Q684P5 (RPGP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rap1 GTPase-activating protein 2
Short name=Rap1GAP2

Alternative name(s):
GTPase-activating Rap/Ran-GAP domain-like protein 4

Gene names

Name:	RAP1GAP2
Synonyms:	GARNL4, KIAA1039, RAP1GA2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state. Ref.1
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region Ref.1.
Tissue specificity	Isoform 1 and isoform 2 are expressed in platelets with isoform 2 being the predominant form. Expressed in lymphocytes, heart, testis and pancreas. Ref.1
Post-translational modification	In vitro phosphorylated by cGMP-dependent protein kinase 1 (cGKI) at Ser-7; the phosphorylation probably does not regulate GAP activity. Ref.1
Sequence similarities	Contains 1 Rap-GAP domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	centrosome Inferred from direct assay PubMed 21399614. Source: UniProtKB cytosol Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q684P5-1)

Also known as: Rap1GAP2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q684P5-2)

Also known as: Rap1GAP2a;

The sequence of this isoform differs from the canonical sequence as follows:
     68-82: Missing.

Isoform 3 (identifier: Q684P5-3)

Also known as: Rap1GAP2c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Rap1 GTPase-activating protein 2		PRO_0000312716
Regions
	Domain	248 – 464	217	Rap-GAP
	Compositional bias	586 – 726	141	Ser-rich
Amino acid modifications
	Modified residue	7	1	Phosphoserine; by PKG/PRKG1; in vitro Ref.1
	Modified residue	45	1	Phosphoserine Ref.7 Ref.8
	Modified residue	49	1	Phosphothreonine Ref.7 Ref.8
	Modified residue	544	1	Phosphoserine Ref.6 Ref.8
	Modified residue	558	1	Phosphoserine Ref.9
	Modified residue	564	1	Phosphoserine Ref.9
	Modified residue	613	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 19	19	Missing in isoform 3.		VSP_029888
	Alternative sequence	68 – 82	15	Missing in isoform 2.		VSP_029889
	Natural variant	202	1	L → M. Corresponds to variant rs17762452 [ dbSNP | Ensembl ].		VAR_037553
Experimental info
	Mutagenesis	7	1	S → A: Abolishes phosphorylation by PKG/PRKG1. Ref.1
	Mutagenesis	357	1	N → A: Abolishes GAP activity. Ref.1
	Sequence conflict	285	1	P → L in BAC85912. Ref.2
	Sequence conflict	304	1	K → N in CAF31652. Ref.1
	Sequence conflict	304	1	K → N in CAF31653. Ref.1
	Sequence conflict	726	1	S → P in CAH10514. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Rap1GAP2b) [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 8E7584ADC2DA0B08	FASTA	730	80,056
	10 20 30 40 50 60 MFGRKRSVSF GGFGWIDKTM LASLKVKKQE LANSSDATLP DRPLSPPLTA PPTMKSSEFF 70 80 90 100 110 120 EMLEKMQGIK LEEQKPGPQK NKDDYIPYPS IDEVVEKGGP YPQVILPQFG GYWIEDPENV 130 140 150 160 170 180 GTPTSLGSSI CEEEEEDNLS PNTFGYKLEC KGEARAYRRH FLGKDHLNFY CTGSSLGNLI 190 200 210 220 230 240 LSVKCEEAEG IEYLRVILRS KLKTVHERIP LAGLSKLPSV PQIAKAFCDD AVGLRFNPVL 250 260 270 280 290 300 YPKASQMIVS YDEHEVNNTF KFGVIYQKAR QTLEEELFGN NEESPAFKEF LDLLGDTITL 310 320 330 340 350 360 QDFKGFRGGL DVTHGQTGVE SVYTTFRDRE IMFHVSTKLP FTDGDAQQLQ RKRHIGNDIV 370 380 390 400 410 420 AIIFQEENTP FVPDMIASNF LHAYIVVQVE TPGTETPSYK VSVTAREDVP TFGPPLPSPP 430 440 450 460 470 480 VFQKGPEFRE FLLTKLTNAE NACCKSDKFA KLEDRTRAAL LDNLHDELHA HTQAMLGLGP 490 500 510 520 530 540 EEDKFENGGH GGFLESFKRA IRVRSHSMET MVGGQKKSHS GGIPGSLSGG ISHNSMEVTK 550 560 570 580 590 600 TTFSPPVVAA TVKNQSRSPI KRRSGLFPRL HTGSEGQGDS RARCDSTSST PKTPDGGHSS 610 620 630 640 650 660 QEIKSETSSN PSSPEICPNK EKPFMKLKEN GRAISRSSSS TSSVSSTAGE GEAMEEGDSG 670 680 690 700 710 720 GSQPSTTSPF KQEVFVYSPS PSSESPSLGA AATPIIMSRS PTDAKSRNSP RSNLKFRFDK 730 LSHASSGAGH
	Isoform 2 (Rap1GAP2a) [UniParc]. Checksum: 3A8175583D2C9C3F	FASTA	715	78,380
	10 20 30 40 50 60 MFGRKRSVSF GGFGWIDKTM LASLKVKKQE LANSSDATLP DRPLSPPLTA PPTMKSSEFF 70 80 90 100 110 120 EMLEKMQDDY IPYPSIDEVV EKGGPYPQVI LPQFGGYWIE DPENVGTPTS LGSSICEEEE 130 140 150 160 170 180 EDNLSPNTFG YKLECKGEAR AYRRHFLGKD HLNFYCTGSS LGNLILSVKC EEAEGIEYLR 190 200 210 220 230 240 VILRSKLKTV HERIPLAGLS KLPSVPQIAK AFCDDAVGLR FNPVLYPKAS QMIVSYDEHE 250 260 270 280 290 300 VNNTFKFGVI YQKARQTLEE ELFGNNEESP AFKEFLDLLG DTITLQDFKG FRGGLDVTHG 310 320 330 340 350 360 QTGVESVYTT FRDREIMFHV STKLPFTDGD AQQLQRKRHI GNDIVAIIFQ EENTPFVPDM 370 380 390 400 410 420 IASNFLHAYI VVQVETPGTE TPSYKVSVTA REDVPTFGPP LPSPPVFQKG PEFREFLLTK 430 440 450 460 470 480 LTNAENACCK SDKFAKLEDR TRAALLDNLH DELHAHTQAM LGLGPEEDKF ENGGHGGFLE 490 500 510 520 530 540 SFKRAIRVRS HSMETMVGGQ KKSHSGGIPG SLSGGISHNS MEVTKTTFSP PVVAATVKNQ 550 560 570 580 590 600 SRSPIKRRSG LFPRLHTGSE GQGDSRARCD STSSTPKTPD GGHSSQEIKS ETSSNPSSPE 610 620 630 640 650 660 ICPNKEKPFM KLKENGRAIS RSSSSTSSVS STAGEGEAME EGDSGGSQPS TTSPFKQEVF 670 680 690 700 710 VYSPSPSSES PSLGAAATPI IMSRSPTDAK SRNSPRSNLK FRFDKLSHAS SGAGH
	Isoform 3 (Rap1GAP2c) [UniParc]. Checksum: F90B7B7CBC1F46A6	FASTA	711	77,897
	10 20 30 40 50 60 MLASLKVKKQ ELANSSDATL PDRPLSPPLT APPTMKSSEF FEMLEKMQGI KLEEQKPGPQ 70 80 90 100 110 120 KNKDDYIPYP SIDEVVEKGG PYPQVILPQF GGYWIEDPEN VGTPTSLGSS ICEEEEEDNL 130 140 150 160 170 180 SPNTFGYKLE CKGEARAYRR HFLGKDHLNF YCTGSSLGNL ILSVKCEEAE GIEYLRVILR 190 200 210 220 230 240 SKLKTVHERI PLAGLSKLPS VPQIAKAFCD DAVGLRFNPV LYPKASQMIV SYDEHEVNNT 250 260 270 280 290 300 FKFGVIYQKA RQTLEEELFG NNEESPAFKE FLDLLGDTIT LQDFKGFRGG LDVTHGQTGV 310 320 330 340 350 360 ESVYTTFRDR EIMFHVSTKL PFTDGDAQQL QRKRHIGNDI VAIIFQEENT PFVPDMIASN 370 380 390 400 410 420 FLHAYIVVQV ETPGTETPSY KVSVTAREDV PTFGPPLPSP PVFQKGPEFR EFLLTKLTNA 430 440 450 460 470 480 ENACCKSDKF AKLEDRTRAA LLDNLHDELH AHTQAMLGLG PEEDKFENGG HGGFLESFKR 490 500 510 520 530 540 AIRVRSHSME TMVGGQKKSH SGGIPGSLSG GISHNSMEVT KTTFSPPVVA ATVKNQSRSP 550 560 570 580 590 600 IKRRSGLFPR LHTGSEGQGD SRARCDSTSS TPKTPDGGHS SQEIKSETSS NPSSPEICPN 610 620 630 640 650 660 KEKPFMKLKE NGRAISRSSS STSSVSSTAG EGEAMEEGDS GGSQPSTTSP FKQEVFVYSP 670 680 690 700 710 SPSSESPSLG AAATPIIMSR SPTDAKSRNS PRSNLKFRFD KLSHASSGAG H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rap1GAP2 is a new GTPase-activating protein of Rap1 expressed in human platelets." Schultess J., Danielewski O., Smolenski A.P. Blood 105:3185-3192(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION AT SER-7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF SER-7 AND ASN-357.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cerebellum.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cervix.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-730. Tissue: Brain.
[6]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-544, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558 AND SER-564, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ628446 mRNA. Translation: CAF31652.1. AJ628447 mRNA. Translation: CAF31653.1. AK124640 mRNA. Translation: BAC85912.1. CR627427 mRNA. Translation: CAH10514.1. BC140870 mRNA. Translation: AAI40871.1. BC140871 mRNA. Translation: AAI40872.1. AB028962 mRNA. Translation: BAA82991.1.
IPI	IPI00401939. IPI00855987. IPI00877122.
RefSeq	NP_001093868.1. NM_001100398.1. NP_055900.4. NM_015085.4.
UniGene	Hs.499659.
3D structure databases
HSSP	HSSP built from PDB template 1SRQ based on UniProtKB P47736.
ProteinModelPortal	Q684P5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q684P5. 2 interactions.
STRING	9606.ENSP00000254695.
PTM databases
PhosphoSite	Q684P5.
Polymorphism databases
DMDM	162416269.
Proteomic databases
PaxDb	Q684P5.
PRIDE	Q684P5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000254695; ENSP00000254695; ENSG00000132359. ENST00000366401; ENSP00000389824; ENSG00000132359. ENST00000540393; ENSP00000439688; ENSG00000132359. ENST00000542807; ENSP00000444890; ENSG00000132359.
GeneID	23108.
KEGG	hsa:23108.
UCSC	uc010ckd.3. human. uc010cke.3. human.
Organism-specific databases
CTD	23108.
GeneCards	GC17P002646.
H-InvDB	HIX0013424. HIX0039496.
HGNC	HGNC:29176. RAP1GAP2.
HPA	HPA022148. HPA022896.
neXtProt	NX_Q684P5.
PharmGKB	PA165432528.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG292109.
HOGENOM	HOG000231640.
HOVERGEN	HBG016371.
InParanoid	Q684P5.
OMA	CEEAEGI.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
Bgee	Q684P5.
CleanEx	HS_GARNL4.
Genevestigator	Q684P5.
Family and domain databases
InterPro	IPR000331. Rap_GAP_dom. [Graphical view]
Pfam	PF02145. Rap_GAP. 1 hit. [Graphical view]
PROSITE	PS50085. RAPGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RAP1GAP2. human.
GenomeRNAi	23108.
NextBio	44297.

Entry information

Entry name

RPGP2_HUMAN

Accession

Primary (citable) accession number: Q684P5
Secondary accession number(s): B2RTY5 , Q684P4, Q6AI00, Q6ZVF0, Q9UPW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.