Reviewed, UniProtKB/Swiss-Prot Q67U69 (FDH2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase 2, mitochondrial
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase 2
      Short name=FDH 2

Gene names

Ordered Locus Names:	Os06g0486900, LOC_Os06g29220
ORF Names:	P0404G03.13

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 18	18	Mitochondrion Potential
	Chain	19 – 378	360	Formate dehydrogenase 2, mitochondrial		PRO_0000225339
Sites
	Active site	284	1	By similarity
	Active site	332	1	Proton donor By similarity
Experimental info
	Sequence conflict	41	1	E → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q67U69-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 7DFE10FAABC0E2EF	FASTA	378	41,267
	10 20 30 40 50 60 MAMWRAPSAA GQLLGRALAS TAAQTSAGSK KVVGVFYKGG EYADKNPNFV GCVDSALGIR 70 80 90 100 110 120 GWLESKGHRY IVTDDKEGIN CELEKHIEDA HVLITTPFHP AYITAERIKK AKNLELLLTA 130 140 150 160 170 180 GVGSDHIDLP AAAAAGLTVA EITGSNTVSV AEDQLMRILL LLRNFLPGHH QIVNGEWNVA 190 200 210 220 230 240 GIAHRTYDLE GKTVGTVGAG RIGRLLLQRL KPFNCNLMYH DRVKIDPELE KEIGAKYEED 250 260 270 280 290 300 LDAMLPKCDV VVINMPLTEK TRGMFNKERI AKMKKGVTIV NNARGAIMDT QAVADACASG 310 320 330 340 350 360 HVAGYGGDVW FPQPAPKDHP WRYMPNHAMT PHCSGTTIDG QLRYAAGVKD MLDRYFKGED 370 FPAQNYIVKA GQLASQYQ

References

[1]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP005656 Genomic DNA. Translation: BAD38302.1. AK064610 mRNA. No translation available.
RefSeq	NP_001057667.1.
UniGene	Os.14187
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4341070.
KEGG	osa:4341070.
Organism-specific databases
Gramene	Q67U69.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH2_ORYSJ

Accession

Primary (citable) accession number: Q67U69

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)