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Protein

Rap1 GTPase-activating protein 2

Gene

RAP1GAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_23898. Rap1 signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Rap1 GTPase-activating protein 2
Short name:
Rap1GAP2
Alternative name(s):
GTPase-activating Rap/Ran-GAP domain-like protein 4
Gene namesi
Name:RAP1GAP2
Synonyms:GARNL4, KIAA1039, RAP1GA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:29176. RAP1GAP2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cytoplasmperinuclear region 1 Publication

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • neuron projection Source: Ensembl
  • nuclear membrane Source: HPA
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71S → A: Abolishes phosphorylation by PKG/PRKG1. 1 Publication
Mutagenesisi357 – 3571N → A: Abolishes GAP activity. 1 Publication

Organism-specific databases

PharmGKBiPA165432528.

Polymorphism and mutation databases

BioMutaiRAP1GAP2.
DMDMi162416269.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Rap1 GTPase-activating protein 2PRO_0000312716Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71Phosphoserine; by PKG/PRKG1; in vitro1 Publication
Modified residuei45 – 451Phosphoserine2 Publications
Modified residuei49 – 491Phosphothreonine2 Publications
Modified residuei544 – 5441Phosphoserine2 Publications
Modified residuei558 – 5581Phosphoserine1 Publication
Modified residuei564 – 5641Phosphoserine1 Publication

Post-translational modificationi

In vitro phosphorylated by cGMP-dependent protein kinase 1 (cGKI) at Ser-7; the phosphorylation probably does not regulate GAP activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ684P5.
PaxDbiQ684P5.
PRIDEiQ684P5.

PTM databases

PhosphoSiteiQ684P5.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in platelets with isoform 2 being the predominant form. Expressed in lymphocytes, heart, testis and pancreas.1 Publication

Gene expression databases

BgeeiQ684P5.
CleanExiHS_GARNL4.
GenevisibleiQ684P5. HS.

Organism-specific databases

HPAiHPA022148.
HPA022896.

Interactioni

Protein-protein interaction databases

BioGridi116732. 3 interactions.
IntActiQ684P5. 2 interactions.
STRINGi9606.ENSP00000254695.

Structurei

3D structure databases

ProteinModelPortaliQ684P5.
SMRiQ684P5. Positions 147-479.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini248 – 464217Rap-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi586 – 726141Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 Rap-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG292109.
GeneTreeiENSGT00760000119182.
HOGENOMiHOG000231640.
HOVERGENiHBG016371.
InParanoidiQ684P5.
KOiK17708.
OMAiHNSVEVT.
OrthoDBiEOG7WHH8V.
PhylomeDBiQ684P5.
TreeFamiTF318626.

Family and domain databases

InterProiIPR000331. Rap_GAP_dom.
[Graphical view]
PfamiPF02145. Rap_GAP. 1 hit.
[Graphical view]
PROSITEiPS50085. RAPGAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q684P5-1) [UniParc]FASTAAdd to basket

Also known as: Rap1GAP2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGRKRSVSF GGFGWIDKTM LASLKVKKQE LANSSDATLP DRPLSPPLTA
60 70 80 90 100
PPTMKSSEFF EMLEKMQGIK LEEQKPGPQK NKDDYIPYPS IDEVVEKGGP
110 120 130 140 150
YPQVILPQFG GYWIEDPENV GTPTSLGSSI CEEEEEDNLS PNTFGYKLEC
160 170 180 190 200
KGEARAYRRH FLGKDHLNFY CTGSSLGNLI LSVKCEEAEG IEYLRVILRS
210 220 230 240 250
KLKTVHERIP LAGLSKLPSV PQIAKAFCDD AVGLRFNPVL YPKASQMIVS
260 270 280 290 300
YDEHEVNNTF KFGVIYQKAR QTLEEELFGN NEESPAFKEF LDLLGDTITL
310 320 330 340 350
QDFKGFRGGL DVTHGQTGVE SVYTTFRDRE IMFHVSTKLP FTDGDAQQLQ
360 370 380 390 400
RKRHIGNDIV AIIFQEENTP FVPDMIASNF LHAYIVVQVE TPGTETPSYK
410 420 430 440 450
VSVTAREDVP TFGPPLPSPP VFQKGPEFRE FLLTKLTNAE NACCKSDKFA
460 470 480 490 500
KLEDRTRAAL LDNLHDELHA HTQAMLGLGP EEDKFENGGH GGFLESFKRA
510 520 530 540 550
IRVRSHSMET MVGGQKKSHS GGIPGSLSGG ISHNSMEVTK TTFSPPVVAA
560 570 580 590 600
TVKNQSRSPI KRRSGLFPRL HTGSEGQGDS RARCDSTSST PKTPDGGHSS
610 620 630 640 650
QEIKSETSSN PSSPEICPNK EKPFMKLKEN GRAISRSSSS TSSVSSTAGE
660 670 680 690 700
GEAMEEGDSG GSQPSTTSPF KQEVFVYSPS PSSESPSLGA AATPIIMSRS
710 720 730
PTDAKSRNSP RSNLKFRFDK LSHASSGAGH
Length:730
Mass (Da):80,056
Last modified:December 4, 2007 - v2
Checksum:i8E7584ADC2DA0B08
GO
Isoform 2 (identifier: Q684P5-2) [UniParc]FASTAAdd to basket

Also known as: Rap1GAP2a

The sequence of this isoform differs from the canonical sequence as follows:
     68-82: Missing.

Show »
Length:715
Mass (Da):78,380
Checksum:i3A8175583D2C9C3F
GO
Isoform 3 (identifier: Q684P5-3) [UniParc]FASTAAdd to basket

Also known as: Rap1GAP2c

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Show »
Length:711
Mass (Da):77,897
Checksum:iF90B7B7CBC1F46A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti285 – 2851P → L in BAC85912 (PubMed:14702039).Curated
Sequence conflicti304 – 3041K → N in CAF31652 (PubMed:15632203).Curated
Sequence conflicti304 – 3041K → N in CAF31653 (PubMed:15632203).Curated
Sequence conflicti726 – 7261S → P in CAH10514 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti202 – 2021L → M.
Corresponds to variant rs17762452 [ dbSNP | Ensembl ].
VAR_037553

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919Missing in isoform 3. 2 PublicationsVSP_029888Add
BLAST
Alternative sequencei68 – 8215Missing in isoform 2. 1 PublicationVSP_029889Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ628446 mRNA. Translation: CAF31652.1.
AJ628447 mRNA. Translation: CAF31653.1.
AK124640 mRNA. Translation: BAC85912.1.
CR627427 mRNA. Translation: CAH10514.1.
BC140870 mRNA. Translation: AAI40871.1.
BC140871 mRNA. Translation: AAI40872.1.
AB028962 mRNA. Translation: BAA82991.1.
CCDSiCCDS45573.1. [Q684P5-1]
CCDS45574.1. [Q684P5-2]
RefSeqiNP_001093868.1. NM_001100398.1. [Q684P5-2]
NP_055900.4. NM_015085.4. [Q684P5-1]
XP_006721540.1. XM_006721477.2. [Q684P5-3]
UniGeneiHs.499659.

Genome annotation databases

EnsembliENST00000254695; ENSP00000254695; ENSG00000132359. [Q684P5-1]
ENST00000366401; ENSP00000389824; ENSG00000132359. [Q684P5-2]
ENST00000540393; ENSP00000439688; ENSG00000132359. [Q684P5-3]
ENST00000542807; ENSP00000444890; ENSG00000132359. [Q684P5-1]
GeneIDi23108.
KEGGihsa:23108.
UCSCiuc010ckd.3. human. [Q684P5-1]
uc010cke.3. human. [Q684P5-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ628446 mRNA. Translation: CAF31652.1.
AJ628447 mRNA. Translation: CAF31653.1.
AK124640 mRNA. Translation: BAC85912.1.
CR627427 mRNA. Translation: CAH10514.1.
BC140870 mRNA. Translation: AAI40871.1.
BC140871 mRNA. Translation: AAI40872.1.
AB028962 mRNA. Translation: BAA82991.1.
CCDSiCCDS45573.1. [Q684P5-1]
CCDS45574.1. [Q684P5-2]
RefSeqiNP_001093868.1. NM_001100398.1. [Q684P5-2]
NP_055900.4. NM_015085.4. [Q684P5-1]
XP_006721540.1. XM_006721477.2. [Q684P5-3]
UniGeneiHs.499659.

3D structure databases

ProteinModelPortaliQ684P5.
SMRiQ684P5. Positions 147-479.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116732. 3 interactions.
IntActiQ684P5. 2 interactions.
STRINGi9606.ENSP00000254695.

PTM databases

PhosphoSiteiQ684P5.

Polymorphism and mutation databases

BioMutaiRAP1GAP2.
DMDMi162416269.

Proteomic databases

MaxQBiQ684P5.
PaxDbiQ684P5.
PRIDEiQ684P5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254695; ENSP00000254695; ENSG00000132359. [Q684P5-1]
ENST00000366401; ENSP00000389824; ENSG00000132359. [Q684P5-2]
ENST00000540393; ENSP00000439688; ENSG00000132359. [Q684P5-3]
ENST00000542807; ENSP00000444890; ENSG00000132359. [Q684P5-1]
GeneIDi23108.
KEGGihsa:23108.
UCSCiuc010ckd.3. human. [Q684P5-1]
uc010cke.3. human. [Q684P5-2]

Organism-specific databases

CTDi23108.
GeneCardsiGC17P002680.
H-InvDBHIX0013424.
HIX0039496.
HGNCiHGNC:29176. RAP1GAP2.
HPAiHPA022148.
HPA022896.
neXtProtiNX_Q684P5.
PharmGKBiPA165432528.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG292109.
GeneTreeiENSGT00760000119182.
HOGENOMiHOG000231640.
HOVERGENiHBG016371.
InParanoidiQ684P5.
KOiK17708.
OMAiHNSVEVT.
OrthoDBiEOG7WHH8V.
PhylomeDBiQ684P5.
TreeFamiTF318626.

Enzyme and pathway databases

ReactomeiREACT_23898. Rap1 signalling.

Miscellaneous databases

ChiTaRSiRAP1GAP2. human.
GeneWikiiRAP1GAP2.
GenomeRNAii23108.
NextBioi44297.
PROiQ684P5.

Gene expression databases

BgeeiQ684P5.
CleanExiHS_GARNL4.
GenevisibleiQ684P5. HS.

Family and domain databases

InterProiIPR000331. Rap_GAP_dom.
[Graphical view]
PfamiPF02145. Rap_GAP. 1 hit.
[Graphical view]
PROSITEiPS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rap1GAP2 is a new GTPase-activating protein of Rap1 expressed in human platelets."
    Schultess J., Danielewski O., Smolenski A.P.
    Blood 105:3185-3192(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION AT SER-7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF SER-7 AND ASN-357.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Cervix.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-730.
    Tissue: Brain.
  6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
    Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
    Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558 AND SER-564, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRPGP2_HUMAN
AccessioniPrimary (citable) accession number: Q684P5
Secondary accession number(s): B2RTY5
, Q684P4, Q6AI00, Q6ZVF0, Q9UPW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: June 24, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.