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Protein

Toll-like receptor 2

Gene

TLR2

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei350 – 3501Interaction with bacterial lipopeptideBy similarity

GO - Molecular functioni

  1. transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

  1. inflammatory response Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
  3. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
  4. positive regulation of inflammatory response Source: InterPro
  5. regulation of cytokine secretion Source: InterPro
  6. response to molecule of bacterial origin Source: InterPro
  7. toll-like receptor 2 signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 2
Alternative name(s):
CD_antigen: CD282
Gene namesi
Name:TLR2
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Unplaced

Subcellular locationi

Membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 589572ExtracellularSequence AnalysisAdd
BLAST
Transmembranei590 – 61021HelicalSequence AnalysisAdd
BLAST
Topological domaini611 – 785175CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 785768Toll-like receptor 2PRO_0000034708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 37By similarity
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi354 ↔ 383By similarity
Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi433 ↔ 455By similarity
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ689D1.

Interactioni

Subunit structurei

Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1 or TLR6. Interacts with CNPY3. Interacts with ATG16L1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ689D1.
SMRiQ689D1. Positions 28-554, 640-785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati54 – 7724LRR 1Add
BLAST
Repeati78 – 10124LRR 2Add
BLAST
Repeati102 – 12524LRR 3Add
BLAST
Repeati126 – 15025LRR 4Add
BLAST
Repeati151 – 17525LRR 5Add
BLAST
Repeati176 – 19924LRR 6Add
BLAST
Repeati200 – 22324LRR 7Add
BLAST
Repeati224 – 25027LRR 8Add
BLAST
Repeati251 – 27828LRR 9Add
BLAST
Repeati279 – 30830LRR 10Add
BLAST
Repeati309 – 33729LRR 11Add
BLAST
Repeati338 – 36124LRR 12Add
BLAST
Repeati362 – 38827LRR 13Add
BLAST
Repeati389 – 41426LRR 14Add
BLAST
Repeati415 – 43723LRR 15Add
BLAST
Repeati438 – 45720LRR 16Add
BLAST
Repeati458 – 47821LRR 17Add
BLAST
Repeati479 – 50022LRR 18Add
BLAST
Repeati501 – 52424LRR 19Add
BLAST
Domaini525 – 57955LRRCTAdd
BLAST
Domaini640 – 785146TIRPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi762 – 77918ATG16L1-binding motifAdd
BLAST

Domaini

Ester-bound lipid substrates are bound through a crevice formed between the LRR 11 and LRR 12.By similarity
The ATG16L1-binding motif mediates interaction with ATG16L1.By similarity

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 19 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG251801.
HOGENOMiHOG000110611.
HOVERGENiHBG108574.
InParanoidiQ689D1.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027185. TLR2.
IPR017241. Toll-like_receptor.
[Graphical view]
PANTHERiPTHR24365:SF17. PTHR24365:SF17. 1 hit.
PfamiPF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
PIRSFiPIRSF037595. Toll-like_receptor. 1 hit.
SMARTiSM00369. LRR_TYP. 1 hit.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q689D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVLWTLWV LGAVTNLSKE EAPDQSSSLS CDPTGVCDGR SRSLNSMPSG
60 70 80 90 100
LTAAVRSLDL SNNEITYIGN SDLRDCVNLK ALRLESNGIN TIEEESFLSL
110 120 130 140 150
WSLEHLDLSY NLLSNLSSSW FRPLSSLKFL NLLGNPYKSL GETPLFSQLT
160 170 180 190 200
NLRILKVGNI YSFTEIQDKD FAGLTFLEEL EIDASNLQRY EPKSLKSIQN
210 220 230 240 250
ISYLALRMKQ PVLLVEIFVD LSSSLKHLEL RDTHLDTFHF SEASINETHT
260 270 280 290 300
LVKKWTFRNV KVTDRSFTGV VRLLNYVSGV LEVEFEDCTL YGLGDFDIPD
310 320 330 340 350
VDKIKNIGQI ETLTVRRLHI PHFYSFYDMS SIYSLTEDVK RITVENSKVF
360 370 380 390 400
LVPCLLSQHL KSLEYLDLSE NLMVEEYLKN SACEDAWPSL QTLVLRQNHL
410 420 430 440 450
ASLERTGETL LTLKNLTNID ISKNSFHSMP ETCQWPEKMK YLNLSSTRIH
460 470 480 490 500
SVTGCIPKTL EILDVSNNNL NLFSLNLPQL KELYISRNKL MTLPDASLLP
510 520 530 540 550
MLLVLKISRN AITTFSKEQL DSFHTLKTLE AGGNNFICSC EFLSFTQEQQ
560 570 580 590 600
ALAKVLIDWP ANYLCDSPSH VRGQQVQDVR LSVSECHRTA LVSGMCCALF
610 620 630 640 650
LLILLTEVLC HRFHGLWYMR MMWAWLQAKR KPRKAPSRDV CYDAFVSYSE
660 670 680 690 700
HDSYWVENLL VQKLEHFNPP FKLCLHKRDF IPGKWIIDNI IDSIEKSRKT
710 720 730 740 750
IFVLSENFVK SEWCKYELDF SHFRLFDENS DAAILILLEP IEKKAIPQRF
760 770 780
CKLRKIMNTK TYLEWPTDDA QQEGFWLNLR TAIKS
Length:785
Mass (Da):90,277
Last modified:October 10, 2004 - v1
Checksum:i717606848DD91881
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB189639 mRNA. Translation: BAD42423.1.
UniGeneiCfa.15802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB189639 mRNA. Translation: BAD42423.1.
UniGeneiCfa.15802.

3D structure databases

ProteinModelPortaliQ689D1.
SMRiQ689D1. Positions 28-554, 640-785.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ689D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG251801.
HOGENOMiHOG000110611.
HOVERGENiHBG108574.
InParanoidiQ689D1.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000157. TIR_dom.
IPR027185. TLR2.
IPR017241. Toll-like_receptor.
[Graphical view]
PANTHERiPTHR24365:SF17. PTHR24365:SF17. 1 hit.
PfamiPF13855. LRR_8. 3 hits.
PF01582. TIR. 1 hit.
[Graphical view]
PIRSFiPIRSF037595. Toll-like_receptor. 1 hit.
SMARTiSM00369. LRR_TYP. 1 hit.
SM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 11 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of canine Toll-like receptor 2 (TLR2) gene."
    Ishii M., Kano R., Hasegawa A.
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTLR2_CANFA
AccessioniPrimary (citable) accession number: Q689D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 25, 2005
Last sequence update: October 10, 2004
Last modified: January 6, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.