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Protein

Thiamine biosynthesis bifunctional protein ThiM/ThiE

Gene

thiM/thiE

Organism
Symbiobacterium thermophilum (strain T / IAM 14863)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 4014-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity
Binding sitei116 – 1161ATPBy similarity
Binding sitei164 – 1641ATPBy similarity
Binding sitei191 – 19114-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity
Binding sitei335 – 3351HMP-PPBy similarity
Metal bindingi336 – 3361MagnesiumBy similarity
Metal bindingi355 – 3551MagnesiumBy similarity
Binding sitei374 – 3741HMP-PPBy similarity
Binding sitei403 – 4031HMP-PPBy similarity
Binding sitei431 – 4311THZ-P; via amide nitrogenBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE292459:GJMM-173-MONOMER.
UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis bifunctional protein ThiM/ThiE
Including the following 2 domains:
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
Thz kinase
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TMP-PPase
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Gene namesi
Name:thiM/thiE
Ordered Locus Names:STH157
OrganismiSymbiobacterium thermophilum (strain T / IAM 14863)
Taxonomic identifieri292459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSymbiobacteriaceaeSymbiobacterium
ProteomesiUP000000417: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Thiamine biosynthesis bifunctional protein ThiM/ThiEPRO_0000383912Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi292459.STH157.

Structurei

3D structure databases

ProteinModelPortaliQ67T51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 287287Hydroxyethylthiazole kinaseAdd
BLAST
Regioni288 – 480193Thiamine-phosphate synthaseAdd
BLAST
Regioni303 – 3075HMP-PP bindingBy similarity
Regioni400 – 4023THZ-P bindingBy similarity
Regioni451 – 4522THZ-P bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the Thz kinase family.Curated
In the C-terminal section; belongs to the thiamine-phosphate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0352.
HOGENOMiHOG000097679.
OMAiGANPERP.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
MF_00228. Thz_kinase.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q67T51-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTLPERVRE RRPLVHAITN CVTMEWVARG LLAAGARPVM ARDAAEAPVV
60 70 80 90 100
AAAADALVLN LGTWSPGLQQ AMLEAGQVAA RRGIPVVLDP VGAGGTETRT
110 120 130 140 150
RAALELLERV RVTAVRGNAG EILALAGRDG LVRGVDGPDG RPGPQTERAA
160 170 180 190 200
RAVARRFGCL VAVTGATDLV TDGRRTLAVR AGHPLMSQVP GTGCLATALV
210 220 230 240 250
AAALAAGTGA GPAGRDRPME DVDVVAEALL WAGWAGEQAA SAASGPGTFA
260 270 280 290 300
AAFLDRLALR GPLPPGRIAP PLSERLSLYV LVSGATPPDV LEAVLQAGCR
310 320 330 340 350
MIQFREKRLP LPAQLEAAAR VREACRRHGA LLVVNDRVDL ALAVGADGVH
360 370 380 390 400
LGQEDLPVAA ARRILGPDAV IGATCETAGE ARAARDAGAD YIGAGPVYVT
410 420 430 440 450
PSKPDAGEPY GPDVVRRVSE AADLPVVGIG GIGPGRAAPV IAAGAAGVAV
460 470 480
ISAVLGAPDP GAAARAILDE VRRAKGEVSA
Length:480
Mass (Da):48,683
Last modified:October 11, 2004 - v1
Checksum:i0FEC441B02894F29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD39142.1.
RefSeqiWP_011194292.1. NC_006177.1.
YP_073986.1. NC_006177.1.

Genome annotation databases

EnsemblBacteriaiBAD39142; BAD39142; STH157.
GeneIDi2981020.
KEGGisth:STH157.
PATRICi23778373. VBISymThe116959_0158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006840 Genomic DNA. Translation: BAD39142.1.
RefSeqiWP_011194292.1. NC_006177.1.
YP_073986.1. NC_006177.1.

3D structure databases

ProteinModelPortaliQ67T51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi292459.STH157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD39142; BAD39142; STH157.
GeneIDi2981020.
KEGGisth:STH157.
PATRICi23778373. VBISymThe116959_0158.

Phylogenomic databases

eggNOGiCOG0352.
HOGENOMiHOG000097679.
OMAiGANPERP.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.
BioCyciSTHE292459:GJMM-173-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
MF_00228. Thz_kinase.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
    Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
    Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T / IAM 14863.

Entry informationi

Entry nameiTHIME_SYMTH
AccessioniPrimary (citable) accession number: Q67T51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 11, 2004
Last modified: February 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.