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Protein

Acyl-coenzyme A synthetase ACSM2B, mitochondrial

Gene

ACSM2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).1 Publication

Catalytic activityi

ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Enzyme regulationi

Activated by monovalent cations, such as potassium, rubidium or ammonium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei139Coenzyme ABy similarity1
Binding sitei364SubstrateBy similarity1
Binding sitei446ATPBy similarity1
Binding sitei461ATPBy similarity1
Binding sitei472SubstrateBy similarity1
Binding sitei501Coenzyme ABy similarity1
Binding sitei532Coenzyme ABy similarity1
Binding sitei557ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 229ATPBy similarity9
Nucleotide bindingi359 – 364ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00888-MONOMER.
ReactomeiR-HSA-177128. Conjugation of salicylate with glycine.
R-HSA-177135. Conjugation of benzoate with glycine.
R-HSA-177162. Conjugation of phenylacetate with glutamine.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A synthetase ACSM2B, mitochondrial (EC:6.2.1.2)
Alternative name(s):
Acyl-CoA synthetase medium-chain family member 2B
Butyrate--CoA ligase 2B
Butyryl-coenzyme A synthetase 2B
Middle-chain acyl-CoA synthetase 2B
Xenobiotic/medium-chain fatty acid-CoA ligase HXM-A
Gene namesi
Name:ACSM2B
Synonyms:ACSM2
ORF Names:HYST1046
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:30931. ACSM2B.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: GO_Central
  • mitochondrion Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi348158.
OpenTargetsiENSG00000066813.
PharmGKBiPA162375437.

Polymorphism and mutation databases

BioMutaiACSM2B.
DMDMi296434397.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46MitochondrionSequence analysisAdd BLAST46
ChainiPRO_000030609447 – 577Acyl-coenzyme A synthetase ACSM2B, mitochondrialAdd BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei141N6-acetyllysine; alternateBy similarity1
Modified residuei141N6-succinyllysine; alternateBy similarity1
Modified residuei327N6-acetyllysineBy similarity1
Modified residuei513PhosphoserineCombined sources1
Modified residuei525N6-acetyllysineBy similarity1
Modified residuei532N6-acetyllysine; alternateBy similarity1
Modified residuei532N6-succinyllysine; alternateBy similarity1
Modified residuei543N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ68CK6.
PaxDbiQ68CK6.
PeptideAtlasiQ68CK6.
PRIDEiQ68CK6.

PTM databases

iPTMnetiQ68CK6.
PhosphoSitePlusiQ68CK6.

Expressioni

Tissue specificityi

Detected in liver.2 Publications

Gene expression databases

BgeeiENSG00000066813.
CleanExiHS_ACSM2B.
ExpressionAtlasiQ68CK6. baseline and differential.
GenevisibleiQ68CK6. HS.

Organism-specific databases

HPAiHPA057699.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiQ68CK6. 2 interactors.
STRINGi9606.ENSP00000327453.

Structurei

3D structure databases

ProteinModelPortaliQ68CK6.
SMRiQ68CK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni469 – 471Coenzyme A bindingBy similarity3
Regioni540 – 542Coenzyme A bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiQ68CK6.
KOiK01896.
OMAiGMIVKAY.
OrthoDBiEOG091G03NW.
PhylomeDBiQ68CK6.
TreeFamiTF354264.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q68CK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWLRKVQGL CTLWGTQMSS RTLYINSRQL VSLQWGHQEV PAKFNFASDV
60 70 80 90 100
LDHWADMEKA GKRLPSPALW WVNGKGKELM WNFRELSENS QQAANILSGA
110 120 130 140 150
CGLQRGDRVA VMLPRVPEWW LVILGCIRAG LIFMPGTIQM KSTDILYRLQ
160 170 180 190 200
MSKAKAIVAG DEVIQEVDTV ASECPSLRIK LLVSEKSCDG WLNFKKLLNE
210 220 230 240 250
ASTTHHCVET GSQEASAIYF TSGTSGLPKM AEHSYSSLGL KAKMDAGWTG
260 270 280 290 300
LQASDIMWTI SDTGWILNIL GSLLESWTLG ACTFVHLLPK FDPLVILKTL
310 320 330 340 350
SSYPIKSMMG APIVYRMLLQ QDLSSYKFPH LQNCLAGGES LLPETLENWR
360 370 380 390 400
AQTGLDIREF YGQTETGLTC MVSKTMKIKP GYMGTAASCY DVQVIDDKGN
410 420 430 440 450
VLPPGTEGDI GIRVKPIRPI GIFSGYVENP DKTAANIRGD FWLLGDRGIK
460 470 480 490 500
DEDGYFQFMG RADDIINSSG YRIGPSEVEN ALMKHPAVVE TAVISSPDPV
510 520 530 540 550
RGEVVKAFVI LASQFLSHDP EQLTKELQQH VKSVTAPYKY PRKIEFVLNL
560 570
PKTVTGKIQR TKLRDKEWKM SGKARAQ
Length:577
Mass (Da):64,271
Last modified:May 18, 2010 - v2
Checksum:i93A6F0581A47F222
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46F → L in AAO17576 (PubMed:12616642).Curated1
Sequence conflicti96I → V in AAO17576 (PubMed:12616642).Curated1
Sequence conflicti96I → V in BAD38642 (PubMed:15221005).Curated1
Sequence conflicti133F → I in AAO17576 (PubMed:12616642).Curated1
Sequence conflicti136G → E in AAO17576 (PubMed:12616642).Curated1
Sequence conflicti143T → A in AAO17576 (PubMed:12616642).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY160217 mRNA. Translation: AAO17576.1.
AB073604 mRNA. Translation: BAD38642.1.
AC141053 Genomic DNA. No translation available.
AC141273 Genomic DNA. No translation available.
CCDSiCCDS10586.1.
RefSeqiNP_001098539.1. NM_001105069.1.
NP_872423.3. NM_182617.3.
UniGeneiHs.298252.
Hs.567879.

Genome annotation databases

EnsembliENST00000329697; ENSP00000327453; ENSG00000066813.
ENST00000414188; ENSP00000390378; ENSG00000066813.
ENST00000565232; ENSP00000454995; ENSG00000066813.
ENST00000567001; ENSP00000456378; ENSG00000066813.
GeneIDi348158.
KEGGihsa:348158.
UCSCiuc002dhj.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY160217 mRNA. Translation: AAO17576.1.
AB073604 mRNA. Translation: BAD38642.1.
AC141053 Genomic DNA. No translation available.
AC141273 Genomic DNA. No translation available.
CCDSiCCDS10586.1.
RefSeqiNP_001098539.1. NM_001105069.1.
NP_872423.3. NM_182617.3.
UniGeneiHs.298252.
Hs.567879.

3D structure databases

ProteinModelPortaliQ68CK6.
SMRiQ68CK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ68CK6. 2 interactors.
STRINGi9606.ENSP00000327453.

PTM databases

iPTMnetiQ68CK6.
PhosphoSitePlusiQ68CK6.

Polymorphism and mutation databases

BioMutaiACSM2B.
DMDMi296434397.

Proteomic databases

MaxQBiQ68CK6.
PaxDbiQ68CK6.
PeptideAtlasiQ68CK6.
PRIDEiQ68CK6.

Protocols and materials databases

DNASUi348158.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329697; ENSP00000327453; ENSG00000066813.
ENST00000414188; ENSP00000390378; ENSG00000066813.
ENST00000565232; ENSP00000454995; ENSG00000066813.
ENST00000567001; ENSP00000456378; ENSG00000066813.
GeneIDi348158.
KEGGihsa:348158.
UCSCiuc002dhj.5. human.

Organism-specific databases

CTDi348158.
DisGeNETi348158.
GeneCardsiACSM2B.
H-InvDBHIX0026968.
HGNCiHGNC:30931. ACSM2B.
HPAiHPA057699.
MIMi614359. gene.
neXtProtiNX_Q68CK6.
OpenTargetsiENSG00000066813.
PharmGKBiPA162375437.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00760000119178.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiQ68CK6.
KOiK01896.
OMAiGMIVKAY.
OrthoDBiEOG091G03NW.
PhylomeDBiQ68CK6.
TreeFamiTF354264.

Enzyme and pathway databases

BioCyciZFISH:HS00888-MONOMER.
ReactomeiR-HSA-177128. Conjugation of salicylate with glycine.
R-HSA-177135. Conjugation of benzoate with glycine.
R-HSA-177162. Conjugation of phenylacetate with glutamine.

Miscellaneous databases

ChiTaRSiACSM2B. human.
GeneWikiiACSM2B.
GenomeRNAii348158.
PROiQ68CK6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000066813.
CleanExiHS_ACSM2B.
ExpressionAtlasiQ68CK6. baseline and differential.
GenevisibleiQ68CK6. HS.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACS2B_HUMAN
AccessioniPrimary (citable) accession number: Q68CK6
Secondary accession number(s): Q86YT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.