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Protein

Tubulin-folding cofactor B

Gene

TFCB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in control of cell division. Regulates probably the availability of alpha-tubulin for dimerization of alpha-/beta-tubulin, which is required for proper microtubule biogenesis. Decreased expression of TFCB results in enlarged mesophyll cells and leaf epidermal cells with bulged nuclei, increased ploidy and increased numbers of spindles and phragmoplasts.1 Publication

GO - Biological processi

  • cell division Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-folding cofactor B
Short name:
AtTFCB
Alternative name(s):
Protein EMBRYO DEFECTIVE 2804
Gene namesi
Name:TFCB
Synonyms:EMB2804, TBCB
Ordered Locus Names:At3g10220
ORF Names:F14P13.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G10220
TAIRilocus:2076224 AT3G10220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Embryo lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004234991 – 243Tubulin-folding cofactor BAdd BLAST243

Proteomic databases

PaxDbiQ67Z52
PRIDEiQ67Z52

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ67Z52 baseline and differential
GenevisibleiQ67Z52 AT

Interactioni

Subunit structurei

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Interacts with TUBA6.1 Publication

Protein-protein interaction databases

STRINGi3702.AT3G10220.1

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 20Combined sources7
Beta strandi28 – 32Combined sources5
Helixi38 – 49Combined sources12
Beta strandi55 – 61Combined sources7
Beta strandi63 – 65Combined sources3
Helixi73 – 75Combined sources3
Helixi78 – 81Combined sources4
Beta strandi88 – 93Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KJ6NMR-A10-96[»]
ProteinModelPortaliQ67Z52
SMRiQ67Z52
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ67Z52

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini181 – 223CAP-GlyPROSITE-ProRule annotationAdd BLAST43

Sequence similaritiesi

Belongs to the TBCB family.Curated

Phylogenomic databases

eggNOGiKOG3206 Eukaryota
ENOG410YKGB LUCA
HOGENOMiHOG000209180
InParanoidiQ67Z52
KOiK17262
OMAiYNEEEMQ
OrthoDBiEOG09360JLI
PhylomeDBiQ67Z52

Family and domain databases

Gene3Di2.30.30.190, 1 hit
InterProiView protein in InterPro
IPR036859 CAP-Gly_dom_sf
IPR000938 CAP-Gly_domain
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF01302 CAP_GLY, 1 hit
PF14560 Ubiquitin_2, 1 hit
SMARTiView protein in SMART
SM01052 CAP_GLY, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF74924 SSF74924, 1 hit
PROSITEiView protein in PROSITE
PS50245 CAP_GLY_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q67Z52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSRLQLEG DDSVHLHITH ANLKSFSADA RFSPQMSVEA VKEKLWKKCG
60 70 80 90 100
TSVNSMALEL YDDSGSKVAV LSDDSRPLGF FSPFDGFRLH IIDLDPSSVT
110 120 130 140 150
TGGWLEDTSL VEKYNISEED YAKRTDSFRK FKEKRVSQNP VAAEAKTKEN
160 170 180 190 200
YMEDLCANIK VGDRCQVEPG EKRGMVKYVG RAESLGPGYW VGIQYDEPLG
210 220 230 240
KHDGMVKGTR FFECPRLQGG MVRPDKVKVG DYPERDPFEE DEI
Length:243
Mass (Da):27,331
Last modified:October 11, 2004 - v1
Checksum:iE4685B37CFD9DC16
GO

Sequence cautioni

The sequence AAF02820 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43E → G in BAD44467 (Ref. 4) Curated1
Sequence conflicti75S → F in AAM22958 (PubMed:11959844).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF486849 mRNA Translation: AAM22958.1
AC009400 Genomic DNA Translation: AAF02820.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74873.1
AK176183 mRNA Translation: BAD43946.1
AK176266 mRNA Translation: BAD44029.1
AK176704 mRNA Translation: BAD44467.1
RefSeqiNP_187633.2, NM_111857.5
UniGeneiAt.1532
At.71154
At.71830

Genome annotation databases

EnsemblPlantsiAT3G10220.1; AT3G10220.1; AT3G10220
GeneIDi820184
GrameneiAT3G10220.1; AT3G10220.1; AT3G10220
KEGGiath:AT3G10220

Similar proteinsi

Entry informationi

Entry nameiTBCB_ARATH
AccessioniPrimary (citable) accession number: Q67Z52
Secondary accession number(s): Q67XW4, Q8L5R5, Q9SS34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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