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Protein

Probable GDP-L-fucose synthase 1

Gene

Os06g0652400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.By similarity

Catalytic activityi

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH.By similarity

Pathwayi: GDP-L-fucose biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Putative GDP-L-fucose synthase 2 (Os06g0652300), Probable GDP-L-fucose synthase 1 (Os06g0652400)
This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose, the pathway GDP-L-fucose biosynthesis via de novo pathway and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei123Important for catalytic activityBy similarity1
Sitei125Important for catalytic activityBy similarity1
Active sitei152Proton donor/acceptorBy similarity1
Binding sitei156NADPBy similarity1
Sitei156Lowers pKa of active site TyrBy similarity1
Binding sitei195NADPBy similarity1
Binding sitei203SubstrateBy similarity1
Binding sitei218SubstrateBy similarity1
Binding sitei225SubstrateBy similarity1
Binding sitei285SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 31NADPBy similarity7
Nucleotide bindingi179 – 182NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Oxidoreductase
LigandNADP

Enzyme and pathway databases

ReactomeiR-OSA-6787639 GDP-fucose biosynthesis
UniPathwayiUPA00128; UER00191

Names & Taxonomyi

Protein namesi
Recommended name:
Probable GDP-L-fucose synthase 1 (EC:1.1.1.271By similarity)
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase 1
Gene namesi
Ordered Locus Names:Os06g0652400, LOC_Os06g44270
ORF Names:OsJ_22191Imported, OSJNBa0085J13.7
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743561 – 328Probable GDP-L-fucose synthase 1Add BLAST328

Proteomic databases

PaxDbiQ67WR2

Expressioni

Gene expression databases

ExpressionAtlasiQ67WR2 differential
GenevisibleiQ67WR2 OS

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os06g44270.1

Structurei

3D structure databases

ProteinModelPortaliQ67WR2
SMRiQ67WR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1431 Eukaryota
COG0451 LUCA
HOGENOMiHOG000168011
InParanoidiQ67WR2
KOiK02377
OMAiIHCAGRV
OrthoDBiEOG09360FC3

Family and domain databases

CDDicd05239 GDP_FS_SDR_e, 1 hit
HAMAPiMF_00956 GDP_fucose_synth, 1 hit
InterProiView protein in InterPro
IPR001509 Epimerase_deHydtase
IPR028614 GDP_fucose/colitose_synth
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01370 Epimerase, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Q67WR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTVTTADPH ASFLADKGGK VFVAGHRGLV GSAILRHLVS LGFTNVVVRT
60 70 80 90 100
HAELDLTRQS DVEAFFAAEL PRYVVLAAAK VGGIHANSTF PADFIAANLQ
110 120 130 140 150
IQTNVVDAAL KCGSVRKLLF LGSSCIYPKF APQPIPENSL LSGPLEPTNE
160 170 180 190 200
WYAVAKIAGI KMCQAYRIQH GFDAISAMPT NLYGPQDNFH PENSHVLPAL
210 220 230 240 250
IRRFHEAKAS NAAEVVVWGT GSPLREFLHV DDLADAVIFL MDHYSGLEHV
260 270 280 290 300
NVGSGSEVTI KELAELVKEV VGFQGKLVWD SSKPDGTPRK LMDSSKIQEM
310 320
GWKPKVPLKE GLVETYKWYV ENVISAKK
Length:328
Mass (Da):35,715
Last modified:October 11, 2004 - v1
Checksum:i87D1D0335D807119
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28G → S in AK071960 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003565 Genomic DNA Translation: BAD37407.1
AP008212 Genomic DNA Translation: BAF20149.1
AP014962 Genomic DNA Translation: BAS98916.1
CM000143 Genomic DNA Translation: EAZ37847.1
AK071960 mRNA No translation available.
AK103529 mRNA Translation: BAG96133.1
AK120997 mRNA Translation: BAH00264.1
RefSeqiXP_015644414.1, XM_015788928.1
UniGeneiOs.53321

Genome annotation databases

EnsemblPlantsiOs06t0652400-01; Os06t0652400-01; Os06g0652400
Os06t0652400-02; Os06t0652400-02; Os06g0652400
GeneIDi4341699
GrameneiOs06t0652400-01; Os06t0652400-01; Os06g0652400
Os06t0652400-02; Os06t0652400-02; Os06g0652400
KEGGiosa:4341699

Similar proteinsi

Entry informationi

Entry nameiFCL1_ORYSJ
AccessioniPrimary (citable) accession number: Q67WR2
Secondary accession number(s): Q0DAH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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