Q67XD9 (CINV2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline/neutral invertase CINV2
EC=3.2.1.26

Alternative name(s):
Cytosolic invertase 2

Gene names

Name:	CINV2
Ordered Locus Names:	At4g09510
ORF Names:	T15G18.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytosolic invertase that may cleave sucrose into glucose and fructose, and that is involved in the regulation of root growth. May regulate sugar-mediated root development by controlling sucrose catabolism in root cells. Ref.5
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Cytoplasm › cytosol.
Sequence similarities	Belongs to the glycosyl hydrolase 100 family.
Sequence caution	The sequence CAB78074.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	root development Inferred from genetic interaction Ref.5. Source: TAIR sucrose catabolic process Inferred from genetic interaction Ref.5. Source: TAIR
Cellular_component	cytosol Non-traceable author statement Ref.5. Source: TAIR
Molecular_function	beta-fructofuranosidase activity Inferred from genetic interaction Ref.5. Source: TAIR glycopeptide alpha-N-acetylgalactosaminidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q67XD9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q67XD9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     461-461: V → G
     462-558: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Alkaline/neutral invertase CINV2		PRO_0000422135
Natural variations
	Alternative sequence	461	1	V → G in isoform 2.		VSP_046440
	Alternative sequence	462 – 558	97	Missing in isoform 2.		VSP_046441

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 9472E6AEB1D3AB8A	FASTA	558	64,233
	10 20 30 40 50 60 MEEGHKEPLV LRVEGSHCSL SEMDDFDLTR ALEKPRQLKI ERKRSFDERS MSELSTGYVR 70 80 90 100 110 120 QDSILEMAHS PGSRSMVDTP LSVRNSFEPH PMVAEAWEAL RRSMVFFRGQ PVGTIAAYDH 130 140 150 160 170 180 ASEEVLNYDQ VFVRDFVPSA LAFLMNGEPD IVKNFLLKTL QLQGWEKRVD RFKLGEGVMP 190 200 210 220 230 240 ASFKVLHDPV RKTDTIIADF GESAIGRVAP VDSGFWWIIL LRAYTKSTGD LTLSETPECQ 250 260 270 280 290 300 RGMRLILSLC LSEGFDTFPT LLCADGCSMV DRRMGVYGYP IEIQALFFMA LRCALSMLKP 310 320 330 340 350 360 DEEGRDFIER IVKRLHALSF HMRSYFWLDF QQLNDIYRYK TEEYSHTAVN KFNVMPDSIP 370 380 390 400 410 420 DWVFDFMPLR GGYFVGNVSP ARMDFRWFSL GNCVSILSSL ATPDQSMAIM DLLEHRWEEL 430 440 450 460 470 480 VGEMPLKICY PCIESHEWRI VTGCDPKNTR WSYHNGGSWP VLLWTLTAAC IKTGRPQIAR 490 500 510 520 530 540 RAIDLIESRL HRDCWPEYYD GKQGRYVGKQ ARKYQTWSIA GYLVAKMMLE DPSHIGMISL 550 EEDKQMKPVI KRSASWTC
	Isoform 2 [UniParc]. Checksum: D09A81D8F986D417	FASTA	461	52,950
	10 20 30 40 50 60 MEEGHKEPLV LRVEGSHCSL SEMDDFDLTR ALEKPRQLKI ERKRSFDERS MSELSTGYVR 70 80 90 100 110 120 QDSILEMAHS PGSRSMVDTP LSVRNSFEPH PMVAEAWEAL RRSMVFFRGQ PVGTIAAYDH 130 140 150 160 170 180 ASEEVLNYDQ VFVRDFVPSA LAFLMNGEPD IVKNFLLKTL QLQGWEKRVD RFKLGEGVMP 190 200 210 220 230 240 ASFKVLHDPV RKTDTIIADF GESAIGRVAP VDSGFWWIIL LRAYTKSTGD LTLSETPECQ 250 260 270 280 290 300 RGMRLILSLC LSEGFDTFPT LLCADGCSMV DRRMGVYGYP IEIQALFFMA LRCALSMLKP 310 320 330 340 350 360 DEEGRDFIER IVKRLHALSF HMRSYFWLDF QQLNDIYRYK TEEYSHTAVN KFNVMPDSIP 370 380 390 400 410 420 DWVFDFMPLR GGYFVGNVSP ARMDFRWFSL GNCVSILSSL ATPDQSMAIM DLLEHRWEEL 430 440 450 460 VGEMPLKICY PCIESHEWRI VTGCDPKNTR WSYHNGGSWP G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase." Barratt D.H., Derbyshire P., Findlay K., Pike M., Wellner N., Lunn J., Feil R., Simpson C., Maule A.J., Smith A.M. Proc. Natl. Acad. Sci. U.S.A. 106:13124-13129(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL161515 Genomic DNA. Translation: CAB78074.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE82759.1. CP002687 Genomic DNA. Translation: AEE82760.1. AK176880 mRNA. Translation: BAD44643.1. BT028983 mRNA. Translation: ABI93892.1.
IPI	IPI00531622.
PIR	A85097.
RefSeq	NP_567347.1. NM_117019.4.
UniGene	At.33690.
3D structure databases
ProteinModelPortal	Q67XD9.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT4G09510.1-P.
Protein family/group databases
CAZy	GH100. Glycoside Hydrolase Family 100.
Proteomic databases
PaxDb	Q67XD9.
PRIDE	Q67XD9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G09510.1; AT4G09510.1; AT4G09510. AT4G09510.2; AT4G09510.2; AT4G09510.
GeneID	826535.
KEGG	ath:AT4G09510.
Organism-specific databases
TAIR	At4g09510.
Phylogenomic databases
eggNOG	NOG04872.
InParanoid	Q67XD9.
OMA	EMDDFDL.
PhylomeDB	Q67XD9.
ProtClustDB	PLN03005.
Enzyme and pathway databases
BioCyc	MetaCyc:AT4G09510-MONOMER.
Gene expression databases
ArrayExpress	Q67XD9.
Genevestigator	Q67XD9.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR024746. Glyco_hydro_100. [Graphical view]
Pfam	PF12899. Glyco_hydro_100. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CINV2_ARATH

Accession

Primary (citable) accession number: Q67XD9
Secondary accession number(s): Q08A96, Q9M0P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2013
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program