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Protein

Thiamine biosynthesis bifunctional protein ThiM/ThiE

Gene

thiM/thiE

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.By similarity
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2.By similarity
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2.By similarity
4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine biosynthesis bifunctional protein ThiM/ThiE (thiM/thiE)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine biosynthesis bifunctional protein ThiM/ThiE (thiM/thiE)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei404-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity1
Binding sitei116ATPBy similarity1
Binding sitei164ATPBy similarity1
Binding sitei1914-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity1
Binding sitei335HMP-PPBy similarity1
Metal bindingi336MagnesiumBy similarity1
Metal bindingi355MagnesiumBy similarity1
Binding sitei374HMP-PPBy similarity1
Binding sitei403HMP-PPBy similarity1
Binding sitei431THZ-P; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis bifunctional protein ThiM/ThiE
Including the following 2 domains:
Hydroxyethylthiazole kinase (EC:2.7.1.50By similarity)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
Thz kinase
Thiamine-phosphate synthase (EC:2.5.1.3By similarity)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Gene namesi
Name:thiM/thiE
Ordered Locus Names:STH157
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSymbiobacteriaceaeSymbiobacterium
Proteomesi
  • UP000000417 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003839121 – 480Thiamine biosynthesis bifunctional protein ThiM/ThiEAdd BLAST480

Interactioni

Protein-protein interaction databases

STRINGi292459.STH157.

Structurei

3D structure databases

ProteinModelPortaliQ67T51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 287Hydroxyethylthiazole kinaseAdd BLAST287
Regioni288 – 480Thiamine-phosphate synthaseAdd BLAST193
Regioni303 – 307HMP-PP bindingBy similarity5
Regioni400 – 402THZ-P bindingBy similarity3
Regioni451 – 452THZ-P bindingBy similarity2

Sequence similaritiesi

In the N-terminal section; belongs to the Thz kinase family.Curated
In the C-terminal section; belongs to the thiamine-phosphate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG0352. LUCA.
COG2145. LUCA.
HOGENOMiHOG000097679.
OMAiALMCAED.
OrthoDBiPOG091H004S.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
cd00564. TMP_TenI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
MF_00097. TMP_synthase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67T51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLPERVRE RRPLVHAITN CVTMEWVARG LLAAGARPVM ARDAAEAPVV
60 70 80 90 100
AAAADALVLN LGTWSPGLQQ AMLEAGQVAA RRGIPVVLDP VGAGGTETRT
110 120 130 140 150
RAALELLERV RVTAVRGNAG EILALAGRDG LVRGVDGPDG RPGPQTERAA
160 170 180 190 200
RAVARRFGCL VAVTGATDLV TDGRRTLAVR AGHPLMSQVP GTGCLATALV
210 220 230 240 250
AAALAAGTGA GPAGRDRPME DVDVVAEALL WAGWAGEQAA SAASGPGTFA
260 270 280 290 300
AAFLDRLALR GPLPPGRIAP PLSERLSLYV LVSGATPPDV LEAVLQAGCR
310 320 330 340 350
MIQFREKRLP LPAQLEAAAR VREACRRHGA LLVVNDRVDL ALAVGADGVH
360 370 380 390 400
LGQEDLPVAA ARRILGPDAV IGATCETAGE ARAARDAGAD YIGAGPVYVT
410 420 430 440 450
PSKPDAGEPY GPDVVRRVSE AADLPVVGIG GIGPGRAAPV IAAGAAGVAV
460 470 480
ISAVLGAPDP GAAARAILDE VRRAKGEVSA
Length:480
Mass (Da):48,683
Last modified:October 11, 2004 - v1
Checksum:i0FEC441B02894F29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD39142.1.

Genome annotation databases

EnsemblBacteriaiBAD39142; BAD39142; STH157.
KEGGisth:STH157.
PATRICi23778373. VBISymThe116959_0158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD39142.1.

3D structure databases

ProteinModelPortaliQ67T51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292459.STH157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD39142; BAD39142; STH157.
KEGGisth:STH157.
PATRICi23778373. VBISymThe116959_0158.

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG0352. LUCA.
COG2145. LUCA.
HOGENOMiHOG000097679.
OMAiALMCAED.
OrthoDBiPOG091H004S.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
cd00564. TMP_TenI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
MF_00097. TMP_synthase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIME_SYMTH
AccessioniPrimary (citable) accession number: Q67T51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.