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Protein

E3 SUMO-protein ligase SIZ1

Gene

SIZ1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses (PubMed:15894620). Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as positive regulator of drought stress tolerance. Acts as floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5.9 Publications

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri112 – 168PHD-typeAdd BLAST57
Zinc fingeri348 – 425SP-RING-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • SUMO transferase activity Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell division Source: TAIR
  • cell growth Source: TAIR
  • cellular response to extracellular stimulus Source: InterPro
  • cellular response to phosphate starvation Source: TAIR
  • defense response Source: UniProtKB-KW
  • detection of phosphate ion Source: TAIR
  • developmental growth Source: UniProtKB
  • embryo sac development Source: TAIR
  • flower development Source: UniProtKB-KW
  • heat acclimation Source: UniProtKB
  • negative regulation of flower development Source: TAIR
  • negative regulation of systemic acquired resistance Source: UniProtKB
  • plant ovule development Source: TAIR
  • pollen tube guidance Source: TAIR
  • protein sumoylation Source: UniProtKB
  • regulation of abscisic acid-activated signaling pathway Source: TAIR
  • regulation of growth Source: TAIR
  • regulation of nitrate assimilation Source: TAIR
  • regulation of response to water deprivation Source: TAIR
  • regulation of salicylic acid metabolic process Source: TAIR
  • response to freezing Source: TAIR
  • response to water deprivation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Flowering, Plant defense, Stress response, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-3108214. SUMOylation of DNA damage response and repair proteins.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5696395. Formation of Incision Complex in GG-NER.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase SIZ11 Publication (EC:6.3.2.-Curated)
Gene namesi
Name:SIZ11 Publication
Ordered Locus Names:At5g60410Imported
ORF Names:MUF9.5Imported, MUF9.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G60410.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Dwarf phenotype. Heat-sensitive phenotype. Early flowering under short day. Elevated level of salicylic acid (SA), increased expression of pathogenesis-related (PR) genes and increased resistance to the bacterial pathogen P.syringae. ABA hypersensitivity during seed germination primary root growth. Exaggerated prototypical Pi-starvation responses, including increased root-shoot mass ratio and greater anthocyanin accumulation (PubMed:15894620).7 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100K → R: Strongly reduces autosumoylation; when associated with R-488. 1 Publication1
Mutagenesisi488K → R: Strongly reduces autosumoylation; when associated with R-100. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189841 – 884E3 SUMO-protein ligase SIZ1Add BLAST884

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki488Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Autosumoylated at Lys-100 and Lys-488.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ680Q4.

PTM databases

iPTMnetiQ680Q4.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ680Q4. baseline and differential.
GenevisibleiQ680Q4. AT.

Interactioni

Subunit structurei

Interacts (via PHD domain) with SCE1, GTE3 and GTE5.1 Publication

Protein-protein interaction databases

BioGridi21407. 10 interactors.
STRINGi3702.AT5G60410.2.

Structurei

Secondary structure

1884
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi106 – 108Combined sources3
Beta strandi126 – 128Combined sources3
Turni132 – 134Combined sources3
Beta strandi137 – 139Combined sources3
Helixi140 – 143Combined sources4
Turni148 – 150Combined sources3
Helixi163 – 167Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WEWNMR-A104-168[»]
ProteinModelPortaliQ680Q4.
SMRiQ680Q4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ680Q4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 45SAPPROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi336 – 340Poly-Gly5

Domaini

The PHD-type zinc finger mediates interaction with SCE1, GTE3 and GTE5 and is required for E3 activity.1 Publication

Sequence similaritiesi

Belongs to the PIAS family.Curated
Contains 1 PHD-type zinc finger.Curated
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri112 – 168PHD-typeAdd BLAST57
Zinc fingeri348 – 425SP-RING-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2169. Eukaryota.
ENOG410XQ2E. LUCA.
HOGENOMiHOG000029457.
InParanoidiQ680Q4.
OMAiQWHAPDG.
PhylomeDBiQ680Q4.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR003034. SAP_dom.
IPR031141. SIZ1_plant.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR004181. Znf_MIZ.
IPR001965. Znf_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF34. PTHR10782:SF34. 2 hits.
PfamiPF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50800. SAP. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q680Q4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA
60 70 80 90 100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK
110 120 130 140 150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM
160 170 180 190 200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ
210 220 230 240 250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG
260 270 280 290 300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV
310 320 330 340 350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI
360 370 380 390 400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW
410 420 430 440 450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK
460 470 480 490 500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK
510 520 530 540 550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD
560 570 580 590 600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV
610 620 630 640 650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA
660 670 680 690 700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH
710 720 730 740 750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR
760 770 780 790 800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG
810 820 830 840 850
DPATTNGINS SHQMSTREGS MDTTTETASL LLGMNDSRQD KAKKQRSDNP
860 870 880
FSFPRQKRSN NEQDHQTRHR SLNKICIILC AGKN
Note: No experimental confirmation available.
Length:884
Mass (Da):97,034
Last modified:August 30, 2005 - v2
Checksum:i5E44EA57FE896113
GO
Isoform 2 (identifier: Q680Q4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     828-832: ASLLL → GLFPV
     833-884: Missing.

Note: No experimental confirmation available.
Show »
Length:832
Mass (Da):90,970
Checksum:i8C5DD47141D7E92F
GO
Isoform 3 (identifier: Q680Q4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     860-873: NNEQDHQTRHRSLN → VRPRMYLSIDSDSE
     874-884: Missing.

Show »
Length:873
Mass (Da):95,795
Checksum:i0C9BD3F7F66AA3ED
GO
Isoform 4 (identifier: Q680Q4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     860-884: NNEQDHQTRHRSLNKICIILCAGKN → VRPRMYLSIDSDSETMNRIIRQDTGV

Note: No experimental confirmation available.
Show »
Length:885
Mass (Da):97,181
Checksum:iCBD103E9A048FD1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335I → S in BAD94301 (Ref. 5) Curated1
Sequence conflicti335I → S in BAD43867 (Ref. 5) Curated1
Sequence conflicti436E → D in BAD43867 (Ref. 5) Curated1
Sequence conflicti785D → G in BAD43867 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015483828 – 832ASLLL → GLFPV in isoform 2. 2 Publications5
Alternative sequenceiVSP_015484833 – 884Missing in isoform 2. 2 PublicationsAdd BLAST52
Alternative sequenceiVSP_015485860 – 884NNEQD…CAGKN → VRPRMYLSIDSDSETMNRII RQDTGV in isoform 4. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_015486860 – 873NNEQD…HRSLN → VRPRMYLSIDSDSE in isoform 3. 3 PublicationsAdd BLAST14
Alternative sequenceiVSP_015487874 – 884Missing in isoform 3. 3 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700572 mRNA. Translation: AAU00414.1.
AB011483 Genomic DNA. Translation: BAB08225.1.
CP002688 Genomic DNA. Translation: AED97324.1.
CP002688 Genomic DNA. Translation: AED97325.1.
CP002688 Genomic DNA. Translation: AED97326.1.
CP002688 Genomic DNA. Translation: AED97327.1.
CP002688 Genomic DNA. Translation: AED97328.1.
AY139752 mRNA. Translation: AAM98074.1.
BT004542 mRNA. Translation: AAO42788.1.
AK175813 mRNA. Translation: BAD43576.1.
AK221190 mRNA. Translation: BAD95283.1.
AK220973 mRNA. Translation: BAD94544.1.
AK221249 mRNA. Translation: BAD93882.1.
AK221292 mRNA. Translation: BAD94016.1.
AK220890 mRNA. Translation: BAD94301.1.
AK176104 mRNA. Translation: BAD43867.1.
AK316983 mRNA. Translation: BAH19679.1.
RefSeqiNP_001032108.1. NM_001037031.1. [Q680Q4-2]
NP_001032109.2. NM_001037032.2. [Q680Q4-1]
NP_001119465.1. NM_001125993.1. [Q680Q4-4]
NP_200849.2. NM_125434.4. [Q680Q4-3]
NP_974969.1. NM_203240.3. [Q680Q4-4]
UniGeneiAt.43746.

Genome annotation databases

EnsemblPlantsiAT5G60410.4; AT5G60410.4; AT5G60410. [Q680Q4-1]
GeneIDi836163.
GrameneiAT5G60410.4; AT5G60410.4; AT5G60410.
KEGGiath:AT5G60410.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700572 mRNA. Translation: AAU00414.1.
AB011483 Genomic DNA. Translation: BAB08225.1.
CP002688 Genomic DNA. Translation: AED97324.1.
CP002688 Genomic DNA. Translation: AED97325.1.
CP002688 Genomic DNA. Translation: AED97326.1.
CP002688 Genomic DNA. Translation: AED97327.1.
CP002688 Genomic DNA. Translation: AED97328.1.
AY139752 mRNA. Translation: AAM98074.1.
BT004542 mRNA. Translation: AAO42788.1.
AK175813 mRNA. Translation: BAD43576.1.
AK221190 mRNA. Translation: BAD95283.1.
AK220973 mRNA. Translation: BAD94544.1.
AK221249 mRNA. Translation: BAD93882.1.
AK221292 mRNA. Translation: BAD94016.1.
AK220890 mRNA. Translation: BAD94301.1.
AK176104 mRNA. Translation: BAD43867.1.
AK316983 mRNA. Translation: BAH19679.1.
RefSeqiNP_001032108.1. NM_001037031.1. [Q680Q4-2]
NP_001032109.2. NM_001037032.2. [Q680Q4-1]
NP_001119465.1. NM_001125993.1. [Q680Q4-4]
NP_200849.2. NM_125434.4. [Q680Q4-3]
NP_974969.1. NM_203240.3. [Q680Q4-4]
UniGeneiAt.43746.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WEWNMR-A104-168[»]
ProteinModelPortaliQ680Q4.
SMRiQ680Q4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21407. 10 interactors.
STRINGi3702.AT5G60410.2.

PTM databases

iPTMnetiQ680Q4.

Proteomic databases

PaxDbiQ680Q4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G60410.4; AT5G60410.4; AT5G60410. [Q680Q4-1]
GeneIDi836163.
GrameneiAT5G60410.4; AT5G60410.4; AT5G60410.
KEGGiath:AT5G60410.

Organism-specific databases

TAIRiAT5G60410.

Phylogenomic databases

eggNOGiKOG2169. Eukaryota.
ENOG410XQ2E. LUCA.
HOGENOMiHOG000029457.
InParanoidiQ680Q4.
OMAiQWHAPDG.
PhylomeDBiQ680Q4.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiR-ATH-3108214. SUMOylation of DNA damage response and repair proteins.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

EvolutionaryTraceiQ680Q4.
PROiQ680Q4.

Gene expression databases

ExpressionAtlasiQ680Q4. baseline and differential.
GenevisibleiQ680Q4. AT.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR003034. SAP_dom.
IPR031141. SIZ1_plant.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR004181. Znf_MIZ.
IPR001965. Znf_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10782:SF34. PTHR10782:SF34. 2 hits.
PfamiPF02037. SAP. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50800. SAP. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIZ1_ARATH
AccessioniPrimary (citable) accession number: Q680Q4
Secondary accession number(s): B9DG12
, Q56YS2, Q56ZS1, Q67ZL3, Q9FKK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.