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Protein

Formate dehydrogenase 2, mitochondrial

Gene

Os06g0486900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD+-dependent oxidation of formate to carbon dioxide. Involved in the cell stress response.UniRule annotation

Catalytic activityi

Formate + NAD+ = CO2 + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122Substrate; via amide nitrogenUniRule annotation1
Binding sitei146SubstrateUniRule annotation1
Binding sitei147NADUniRule annotation1
Binding sitei221NADUniRule annotation1
Binding sitei282NAD; via carbonyl oxygenUniRule annotation1
Sitei284Important for catalytic activityUniRule annotation1
Binding sitei308NADUniRule annotation1
Sitei332Important for catalytic activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi201 – 202NADUniRule annotation2
Nucleotide bindingi256 – 260NADUniRule annotation5
Nucleotide bindingi332 – 335NADUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenase 2, mitochondrialUniRule annotation (EC:1.2.1.2UniRule annotation)
Short name:
FDH 2UniRule annotation
Alternative name(s):
NAD-dependent formate dehydrogenase 2UniRule annotation
Gene namesi
Ordered Locus Names:Os06g0486900, LOC_Os06g29220
ORF Names:OsJ_21395Imported, P0404G03.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 18MitochondrionUniRule annotationAdd BLAST18
ChainiPRO_000022533919 – 378Formate dehydrogenase 2, mitochondrialAdd BLAST360

Proteomic databases

PaxDbiQ67U69.

Expressioni

Gene expression databases

GenevisibleiQ67U69. OS.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi39947.LOC_Os06g29220.1.

Structurei

3D structure databases

ProteinModelPortaliQ67U69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 146CatalyticUniRule annotationAdd BLAST116
Regioni147 – 333Coenzyme-bindingUniRule annotationAdd BLAST187
Regioni334 – 377CatalyticUniRule annotationAdd BLAST44

Sequence similaritiesi

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0069. Eukaryota.
COG1052. LUCA.
HOGENOMiHOG000136703.
InParanoidiQ67U69.
KOiK00122.
OMAiELIYYDY.
OrthoDBiEOG09360BWJ.

Family and domain databases

CDDicd05302. FDH. 1 hit.
Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase. 1 hit.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR033689. FDH_NAD-dep.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q67U69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMWRAPSAA GQLLGRALAS TAAQTSAGSK KVVGVFYKGG EYADKNPNFV
60 70 80 90 100
GCVDSALGIR GWLESKGHRY IVTDDKEGIN CELEKHIEDA HVLITTPFHP
110 120 130 140 150
AYITAERIKK AKNLELLLTA GVGSDHIDLP AAAAAGLTVA EITGSNTVSV
160 170 180 190 200
AEDQLMRILL LLRNFLPGHH QIVNGEWNVA GIAHRTYDLE GKTVGTVGAG
210 220 230 240 250
RIGRLLLQRL KPFNCNLMYH DRVKIDPELE KEIGAKYEED LDAMLPKCDV
260 270 280 290 300
VVINMPLTEK TRGMFNKERI AKMKKGVTIV NNARGAIMDT QAVADACASG
310 320 330 340 350
HVAGYGGDVW FPQPAPKDHP WRYMPNHAMT PHCSGTTIDG QLRYAAGVKD
360 370
MLDRYFKGED FPAQNYIVKA GQLASQYQ
Length:378
Mass (Da):41,267
Last modified:October 11, 2004 - v1
Checksum:i7DFE10FAABC0E2EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41E → K in AK064610 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005656 Genomic DNA. Translation: BAD38302.1.
AP008212 Genomic DNA. Translation: BAF19581.1.
AP014962 Genomic DNA. Translation: BAS97837.1.
CM000143 Genomic DNA. Translation: EAZ37052.1.
AK064610 mRNA. No translation available.
RefSeqiXP_015642621.1. XM_015787135.1.
UniGeneiOs.14187.

Genome annotation databases

EnsemblPlantsiOS06T0486900-01; OS06T0486900-01; OS06G0486900.
GeneIDi4341070.
GrameneiOS06T0486900-01; OS06T0486900-01; OS06G0486900.
KEGGiosa:4341070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005656 Genomic DNA. Translation: BAD38302.1.
AP008212 Genomic DNA. Translation: BAF19581.1.
AP014962 Genomic DNA. Translation: BAS97837.1.
CM000143 Genomic DNA. Translation: EAZ37052.1.
AK064610 mRNA. No translation available.
RefSeqiXP_015642621.1. XM_015787135.1.
UniGeneiOs.14187.

3D structure databases

ProteinModelPortaliQ67U69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g29220.1.

Proteomic databases

PaxDbiQ67U69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0486900-01; OS06T0486900-01; OS06G0486900.
GeneIDi4341070.
GrameneiOS06T0486900-01; OS06T0486900-01; OS06G0486900.
KEGGiosa:4341070.

Phylogenomic databases

eggNOGiKOG0069. Eukaryota.
COG1052. LUCA.
HOGENOMiHOG000136703.
InParanoidiQ67U69.
KOiK00122.
OMAiELIYYDY.
OrthoDBiEOG09360BWJ.

Gene expression databases

GenevisibleiQ67U69. OS.

Family and domain databases

CDDicd05302. FDH. 1 hit.
Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase. 1 hit.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR033689. FDH_NAD-dep.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFDH2_ORYSJ
AccessioniPrimary (citable) accession number: Q67U69
Secondary accession number(s): Q0DC42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.