Reviewed, UniProtKB/Swiss-Prot Q68CK6 (ACS2B_HUMAN)

Names and origin

Protein names

Recommended name:
    Acyl-coenzyme A synthetase ACSM2B, mitochondrial
    EC=6.2.1.2
Alternative name(s):
    Acyl-CoA synthetase medium-chain family member 2B
    Middle-chain acyl-CoA synthetase 2B
    Butyryl coenzyme A synthetase 2B
    Butyrate--CoA ligase 2B
    Xenobiotic/medium-chain fatty acid-CoA ligase HXM-A

Gene names

Name:	ACSM2B
Synonyms:	ACSM2
ORF Names:	HYST1046

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). Ref.1
Catalytic activity	ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium or manganese. Ref.3
Enzyme regulation	Activated by monovalent cations, such as potassium, rubidium or ammonium. Ref.3
Subunit structure	Monomer. Ref.1
Subcellular location	Mitochondrion matrix Ref.1 Ref.3 Ref.4.
Tissue specificity	Detected in liver. Ref.3 Ref.4
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW butyrate-CoA ligase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Mitochondrion Potential
	Chain	47 – 577	531	Acyl-coenzyme A synthetase ACSM2B, mitochondrial		PRO_0000306094
Regions
	Nucleotide binding	221 – 229	9	ATP By similarity
	Nucleotide binding	359 – 364	6	ATP By similarity
	Region	469 – 471	3	Coenzyme A binding By similarity
	Region	540 – 542	3	Coenzyme A binding By similarity
Sites
	Binding site	139	1	Coenzyme A By similarity
	Binding site	364	1	Substrate By similarity
	Binding site	446	1	ATP By similarity
	Binding site	461	1	ATP By similarity
	Binding site	472	1	Substrate By similarity
	Binding site	501	1	Coenzyme A By similarity
	Binding site	532	1	Coenzyme A By similarity
	Binding site	557	1	ATP By similarity
Experimental info
	Sequence conflict	46	1	F → L in AAO17576. Ref.1
	Sequence conflict	133	1	F → I in AAO17576. Ref.1
	Sequence conflict	136	1	G → E in AAO17576. Ref.1
	Sequence conflict	143	1	T → A in AAO17576. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q68CK6-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B95F55B1EE721BD6	FASTA	577	64,257
	10 20 30 40 50 60 MHWLRKVQGL CTLWGTQMSS RTLYINSRQL VSLQWGHQEV PAKFNFASDV LDHWADMEKA 70 80 90 100 110 120 GKRLPSPALW WVNGKGKELM WNFRELSENS QQAANVLSGA CGLQRGDRVA VMLPRVPEWW 130 140 150 160 170 180 LVILGCIRAG LIFMPGTIQM KSTDILYRLQ MSKAKAIVAG DEVIQEVDTV ASECPSLRIK 190 200 210 220 230 240 LLVSEKSCDG WLNFKKLLNE ASTTHHCVET GSQEASAIYF TSGTSGLPKM AEHSYSSLGL 250 260 270 280 290 300 KAKMDAGWTG LQASDIMWTI SDTGWILNIL GSLLESWTLG ACTFVHLLPK FDPLVILKTL 310 320 330 340 350 360 SSYPIKSMMG APIVYRMLLQ QDLSSYKFPH LQNCLAGGES LLPETLENWR AQTGLDIREF 370 380 390 400 410 420 YGQTETGLTC MVSKTMKIKP GYMGTAASCY DVQVIDDKGN VLPPGTEGDI GIRVKPIRPI 430 440 450 460 470 480 GIFSGYVENP DKTAANIRGD FWLLGDRGIK DEDGYFQFMG RADDIINSSG YRIGPSEVEN 490 500 510 520 530 540 ALMKHPAVVE TAVISSPDPV RGEVVKAFVI LASQFLSHDP EQLTKELQQH VKSVTAPYKY 550 560 570 PRKIEFVLNL PKTVTGKIQR TKLRDKEWKM SGKARAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, sequencing, and expression of a cDNA for the HXM-A form of xenobiotic/medium-chain fatty acid:CoA ligase from human liver mitochondria." Vessey D.A., Lau E., Kelley M., Warren R.S. J. Biochem. Mol. Toxicol. 17:1-6(2003) [PubMed: 12616642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 152-163 AND 483-491, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT. Tissue: Liver.
[2]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed: 15221005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[3]	"Characterization of the CoA ligases of human liver mitochondria catalyzing the activation of short- and medium-chain fatty acids and xenobiotic carboxylic acids." Vessey D.A., Kelley M., Warren R.S. Biochim. Biophys. Acta 1428:455-462(1999) [PubMed: 10434065] [Abstract] Cited for: SUBCELLULAR LOCATION, COFACTOR, TISSUE SPECIFICITY, ENZYME REGULATION.
[4]	"Comparative analyses of disease risk genes belonging to the acyl-CoA synthetase medium-chain (ACSM) family in human liver and cell lines." Boomgaarden I., Vock C., Klapper M., Doering F. Biochem. Genet. 47:739-748(2009) [PubMed: 19634011] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY160217 mRNA. Translation: AAO17576.1. AB073604 mRNA. Translation: BAD38642.1.
IPI	IPI00329444.
RefSeq	NP_872423.3.
UniGene	Hs.298252 Hs.567879
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
SMR	Q68CK6. Positions 33-569.
ModBase	Search...
Protein-protein interaction databases
STRING	Q68CK6.
PTM databases
PhosphoSite	Q68CK6.
Proteomic databases
PRIDE	Q68CK6.
Genome annotation databases
Ensembl	ENST00000329697; ENSP00000327453; ENSG00000066813; Homo sapiens. [Genome view] ENST00000414188; ENSP00000390378; ENSG00000066813; Homo sapiens. [Genome view]
GeneID	348158.
UCSC	uc002dhj.2. human.
Organism-specific databases
CTD	348158.
GeneCards	GC16M020457.
HGNC	HGNC:30931. ACSM2B.
PharmGKB	PA142672650.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10263.
HOGENOM	HBG547964.
HOVERGEN	Q68CK6.
InParanoid	Q68CK6.
Enzyme and pathway databases
BRENDA	6.2.1.2. 247.
Reactome	REACT_13433. Biological oxidations.
Gene expression databases
ArrayExpress	Q68CK6.
Bgee	Q68CK6.
CleanEx	HS_ACSM2B.
Genevestigator	Q68CK6.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	99351.

Entry information

Entry name

ACS2B_HUMAN

Accession

Primary (citable) accession number: Q68CK6
Secondary accession number(s): Q86YT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 11, 2004
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.