; -.
DR   PathwayCommons; Q68D06; -.
DR   BioGRID-ORCS; 146857; 9 hits in 1148 CRISPR screens.
DR   ChiTaRS; SLFN13; human.
DR   GenomeRNAi; 146857; -.
DR   Pharos; Q68D06; Tdark.
DR   PRO; PR:Q68D06; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; Q68D06; Protein.
DR   Bgee; ENSG00000154760; Expressed in right uterine tube and 116 other cell types or tissues.
DR   ExpressionAtlas; Q68D06; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004521; F:RNA endonuclease activity; IDA:UniProtKB.
DR   GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR   GO; GO:0051607; P:defense response to virus; IBA:GO_Central.
DR   GO; GO:0016075; P:rRNA catabolic process; IDA:UniProtKB.
DR   GO; GO:0016078; P:tRNA catabolic process; IDA:UniProtKB.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   Gene3D; 3.30.950.30; Schlafen, AAA domain; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR031450; Poxin-SLFN/SLFN_N.
DR   InterPro; IPR029684; Schlafen.
DR   InterPro; IPR007421; Schlafen_AlbA_2_dom.
DR   InterPro; IPR038461; Schlafen_AlbA_2_dom_sf.
DR   InterPro; IPR018647; SLFN_3-like_DNA/RNA_helicase.
DR   InterPro; IPR048729; SLFN_GTPase-like.
DR   PANTHER; PTHR12155; SCHLAFEN; 1.
DR   PANTHER; PTHR12155:SF43; SCHLAFEN FAMILY MEMBER 13; 1.
DR   Pfam; PF17057; B3R; 1.
DR   Pfam; PF09848; SLFN-g3_helicase; 1.
DR   Pfam; PF04326; SLFN_AlbA_2; 1.
DR   Pfam; PF21026; SLFN_GTPase-like; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   Genevisible; Q68D06; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Cytoplasm; Endonuclease; Hydrolase;
KW   Magnesium; Metal-binding; Nuclease; Nucleotide-binding; Reference proteome;
KW   Zinc.
FT   CHAIN           1..897
FT                   /note="Schlafen family member 13"
FT                   /id="PRO_0000282985"
FT   REGION          2..355
FT                   /note="N'-domain region"
FT                   /evidence="ECO:0000250|UniProtKB:Q5U311"
FT   ACT_SITE        208
FT                   /evidence="ECO:0000269|PubMed:29563550"
FT   ACT_SITE        213
FT                   /evidence="ECO:0000269|PubMed:29563550"
FT   BINDING         284
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q5U311"
FT   BINDING         286
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q5U311"
FT   BINDING         321
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q5U311"
FT   BINDING         599..606
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         9..326
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_024273"
FT   VARIANT         4
FT                   /note="N -> S (in dbSNP:rs12943866)"
FT                   /id="VAR_031449"
FT   VARIANT         50
FT                   /note="A -> T (in dbSNP:rs7216628)"
FT                   /id="VAR_031450"
FT   VARIANT         433
FT                   /note="P -> L (in dbSNP:rs16970912)"
FT                   /id="VAR_053878"
FT   VARIANT         642
FT                   /note="D -> V (in dbSNP:rs11657183)"
FT                   /id="VAR_053879"
FT   VARIANT         652
FT                   /note="E -> K (in dbSNP:rs3744371)"
FT                   /id="VAR_031451"
FT   MUTAGEN         208
FT                   /note="E->A: Abolished endoribonuclease activity."
FT                   /evidence="ECO:0000269|PubMed:29563550"
FT   MUTAGEN         213
FT                   /note="E->A: Abolished endoribonuclease activity."
FT                   /evidence="ECO:0000269|PubMed:29563550"
FT   MUTAGEN         251
FT                   /note="D->A: Reduced endoribonuclease activity."
FT                   /evidence="ECO:0000269|PubMed:29563550"
SQ   SEQUENCE   897 AA;  102045 MW;  2D516123F44121D0 CRC64;
     MEANHCSLGV YPSYPDLVID VGEVTLGEEN RKKLQKTQRD QERARVIRAA CALLNSGGGV
     IQMEMANRDE RPTEMGLDLE ESLRKLIQYP YLQAFFETKQ HGRCFYIFVK SWSGDPFLKD
     GSFNSRICSL SSSLYCRSGT SVLHMNSRQA FDFLKTKERQ SKYNLINEGS PPSKIMKAVY
     QNISESNPAY EVFQTDTIEY GEILSFPESP SIEFKQFSTK HIQQYVENII PEYISAFANT
     EGGYLFIGVD DKSRKVLGCA KEQVDPDSLK NVIARAISKL PIVHFCSSKP RVEYSTKIVE
     VFCGKELYGY LCVIKVKAFC CVVFSEAPKS WMVREKYIRP LTTEEWVEKM MDADPEFPPD
     FAEAFESQLS LSDSPSLCRP VYSKKGLEHK ADLQQHLFPV PPGHLECTPE SLWKELSLQH
     EGLKELIHKQ MRPFSQGIVI LSRSWAVDLN LQEKPGVICD ALLIAQNSTP ILYTILREQD
     AEGQDYCTRT AFTLKQKLVN MGGYTGKVCV RAKVLCLSPE SSAEALEAAV SPMDYPASYS
     LAGTQHMEAL LQSLVIVLLG FRSLLSDQLG CEVLNLLTAQ QYEIFSRSLR KNRELFVHGL
     PGSGKTIMAM KIMEKIRNVF HCEAHRILYV CENQPLRNFI SDRNICRAET RETFLREKFE
     HIQHIVIDEA QNFRTEDGDW YRKAKTITQR EKDCPGVLWI FLDYFQTSHL GHSGLPPLSA
     QYPREELTRV VRNADEIAEY IQQEMQLIIE NPPINIPHGY LAILSEAKWV PGVPGNTKII
     KNFTLEQIVT YVADTCRCFF ERGYSPKDVA VLVSTVTEVE QYQSKLLKAM RKKMVVQLSD
     ACDMLGVHIV LDSVRRFSGL ERSIVFGIHP RTADPAILPN ILICLASRAK QHLYIFL
//