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Protein

Beta-glucosidase 8

Gene

BGLU8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421SubstrateBy similarity
Binding sitei139 – 1391SubstrateBy similarity
Binding sitei184 – 1841SubstrateBy similarity
Active sitei185 – 1851Proton donorBy similarity
Binding sitei319 – 3191SubstrateBy similarity
Active sitei387 – 3871NucleophileBy similarity
Binding sitei430 – 4301SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G62750-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 8 (EC:3.2.1.21)
Short name:
AtBGLU8
Gene namesi
Name:BGLU8
Ordered Locus Names:At3g62750
ORF Names:F26K9.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G62750.

Subcellular locationi

GO - Cellular componenti

  • vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 497475Beta-glucosidase 8PRO_0000389570Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi452 – 4521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi474 – 4741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ67XN2.
PRIDEiQ67XN2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G62750.1.

Structurei

3D structure databases

ProteinModelPortaliQ67XN2.
SMRiQ67XN2. Positions 24-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni437 – 4382Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ67XN2.
KOiK01188.
OMAiEATIFAF.
PhylomeDBiQ67XN2.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q67XN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHFNLLSII LVIVLATSYI DAFTRNDFPE DFLFGAGTSA YQWEGAANED
60 70 80 90 100
GRTPSVWDTT SHCYNGSNGD IACDGYHKYK EDVKLMAEMG LESFRFSISW
110 120 130 140 150
SRLIPNGRGR INPKGLLFYK NLIKELRSHG IEPHVTLYHY DLPQSLEDEY
160 170 180 190 200
GGWINHKIIE DFTAFADVCF REFGEDVKLW TTINEATIFA FAFYGKDVRY
210 220 230 240 250
GNCTTGNYCM ETYIAGHNML LAHASASNLY KLKYKSKQRG SIGLSIFALG
260 270 280 290 300
LTPYTNSKDD EIATQRAKAF LYGWMLKPLV FGDYPDEMKR TLGSRLPVFS
310 320 330 340 350
EEESEQVKGS SDFVGIIHYT TVYVTNQPAP YIFPSSTNKD FFTDMGAYII
360 370 380 390 400
STGNSSSFVF DAVPWGLEGV LQHIKHRYNN PPIYILENGS PMKHDSMLQD
410 420 430 440 450
TPRVEYIQAY IGAVLNAIKS GSDTRGYFVW SLIDLFEVQV GYKSSFGMYY
460 470 480 490
VNFSDPGRKR SPKLSASWYT GFLNGTIDVA SQDMTQLQRN FSGSSSL
Length:497
Mass (Da):56,178
Last modified:October 11, 2004 - v1
Checksum:i6FC878F48043BEAF
GO

Sequence cautioni

The sequence BAD44596.1 differs from that shown. Reason: Frameshift at position 194. Curated
The sequence CAB83125.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL162651 Genomic DNA. Translation: CAB83125.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE80387.1.
AK175256 mRNA. Translation: BAD43019.1.
AK176786 mRNA. Translation: BAD44549.1.
AK176833 mRNA. Translation: BAD44596.1. Frameshift.
PIRiT48064.
RefSeqiNP_191834.3. NM_116140.3.
UniGeneiAt.43830.

Genome annotation databases

EnsemblPlantsiAT3G62750.1; AT3G62750.1; AT3G62750.
GeneIDi825450.
KEGGiath:AT3G62750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL162651 Genomic DNA. Translation: CAB83125.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE80387.1.
AK175256 mRNA. Translation: BAD43019.1.
AK176786 mRNA. Translation: BAD44549.1.
AK176833 mRNA. Translation: BAD44596.1. Frameshift.
PIRiT48064.
RefSeqiNP_191834.3. NM_116140.3.
UniGeneiAt.43830.

3D structure databases

ProteinModelPortaliQ67XN2.
SMRiQ67XN2. Positions 24-468.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G62750.1.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ67XN2.
PRIDEiQ67XN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G62750.1; AT3G62750.1; AT3G62750.
GeneIDi825450.
KEGGiath:AT3G62750.

Organism-specific databases

TAIRiAT3G62750.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ67XN2.
KOiK01188.
OMAiEATIFAF.
PhylomeDBiQ67XN2.

Enzyme and pathway databases

BioCyciARA:AT3G62750-MONOMER.

Miscellaneous databases

PROiQ67XN2.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL08_ARATH
AccessioniPrimary (citable) accession number: Q67XN2
Secondary accession number(s): Q67XI6, Q9LZJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 11, 2004
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.