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Q67S46 (Q67S46_SYMTH) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:STH512 EMBL BAD39497.1
OrganismSymbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]
Taxonomic identifier292459 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiales Family XVIII. Incertae SedisSymbiobacterium

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain144 – 350207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding177 – 23256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3031Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3191Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
Q67S46 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 2B4D0DD6FA097C87

FASTA36640,059
        10         20         30         40         50         60 
MCAKTRVYLL FGGRSGEHEV SLMSARNVLE ALDRSKYEVI PVGITKEGRW LQVGDPLRAL 

        70         80         90        100        110        120 
TEGVAAAGGR PVGLLGDPSY SGPVPLSDGL RPAAHDGPVV FFPVLHGTYG EDGTIQGLLE 

       130        140        150        160        170        180 
MANVPYVGCG VLASSVAMDK GVAKALFRQA GLEVGPYRVY LRRDWERHPE QIRADVSAHL 

       190        200        210        220        230        240 
RYPVFVKPAN LGSSVGITKV KRPEDLDRAM DEAARFDRRI IVEQGIDARE IEVAVLGNDD 

       250        260        270        280        290        300 
PIASVPGEIL PGAEFYDYTD KYFSGASQTR IPADLPDDLV EEFRRQAVVA FKAIDGSGMA 

       310        320        330        340        350        360 
RVDFFLERGT NRIFINEVNT IPGFTNISMY PKLWEASGIG YGELLDRLIQ LALERHADKN 


RNATSV 

« Hide

References

[1]"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism."
Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T.
Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T / IAM 14863.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006840 Genomic DNA. Translation: BAD39497.1.
RefSeqYP_074341.1. NC_006177.1.

3D structure databases

ProteinModelPortalQ67S46.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING292459.STH512.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD39497; BAD39497; STH512.
GeneID2982410.
KEGGsth:STH512.
PATRIC23779096. VBISymThe116959_0495.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycSTHE292459:GJMM-554-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ67S46_SYMTH
AccessionPrimary (citable) accession number: Q67S46
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)