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Q67T51

- THIME_SYMTH

UniProt

Q67T51 - THIME_SYMTH

Protein

Thiamine biosynthesis bifunctional protein ThiM/ThiE

Gene

thiM/thiE

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
    2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 4014-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity
    Binding sitei116 – 1161ATPBy similarity
    Binding sitei164 – 1641ATPBy similarity
    Binding sitei191 – 19114-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity
    Binding sitei335 – 3351HMP-PPBy similarity
    Metal bindingi336 – 3361MagnesiumBy similarity
    Metal bindingi355 – 3551MagnesiumBy similarity
    Binding sitei374 – 3741HMP-PPBy similarity
    Binding sitei403 – 4031HMP-PPBy similarity
    Binding sitei431 – 4311THZ-P; via amide nitrogenBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSTHE292459:GJMM-173-MONOMER.
    UniPathwayiUPA00060; UER00139.
    UPA00060; UER00141.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine biosynthesis bifunctional protein ThiM/ThiE
    Including the following 2 domains:
    Hydroxyethylthiazole kinase (EC:2.7.1.50)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
    Short name:
    TH kinase
    Short name:
    Thz kinase
    Thiamine-phosphate synthase (EC:2.5.1.3)
    Short name:
    TMP-PPase
    Short name:
    TP synthase
    Short name:
    TPS
    Alternative name(s):
    Thiamine-phosphate pyrophosphorylase
    Short name:
    TMP pyrophosphorylase
    Gene namesi
    Name:thiM/thiE
    Ordered Locus Names:STH157
    OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
    Taxonomic identifieri292459 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium
    ProteomesiUP000000417: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 480480Thiamine biosynthesis bifunctional protein ThiM/ThiEPRO_0000383912Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi292459.STH157.

    Structurei

    3D structure databases

    ProteinModelPortaliQ67T51.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 287287Hydroxyethylthiazole kinaseAdd
    BLAST
    Regioni288 – 480193Thiamine-phosphate synthaseAdd
    BLAST
    Regioni303 – 3075HMP-PP bindingBy similarity
    Regioni400 – 4023THZ-P bindingBy similarity
    Regioni451 – 4522THZ-P bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the Thz kinase family.Curated
    In the C-terminal section; belongs to the thiamine-phosphate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0352.
    HOGENOMiHOG000097679.
    OMAiRECKEND.
    OrthoDBiEOG628F8M.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.1190.20. 1 hit.
    HAMAPiMF_00097. TMP_synthase.
    MF_00228. Thz_kinase.
    InterProiIPR013785. Aldolase_TIM.
    IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    IPR022998. ThiaminP_synth_SF.
    IPR003733. TMP_synthase.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    PF02581. TMP-TENI. 1 hit.
    [Graphical view]
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF51391. SSF51391. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00693. thiE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q67T51-1 [UniParc]FASTAAdd to Basket

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    MSTLPERVRE RRPLVHAITN CVTMEWVARG LLAAGARPVM ARDAAEAPVV    50
    AAAADALVLN LGTWSPGLQQ AMLEAGQVAA RRGIPVVLDP VGAGGTETRT 100
    RAALELLERV RVTAVRGNAG EILALAGRDG LVRGVDGPDG RPGPQTERAA 150
    RAVARRFGCL VAVTGATDLV TDGRRTLAVR AGHPLMSQVP GTGCLATALV 200
    AAALAAGTGA GPAGRDRPME DVDVVAEALL WAGWAGEQAA SAASGPGTFA 250
    AAFLDRLALR GPLPPGRIAP PLSERLSLYV LVSGATPPDV LEAVLQAGCR 300
    MIQFREKRLP LPAQLEAAAR VREACRRHGA LLVVNDRVDL ALAVGADGVH 350
    LGQEDLPVAA ARRILGPDAV IGATCETAGE ARAARDAGAD YIGAGPVYVT 400
    PSKPDAGEPY GPDVVRRVSE AADLPVVGIG GIGPGRAAPV IAAGAAGVAV 450
    ISAVLGAPDP GAAARAILDE VRRAKGEVSA 480
    Length:480
    Mass (Da):48,683
    Last modified:October 11, 2004 - v1
    Checksum:i0FEC441B02894F29
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006840 Genomic DNA. Translation: BAD39142.1.
    RefSeqiYP_073986.1. NC_006177.1.

    Genome annotation databases

    EnsemblBacteriaiBAD39142; BAD39142; STH157.
    GeneIDi2981020.
    KEGGisth:STH157.
    PATRICi23778373. VBISymThe116959_0158.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006840 Genomic DNA. Translation: BAD39142.1 .
    RefSeqi YP_073986.1. NC_006177.1.

    3D structure databases

    ProteinModelPortali Q67T51.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 292459.STH157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD39142 ; BAD39142 ; STH157 .
    GeneIDi 2981020.
    KEGGi sth:STH157.
    PATRICi 23778373. VBISymThe116959_0158.

    Phylogenomic databases

    eggNOGi COG0352.
    HOGENOMi HOG000097679.
    OMAi RECKEND.
    OrthoDBi EOG628F8M.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    UPA00060 ; UER00141 .
    BioCyci STHE292459:GJMM-173-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.1190.20. 1 hit.
    HAMAPi MF_00097. TMP_synthase.
    MF_00228. Thz_kinase.
    InterProi IPR013785. Aldolase_TIM.
    IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    IPR022998. ThiaminP_synth_SF.
    IPR003733. TMP_synthase.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    PF02581. TMP-TENI. 1 hit.
    [Graphical view ]
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF51391. SSF51391. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00693. thiE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
      Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
      Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: T / IAM 14863.

    Entry informationi

    Entry nameiTHIME_SYMTH
    AccessioniPrimary (citable) accession number: Q67T51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3