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Q67XN2 (BGL08_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 8

Short name=AtBGLU8
EC=3.2.1.21
Gene names
Name:BGLU8
Ordered Locus Names:At3g62750
ORF Names:F26K9.180
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence BAD44596.1 differs from that shown. Reason: Frameshift at position 194.

The sequence CAB83125.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentvacuole

Inferred from direct assay PubMed 15539469. Source: TAIR

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 497475Beta-glucosidase 8
PRO_0000389570

Regions

Region437 – 4382Substrate binding By similarity

Sites

Active site1851Proton donor By similarity
Active site3871Nucleophile By similarity
Binding site421Substrate By similarity
Binding site1391Substrate By similarity
Binding site1841Substrate By similarity
Binding site3191Substrate By similarity
Binding site4301Substrate By similarity

Amino acid modifications

Glycosylation651N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation3541N-linked (GlcNAc...) Potential
Glycosylation4521N-linked (GlcNAc...) Potential
Glycosylation4741N-linked (GlcNAc...) Potential
Glycosylation4901N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q67XN2 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 6FC878F48043BEAF

FASTA49756,178
        10         20         30         40         50         60 
MKHFNLLSII LVIVLATSYI DAFTRNDFPE DFLFGAGTSA YQWEGAANED GRTPSVWDTT 

        70         80         90        100        110        120 
SHCYNGSNGD IACDGYHKYK EDVKLMAEMG LESFRFSISW SRLIPNGRGR INPKGLLFYK 

       130        140        150        160        170        180 
NLIKELRSHG IEPHVTLYHY DLPQSLEDEY GGWINHKIIE DFTAFADVCF REFGEDVKLW 

       190        200        210        220        230        240 
TTINEATIFA FAFYGKDVRY GNCTTGNYCM ETYIAGHNML LAHASASNLY KLKYKSKQRG 

       250        260        270        280        290        300 
SIGLSIFALG LTPYTNSKDD EIATQRAKAF LYGWMLKPLV FGDYPDEMKR TLGSRLPVFS 

       310        320        330        340        350        360 
EEESEQVKGS SDFVGIIHYT TVYVTNQPAP YIFPSSTNKD FFTDMGAYII STGNSSSFVF 

       370        380        390        400        410        420 
DAVPWGLEGV LQHIKHRYNN PPIYILENGS PMKHDSMLQD TPRVEYIQAY IGAVLNAIKS 

       430        440        450        460        470        480 
GSDTRGYFVW SLIDLFEVQV GYKSSFGMYY VNFSDPGRKR SPKLSASWYT GFLNGTIDVA 

       490 
SQDMTQLQRN FSGSSSL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL162651 Genomic DNA. Translation: CAB83125.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE80387.1.
AK175256 mRNA. Translation: BAD43019.1.
AK176786 mRNA. Translation: BAD44549.1.
AK176833 mRNA. Translation: BAD44596.1. Frameshift.
PIRT48064.
RefSeqNP_191834.3. NM_116140.3.
UniGeneAt.43830.

3D structure databases

ProteinModelPortalQ67XN2.
SMRQ67XN2. Positions 24-468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G62750.1-P.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ67XN2.
PRIDEQ67XN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G62750.1; AT3G62750.1; AT3G62750.
GeneID825450.
KEGGath:AT3G62750.

Organism-specific databases

TAIRAT3G62750.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidQ67XN2.
OMAEATIFAF.
PhylomeDBQ67XN2.
ProtClustDBPLN02814.

Enzyme and pathway databases

BioCycARA:AT3G62750-MONOMER.

Gene expression databases

GenevestigatorQ67XN2.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL08_ARATH
AccessionPrimary (citable) accession number: Q67XN2
Secondary accession number(s): Q67XI6, Q9LZJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names