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Q67V78

- TRMB_ORYSJ

UniProt

Q67V78 - TRMB_ORYSJ

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

Os06g0236900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei156 – 1561S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei159 – 1591UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. nucleotide biosynthetic process Source: EnsemblPlants/Gramene
  2. protein targeting to mitochondrion Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Ordered Locus Names:Os06g0236900, LOC_Os06g12990
ORF Names:OsJ_20740, OSJNBa0068B06.2, OSJNBa0080E19.41
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ67V78.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259tRNA (guanine-N(7)-)-methyltransferasePRO_0000370582Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiQ67V78. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ67V78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 1042S-adenosyl-L-methionine bindingUniRule annotation
Regioni136 – 1372S-adenosyl-L-methionine bindingUniRule annotation
Regioni234 – 2363S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiQ67V78.
OMAiRAHSNPI.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q67V78-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASGDGANGG GGGGQGKLPR KRFYRARAHS NPLSDSHFPI PISPDEVDLS
60 70 80 90 100
QHYPRYFPSG EGEARQGDAA VPRIRFADVG CGFGGLLVGL STLFPDTLMI
110 120 130 140 150
GMELRDKVTE YVKERILALR ASNPGKYDNI SVVRTNSMKY IPNYFRKAQL
160 170 180 190 200
SKMFFLFPDP HFKEKNHRRR VISMQLLDEY AYVMEVGGII YTITDVEELG
210 220 230 240 250
EWMRSCLEKH PLFEAIPEEE TKADPVVKLL STATEEGQKV ARNGGQTFQA

IFRRISLQE
Length:259
Mass (Da):29,078
Last modified:October 11, 2004 - v1
Checksum:i378AC5DAA0F9AAF9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP004995 Genomic DNA. Translation: BAD37888.1.
AP005170 Genomic DNA. Translation: BAD37941.1.
CM000143 Genomic DNA. Translation: EAZ36410.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP004995 Genomic DNA. Translation: BAD37888.1 .
AP005170 Genomic DNA. Translation: BAD37941.1 .
CM000143 Genomic DNA. Translation: EAZ36410.1 .

3D structure databases

ProteinModelPortali Q67V78.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei Q67V78.

Phylogenomic databases

eggNOGi COG0220.
InParanoidi Q67V78.
OMAi RAHSNPI.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Gene expression databases

ExpressionAtlasi Q67V78. baseline.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiTRMB_ORYSJ
AccessioniPrimary (citable) accession number: Q67V78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3