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Q67SB4 (SYL_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:STH444
OrganismSymbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]
Taxonomic identifier292459 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152101

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif588 – 5925"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5911ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q67SB4 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: CF32ACCC54E5F2F9

FASTA86197,335
        10         20         30         40         50         60 
MAEERFDFRE AEPRWQRRWD EEGIYKVERD LSRPKYYALA MFPYPSGKLH MGHVRNYTIV 

        70         80         90        100        110        120 
DVIARYRRMK GYNVLHPMGF DSFGMPAENA AIQHGANPAV WTRENIAEMT AQLKQMGYSY 

       130        140        150        160        170        180 
DWSRAVYTYR EDYYRWTQWL FLQFYKKGLA YKKTAPVNWC PSCQTVLANE QVEDGRCWRC 

       190        200        210        220        230        240 
DSVVTKKDLA QWFFRITQYA DELLEDLKLL EGGWPEQVRI MQQNWIGRSE GARVEFTLEA 

       250        260        270        280        290        300 
TGDKIPIFTT RPDTIYGVTF MVVAPEHPIV EKICTSGLIP EERVAAIRAF QEKMKHLSEI 

       310        320        330        340        350        360 
ARTSTEAEKE GLYTGLDVIN PFNGEKAQLW IANYVLMDYG TGAVMGVPAH DQRDFEFAQK 

       370        380        390        400        410        420 
YGLPVKVVIQ NPEGTLRAEE MTAAYVEPGI MVNSGPFDGT PNLEGIPKVI AYAEEQGFGQ 

       430        440        450        460        470        480 
KTVSYRLRDW LISRQRAWGA PIPIVYCDKC GTVPVPEKDL PVRLPDDLDF TGEGGSPLAR 

       490        500        510        520        530        540 
HEGFVNTTCP QCGGPARRET DTMDTFVCSS WYFLRYTDPQ NAERPWNRED VDYWMPVDQY 

       550        560        570        580        590        600 
VGGIEHAVLH LLYARFFTKV LRDMGLVKVD EPFARLLTQG MVLKDGSKMS KSKGNTVSPE 

       610        620        630        640        650        660 
EMIAKYGADA VRLFIMFAAP PERDLDWSDA GIEGAARFVN RFYRMVVSAL PAYQHARSLL 

       670        680        690        700        710        720 
PINPADPASV MGALSEAEIA EGLAKAAPNL TAEDRELRRV IHATVKRITA DLHDRFAFNT 

       730        740        750        760        770        780 
AISGLMEMTN AIYAYREKQH AEQNTSALVL AEAVQKAVLI IAPFCPHLAD ELWSRMGHPR 

       790        800        810        820        830        840 
SIHLEPWPAY DEEVAKADTV EIVVQINGRV RDRLEVPAGI SAAEMEAVAM ASEKVQALVA 

       850        860 
GKQIVKVVPV PGKLVNIVVK G 

« Hide

References

[1]"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T / IAM 14863.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006840 Genomic DNA. Translation: BAD39429.1.
RefSeqYP_074273.1. NC_006177.1.

3D structure databases

ProteinModelPortalQ67SB4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292459.STH444.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD39429; BAD39429; STH444.
GeneID2979842.
KEGGsth:STH444.
PATRIC23778962. VBISymThe116959_0428.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSTHE292459:GJMM-486-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYMTH
AccessionPrimary (citable) accession number: Q67SB4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries