Reviewed,
UniProtKB/Swiss-Prot Q68CK6 (ACS2B_HUMAN)
Last modified
January 19, 2010.
Version 46.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Acyl-coenzyme A synthetase ACSM2B, mitochondrial EC=6.2.1.2 Alternative name(s): Acyl-CoA synthetase medium-chain family member 2B Middle-chain acyl-CoA synthetase 2B Butyryl coenzyme A synthetase 2B Butyrate--CoA ligase 2B Xenobiotic/medium-chain fatty acid-CoA ligase HXM-A | ||||||
| Gene names |
| ||||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 577 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). Ref.1 |
| Catalytic activity | ATP + an acid + CoA = AMP + diphosphate + an acyl-CoA. |
| Cofactor | Magnesium or manganese. Ref.3 |
| Enzyme regulation | Activated by monovalent cations, such as potassium, rubidium or ammonium. Ref.3 |
| Subunit structure | Monomer. Ref.1 |
| Subcellular location | |
| Tissue specificity | |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Fatty acid metabolism Lipid metabolism |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW butyrate-CoA ligase activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 46 | 46 | Mitochondrion Potential | ||||||
| Chain | 47 – 577 | 531 | Acyl-coenzyme A synthetase ACSM2B, mitochondrial | PRO_0000306094 | |||||
Regions | |||||||||
| Nucleotide binding | 221 – 229 | 9 | ATP By similarity | ||||||
| Nucleotide binding | 359 – 364 | 6 | ATP By similarity | ||||||
| Region | 469 – 471 | 3 | Coenzyme A binding By similarity | ||||||
| Region | 540 – 542 | 3 | Coenzyme A binding By similarity | ||||||
Sites | |||||||||
| Binding site | 139 | 1 | Coenzyme A By similarity | ||||||
| Binding site | 364 | 1 | Substrate By similarity | ||||||
| Binding site | 446 | 1 | ATP By similarity | ||||||
| Binding site | 461 | 1 | ATP By similarity | ||||||
| Binding site | 472 | 1 | Substrate By similarity | ||||||
| Binding site | 501 | 1 | Coenzyme A By similarity | ||||||
| Binding site | 532 | 1 | Coenzyme A By similarity | ||||||
| Binding site | 557 | 1 | ATP By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 46 | 1 | F → L in AAO17576. Ref.1 | ||||||
| Sequence conflict | 133 | 1 | F → I in AAO17576. Ref.1 | ||||||
| Sequence conflict | 136 | 1 | G → E in AAO17576. Ref.1 | ||||||
| Sequence conflict | 143 | 1 | T → A in AAO17576. Ref.1 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Isolation, sequencing, and expression of a cDNA for the HXM-A form of xenobiotic/medium-chain fatty acid:CoA ligase from human liver mitochondria." Vessey D.A., Lau E., Kelley M., Warren R.S. J. Biochem. Mol. Toxicol. 17:1-6(2003) [PubMed: 12616642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 152-163 AND 483-491, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT. Tissue: Liver. |
| [2] | "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed: 15221005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver. |
| [3] | "Characterization of the CoA ligases of human liver mitochondria catalyzing the activation of short- and medium-chain fatty acids and xenobiotic carboxylic acids." Vessey D.A., Kelley M., Warren R.S. Biochim. Biophys. Acta 1428:455-462(1999) [PubMed: 10434065] [Abstract] Cited for: SUBCELLULAR LOCATION, COFACTOR, TISSUE SPECIFICITY, ENZYME REGULATION. |
| [4] | "Comparative analyses of disease risk genes belonging to the acyl-CoA synthetase medium-chain (ACSM) family in human liver and cell lines." Boomgaarden I., Vock C., Klapper M., Doering F. Biochem. Genet. 47:739-748(2009) [PubMed: 19634011] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AY160217 mRNA. Translation: AAO17576.1. AB073604 mRNA. Translation: BAD38642.1. |
| IPI | IPI00329444. |
| RefSeq | NP_872423.3. |
| UniGene | Hs.298252 Hs.567879 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LCI based on UniProtKB P08659. |
| SMR | Q68CK6. Positions 33-569. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q68CK6. |
PTM databases | |
| PhosphoSite | Q68CK6. |
Proteomic databases | |
| PRIDE | Q68CK6. |
Genome annotation databases | |
| Ensembl | ENST00000329697; ENSP00000327453; ENSG00000066813; Homo sapiens. [Genome view] ENST00000414188; ENSP00000390378; ENSG00000066813; Homo sapiens. [Genome view] |
| GeneID | 348158. |
| UCSC | uc002dhj.2. human. |
Organism-specific databases | |
| CTD | 348158. |
| GeneCards | GC16M020457. |
| HGNC | HGNC:30931. ACSM2B. |
| PharmGKB | PA142672650. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG10263. |
| HOGENOM | HBG547964. |
| HOVERGEN | Q68CK6. |
| InParanoid | Q68CK6. |
Enzyme and pathway databases | |
| BRENDA | 6.2.1.2. 247. |
| Reactome | REACT_13433. Biological oxidations. |
Gene expression databases | |
| ArrayExpress | Q68CK6. |
| Bgee | Q68CK6. |
| CleanEx | HS_ACSM2B. |
| Genevestigator | Q68CK6. |
Family and domain databases | |
| InterPro | IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view] |
| Pfam | PF00501. AMP-binding. 1 hit. [Graphical view] |
| PROSITE | PS00455. AMP_BINDING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 99351. |
Entry information
| Entry name | ACS2B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q68CK6 Secondary accession number(s): Q86YT1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| SIMILARITY comments Index of protein domains and families |

Clusters with


