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Q684P5

- RPGP2_HUMAN

UniProt

Q684P5 - RPGP2_HUMAN

Protein

Rap1 GTPase-activating protein 2

Gene

RAP1GAP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.1 Publication

    GO - Molecular functioni

    1. GTPase activator activity Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    ReactomeiREACT_23898. Rap1 signalling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rap1 GTPase-activating protein 2
    Short name:
    Rap1GAP2
    Alternative name(s):
    GTPase-activating Rap/Ran-GAP domain-like protein 4
    Gene namesi
    Name:RAP1GAP2
    Synonyms:GARNL4, KIAA1039, RAP1GA2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:29176. RAP1GAP2.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmperinuclear region 1 Publication

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: HPA
    3. cytosol Source: Reactome
    4. nuclear membrane Source: HPA
    5. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi7 – 71S → A: Abolishes phosphorylation by PKG/PRKG1. 1 Publication
    Mutagenesisi357 – 3571N → A: Abolishes GAP activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA165432528.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 730730Rap1 GTPase-activating protein 2PRO_0000312716Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei7 – 71Phosphoserine; by PKG/PRKG1; in vitro1 Publication
    Modified residuei45 – 451Phosphoserine2 Publications
    Modified residuei49 – 491Phosphothreonine2 Publications
    Modified residuei544 – 5441Phosphoserine2 Publications
    Modified residuei558 – 5581Phosphoserine1 Publication
    Modified residuei564 – 5641Phosphoserine1 Publication

    Post-translational modificationi

    In vitro phosphorylated by cGMP-dependent protein kinase 1 (cGKI) at Ser-7; the phosphorylation probably does not regulate GAP activity.5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ684P5.
    PaxDbiQ684P5.
    PRIDEiQ684P5.

    PTM databases

    PhosphoSiteiQ684P5.

    Expressioni

    Tissue specificityi

    Isoform 1 and isoform 2 are expressed in platelets with isoform 2 being the predominant form. Expressed in lymphocytes, heart, testis and pancreas.1 Publication

    Gene expression databases

    BgeeiQ684P5.
    CleanExiHS_GARNL4.
    GenevestigatoriQ684P5.

    Organism-specific databases

    HPAiHPA022148.
    HPA022896.

    Interactioni

    Protein-protein interaction databases

    BioGridi116732. 3 interactions.
    IntActiQ684P5. 2 interactions.
    STRINGi9606.ENSP00000254695.

    Structurei

    3D structure databases

    ProteinModelPortaliQ684P5.
    SMRiQ684P5. Positions 147-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini248 – 464217Rap-GAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi586 – 726141Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rap-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG292109.
    HOGENOMiHOG000231640.
    HOVERGENiHBG016371.
    InParanoidiQ684P5.
    KOiK17708.
    OMAiTMVGSQK.
    OrthoDBiEOG7WHH8V.
    PhylomeDBiQ684P5.
    TreeFamiTF318626.

    Family and domain databases

    InterProiIPR000331. Rap_GAP_dom.
    [Graphical view]
    PfamiPF02145. Rap_GAP. 1 hit.
    [Graphical view]
    PROSITEiPS50085. RAPGAP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q684P5-1) [UniParc]FASTAAdd to Basket

    Also known as: Rap1GAP2b

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFGRKRSVSF GGFGWIDKTM LASLKVKKQE LANSSDATLP DRPLSPPLTA    50
    PPTMKSSEFF EMLEKMQGIK LEEQKPGPQK NKDDYIPYPS IDEVVEKGGP 100
    YPQVILPQFG GYWIEDPENV GTPTSLGSSI CEEEEEDNLS PNTFGYKLEC 150
    KGEARAYRRH FLGKDHLNFY CTGSSLGNLI LSVKCEEAEG IEYLRVILRS 200
    KLKTVHERIP LAGLSKLPSV PQIAKAFCDD AVGLRFNPVL YPKASQMIVS 250
    YDEHEVNNTF KFGVIYQKAR QTLEEELFGN NEESPAFKEF LDLLGDTITL 300
    QDFKGFRGGL DVTHGQTGVE SVYTTFRDRE IMFHVSTKLP FTDGDAQQLQ 350
    RKRHIGNDIV AIIFQEENTP FVPDMIASNF LHAYIVVQVE TPGTETPSYK 400
    VSVTAREDVP TFGPPLPSPP VFQKGPEFRE FLLTKLTNAE NACCKSDKFA 450
    KLEDRTRAAL LDNLHDELHA HTQAMLGLGP EEDKFENGGH GGFLESFKRA 500
    IRVRSHSMET MVGGQKKSHS GGIPGSLSGG ISHNSMEVTK TTFSPPVVAA 550
    TVKNQSRSPI KRRSGLFPRL HTGSEGQGDS RARCDSTSST PKTPDGGHSS 600
    QEIKSETSSN PSSPEICPNK EKPFMKLKEN GRAISRSSSS TSSVSSTAGE 650
    GEAMEEGDSG GSQPSTTSPF KQEVFVYSPS PSSESPSLGA AATPIIMSRS 700
    PTDAKSRNSP RSNLKFRFDK LSHASSGAGH 730
    Length:730
    Mass (Da):80,056
    Last modified:December 4, 2007 - v2
    Checksum:i8E7584ADC2DA0B08
    GO
    Isoform 2 (identifier: Q684P5-2) [UniParc]FASTAAdd to Basket

    Also known as: Rap1GAP2a

    The sequence of this isoform differs from the canonical sequence as follows:
         68-82: Missing.

    Show »
    Length:715
    Mass (Da):78,380
    Checksum:i3A8175583D2C9C3F
    GO
    Isoform 3 (identifier: Q684P5-3) [UniParc]FASTAAdd to Basket

    Also known as: Rap1GAP2c

    The sequence of this isoform differs from the canonical sequence as follows:
         1-19: Missing.

    Show »
    Length:711
    Mass (Da):77,897
    Checksum:iF90B7B7CBC1F46A6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti285 – 2851P → L in BAC85912. (PubMed:14702039)Curated
    Sequence conflicti304 – 3041K → N in CAF31652. (PubMed:15632203)Curated
    Sequence conflicti304 – 3041K → N in CAF31653. (PubMed:15632203)Curated
    Sequence conflicti726 – 7261S → P in CAH10514. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti202 – 2021L → M.
    Corresponds to variant rs17762452 [ dbSNP | Ensembl ].
    VAR_037553

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1919Missing in isoform 3. 2 PublicationsVSP_029888Add
    BLAST
    Alternative sequencei68 – 8215Missing in isoform 2. 1 PublicationVSP_029889Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ628446 mRNA. Translation: CAF31652.1.
    AJ628447 mRNA. Translation: CAF31653.1.
    AK124640 mRNA. Translation: BAC85912.1.
    CR627427 mRNA. Translation: CAH10514.1.
    BC140870 mRNA. Translation: AAI40871.1.
    BC140871 mRNA. Translation: AAI40872.1.
    AB028962 mRNA. Translation: BAA82991.1.
    CCDSiCCDS45573.1. [Q684P5-1]
    CCDS45574.1. [Q684P5-2]
    RefSeqiNP_001093868.1. NM_001100398.1. [Q684P5-2]
    NP_055900.4. NM_015085.4. [Q684P5-1]
    XP_006721540.1. XM_006721477.1. [Q684P5-3]
    UniGeneiHs.499659.

    Genome annotation databases

    EnsembliENST00000254695; ENSP00000254695; ENSG00000132359. [Q684P5-1]
    ENST00000366401; ENSP00000389824; ENSG00000132359. [Q684P5-2]
    ENST00000540393; ENSP00000439688; ENSG00000132359. [Q684P5-3]
    ENST00000542807; ENSP00000444890; ENSG00000132359. [Q684P5-1]
    GeneIDi23108.
    KEGGihsa:23108.
    UCSCiuc010ckd.3. human. [Q684P5-1]
    uc010cke.3. human. [Q684P5-2]

    Polymorphism databases

    DMDMi162416269.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ628446 mRNA. Translation: CAF31652.1 .
    AJ628447 mRNA. Translation: CAF31653.1 .
    AK124640 mRNA. Translation: BAC85912.1 .
    CR627427 mRNA. Translation: CAH10514.1 .
    BC140870 mRNA. Translation: AAI40871.1 .
    BC140871 mRNA. Translation: AAI40872.1 .
    AB028962 mRNA. Translation: BAA82991.1 .
    CCDSi CCDS45573.1. [Q684P5-1 ]
    CCDS45574.1. [Q684P5-2 ]
    RefSeqi NP_001093868.1. NM_001100398.1. [Q684P5-2 ]
    NP_055900.4. NM_015085.4. [Q684P5-1 ]
    XP_006721540.1. XM_006721477.1. [Q684P5-3 ]
    UniGenei Hs.499659.

    3D structure databases

    ProteinModelPortali Q684P5.
    SMRi Q684P5. Positions 147-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 116732. 3 interactions.
    IntActi Q684P5. 2 interactions.
    STRINGi 9606.ENSP00000254695.

    PTM databases

    PhosphoSitei Q684P5.

    Polymorphism databases

    DMDMi 162416269.

    Proteomic databases

    MaxQBi Q684P5.
    PaxDbi Q684P5.
    PRIDEi Q684P5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000254695 ; ENSP00000254695 ; ENSG00000132359 . [Q684P5-1 ]
    ENST00000366401 ; ENSP00000389824 ; ENSG00000132359 . [Q684P5-2 ]
    ENST00000540393 ; ENSP00000439688 ; ENSG00000132359 . [Q684P5-3 ]
    ENST00000542807 ; ENSP00000444890 ; ENSG00000132359 . [Q684P5-1 ]
    GeneIDi 23108.
    KEGGi hsa:23108.
    UCSCi uc010ckd.3. human. [Q684P5-1 ]
    uc010cke.3. human. [Q684P5-2 ]

    Organism-specific databases

    CTDi 23108.
    GeneCardsi GC17P002680.
    H-InvDB HIX0013424.
    HIX0039496.
    HGNCi HGNC:29176. RAP1GAP2.
    HPAi HPA022148.
    HPA022896.
    neXtProti NX_Q684P5.
    PharmGKBi PA165432528.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG292109.
    HOGENOMi HOG000231640.
    HOVERGENi HBG016371.
    InParanoidi Q684P5.
    KOi K17708.
    OMAi TMVGSQK.
    OrthoDBi EOG7WHH8V.
    PhylomeDBi Q684P5.
    TreeFami TF318626.

    Enzyme and pathway databases

    Reactomei REACT_23898. Rap1 signalling.

    Miscellaneous databases

    ChiTaRSi RAP1GAP2. human.
    GeneWikii RAP1GAP2.
    GenomeRNAii 23108.
    NextBioi 44297.
    PROi Q684P5.

    Gene expression databases

    Bgeei Q684P5.
    CleanExi HS_GARNL4.
    Genevestigatori Q684P5.

    Family and domain databases

    InterProi IPR000331. Rap_GAP_dom.
    [Graphical view ]
    Pfami PF02145. Rap_GAP. 1 hit.
    [Graphical view ]
    PROSITEi PS50085. RAPGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rap1GAP2 is a new GTPase-activating protein of Rap1 expressed in human platelets."
      Schultess J., Danielewski O., Smolenski A.P.
      Blood 105:3185-3192(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION AT SER-7, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF SER-7 AND ASN-357.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Cerebellum.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Cervix.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Kikuno R., Nagase T., Ishikawa K., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 6:197-205(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 287-730.
      Tissue: Brain.
    6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-49, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-49 AND SER-544, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-558 AND SER-564, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRPGP2_HUMAN
    AccessioniPrimary (citable) accession number: Q684P5
    Secondary accession number(s): B2RTY5
    , Q684P4, Q6AI00, Q6ZVF0, Q9UPW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3