Q67SK0 (CLPP1_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 1
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 1

Gene names

Name:	clpP1
Ordered Locus Names:	STH358

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000179679
Sites
	Active site	99	1	By similarity
	Active site	123	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q67SK0 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C232FAB363AF4342	FASTA	202	22,146
	10 20 30 40 50 60 MSYLIPYVIE QTNRGERSYD IYSRLLKDRI VFLGTPIDDQ VANVVIAQLL FLESEDPDKP 70 80 90 100 110 120 VNLYINSPGG SVSAGLGIYD AMQHIKSPVN TTVVGMAASM AALLLAAGTG KRYALPNARV 130 140 150 160 170 180 MIHQPHLSGV GGQVTDIEIT AREAVKTKQL MAEILAKHSG QSVERILADT ERDRWMSAQE 190 200 AVEYGLVDEV LHPREKASRS AR

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD39343.1.
RefSeq	YP_074187.1. NC_006177.1.
3D structure databases
ProteinModelPortal	Q67SK0.
SMR	Q67SK0. Positions 4-194.
ModBase	Search...
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2980335.
GenomeReviews	Gene locus STH358 in contig AP006840_GR.
KEGG	sth:STH358.
NMPDR	fig|292459.1.peg.338.
PATRIC	23778786. VBISymThe116959_0340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	NELYVKH.
ProtClustDB	CLSK737270.
Enzyme and pathway databases
BioCyc	STHE292459:STH358-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_SYMTH

Accession

Primary (citable) accession number: Q67SK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program