Reviewed, UniProtKB/Swiss-Prot Q67RL6 (MURB1_SYMTH)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase 1
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase 1

Gene names

Name:	murB1
Ordered Locus Names:	STH692

Organism

Symbiobacterium thermophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2734 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable HAMAP MF_00037.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	UDP-N-acetylenolpyruvoylglucosamine reductase 1 HAMAP MF_00037		PRO_0000224728
Regions
	Domain	35 – 208	174	FAD-binding PCMH-type
Sites
	Active site	184	1	By similarity
	Active site	258	1	Proton donor By similarity
	Active site	356	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q67RL6-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 184A9A946B6032CE	FASTA	361	39,241
	10 20 30 40 50 60 MDVHGAAYAT HGKGVTGIAA LDIQQGVSLR DYSTMRLGGW AAYLAHVRSP AEVEEGIAWA 70 80 90 100 110 120 EARHLPVIMV GGGSNIIWRD EGFAGLVLVN RIPGFELADQ GGHLLLTVGA GENWDSVVAR 130 140 150 160 170 180 AVAAGASGIE RLSLIPGTAG ATPVQNVGAY GQEIADVLVS VDAYDRQERR FVRIPAAECA 190 200 210 220 230 240 FGYRRSRFNQ ADRGRFFITA LTLRLLREPP RAPFYPALGR YLEERGLTHP TVQQVRDAVI 250 260 270 280 290 300 AIRRAKLPDP AHVANCGSFF RNPIIPAPQA AELLRRYPDM PHWPVPGGGV KLAAGWLIDR 310 320 330 340 350 360 AGFRGVADPE TGMGTWPAQA LVVVNHRASS TADLLRFKQK VQDEVRRRFG VTLEQEPELL P

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed: 15383646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD39677.1.
RefSeq	YP_074521.1.
3D structure databases
SMR	Q67RL6. Positions 28-360.
ModBase	Search...
Genome annotation databases
GeneID	2981531.
GenomeReviews	Gene locus STH692 in contig AP006840_GR.
KEGG	sth:STH692.
NMPDR	fig|292459.1.peg.662.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686573.
OMA	WLIDQCG.
Enzyme and pathway databases
BioCyc	STHE292459:STH692-MONOMER.
BRENDA	1.1.1.158. 20615.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB1_SYMTH

Accession

Primary (citable) accession number: Q67RL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)