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Protein

Inorganic pyrophosphatase 1

Gene

PS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific cleavage of pyrophosphate.1 Publication

Catalytic activityi

Diphosphate + H2O = 2 phosphate.1 Publication

Cofactori

Mg2+1 Publication, Fe2+1 Publication, Ni2+1 Publication, Co2+1 Publication, Mn2+1 PublicationNote: Magnesium. Can also use iron, nickel, cobalt and manganese, but not zinc ions.1 Publication

Kineticsi

  1. KM=38.8 µM for pyrophosphate PPi (at 37 degrees Celsius)1 Publication
  1. Vmax=0.3 nmol/min/µg enzyme with PPi as substrate (at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8-8.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19NucleophileCurated1
Metal bindingi19MagnesiumBy similarity1
Active sitei21Proton donorBy similarity1
Metal bindingi21MagnesiumBy similarity1
Binding sitei30SubstrateBy similarity1
Binding sitei105SubstrateBy similarity1
Metal bindingi190MagnesiumBy similarity1

GO - Molecular functioni

  • inorganic diphosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphatase activity Source: GO_Central
  • pyrophosphatase activity Source: TAIR

GO - Biological processi

  • cellular response to phosphate starvation Source: UniProtKB
  • protein tetramerization Source: UniProtKB

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G73010-MONOMER.
BRENDAi3.6.1.1. 399.
ReactomeiR-ATH-1483191. Synthesis of PC.
R-ATH-1483213. Synthesis of PE.

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase 1 (EC:3.6.1.1)
Short name:
AtPPsPase1
Short name:
PPi phosphatase 1
Short name:
Pyrophosphate-specific phosphatase 1
Alternative name(s):
Protein PHOSPHATE STARVATION-INDUCED GENE 2
Short name:
AtPS2
Gene namesi
Name:PS2
Ordered Locus Names:At1g73010
ORF Names:F3N23.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G73010.
TAIRilocus:2032748. AT1G73010.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19D → H: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004042561 – 295Inorganic pyrophosphatase 1Add BLAST295

Proteomic databases

PaxDbiQ67YC0.

Expressioni

Inductioni

Strongly induced upon phosphate (Pi) starvation.2 Publications

Gene expression databases

GenevisibleiQ67YC0. AT.

Interactioni

Subunit structurei

Tetramer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G73010.1.

Structurei

3D structure databases

ProteinModelPortaliQ67YC0.
SMRiQ67YC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 13Poly-Asn10

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiKOG3120. Eukaryota.
ENOG4111NAQ. LUCA.
HOGENOMiHOG000238030.
InParanoidiQ67YC0.
KOiK13248.
OMAiSPGMGDL.
OrthoDBiEOG09360K2I.
PhylomeDBiQ67YC0.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR023214. HAD-like_dom.
IPR016965. Pase_PHOSPHO-typ.
IPR006384. PyrdxlP_Pase-rel.
PANTHERiPTHR20889. PTHR20889. 1 hit.
PfamiView protein in Pfam
PF06888. Put_Phosphatase. 1 hit.
PIRSFiPIRSF031051. PyrdxlP_Pase_PHOSPHO2. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01489. DKMTPPase-SF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67YC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYNSNSNNN NNNIVVVFDF DKTIIDVDSD NWVIDELGFT DLFNQLLPTM
60 70 80 90 100
PWNTLMDRMM KELHDQGKTI EEIKQVLRTI PIHPRVVPAI KSAHDLGCEL
110 120 130 140 150
RIVSDANMFF IETIVEHLGI SELFSEINSN PGYVDERGTL KISPYHDFTK
160 170 180 190 200
SPHSCSCGTC PPNMCKGLII ERIQQSLAKE GKKKMIYLGD GAGDYCPSLK
210 220 230 240 250
LNTEDYVMPR KNFPVWDLIS QNPMLIKAAI REWTDGQSME MILIGTIEEI
260 270 280 290
RLEEEKEKML TSAENNCKMQ TISIGINNVH HEPILPRALR VSQSS
Length:295
Mass (Da):33,515
Last modified:October 11, 2004 - v1
Checksum:i3B353D382597E74E
GO

Sequence cautioni

The sequence AAD55648 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7 – 8Missing in AAM63155 (Ref. 5) Curated2
Sequence conflicti229A → V in AAM63155 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008017 Genomic DNA. Translation: AAD55648.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35403.1.
AK176548 mRNA. Translation: BAD44311.1.
BT030334 mRNA. Translation: ABO38747.1.
AY085944 mRNA. Translation: AAM63155.1.
PIRiE96755.
RefSeqiNP_565052.1. NM_105959.3.
UniGeneiAt.11680.

Genome annotation databases

EnsemblPlantsiAT1G73010.1; AT1G73010.1; AT1G73010.
GeneIDi843632.
GrameneiAT1G73010.1; AT1G73010.1; AT1G73010.
KEGGiath:AT1G73010.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPPSP1_ARATH
AccessioniPrimary (citable) accession number: Q67YC0
Secondary accession number(s): Q8LDK9, Q9SSM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: October 11, 2004
Last modified: April 12, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families