Reviewed, UniProtKB/Swiss-Prot Q67W82 (4CL4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable 4-coumarate--CoA ligase 4
      Short name=4CL 4
      Short name=Os4CL4
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 4

Gene names

Name:	4CL4
Ordered Locus Names:	Os06g0656500, LOC_Os06g44620
ORF Names:	P0460H04.31-1, P0460H04.31-2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q67W82-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q67W82-2)

The sequence of this isoform differs from the canonical sequence as follows:
     408-445: YLNDPESTKN...DDEIFIVDRL → SISLSFFPLH...YGHASACTTT
     446-559: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Probable 4-coumarate--CoA ligase 4		PRO_0000351625
Regions
	Nucleotide binding	198 – 206	9	ATP By similarity
	Nucleotide binding	341 – 346	6	ATP By similarity
	Region	271 – 340	70	SBD1
	Region	341 – 408	68	SBD2
Sites
	Binding site	429	1	ATP By similarity
	Binding site	444	1	ATP By similarity
	Binding site	535	1	ATP By similarity
Natural variations
	Alternative sequence	408 – 445	38	YLNDP…IVDRL → SISLSFFPLHIIIGIISAKS IIHHLPRVYGHASACTTT in isoform 2.		VSP_035525
	Alternative sequence	446 – 559	114	Missing in isoform 2.		VSP_035526

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: DAC957A75C3E45BA	FASTA	559	60,029
	10 20 30 40 50 60 MGSMAAAAEA AQEEETVVFR SKLPDIEIPS HLTLQAYCFE KLPEVAARPC LIDGQTGAVY 70 80 90 100 110 120 SYGEVEELSR RAAAGLRRLG VGKGDVVMSL LRNCPEFAFT FLGAARLGAA TTTANPFYTP 130 140 150 160 170 180 HEIHRQASAA GARVIVTEAC AVEKVRGFAA DRGIPVVAVD GDFDGCVGFG EAMLDASIEP 190 200 210 220 230 240 LDADEEVHPD DVVALPYSSG TTGLPKGVML THRSLVTSVA QQVDGENPNL YFRREDVVLC 250 260 270 280 290 300 LLPLFHIYSL NSVLLAGLRA GSAIVIMRKF DLGALVDLTR RHGVTVAPFV PPIVVEIAKS 310 320 330 340 350 360 PRVTADDLAS IRMVMSGAAP MGKDLQDAFM AKIPNAVLGQ GYGMTEAGPV LAMCLAFAKE 370 380 390 400 410 420 PFEVKSGSCG TVVRNAELKI VDPDTGATLG RNQSGEICIR GEQIMKGYLN DPESTKNTID 430 440 450 460 470 480 KGGWLHTGDI GYVDDDDEIF IVDRLKEIIK YKGFQVPPAE LEALLITHPD IKDAAVVPMI 490 500 510 520 530 540 DEIAGEVPVA FIVRIEGSAI SENEIKQFVA KEVVFYKRLN KVFFADSIPK SPSGKILRKD 550 LRAKLAAGIP TNDNTQLKS
	Isoform 2. Checksum: 546A33B5B6DBDFBA	FASTA	445	47,220
	10 20 30 40 50 60 MGSMAAAAEA AQEEETVVFR SKLPDIEIPS HLTLQAYCFE KLPEVAARPC LIDGQTGAVY 70 80 90 100 110 120 SYGEVEELSR RAAAGLRRLG VGKGDVVMSL LRNCPEFAFT FLGAARLGAA TTTANPFYTP 130 140 150 160 170 180 HEIHRQASAA GARVIVTEAC AVEKVRGFAA DRGIPVVAVD GDFDGCVGFG EAMLDASIEP 190 200 210 220 230 240 LDADEEVHPD DVVALPYSSG TTGLPKGVML THRSLVTSVA QQVDGENPNL YFRREDVVLC 250 260 270 280 290 300 LLPLFHIYSL NSVLLAGLRA GSAIVIMRKF DLGALVDLTR RHGVTVAPFV PPIVVEIAKS 310 320 330 340 350 360 PRVTADDLAS IRMVMSGAAP MGKDLQDAFM AKIPNAVLGQ GYGMTEAGPV LAMCLAFAKE 370 380 390 400 410 420 PFEVKSGSCG TVVRNAELKI VDPDTGATLG RNQSGEICIR GEQIMKGSIS LSFFPLHIII 430 440 GIISAKSIIH HLPRVYGHAS ACTTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.
[4]	"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella." de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J. New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003712 Genomic DNA. Translation: BAD37587.1. AP003712 Genomic DNA. Translation: BAD37588.1. AP008212 Genomic DNA. Translation: BAF20166.1. AK067261 mRNA. No translation available. AK068985 mRNA. No translation available.
RefSeq	NP_001058252.1.
UniGene	Os.8020
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4341718.
KEGG	osa:4341718.
Organism-specific databases
Gramene	Q67W82.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL4_ORYSJ

Accession

Primary (citable) accession number: Q67W82
Secondary accession number(s): Q67W81

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 11, 2004
Last modified:	March 24, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)