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Q684P5 (RPGP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rap1 GTPase-activating protein 2

Short name=Rap1GAP2
Alternative name(s):
GTPase-activating Rap/Ran-GAP domain-like protein 4
Gene names
Name:RAP1GAP2
Synonyms:GARNL4, KIAA1039, RAP1GA2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length730 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state. Ref.1

Subcellular location

Cytoplasm. Cytoplasmperinuclear region Ref.1.

Tissue specificity

Isoform 1 and isoform 2 are expressed in platelets with isoform 2 being the predominant form. Expressed in lymphocytes, heart, testis and pancreas. Ref.1

Post-translational modification

In vitro phosphorylated by cGMP-dependent protein kinase 1 (cGKI) at Ser-7; the phosphorylation probably does not regulate GAP activity. Ref.1

Sequence similarities

Contains 1 Rap-GAP domain.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q684P5-1)

Also known as: Rap1GAP2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q684P5-2)

Also known as: Rap1GAP2a;

The sequence of this isoform differs from the canonical sequence as follows:
     68-82: Missing.
Isoform 3 (identifier: Q684P5-3)

Also known as: Rap1GAP2c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 730730Rap1 GTPase-activating protein 2
PRO_0000312716

Regions

Domain248 – 464217Rap-GAP
Compositional bias586 – 726141Ser-rich

Amino acid modifications

Modified residue71Phosphoserine; by PKG/PRKG1; in vitro Ref.1
Modified residue451Phosphoserine Ref.7 Ref.8
Modified residue491Phosphothreonine Ref.7 Ref.8
Modified residue5441Phosphoserine Ref.6 Ref.8
Modified residue5581Phosphoserine Ref.9
Modified residue5641Phosphoserine Ref.9

Natural variations

Alternative sequence1 – 1919Missing in isoform 3.
VSP_029888
Alternative sequence68 – 8215Missing in isoform 2.
VSP_029889
Natural variant2021L → M.
Corresponds to variant rs17762452 [ dbSNP | Ensembl ].
VAR_037553

Experimental info

Mutagenesis71S → A: Abolishes phosphorylation by PKG/PRKG1. Ref.1
Mutagenesis3571N → A: Abolishes GAP activity. Ref.1
Sequence conflict2851P → L in BAC85912. Ref.2
Sequence conflict3041K → N in CAF31652. Ref.1
Sequence conflict3041K → N in CAF31653. Ref.1
Sequence conflict7261S → P in CAH10514. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Rap1GAP2b) [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 8E7584ADC2DA0B08

FASTA73080,056
        10         20         30         40         50         60 
MFGRKRSVSF GGFGWIDKTM LASLKVKKQE LANSSDATLP DRPLSPPLTA PPTMKSSEFF 

        70         80         90        100        110        120 
EMLEKMQGIK LEEQKPGPQK NKDDYIPYPS IDEVVEKGGP YPQVILPQFG GYWIEDPENV 

       130        140        150        160        170        180 
GTPTSLGSSI CEEEEEDNLS PNTFGYKLEC KGEARAYRRH FLGKDHLNFY CTGSSLGNLI 

       190        200        210        220        230        240 
LSVKCEEAEG IEYLRVILRS KLKTVHERIP LAGLSKLPSV PQIAKAFCDD AVGLRFNPVL 

       250        260        270        280        290        300 
YPKASQMIVS YDEHEVNNTF KFGVIYQKAR QTLEEELFGN NEESPAFKEF LDLLGDTITL 

       310        320        330        340        350        360 
QDFKGFRGGL DVTHGQTGVE SVYTTFRDRE IMFHVSTKLP FTDGDAQQLQ RKRHIGNDIV 

       370        380        390        400        410        420 
AIIFQEENTP FVPDMIASNF LHAYIVVQVE TPGTETPSYK VSVTAREDVP TFGPPLPSPP 

       430        440        450        460        470        480 
VFQKGPEFRE FLLTKLTNAE NACCKSDKFA KLEDRTRAAL LDNLHDELHA HTQAMLGLGP 

       490        500        510        520        530        540 
EEDKFENGGH GGFLESFKRA IRVRSHSMET MVGGQKKSHS GGIPGSLSGG ISHNSMEVTK 

       550        560        570        580        590        600 
TTFSPPVVAA TVKNQSRSPI KRRSGLFPRL HTGSEGQGDS RARCDSTSST PKTPDGGHSS 

       610        620        630        640        650        660 
QEIKSETSSN PSSPEICPNK EKPFMKLKEN GRAISRSSSS TSSVSSTAGE GEAMEEGDSG 

       670        680        690        700        710        720 
GSQPSTTSPF KQEVFVYSPS PSSESPSLGA AATPIIMSRS PTDAKSRNSP RSNLKFRFDK 

       730 
LSHASSGAGH 

« Hide

Isoform 2 (Rap1GAP2a) [UniParc].

Checksum: 3A8175583D2C9C3F
Show »

FASTA71578,380
Isoform 3 (Rap1GAP2c) [UniParc].

Checksum: F90B7B7CBC1F46A6
Show »

FASTA71177,897

References

« Hide 'large scale' references
[1]"Rap1GAP2 is a new GTPase-activating protein of Rap1 expressed in human platelets."
Schultess J., Danielewski O., Smolenski A.P.
Blood 105:3185-3192(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION AT SER-7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF SER-7 AND ASN-357.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Cerebellum.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Cervix.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-730.
Tissue: Brain.
[6]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558 AND SER-564, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ628446 mRNA. Translation: CAF31652.1.
AJ628447 mRNA. Translation: CAF31653.1.
AK124640 mRNA. Translation: BAC85912.1.
CR627427 mRNA. Translation: CAH10514.1.
BC140870 mRNA. Translation: AAI40871.1.
BC140871 mRNA. Translation: AAI40872.1.
AB028962 mRNA. Translation: BAA82991.1.
RefSeqNP_001093868.1. NM_001100398.1.
NP_055900.4. NM_015085.4.
UniGeneHs.499659.

3D structure databases

ProteinModelPortalQ684P5.
SMRQ684P5. Positions 147-479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116732. 3 interactions.
IntActQ684P5. 2 interactions.
STRING9606.ENSP00000254695.

PTM databases

PhosphoSiteQ684P5.

Polymorphism databases

DMDM162416269.

Proteomic databases

PaxDbQ684P5.
PRIDEQ684P5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254695; ENSP00000254695; ENSG00000132359. [Q684P5-1]
ENST00000366401; ENSP00000389824; ENSG00000132359. [Q684P5-2]
ENST00000540393; ENSP00000439688; ENSG00000132359. [Q684P5-3]
ENST00000542807; ENSP00000444890; ENSG00000132359. [Q684P5-1]
GeneID23108.
KEGGhsa:23108.
UCSCuc010ckd.3. human. [Q684P5-1]
uc010cke.3. human. [Q684P5-2]

Organism-specific databases

CTD23108.
GeneCardsGC17P002680.
H-InvDBHIX0013424.
HIX0039496.
HGNCHGNC:29176. RAP1GAP2.
HPAHPA022148.
HPA022896.
neXtProtNX_Q684P5.
PharmGKBPA165432528.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG292109.
HOGENOMHOG000231640.
HOVERGENHBG016371.
InParanoidQ684P5.
KOK17708.
OMAHNSVEVT.
OrthoDBEOG7WHH8V.
PhylomeDBQ684P5.
TreeFamTF318626.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

BgeeQ684P5.
CleanExHS_GARNL4.
GenevestigatorQ684P5.

Family and domain databases

InterProIPR000331. Rap_GAP_dom.
[Graphical view]
PfamPF02145. Rap_GAP. 1 hit.
[Graphical view]
PROSITEPS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRAP1GAP2. human.
GeneWikiRAP1GAP2.
GenomeRNAi23108.
NextBio44297.
PROQ684P5.

Entry information

Entry nameRPGP2_HUMAN
AccessionPrimary (citable) accession number: Q684P5
Secondary accession number(s): B2RTY5 expand/collapse secondary AC list , Q684P4, Q6AI00, Q6ZVF0, Q9UPW2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM