Q67ZU1 (LIP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triacylglycerol lipase 2
EC=3.1.1.3

Gene names

Name:	LIP2
Ordered Locus Names:	At5g14180
ORF Names:	MUA22.18, MUA22.19

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Triacylglycerol (TAG) lipase. May be involved for TAG storage breakdown during seed germination By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.
Sequence caution	The sequence AAK82499.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB08297.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB08298.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	defense response to insect Inferred from mutant phenotype. Source: TAIR lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 418	387	Triacylglycerol lipase 2		PRO_0000234337
Sites
	Active site	190	1	Nucleophile By similarity
	Active site	360	1	Charge relay system By similarity
	Active site	393	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	286	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	191	1	L → Q Ref.5
	Sequence conflict	213	1	A → G in AAQ84586. Ref.4
	Sequence conflict	242	1	S → A in AAQ84586. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q67ZU1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 613F11551D34A6D7	FASTA	418	46,048
	10 20 30 40 50 60 MAGSVMVPSV SIGLALSVLI FFALSLKTLE ARGTFGRLAG QPPQRTAAGG ICASSVHIFG 70 80 90 100 110 120 YKCEEHDVVT QDGYILNMQR IPEGRAGAVA GDGGKRQPVL IQHGILVDGM SWLLNPADQN 130 140 150 160 170 180 LPLILADQGF DVWMGNTRGT RFSRRHKYLN PSQRAFWNWT WDELVSYDLP AMFDHIHGLT 190 200 210 220 230 240 GQKIHYLGHS LGTLIGFASF SEKGLVDQVR SAAMLSPVAY LSHMTTVIGD IAAKTFLAEA 250 260 270 280 290 300 TSILGWPEFN PKSGLVGDFI KAICLKAGID CYDLVSVITG KNCCLNASTI DLFLANEPQS 310 320 330 340 350 360 TSTKNMIHLA QTVRDKELRK YNYGSSDRNI KHYGQAIPPA YNISAIPHEL PLFFSYGGLD 370 380 390 400 410 SLADVKDVEF LLDQFKYHDI DKMNVQFVKD YAHADFIMGV TAKDVVYNQV ATFFKRQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Sterol (triglyceride) ester hydrolase from Arabidopsis thaliana." Benveniste P., Noiriel A., Bouvier-Nave P., Schaller H. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 110-418. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 191-418. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007650 Genomic DNA. Translation: BAB08297.1. Sequence problems. AB007650 Genomic DNA. Translation: BAB08298.1. Sequence problems. CP002688 Genomic DNA. Translation: AED91996.1. AK175707 mRNA. Translation: BAD43470.1. AK176026 mRNA. Translation: BAD43789.1. AY320231 mRNA. Translation: AAQ84586.1. AY048236 mRNA. Translation: AAK82499.1. Different initiation. AY091711 mRNA. Translation: AAM10310.1.
IPI	IPI00517577.
RefSeq	NP_568295.2. NM_121422.3.
UniGene	At.6513.
3D structure databases
ProteinModelPortal	Q67ZU1.
SMR	Q67ZU1. Positions 34-417.
ModBase	Search...
Protein-protein interaction databases
STRING	Q67ZU1.
Protein family/group databases
MEROPS	S33.A64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G14180.1; AT5G14180.1; AT5G14180.
GeneID	831268.
GenomeReviews	Gene locus AT5G14180 in contig BA000015_GR.
KEGG	ath:AT5G14180.
NMPDR	fig|3702.1.peg.23548.
Organism-specific databases
TAIR	At5g14180.
Phylogenomic databases
eggNOG	KOG2624.
GeneTree	EPGT00050000006109.
HOGENOM	HBG383498.
InParanoid	Q67ZU1.
OMA	NCCLNAS.
PhylomeDB	Q67ZU1.
ProtClustDB	CLSN2681046.
Gene expression databases
Genevestigator	Q67ZU1.
GermOnline	AT5G14180. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR006693. AB_hydrolase_lipase. [Graphical view]
Pfam	PF04083. Abhydro_lipase. 1 hit. PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP2_ARATH

Accession

Primary (citable) accession number: Q67ZU1
Secondary accession number(s): Q681B0 , Q6VY31, Q94AE7, Q9FMT2, Q9FMT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 11, 2004
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program