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Protein

Two-on-two hemoglobin-3

Gene

GLB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hemoglobin-like protein that exhibits an unusual concentration-independent binding of O2 and CO. May promote shoot organogenesis from root explants in vitro (PubMed:11526234, PubMed:21741261). Inhibits RGLG3 and RGLG4 ubiquitination activity (PubMed:27497447).3 Publications

Cofactori

heme1 PublicationNote: Binds 1 heme group per subunit.1 Publication

Absorptioni

Abs(max)=~411 nm1 PublicationThe absorbance spectrum of deoxy-GLB3 is unique as the protein forms a transient six-coordinate structure with absorption peaks at 538 and 565 nm after reduction and deoxygenation, which slowly converts to a five-coordinate structure with an absorption peak at 548 nm.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85HemeBy similarity1
Metal bindingi98Iron (heme proximal ligand)Combined sources1 Publication1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • oxygen binding Source: InterPro
  • oxygen transporter activity Source: TAIR

GO - Biological processi

  • oxygen transport Source: TAIR
  • response to auxin Source: TAIR
  • response to hypoxia Source: TAIR

Keywordsi

Biological processOxygen transport, Transport
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Two-on-two hemoglobin-3
Short name:
AtGLB3
Alternative name(s):
2-on-2 hemoglobin-3
Gene namesi
Name:GLB3
Ordered Locus Names:At4g32690
ORF Names:F4D11.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G32690.
TAIRilocus:2125662. AT4G32690.

Pathology & Biotechi

Disruption phenotypei

Slight reduction of the number of shoots produced by root explants in vitro; root explants are first cultured on an initial auxin-rich callus induction medium (CIM) followed by a transfer onto a cytokinin-containing shoot induction medium (SIM).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004255431 – 175Two-on-two hemoglobin-3Add BLAST175

Proteomic databases

PaxDbiQ67XG0.

Expressioni

Tissue specificityi

Expressed ubiquitously, with higher levels in root tissue than in shoot tissue.1 Publication

Gene expression databases

ExpressionAtlasiQ67XG0. baseline and differential.
GenevisibleiQ67XG0. AT.

Interactioni

Subunit structurei

Homodimer when ferric (Probable). Interacts with RGLG3 and RGLG4 (PubMed:27497447).Curated1 Publication

Protein-protein interaction databases

BioGridi14690. 3 interactors.
STRINGi3702.AT4G32690.1.

Structurei

Secondary structure

1175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 12Combined sources9
Helixi16 – 19Combined sources4
Helixi20 – 24Combined sources5
Helixi27 – 31Combined sources5
Helixi33 – 48Combined sources16
Helixi53 – 56Combined sources4
Helixi57 – 59Combined sources3
Helixi64 – 78Combined sources15
Helixi84 – 89Combined sources6
Helixi94 – 98Combined sources5
Helixi105 – 121Combined sources17
Helixi127 – 148Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C0NX-ray1.77A1-175[»]
4C44X-ray2.65A1-175[»]
ProteinModelPortaliQ67XG0.
SMRiQ67XG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IKRW. Eukaryota.
COG2346. LUCA.
HOGENOMiHOG000005818.
InParanoidiQ67XG0.
OMAiACKHAAN.
OrthoDBiEOG09360OBH.
PhylomeDBiQ67XG0.

Family and domain databases

InterProiView protein in InterPro
IPR009050. Globin-like.
IPR001486. Hemoglobin_trunc.
PfamiView protein in Pfam
PF01152. Bac_globin. 1 hit.
SUPFAMiSSF46458. SSF46458. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67XG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSLQDKASV LSGVDQAEAF AIDESNLFDK LGLQTFINLS TNFYTRVYDD
60 70 80 90 100
EEEWFQSIFS NSNKEDAIQN QYEFFVQRMG GPPLYSQRKG HPALIGRHRP
110 120 130 140 150
FPVTHQAAER WLEHMQNALD DSVDIDQDSK IKMMKFFRHT AFFLVAGNEL
160 170
KNQNEKPKHK PQCACKHAAN KPAEE
Length:175
Mass (Da):20,197
Last modified:October 11, 2004 - v1
Checksum:i7EE582552A3BBD7F
GO

Sequence cautioni

The sequence CAA18592 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79986 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167H → P in AAK55409 (PubMed:11526234).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF376062 mRNA. Translation: AAK55409.1.
AL022537 Genomic DNA. Translation: CAA18592.1. Sequence problems.
AL161581 Genomic DNA. Translation: CAB79986.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE86104.1.
AK175236 mRNA. Translation: BAD42999.1.
AK176859 mRNA. Translation: BAD44622.1.
AK220670 mRNA. Translation: BAD95217.1.
AK229442 mRNA. Translation: BAF01302.1.
BT024768 mRNA. Translation: ABD59106.1.
PIRiT04457.
RefSeqiNP_567901.1. NM_119421.5.
UniGeneiAt.20909.

Genome annotation databases

EnsemblPlantsiAT4G32690.1; AT4G32690.1; AT4G32690.
GeneIDi829404.
GrameneiAT4G32690.1; AT4G32690.1; AT4G32690.
KEGGiath:AT4G32690.

Similar proteinsi

Entry informationi

Entry nameiGLB3_ARATH
AccessioniPrimary (citable) accession number: Q67XG0
Secondary accession number(s): O65532, Q946U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: October 11, 2004
Last modified: May 10, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families