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Protein

Toll-like receptor 2

Gene

TLR2

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). May also promote apoptosis in response to lipoproteins. Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 2
Alternative name(s):
CD_antigen: CD282
Gene namesi
Name:TLR2
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

  • Membrane By similarity; Single-pass type I membrane protein Sequence analysis
  • Cytoplasmic vesiclephagosome membrane By similarity; Single-pass type I membrane protein Sequence analysis
  • Membrane raft By similarity

  • Note: Does not reside in lipid rafts before stimulation but accumulates increasingly in the raft upon the presence of the microbial ligand. In response to diacylated lipoproteins, TLR2:TLR6 heterodimers are recruited in lipid rafts, this recruitment determine the intracellular targeting to the Golgi apparatus. Triacylated lipoproteins induce the same mechanism for TLR2:TLR1 heterodimers.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 589ExtracellularSequence analysisAdd BLAST572
Transmembranei590 – 610HelicalSequence analysisAdd BLAST21
Topological domaini611 – 785CytoplasmicSequence analysisAdd BLAST175

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000003470818 – 785Toll-like receptor 2Add BLAST768

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 37By similarity
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi354 ↔ 383By similarity
Glycosylationi415N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi433 ↔ 455By similarity
Glycosylationi443N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Interacts with LY96, TLR1 and TLR6 (via extracellular domain). TLR2 seems to exist in heterodimers with either TLR1 or TLR6 before stimulation by the ligand. The heterodimers form bigger oligomers in response to their corresponding ligands as well as further heterotypic associations with other receptors such as CD14 and/or CD36. Binds MYD88 (via TIR domain). Interacts with TICAM1 (By similarity). Interacts with CNPY3 (By similarity). Interacts with ATG16L1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei350Interaction with bacterial lipopeptideBy similarity1

Structurei

3D structure databases

ProteinModelPortaliQ689D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati54 – 77LRR 1Add BLAST24
Repeati78 – 101LRR 2Add BLAST24
Repeati102 – 125LRR 3Add BLAST24
Repeati126 – 150LRR 4Add BLAST25
Repeati151 – 175LRR 5Add BLAST25
Repeati176 – 199LRR 6Add BLAST24
Repeati200 – 223LRR 7Add BLAST24
Repeati224 – 250LRR 8Add BLAST27
Repeati251 – 278LRR 9Add BLAST28
Repeati279 – 308LRR 10Add BLAST30
Repeati309 – 337LRR 11Add BLAST29
Repeati338 – 361LRR 12Add BLAST24
Repeati362 – 388LRR 13Add BLAST27
Repeati389 – 414LRR 14Add BLAST26
Repeati415 – 437LRR 15Add BLAST23
Repeati438 – 457LRR 16Add BLAST20
Repeati458 – 478LRR 17Add BLAST21
Repeati479 – 500LRR 18Add BLAST22
Repeati501 – 524LRR 19Add BLAST24
Domaini525 – 579LRRCTAdd BLAST55
Domaini640 – 785TIRPROSITE-ProRule annotationAdd BLAST146

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi762 – 779ATG16L1-binding motifAdd BLAST18

Domaini

Ester-bound lipid substrates are bound through a crevice formed between the LRR 11 and LRR 12.By similarity
The ATG16L1-binding motif mediates interaction with ATG16L1.By similarity

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 19 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000110611.
HOVERGENiHBG108574.
InParanoidiQ689D1.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027185. TLR2.
IPR017241. Toll-like_receptor.
[Graphical view]
PANTHERiPTHR24365:SF17. PTHR24365:SF17. 1 hit.
PfamiPF13855. LRR_8. 2 hits.
PF01463. LRRCT. 1 hit.
PF01582. TIR. 1 hit.
[Graphical view]
PIRSFiPIRSF037595. Toll-like_receptor. 1 hit.
SMARTiSM00369. LRR_TYP. 7 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q689D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVLWTLWV LGAVTNLSKE EAPDQSSSLS CDPTGVCDGR SRSLNSMPSG
60 70 80 90 100
LTAAVRSLDL SNNEITYIGN SDLRDCVNLK ALRLESNGIN TIEEESFLSL
110 120 130 140 150
WSLEHLDLSY NLLSNLSSSW FRPLSSLKFL NLLGNPYKSL GETPLFSQLT
160 170 180 190 200
NLRILKVGNI YSFTEIQDKD FAGLTFLEEL EIDASNLQRY EPKSLKSIQN
210 220 230 240 250
ISYLALRMKQ PVLLVEIFVD LSSSLKHLEL RDTHLDTFHF SEASINETHT
260 270 280 290 300
LVKKWTFRNV KVTDRSFTGV VRLLNYVSGV LEVEFEDCTL YGLGDFDIPD
310 320 330 340 350
VDKIKNIGQI ETLTVRRLHI PHFYSFYDMS SIYSLTEDVK RITVENSKVF
360 370 380 390 400
LVPCLLSQHL KSLEYLDLSE NLMVEEYLKN SACEDAWPSL QTLVLRQNHL
410 420 430 440 450
ASLERTGETL LTLKNLTNID ISKNSFHSMP ETCQWPEKMK YLNLSSTRIH
460 470 480 490 500
SVTGCIPKTL EILDVSNNNL NLFSLNLPQL KELYISRNKL MTLPDASLLP
510 520 530 540 550
MLLVLKISRN AITTFSKEQL DSFHTLKTLE AGGNNFICSC EFLSFTQEQQ
560 570 580 590 600
ALAKVLIDWP ANYLCDSPSH VRGQQVQDVR LSVSECHRTA LVSGMCCALF
610 620 630 640 650
LLILLTEVLC HRFHGLWYMR MMWAWLQAKR KPRKAPSRDV CYDAFVSYSE
660 670 680 690 700
HDSYWVENLL VQKLEHFNPP FKLCLHKRDF IPGKWIIDNI IDSIEKSRKT
710 720 730 740 750
IFVLSENFVK SEWCKYELDF SHFRLFDENS DAAILILLEP IEKKAIPQRF
760 770 780
CKLRKIMNTK TYLEWPTDDA QQEGFWLNLR TAIKS
Length:785
Mass (Da):90,277
Last modified:October 11, 2004 - v1
Checksum:i717606848DD91881
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB189639 mRNA. Translation: BAD42423.1.
UniGeneiCfa.15802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB189639 mRNA. Translation: BAD42423.1.
UniGeneiCfa.15802.

3D structure databases

ProteinModelPortaliQ689D1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000110611.
HOVERGENiHBG108574.
InParanoidiQ689D1.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027185. TLR2.
IPR017241. Toll-like_receptor.
[Graphical view]
PANTHERiPTHR24365:SF17. PTHR24365:SF17. 1 hit.
PfamiPF13855. LRR_8. 2 hits.
PF01463. LRRCT. 1 hit.
PF01582. TIR. 1 hit.
[Graphical view]
PIRSFiPIRSF037595. Toll-like_receptor. 1 hit.
SMARTiSM00369. LRR_TYP. 7 hits.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLR2_CANLF
AccessioniPrimary (citable) accession number: Q689D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 11, 2004
Last modified: October 5, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.