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Protein

Proteinaceous RNase P 2

Gene

PRORP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease RNase P responsible for the 5' maturation of tRNA precursors (PubMed:22549728, PubMed:26655022). Preferentially binds precursor tRNAs containing short 5' leaders and 3' trailers (PubMed:26655022). Also involved in the maturation of mRNA and small nucleolar RNA (snoRNA) (PubMed:22549728).2 Publications

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.2 Publications

Cofactori

Mg2+1 PublicationNote: Binds 2 Mg2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi281ZincCombined sources1 Publication1
Metal bindingi284ZincCombined sources1 Publication1
Metal bindingi343Magnesium 1; catalytic1 Publication1
Metal bindingi421Magnesium 1; catalytic1 Publication1
Metal bindingi422Magnesium 2; catalytic1 Publication1
Metal bindingi440Magnesium 2; catalytic1 Publication1
Metal bindingi494Zinc; via tele nitrogenCombined sources1 Publication1
Metal bindingi511ZincCombined sources1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ribonuclease P activity Source: TAIR

GO - Biological processi

  • mRNA processing Source: TAIR
  • snoRNA processing Source: TAIR
  • tRNA 5'-leader removal Source: TAIR

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processtRNA processing
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinaceous RNase P 2 (EC:3.1.26.52 Publications)
Gene namesi
Name:PRORP2
Ordered Locus Names:At2g16650
ORF Names:T24I21.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G16650
TAIRilocus:2059824 AT2G16650

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due to the redundancy with PRORP3. Prorp2 and prorp3 double mutant is lethal.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343D → A: Abolishes ribonuclease activity. 1 Publication1
Mutagenesisi421 – 422DD → AA: Loss of activity. 1 Publication2
Mutagenesisi421D → A: Abolishes ribonuclease activity. 1 Publication1
Mutagenesisi422D → A: Abolishes ribonuclease activity. 1 Publication1
Mutagenesisi440D → A: Abolishes ribonuclease activity. 1 Publication1
Mutagenesisi445H → A: Abolishes ribonuclease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004202731 – 528Proteinaceous RNase P 2Add BLAST528

Proteomic databases

PaxDbiQ680B9
PRIDEiQ680B9

PTM databases

iPTMnetiQ680B9

Expressioni

Gene expression databases

ExpressionAtlasiQ680B9 baseline and differential
GenevisibleiQ680B9 AT

Interactioni

Subunit structurei

Monomer; forms dimers in crystallo but monomers in solution (PubMed:26655022).1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G16650.1

Structurei

Secondary structure

1528
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 43Combined sources15
Helixi46 – 59Combined sources14
Helixi67 – 81Combined sources15
Turni83 – 85Combined sources3
Helixi86 – 101Combined sources16
Turni102 – 104Combined sources3
Helixi109 – 121Combined sources13
Helixi125 – 138Combined sources14
Helixi146 – 158Combined sources13
Helixi162 – 175Combined sources14
Helixi181 – 193Combined sources13
Helixi197 – 210Combined sources14
Beta strandi212 – 214Combined sources3
Helixi216 – 226Combined sources11
Helixi229 – 234Combined sources6
Helixi243 – 252Combined sources10
Beta strandi264 – 266Combined sources3
Beta strandi269 – 273Combined sources5
Beta strandi279 – 281Combined sources3
Turni282 – 284Combined sources3
Helixi295 – 310Combined sources16
Helixi323 – 335Combined sources13
Beta strandi339 – 343Combined sources5
Helixi344 – 349Combined sources6
Beta strandi350 – 352Combined sources3
Beta strandi354 – 356Combined sources3
Helixi361 – 375Combined sources15
Beta strandi382 – 386Combined sources5
Helixi387 – 395Combined sources9
Helixi397 – 409Combined sources13
Beta strandi411 – 415Combined sources5
Beta strandi417 – 419Combined sources3
Helixi423 – 431Combined sources9
Beta strandi435 – 437Combined sources3
Helixi445 – 450Combined sources6
Helixi453 – 462Combined sources10
Beta strandi463 – 467Combined sources5
Beta strandi469 – 471Combined sources3
Beta strandi474 – 476Combined sources3
Beta strandi489 – 491Combined sources3
Beta strandi493 – 497Combined sources5
Beta strandi509 – 513Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DIZX-ray3.20A/B1-528[»]
5FT9X-ray3.05A/B2-528[»]
ProteinModelPortaliQ680B9
SMRiQ680B9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati29 – 64PPR 1Add BLAST36
Repeati72 – 107PPR 2Add BLAST36
Repeati108 – 142PPR 3Add BLAST35
Repeati145 – 179PPR 4Add BLAST35
Domaini275 – 511PRORPAdd BLAST237

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi21 – 24Poly-Lys4

Sequence similaritiesi

Belongs to the PPR family. P subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1347 Eukaryota
COG0534 LUCA
HOGENOMiHOG000006117
InParanoidiQ680B9
KOiK18213
OMAiEWFCGEK
OrthoDBiEOG093605S6
PhylomeDBiQ680B9

Family and domain databases

Gene3Di1.25.40.10, 2 hits
InterProiView protein in InterPro
IPR033443 PPR_long
IPR031595 PRORP_C
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF17177 PPR_long, 1 hit
PF16953 PRORP, 1 hit

Sequencei

Sequence statusi: Complete.

Q680B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASDQHRSR RHDESSSRPN KKKKVSRNPE TNLLFNLNSC SKSKDLSAAL
60 70 80 90 100
ALYDAAITSS EVRLSQQHFQ TLLYLCSASI TDISLQYLAI DRGFEIFDRM
110 120 130 140 150
VSSGISPNEA SVTSVARLAA AKGNGDYAFK VVKEFVSVGG VSIPRLRTYA
160 170 180 190 200
PALLCFCEKL EAEKGYEVEE HMEAAGIALE EAEISALLKV SAATGRENKV
210 220 230 240 250
YRYLHKLREY VGCVSEETLK IIEEWFCGEK AGEVGDNGIG SDVGMLREAV
260 270 280 290 300
LNNGGGWHGH GWVGEGKWTV KKGNVSSTGR CLSCSEQLAC VDTNEVETQK
310 320 330 340 350
FVDSLVALAM DRKTKMNSCE TNVVFSEFQD WLEKHGDYEA IVDGANIGLY
360 370 380 390 400
QQNFVDGSFS LSQLESVMKE LYRESGNNKW PLILLHKRRV KTLLENPTHR
410 420 430 440 450
NLVEEWISNG VLYATPPGSN DDWYWLYAAA KLKCLLVTND EMRDHIFELL
460 470 480 490 500
GSTFFQKWKE RHQVRYTFVK GNLKLEMPSP FSVVIQESEK GSWHFPVSCE
510 520
NNEESSRTWM CISRQSILDS PKSNGKIP
Length:528
Mass (Da):59,228
Last modified:October 11, 2004 - v1
Checksum:iE9CE36AE22686ABF
GO

Sequence cautioni

The sequence AAD24622 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005825 Genomic DNA Translation: AAD24622.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC06522.1
AK175948 mRNA Translation: BAD43711.1
PIRiE84542
RefSeqiNP_179256.3, NM_127217.4
UniGeneiAt.29161

Genome annotation databases

EnsemblPlantsiAT2G16650.1; AT2G16650.1; AT2G16650
GeneIDi816166
GrameneiAT2G16650.1; AT2G16650.1; AT2G16650
KEGGiath:AT2G16650

Similar proteinsi

Entry informationi

Entry nameiPRRP2_ARATH
AccessioniPrimary (citable) accession number: Q680B9
Secondary accession number(s): Q9SLF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health