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Q68CK6

- ACS2B_HUMAN

UniProt

Q68CK6 - ACS2B_HUMAN

Protein

Acyl-coenzyme A synthetase ACSM2B, mitochondrial

Gene

ACSM2B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
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    Functioni

    Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).1 Publication

    Catalytic activityi

    ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium or manganese.1 Publication

    Enzyme regulationi

    Activated by monovalent cations, such as potassium, rubidium or ammonium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei139 – 1391Coenzyme ABy similarity
    Binding sitei364 – 3641SubstrateBy similarity
    Binding sitei446 – 4461ATPBy similarity
    Binding sitei461 – 4611ATPBy similarity
    Binding sitei472 – 4721SubstrateBy similarity
    Binding sitei501 – 5011Coenzyme ABy similarity
    Binding sitei532 – 5321Coenzyme ABy similarity
    Binding sitei557 – 5571ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi221 – 2299ATPBy similarity
    Nucleotide bindingi359 – 3646ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. butyrate-CoA ligase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB-KW
    2. small molecule metabolic process Source: Reactome
    3. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_6800. Conjugation of phenylacetate with glutamine.
    REACT_6812. Conjugation of salicylate with glycine.
    REACT_6933. Conjugation of benzoate with glycine.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A synthetase ACSM2B, mitochondrial (EC:6.2.1.2)
    Alternative name(s):
    Acyl-CoA synthetase medium-chain family member 2B
    Butyrate--CoA ligase 2B
    Butyryl-coenzyme A synthetase 2B
    Middle-chain acyl-CoA synthetase 2B
    Xenobiotic/medium-chain fatty acid-CoA ligase HXM-A
    Gene namesi
    Name:ACSM2B
    Synonyms:ACSM2
    ORF Names:HYST1046
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:30931. ACSM2B.

    Subcellular locationi

    Mitochondrion matrix 3 Publications

    GO - Cellular componenti

    1. mitochondrial matrix Source: Reactome
    2. mitochondrion Source: BHF-UCL

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162375437.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4646MitochondrionSequence AnalysisAdd
    BLAST
    Chaini47 – 577531Acyl-coenzyme A synthetase ACSM2B, mitochondrialPRO_0000306094Add
    BLAST

    Proteomic databases

    MaxQBiQ68CK6.
    PaxDbiQ68CK6.
    PRIDEiQ68CK6.

    PTM databases

    PhosphoSiteiQ68CK6.

    Expressioni

    Tissue specificityi

    Detected in liver.2 Publications

    Gene expression databases

    ArrayExpressiQ68CK6.
    BgeeiQ68CK6.
    CleanExiHS_ACSM2B.
    GenevestigatoriQ68CK6.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    IntActiQ68CK6. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ68CK6.
    SMRiQ68CK6. Positions 34-569.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni469 – 4713Coenzyme A bindingBy similarity
    Regioni540 – 5423Coenzyme A bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229982.
    HOVERGENiHBG053031.
    InParanoidiQ68CK6.
    KOiK01896.
    OMAiTICYTAP.
    OrthoDBiEOG7D85VZ.
    PhylomeDBiQ68CK6.
    TreeFamiTF354264.

    Family and domain databases

    InterProiIPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q68CK6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHWLRKVQGL CTLWGTQMSS RTLYINSRQL VSLQWGHQEV PAKFNFASDV    50
    LDHWADMEKA GKRLPSPALW WVNGKGKELM WNFRELSENS QQAANILSGA 100
    CGLQRGDRVA VMLPRVPEWW LVILGCIRAG LIFMPGTIQM KSTDILYRLQ 150
    MSKAKAIVAG DEVIQEVDTV ASECPSLRIK LLVSEKSCDG WLNFKKLLNE 200
    ASTTHHCVET GSQEASAIYF TSGTSGLPKM AEHSYSSLGL KAKMDAGWTG 250
    LQASDIMWTI SDTGWILNIL GSLLESWTLG ACTFVHLLPK FDPLVILKTL 300
    SSYPIKSMMG APIVYRMLLQ QDLSSYKFPH LQNCLAGGES LLPETLENWR 350
    AQTGLDIREF YGQTETGLTC MVSKTMKIKP GYMGTAASCY DVQVIDDKGN 400
    VLPPGTEGDI GIRVKPIRPI GIFSGYVENP DKTAANIRGD FWLLGDRGIK 450
    DEDGYFQFMG RADDIINSSG YRIGPSEVEN ALMKHPAVVE TAVISSPDPV 500
    RGEVVKAFVI LASQFLSHDP EQLTKELQQH VKSVTAPYKY PRKIEFVLNL 550
    PKTVTGKIQR TKLRDKEWKM SGKARAQ 577
    Length:577
    Mass (Da):64,271
    Last modified:May 18, 2010 - v2
    Checksum:i93A6F0581A47F222
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461F → L in AAO17576. (PubMed:12616642)Curated
    Sequence conflicti96 – 961I → V in AAO17576. (PubMed:12616642)Curated
    Sequence conflicti96 – 961I → V in BAD38642. (PubMed:15221005)Curated
    Sequence conflicti133 – 1331F → I in AAO17576. (PubMed:12616642)Curated
    Sequence conflicti136 – 1361G → E in AAO17576. (PubMed:12616642)Curated
    Sequence conflicti143 – 1431T → A in AAO17576. (PubMed:12616642)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY160217 mRNA. Translation: AAO17576.1.
    AB073604 mRNA. Translation: BAD38642.1.
    AC141053 Genomic DNA. No translation available.
    AC141273 Genomic DNA. No translation available.
    CCDSiCCDS10586.1.
    RefSeqiNP_001098539.1. NM_001105069.1.
    NP_872423.3. NM_182617.3.
    UniGeneiHs.298252.
    Hs.567879.

    Genome annotation databases

    EnsembliENST00000329697; ENSP00000327453; ENSG00000066813.
    ENST00000565232; ENSP00000454995; ENSG00000066813.
    ENST00000567001; ENSP00000456378; ENSG00000066813.
    GeneIDi348158.
    KEGGihsa:348158.
    UCSCiuc002dhj.4. human.

    Polymorphism databases

    DMDMi296434397.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY160217 mRNA. Translation: AAO17576.1 .
    AB073604 mRNA. Translation: BAD38642.1 .
    AC141053 Genomic DNA. No translation available.
    AC141273 Genomic DNA. No translation available.
    CCDSi CCDS10586.1.
    RefSeqi NP_001098539.1. NM_001105069.1.
    NP_872423.3. NM_182617.3.
    UniGenei Hs.298252.
    Hs.567879.

    3D structure databases

    ProteinModelPortali Q68CK6.
    SMRi Q68CK6. Positions 34-569.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q68CK6. 1 interaction.

    PTM databases

    PhosphoSitei Q68CK6.

    Polymorphism databases

    DMDMi 296434397.

    Proteomic databases

    MaxQBi Q68CK6.
    PaxDbi Q68CK6.
    PRIDEi Q68CK6.

    Protocols and materials databases

    DNASUi 348158.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000329697 ; ENSP00000327453 ; ENSG00000066813 .
    ENST00000565232 ; ENSP00000454995 ; ENSG00000066813 .
    ENST00000567001 ; ENSP00000456378 ; ENSG00000066813 .
    GeneIDi 348158.
    KEGGi hsa:348158.
    UCSCi uc002dhj.4. human.

    Organism-specific databases

    CTDi 348158.
    GeneCardsi GC16M020547.
    H-InvDB HIX0026968.
    HGNCi HGNC:30931. ACSM2B.
    MIMi 614359. gene.
    neXtProti NX_Q68CK6.
    PharmGKBi PA162375437.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229982.
    HOVERGENi HBG053031.
    InParanoidi Q68CK6.
    KOi K01896.
    OMAi TICYTAP.
    OrthoDBi EOG7D85VZ.
    PhylomeDBi Q68CK6.
    TreeFami TF354264.

    Enzyme and pathway databases

    Reactomei REACT_6800. Conjugation of phenylacetate with glutamine.
    REACT_6812. Conjugation of salicylate with glycine.
    REACT_6933. Conjugation of benzoate with glycine.

    Miscellaneous databases

    GeneWikii ACSM2B.
    GenomeRNAii 348158.
    NextBioi 99351.
    PROi Q68CK6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q68CK6.
    Bgeei Q68CK6.
    CleanExi HS_ACSM2B.
    Genevestigatori Q68CK6.

    Family and domain databases

    InterProi IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequencing, and expression of a cDNA for the HXM-A form of xenobiotic/medium-chain fatty acid:CoA ligase from human liver mitochondria."
      Vessey D.A., Lau E., Kelley M., Warren R.S.
      J. Biochem. Mol. Toxicol. 17:1-6(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 152-163 AND 483-491, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
      Tissue: Liver.
    2. "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas."
      Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A.
      Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    3. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Characterization of the CoA ligases of human liver mitochondria catalyzing the activation of short- and medium-chain fatty acids and xenobiotic carboxylic acids."
      Vessey D.A., Kelley M., Warren R.S.
      Biochim. Biophys. Acta 1428:455-462(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, COFACTOR, TISSUE SPECIFICITY, ENZYME REGULATION.
    5. "Comparative analyses of disease risk genes belonging to the acyl-CoA synthetase medium-chain (ACSM) family in human liver and cell lines."
      Boomgaarden I., Vock C., Klapper M., Doering F.
      Biochem. Genet. 47:739-748(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiACS2B_HUMAN
    AccessioniPrimary (citable) accession number: Q68CK6
    Secondary accession number(s): Q86YT1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3