Reviewed, UniProtKB/Swiss-Prot Q67UW5 (GUN5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 5
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 5

Gene names

Ordered Locus Names:	Os02g0151300, LOC_Os02g05744
ORF Names:	OSJNBa0050G13.22

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence AK120536 differs from that shown. Reason: Erroneous termination at position 365. Translated as Tyr. The sequence AK120536 differs from that shown. Reason: Frameshift at positions 351 and 357.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 534	507	Endoglucanase 5		PRO_0000249282
Sites
	Active site	432	1	By similarity
	Active site	484	1	By similarity
	Active site	493	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q67UW5-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 9BA71564266869E2	FASTA	534	58,556
	10 20 30 40 50 60 MSDVSGRFVV AAAVVAVSLA MAAAAAAHDY GEALSKSLLY FEAQRSGRLP YNQRVRWRGH 70 80 90 100 110 120 SGLTDGLEQG VDLVGGYYDA GDHVKFGLPM AFTVTMLSWS VLEYGEEIAA AGELGHALHA 130 140 150 160 170 180 IKWGTDYFIK AHTHPNVLWT QVGDGDSDHY CWQRPEDMTT SRHAYKVDAE NPGSEVAAET 190 200 210 220 230 240 AAAMAAASIV FRRAGDAHYA HLLLHHAQQL FEFGDKYRGR YDESVEVVKN YYPSSSGYKD 250 260 270 280 290 300 ELLWAALWLH RATGRREYLD YAVDNADDFG GTGWAVSEFS WDIKYAGLQV LASKLLVEEK 310 320 330 340 350 360 HLSSQQREVL EKYRSKAEYY VCSCMGRNPG GAAHNAGRTP AGLLFIRPWN NLQYVSNAAF 370 380 390 400 410 420 LLTVYSDVLS YLSLPLLCPD PDAAADEAAP AAADAGEVLE FARSQADYIL GTNPMATSYL 430 440 450 460 470 480 VGYGEAYPRR VHHRAASSAS YARDRDFIGC LQGFDSWYSA AAENPHDLVG AVVGGPNGND 490 500 510 520 530 VFTDHRGAYM QTEACTYNTA PMVGVFSRLM ELERRRRGED APPSSTSPVA EDDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP005412 Genomic DNA. Translation: BAD38054.1. AP008208 Genomic DNA. Translation: BAF07821.1. AK120536 mRNA. No translation available.
RefSeq	NP_001045907.1.
UniGene	Os.10513
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4328316.
KEGG	osa:4328316.
Organism-specific databases
Gramene	Q67UW5.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN5_ORYSJ

Accession

Primary (citable) accession number: Q67UW5
Secondary accession number(s): Q0E3W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)