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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

Os06g0236900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei156S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei159UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Ordered Locus Names:Os06g0236900, LOC_Os06g12990
ORF Names:OsJ_20740, OSJNBa0068B06.2, OSJNBa0080E19.41
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705821 – 259tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST259

Proteomic databases

PaxDbiQ67V78.

Expressioni

Gene expression databases

GenevisibleiQ67V78. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g12990.1.

Structurei

3D structure databases

ProteinModelPortaliQ67V78.
SMRiQ67V78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 104S-adenosyl-L-methionine bindingUniRule annotation2
Regioni136 – 137S-adenosyl-L-methionine bindingUniRule annotation2
Regioni234 – 236S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
InParanoidiQ67V78.
KOiK03439.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF16. PTHR23417:SF16. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67V78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGDGANGG GGGGQGKLPR KRFYRARAHS NPLSDSHFPI PISPDEVDLS
60 70 80 90 100
QHYPRYFPSG EGEARQGDAA VPRIRFADVG CGFGGLLVGL STLFPDTLMI
110 120 130 140 150
GMELRDKVTE YVKERILALR ASNPGKYDNI SVVRTNSMKY IPNYFRKAQL
160 170 180 190 200
SKMFFLFPDP HFKEKNHRRR VISMQLLDEY AYVMEVGGII YTITDVEELG
210 220 230 240 250
EWMRSCLEKH PLFEAIPEEE TKADPVVKLL STATEEGQKV ARNGGQTFQA

IFRRISLQE
Length:259
Mass (Da):29,078
Last modified:October 11, 2004 - v1
Checksum:i378AC5DAA0F9AAF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004995 Genomic DNA. Translation: BAD37888.1.
AP005170 Genomic DNA. Translation: BAD37941.1.
AP014962 Genomic DNA. No translation available.
CM000143 Genomic DNA. Translation: EAZ36410.1.
RefSeqiXP_015643817.1. XM_015788331.1.
UniGeneiOs.13757.

Genome annotation databases

GeneIDi4340601.
KEGGiosa:4340601.

Similar proteinsi

Entry informationi

Entry nameiTRMB_ORYSJ
AccessioniPrimary (citable) accession number: Q67V78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 11, 2004
Last modified: October 25, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families